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The map label for this gene is pgk

Identifier: 85059993

GI number: 85059993

Start: 3473274

End: 3474437

Strand: Reverse

Name: pgk

Synonym: SG2015

Alternate gene names: 85059993

Gene position: 3474437-3473274 (Counterclockwise)

Preceding gene: 85059995

Following gene: 85059992

Centisome position: 83.3

GC content: 55.84

Gene sequence:

>1164_bases
ATGGCTGTAATTAAGATGACCGATCTGGATCTGGCTGGTAAACGTGTATTTATCCGTTCCGATCTGAACGTGCCGGTTAA
AGACGGGAAAGTAACGTCCGATGCGCGTATCCGCGCCTCTCTACCTACTATCGAAGACTCACTGAAACAGGGCGCGCGCG
TCATGGTGACCTCCCATCTCGGCCGCCCGACTGAAGGGGAATATAACGCCGAGTTTTCGCTGCAGCCGGTGGTGGATTAT
CTCGAAAATAAACTCTCCGCGCCGGTGCGTCTGGCCAAAGATTACCTTGATGGCGTAGATGTCGCCGAGGGCGAACTGGT
CGTGCTGGAAAACGTGCGCTTCAACAAGGGCGAAAAGAAAGATGACGAAGCCCTGGCGAAGAAATATGCCGCGCTGTGCG
ACGTGTTCGTCATGGATGCCTTCGGTACCGCCCACCGCGCTCAGGCCTCCACCCACGGCGTAGGTAAATTCGCGGCCGTC
GCTTGCGCCGGCCCGCTGCTATTCAATGAGCTGGAAGCGCTGGGCAAAGCGCTTGATAACCCGGCCCGGCCGATGGTGGC
CATCGTTGGCGGTTCAAAAGTCTCCACCAAGCTGACTGTATTGGATTCACTGTCGAAAATTGCCGATCAGCTCATCGTCG
GCGGCGGCATCGCCAACACCTTCGTTGCGGCGCAGGGGCATAACGTCGGTAAATCGCTCTACGAAGCCGACCTGATCTCG
GAAGCCAAACGCCTGCTGGAAAGCAGCGATATTCCCGTACCGACCGATGTTCGCGTCGCTACCGAATTTTCTGAAACCGC
GACCGCAACCTTGAAATCCACCAGCGAAATTCAAGACAGTGAACAGATTCTGGATTTGGGCGATGTTTCCGTCGCCCGTC
TGGCGGAAATTCTGAAAAACGCCAAAACCATTTTGTGGAATGGTCCGGTCGGCGTGTTCGAGTTCGCCAATTTCCGTAAA
GGCACCGAGGTGGTAGCCCGCGCTATCGCCGATAGCGAGGCATTCTCTATAGCCGGCGGCGGAGATACCCTGGCGGCCAT
CGACCTGTTTGGCATTGCCGATCAAATCTCCTATATTTCCACTGGCGGCGGCGCTTTCCTTGAATTTGTCGAAGGCAAGA
AACTTCCCGCGGTGGTCATGCTTGAAGAACGCGCGAAACAATAA

Upstream 100 bases:

>100_bases
CATTGAATGGACGGGGTCACAAGCGACTATTGGTCCGGTGTGAGGAAGCCTTTCCCCGCCGCTCAGGCAATTTAGCAAGC
ATCAAACAAGAGGGTTCACC

Downstream 100 bases:

>100_bases
GTATTGAGCGGGCGCACGCCACCGGTGTTCGCCGCGGCTGGTACACAGGCTCGGGCCGCGGTGAGAAGTCACCCCTTTTA
ATGGCCTACAAAACAGGACA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 387; Mature: 386

Protein sequence:

>387_residues
MAVIKMTDLDLAGKRVFIRSDLNVPVKDGKVTSDARIRASLPTIEDSLKQGARVMVTSHLGRPTEGEYNAEFSLQPVVDY
LENKLSAPVRLAKDYLDGVDVAEGELVVLENVRFNKGEKKDDEALAKKYAALCDVFVMDAFGTAHRAQASTHGVGKFAAV
ACAGPLLFNELEALGKALDNPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFVAAQGHNVGKSLYEADLIS
EAKRLLESSDIPVPTDVRVATEFSETATATLKSTSEIQDSEQILDLGDVSVARLAEILKNAKTILWNGPVGVFEFANFRK
GTEVVARAIADSEAFSIAGGGDTLAAIDLFGIADQISYISTGGGAFLEFVEGKKLPAVVMLEERAKQ

Sequences:

