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The map label for this gene is spaP

Identifier: 85060062

GI number: 85060062

Start: 3589615

End: 3590316

Strand: Reverse

Name: spaP

Synonym: SG2084

Alternate gene names: 85060062

Gene position: 3590316-3589615 (Counterclockwise)

Preceding gene: 85060063

Following gene: 85060061

Centisome position: 86.08

GC content: 56.41

Gene sequence:

>702_bases
ATGACCGCTAATCTCGACCTGCCCAACGACATGGGACTTATCGGGATTCTGGCACTGGCAACCCTATTGCCTTTTATCAT
TGCCTCCGGCACCTGCTATATCAAATTCTCCATCGTCTTCGTTATGGTGCGCAACGCTCTGGGCCTGCAGCAGGTGCCTT
CCAACCTTACTCTGAACGGTATCGCGCTGATGCTGGCGATGTTTATCATGGGGCCTATCGCCGGCGATGCCTATAATGCC
TACCAGGAGCAGCCGGTGGCGTTGGGCAGCGCCGCCTCGGTGCTGGAATTTCTGGAAAACAGCATGGAGTCCTACCGGCA
TTATCTGCAGAAATACGCCGACCCGCGCCTGACTGAATTCTTCGATCGGGCTCACGGTGGCGGCGGTGCTGCGCATGACG
CACCCGCGTCCGGTCATGCGCCCTCCATGTTGGCGCTATTGCCTGCCTATGCGCTAAGTGAAATCAAGGACTCCTTCAGA
ATCGGCTTTTATCTGTATCTTCCCTTTGTGGTGGTGGACCTGCTGATTTCCAGCGTTTTGCTGGCGTTGGGCATGATGAT
GATGAGCCCGATTACCCTGTCGGTGCCGATAAAATTGGTGTTGTTCGTCATGCTCGACGGTTGGACGCTGCTATCGCAAG
GGCTGGTTCTGCAATATCTCGATATGGGTGCCGGCGCGGAGATTGCGGGGGGCCCGGGATGA

Upstream 100 bases:

>100_bases
GAGATTCGCGCCAATGGCCATCTGCTGGCGCTCGGCGAACTGGTACAACTCGAAGATCGTCTGGGCGTGGAATTGATGGA
GTTGTCGCTGGATAACCGTT

Downstream 100 bases:

>100_bases
ACAATTTGTTATTTGCCGGCAATAAAGCCCTGTATCTGGTACTGATGATGGCAGCGGGGCCTATTATTGTGGCGACGCTG
GTGGGATTGCTGGTGGGGCT

Product: surface presentation of antigens protein SpaP

Products: NA

Alternate protein names: Spa24 protein [H]

Number of amino acids: Translated: 233; Mature: 232

Protein sequence:

>233_residues
MTANLDLPNDMGLIGILALATLLPFIIASGTCYIKFSIVFVMVRNALGLQQVPSNLTLNGIALMLAMFIMGPIAGDAYNA
YQEQPVALGSAASVLEFLENSMESYRHYLQKYADPRLTEFFDRAHGGGGAAHDAPASGHAPSMLALLPAYALSEIKDSFR
IGFYLYLPFVVVDLLISSVLLALGMMMMSPITLSVPIKLVLFVMLDGWTLLSQGLVLQYLDMGAGAEIAGGPG

Sequences:

>Translated_233_residues
MTANLDLPNDMGLIGILALATLLPFIIASGTCYIKFSIVFVMVRNALGLQQVPSNLTLNGIALMLAMFIMGPIAGDAYNA
YQEQPVALGSAASVLEFLENSMESYRHYLQKYADPRLTEFFDRAHGGGGAAHDAPASGHAPSMLALLPAYALSEIKDSFR
IGFYLYLPFVVVDLLISSVLLALGMMMMSPITLSVPIKLVLFVMLDGWTLLSQGLVLQYLDMGAGAEIAGGPG
>Mature_232_residues
TANLDLPNDMGLIGILALATLLPFIIASGTCYIKFSIVFVMVRNALGLQQVPSNLTLNGIALMLAMFIMGPIAGDAYNAY
QEQPVALGSAASVLEFLENSMESYRHYLQKYADPRLTEFFDRAHGGGGAAHDAPASGHAPSMLALLPAYALSEIKDSFRI
GFYLYLPFVVVDLLISSVLLALGMMMMSPITLSVPIKLVLFVMLDGWTLLSQGLVLQYLDMGAGAEIAGGPG

Specific function: Required for surface presentation of invasion plasmid antigens. Could play a role in preserving the translocation competence of the ipa antigens. Required for invasion and for secretion of the three ipa proteins [H]

COG id: COG4790

COG function: function code U; Type III secretory pathway, component EscR

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliP/mopC/spaP family [H]

Homologues:

Organism=Escherichia coli, GI1788259, Length=196, Percent_Identity=39.2857142857143, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005838
- InterPro:   IPR005773 [H]

Pfam domain/function: PF00813 FliP [H]

EC number: NA

Molecular weight: Translated: 24911; Mature: 24780

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS01061 FLIP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
6.0 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.6 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTANLDLPNDMGLIGILALATLLPFIIASGTCYIKFSIVFVMVRNALGLQQVPSNLTLNG
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHCCCCCCHHH
IALMLAMFIMGPIAGDAYNAYQEQPVALGSAASVLEFLENSMESYRHYLQKYADPRLTEF
HHHHHHHHHHCCCCCCHHHHHHCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FDRAHGGGGAAHDAPASGHAPSMLALLPAYALSEIKDSFRIGFYLYLPFVVVDLLISSVL
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALGMMMMSPITLSVPIKLVLFVMLDGWTLLSQGLVLQYLDMGAGAEIAGGPG
HHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TANLDLPNDMGLIGILALATLLPFIIASGTCYIKFSIVFVMVRNALGLQQVPSNLTLNG
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHCCCCCCHHH
IALMLAMFIMGPIAGDAYNAYQEQPVALGSAASVLEFLENSMESYRHYLQKYADPRLTEF
HHHHHHHHHHCCCCCCHHHHHHCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
FDRAHGGGGAAHDAPASGHAPSMLALLPAYALSEIKDSFRIGFYLYLPFVVVDLLISSVL
HHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LALGMMMMSPITLSVPIKLVLFVMLDGWTLLSQGLVLQYLDMGAGAEIAGGPG
HHHHHHHHCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1312536; 8385666; 11115111; 11292750; 12384590 [H]