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The map label for this gene is dsbA [H]

Identifier: 85060214

GI number: 85060214

Start: 3892988

End: 3893611

Strand: Reverse

Name: dsbA [H]

Synonym: SG2236

Alternate gene names: 85060214

Gene position: 3893611-3892988 (Counterclockwise)

Preceding gene: 85060215

Following gene: 85060213

Centisome position: 93.35

GC content: 51.6

Gene sequence:

>624_bases
ATGAAAAAAATATGGTTTGCGTTGGTGGTTATCGCGTTGGCGTTCAGCGCCTCGGCCGCGCAATTTACTGAAGGTCAGCA
ATATGTCCAGCTCGACAAGCCGGTGACCAGTGAACCCCAAGTGCTGGAATTTTTCTCGTTTTATTGCGCGAATTGTTATC
AATTCGAGCAGGTCTATCACATCTCCAGCACGGTGAAAAAAGCGCTGCCTGCCGGCACCAAAATGACCAAATACCATGTG
GATTTTCTGGGGCCGATGGGCAAACAGCTGACCCAGGCCTGGGCCGTAGCCATGGCGCTGGGGGTGGAAGACAAAGTAAG
TCCGCTGCTGTTTGGTGGTCTGCAAAAAACGCAAACCATACAAACGCCGGACGACATCCGCGCGGTGTTTGTCAAAGCGG
GCGTCAGCGCGGAAGAGTATGATTCCGCCTGGAACAGCTTTGTCGTGAAATCTCTGGTCGTACAGCAGGAGAAAGCGGCC
ACCGATCTGCAGCTGCGCGGCGTGCCGGCGATGTTTGTCAACGGCAAATATATGGTGAAAAACGATGGGCTGGATATGTC
GTCAATGGACGCTTATACCAAACAATTTGCCGATGTCGTGAAATTTTTGCTTAGCCAAAAGTAA

Upstream 100 bases:

>100_bases
ATTGAACAGGCAACGCTGCTGCAACAGCCGCCGTTGCAGCTGATGCCGGTATATTAAACGTTTTATCGCCGATGTTATTC
ATTTTGGAGAGACACTATTT

Downstream 100 bases:

>100_bases
ACTCTCCTGCGGGTGAATGACGTACAACGCCGGCAGATGCCGGCGTTGTCGTTTGTGGCGAAGCCGCAAACAGCCAGTAT
CTCCTTAATCTGCCGAAGGA

Product: periplasmic protein disulfide isomerase I

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MKKIWFALVVIALAFSASAAQFTEGQQYVQLDKPVTSEPQVLEFFSFYCANCYQFEQVYHISSTVKKALPAGTKMTKYHV
DFLGPMGKQLTQAWAVAMALGVEDKVSPLLFGGLQKTQTIQTPDDIRAVFVKAGVSAEEYDSAWNSFVVKSLVVQQEKAA
TDLQLRGVPAMFVNGKYMVKNDGLDMSSMDAYTKQFADVVKFLLSQK

Sequences:

>Translated_207_residues
MKKIWFALVVIALAFSASAAQFTEGQQYVQLDKPVTSEPQVLEFFSFYCANCYQFEQVYHISSTVKKALPAGTKMTKYHV
DFLGPMGKQLTQAWAVAMALGVEDKVSPLLFGGLQKTQTIQTPDDIRAVFVKAGVSAEEYDSAWNSFVVKSLVVQQEKAA
TDLQLRGVPAMFVNGKYMVKNDGLDMSSMDAYTKQFADVVKFLLSQK
>Mature_207_residues
MKKIWFALVVIALAFSASAAQFTEGQQYVQLDKPVTSEPQVLEFFSFYCANCYQFEQVYHISSTVKKALPAGTKMTKYHV
DFLGPMGKQLTQAWAVAMALGVEDKVSPLLFGGLQKTQTIQTPDDIRAVFVKAGVSAEEYDSAWNSFVVKSLVVQQEKAA
TDLQLRGVPAMFVNGKYMVKNDGLDMSSMDAYTKQFADVVKFLLSQK

Specific function: Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. It is required for pilus biogenesis [H]

COG id: COG0526

COG function: function code OC; Thiol-disulfide isomerase and thioredoxins

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=208, Percent_Identity=67.3076923076923, Blast_Score=295, Evalue=2e-81,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 22998; Mature: 22998

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIWFALVVIALAFSASAAQFTEGQQYVQLDKPVTSEPQVLEFFSFYCANCYQFEQVYH
CHHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ISSTVKKALPAGTKMTKYHVDFLGPMGKQLTQAWAVAMALGVEDKVSPLLFGGLQKTQTI
HHHHHHHHCCCCCCHHEEHHHHHCHHHHHHHHHHHHHHHHCCCHHCCHHHHCCCCCCCCC
QTPDDIRAVFVKAGVSAEEYDSAWNSFVVKSLVVQQEKAATDLQLRGVPAMFVNGKYMVK
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEECCEEEEE
NDGLDMSSMDAYTKQFADVVKFLLSQK
CCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKIWFALVVIALAFSASAAQFTEGQQYVQLDKPVTSEPQVLEFFSFYCANCYQFEQVYH
CHHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHH
ISSTVKKALPAGTKMTKYHVDFLGPMGKQLTQAWAVAMALGVEDKVSPLLFGGLQKTQTI
HHHHHHHHCCCCCCHHEEHHHHHCHHHHHHHHHHHHHHHHCCCHHCCHHHHCCCCCCCCC
QTPDDIRAVFVKAGVSAEEYDSAWNSFVVKSLVVQQEKAATDLQLRGVPAMFVNGKYMVK
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCEEEECCEEEEE
NDGLDMSSMDAYTKQFADVVKFLLSQK
CCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10463161 [H]