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The map label for this gene is dusA [H]

Identifier: 85060114

GI number: 85060114

Start: 3709410

End: 3710441

Strand: Reverse

Name: dusA [H]

Synonym: SG2136

Alternate gene names: 85060114

Gene position: 3710441-3709410 (Counterclockwise)

Preceding gene: 85060116

Following gene: 85060112

Centisome position: 88.95

GC content: 55.91

Gene sequence:

>1032_bases
ATGCTTGAAAACCGCTTTCAACAGAACAGCCAGCCGTCCACTTTAATCTCGGCAGGGCCTATAACGCTTGCACCAGGCCG
ATTCAGCGTTGCACCTATGCTGGATTGGACCGATAGTCATTGCCGTTTTTTTCTGCGCCAGCTAAGTCGCCGCGCGCTGT
TGTACACGGAAATGGTGACCACCGGAGCTTTATTGTTTGGCAAAGGCGATTATTTGGCCTACCACGACGCGGAACACCCC
CTGGCGCTGCAACTGGGCGGTAGCGAACCTGATGCGCTGGCGCGTTGTGCCAAGTTGGCACAAGAGCGAGGCTATGATGA
AATCAACCTCAATGTAGGCTGCCCCTCCGATCGGGTGCAGAACGGTCGTTTCGGCGCTTGCCTGATGGCTGAAAAGGACC
GGGTGGCGGCCTGTATTGCCGCTATGCGCGAGGCGGTGACGCTGCCGATTACGGTTAAAACCCGCATCGGTATTGACGAG
CAGGACAGCTACGCGTTTTTGTGTGAATTTATCGATACCGTCGCCACGCACGGCGGATGCGAAACATTCATAATTCACGC
CCGTAAGGCATGGCTCAATGGCCTGAGCCCCAAAGAGAACCGCGAAATTCCGCCGCTGGATTATGATCGCGTGTATCAGC
TGAAACGGGATTTTCCCGCGCTCCGATTGATGCTTAATGGCGGGATTAAAACGCTGCACGACGCACGGGAACATTTGCAA
AAGCTTGATGGTGTGATGATGGGGCGGGAAGCCTACCAAAACCCGGGCATCCTGGCGCGGGTCGACAGTGAACTGTTTGG
CGATGCCGATACGTTGCCGAATCAGGCGGCAGTGATTGATGTGATGTTGCCTTATATCGAGCGCGAGCTGTCCCAAGGCG
CAGCGCTGGGTCACATTACCCGTCACATGCTTGGACTGTTTCAGGGAATGCCGGGCGCCCGGCAGTGGCGCCGCTATTTG
AGCGAGCACGCTCACCGACCTGGCGCAGGTATCGATGTGGTTATTAAAGCGCTATCTTGCATCCAGCATTAA

Upstream 100 bases:

>100_bases
TTTTCTGTGTTAGAATCTAGCGGAAGTTTCCCTGCCGGTGCCCTCTTTCACTGTGCTGAACGGGGATTTAGTTGCTTGAA
CAGACGTGAAACTATCAGCC

Downstream 100 bases:

>100_bases
TAACTGGCTTAGCGGTTTCAGAAATTTTTGCGGCACATAACGGTCAAAACCGGTGTGCCTACTGTATTCGCTGAACTGCC
GTAAACAGTGTACGCCCGCT

Product: tRNA-dihydrouridine synthase A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MLENRFQQNSQPSTLISAGPITLAPGRFSVAPMLDWTDSHCRFFLRQLSRRALLYTEMVTTGALLFGKGDYLAYHDAEHP
LALQLGGSEPDALARCAKLAQERGYDEINLNVGCPSDRVQNGRFGACLMAEKDRVAACIAAMREAVTLPITVKTRIGIDE
QDSYAFLCEFIDTVATHGGCETFIIHARKAWLNGLSPKENREIPPLDYDRVYQLKRDFPALRLMLNGGIKTLHDAREHLQ
KLDGVMMGREAYQNPGILARVDSELFGDADTLPNQAAVIDVMLPYIERELSQGAALGHITRHMLGLFQGMPGARQWRRYL
SEHAHRPGAGIDVVIKALSCIQH

Sequences:

>Translated_343_residues
MLENRFQQNSQPSTLISAGPITLAPGRFSVAPMLDWTDSHCRFFLRQLSRRALLYTEMVTTGALLFGKGDYLAYHDAEHP
LALQLGGSEPDALARCAKLAQERGYDEINLNVGCPSDRVQNGRFGACLMAEKDRVAACIAAMREAVTLPITVKTRIGIDE
QDSYAFLCEFIDTVATHGGCETFIIHARKAWLNGLSPKENREIPPLDYDRVYQLKRDFPALRLMLNGGIKTLHDAREHLQ
KLDGVMMGREAYQNPGILARVDSELFGDADTLPNQAAVIDVMLPYIERELSQGAALGHITRHMLGLFQGMPGARQWRRYL
SEHAHRPGAGIDVVIKALSCIQH
>Mature_343_residues
MLENRFQQNSQPSTLISAGPITLAPGRFSVAPMLDWTDSHCRFFLRQLSRRALLYTEMVTTGALLFGKGDYLAYHDAEHP
LALQLGGSEPDALARCAKLAQERGYDEINLNVGCPSDRVQNGRFGACLMAEKDRVAACIAAMREAVTLPITVKTRIGIDE
QDSYAFLCEFIDTVATHGGCETFIIHARKAWLNGLSPKENREIPPLDYDRVYQLKRDFPALRLMLNGGIKTLHDAREHLQ
KLDGVMMGREAYQNPGILARVDSELFGDADTLPNQAAVIDVMLPYIERELSQGAALGHITRHMLGLFQGMPGARQWRRYL
SEHAHRPGAGIDVVIKALSCIQH

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusA subfamily [H]

Homologues:

Organism=Homo sapiens, GI40807366, Length=249, Percent_Identity=27.3092369477912, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI239788483, Length=222, Percent_Identity=26.1261261261261, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI239788462, Length=222, Percent_Identity=26.1261261261261, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI145693211, Length=316, Percent_Identity=73.7341772151899, Blast_Score=499, Evalue=1e-143,
Organism=Escherichia coli, GI1789660, Length=247, Percent_Identity=25.5060728744939, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=223, Percent_Identity=26.0089686098655, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323560, Length=246, Percent_Identity=25.2032520325203, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24580595, Length=316, Percent_Identity=24.0506329113924, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI19920448, Length=316, Percent_Identity=24.0506329113924, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004653
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: 1.-.-.-

Molecular weight: Translated: 38155; Mature: 38155

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENRFQQNSQPSTLISAGPITLAPGRFSVAPMLDWTDSHCRFFLRQLSRRALLYTEMVT
CCCCHHCCCCCCCEEEECCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TGALLFGKGDYLAYHDAEHPLALQLGGSEPDALARCAKLAQERGYDEINLNVGCPSDRVQ
HCEEEEECCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHC
NGRFGACLMAEKDRVAACIAAMREAVTLPITVKTRIGIDEQDSYAFLCEFIDTVATHGGC
CCCCCEEEECCCHHHHHHHHHHHHHEECEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
ETFIIHARKAWLNGLSPKENREIPPLDYDRVYQLKRDFPALRLMLNGGIKTLHDAREHLQ
HHEEEEHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHH
KLDGVMMGREAYQNPGILARVDSELFGDADTLPNQAAVIDVMLPYIERELSQGAALGHIT
HHHHHHHHHHHHCCCCEEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
RHMLGLFQGMPGARQWRRYLSEHAHRPGAGIDVVIKALSCIQH
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MLENRFQQNSQPSTLISAGPITLAPGRFSVAPMLDWTDSHCRFFLRQLSRRALLYTEMVT
CCCCHHCCCCCCCEEEECCCEEECCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
TGALLFGKGDYLAYHDAEHPLALQLGGSEPDALARCAKLAQERGYDEINLNVGCPSDRVQ
HCEEEEECCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCHHHHC
NGRFGACLMAEKDRVAACIAAMREAVTLPITVKTRIGIDEQDSYAFLCEFIDTVATHGGC
CCCCCEEEECCCHHHHHHHHHHHHHEECEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCC
ETFIIHARKAWLNGLSPKENREIPPLDYDRVYQLKRDFPALRLMLNGGIKTLHDAREHLQ
HHEEEEHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCHHHHHHHHHHHH
KLDGVMMGREAYQNPGILARVDSELFGDADTLPNQAAVIDVMLPYIERELSQGAALGHIT
HHHHHHHHHHHHCCCCEEEEECHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
RHMLGLFQGMPGARQWRRYLSEHAHRPGAGIDVVIKALSCIQH
HHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]