>Translated_387_residues
MAVIKMTDLDLAGKRVFIRSDLNVPVKDGKVTSDARIRASLPTIEDSLKQGARVMVTSHLGRPTEGEYNAEFSLQPVVDY
LENKLSAPVRLAKDYLDGVDVAEGELVVLENVRFNKGEKKDDEALAKKYAALCDVFVMDAFGTAHRAQASTHGVGKFAAV
ACAGPLLFNELEALGKALDNPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFVAAQGHNVGKSLYEADLIS
EAKRLLESSDIPVPTDVRVATEFSETATATLKSTSEIQDSEQILDLGDVSVARLAEILKNAKTILWNGPVGVFEFANFRK
GTEVVARAIADSEAFSIAGGGDTLAAIDLFGIADQISYISTGGGAFLEFVEGKKLPAVVMLEERAKQ
>Mature_386_residues
AVIKMTDLDLAGKRVFIRSDLNVPVKDGKVTSDARIRASLPTIEDSLKQGARVMVTSHLGRPTEGEYNAEFSLQPVVDYL
ENKLSAPVRLAKDYLDGVDVAEGELVVLENVRFNKGEKKDDEALAKKYAALCDVFVMDAFGTAHRAQASTHGVGKFAAVA
CAGPLLFNELEALGKALDNPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFVAAQGHNVGKSLYEADLISE
AKRLLESSDIPVPTDVRVATEFSETATATLKSTSEIQDSEQILDLGDVSVARLAEILKNAKTILWNGPVGVFEFANFRKG
TEVVARAIADSEAFSIAGGGDTLAAIDLFGIADQISYISTGGGAFLEFVEGKKLPAVVMLEERAKQ

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=410, Percent_Identity=36.5853658536585, Blast_Score=231, Evalue=1e-60,
Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=36.0097323600973, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI1789294, Length=387, Percent_Identity=87.0801033591731, Blast_Score=683, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17508823, Length=403, Percent_Identity=41.6873449131514, Blast_Score=268, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6319857, Length=412, Percent_Identity=39.5631067961165, Blast_Score=251, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24581203, Length=415, Percent_Identity=38.3132530120482, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI17136394, Length=411, Percent_Identity=38.4428223844282, Blast_Score=234, Evalue=8e-62,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41144; Mature: 41013

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIKMTDLDLAGKRVFIRSDLNVPVKDGKVTSDARIRASLPTIEDSLKQGARVMVTSHL
CEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEEEEEECC
GRPTEGEYNAEFSLQPVVDYLENKLSAPVRLAKDYLDGVDVAEGELVVLENVRFNKGEKK
CCCCCCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCC
DDEALAKKYAALCDVFVMDAFGTAHRAQASTHGVGKFAAVACAGPLLFNELEALGKALDN
HHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFVAAQGHNVGKSLYEADLIS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEECCCHHHHEEEECCCCHHHHHHHHHHHH
EAKRLLESSDIPVPTDVRVATEFSETATATLKSTSEIQDSEQILDLGDVSVARLAEILKN
HHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHCHHHHCCHHHHHCCCCCCHHHHHHHHHC
AKTILWNGPVGVFEFANFRKGTEVVARAIADSEAFSIAGGGDTLAAIDLFGIADQISYIS
CCEEEECCCCCHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHEE
TGGGAFLEFVEGKKLPAVVMLEERAKQ
CCCHHHHHHHCCCCCCEEEEEHHHCCC
>Mature Secondary Structure 
AVIKMTDLDLAGKRVFIRSDLNVPVKDGKVTSDARIRASLPTIEDSLKQGARVMVTSHL
EEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCHHHHHHHCCCEEEEEECC
GRPTEGEYNAEFSLQPVVDYLENKLSAPVRLAKDYLDGVDVAEGELVVLENVRFNKGEKK
CCCCCCCCCCEEEHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCC
DDEALAKKYAALCDVFVMDAFGTAHRAQASTHGVGKFAAVACAGPLLFNELEALGKALDN
HHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
PARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIANTFVAAQGHNVGKSLYEADLIS
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHEECCCHHHHEEEECCCCHHHHHHHHHHHH
EAKRLLESSDIPVPTDVRVATEFSETATATLKSTSEIQDSEQILDLGDVSVARLAEILKN
HHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHCHHHHCCHHHHHCCCCCCHHHHHHHHHC
AKTILWNGPVGVFEFANFRKGTEVVARAIADSEAFSIAGGGDTLAAIDLFGIADQISYIS
CCEEEECCCCCHHHHHCCCCHHHHHHHHHHCCCCEEEECCCCCEEHHHHHHHHHHHHHEE
TGGGAFLEFVEGKKLPAVVMLEERAKQ
CCCHHHHHHHCCCCCCEEEEEHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA