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The map label for this gene is fba [H]

Identifier: 85059992

GI number: 85059992

Start: 3472090

End: 3473166

Strand: Reverse

Name: fba [H]

Synonym: SG2014

Alternate gene names: 85059992

Gene position: 3473166-3472090 (Counterclockwise)

Preceding gene: 85059993

Following gene: 85059991

Centisome position: 83.27

GC content: 54.13

Gene sequence:

>1077_bases
ATGTCTAAGATTTTCGATTTTGTTAAACCAGGTGTCATCACCGGTGATGACGTTCAGAAAGTGTTCGCTGTCGCGAAAGA
AAACAACTTTGCTCTGCCCGCTGTCAACTGTGTAGGCACCGATTCCATTAACGCCGCGCTGGAAGCGGCTGCCAAGGTGC
GCGCTCCGATTATCGTGCAATTTTCCAACGGCGGCGCCGCCTTTATCGCGGGCAAAGGCTTGAAAGCCGAAGGTCAGACT
GCGGCCGTCCTGGGCGCGGTGTCCGGCGCGCTGCATGTGCACCATATGGCCGAGCATTACGGCATCCCCGTTATTCTGCA
TACCGACCATTGCGCCAAGAAGCTGCTGCCGTGGCTTGACGGACTGCTGGACGCCGGCGAGAAACATTATGCCGCCACCT
GCAAGCCGCTTTTCTCATCCCATATGATCGATCTGTCCGAAGAGTCGCTTGAAGAGAACATCGAAATCAGCGCGAAATAT
CTGACGCGTATGGCCAAGCTGGATATGACGCTGGAAATCGAGCTGGGTTGCACTGGCGGTGAAGAAGACGGCGTAGACAA
CAGCCATCTGGATAACTCCGCGCTGTACACCCAGCCGGAAGATGTCGCCTATGCCTATGAAAAACTACATGCCATCAGCC
CGCGTTTCACCATTGCGGCCGCGTTCGGCAACGTTCATGGCGTTTACAAGCCGGGTAACGTGCAGCTGACGCCAAAAATT
CTGGATAATTCGCAAAAATACGTTTCGGAAAAATTTGGTCTGCCGGCCAAATCCCTGAATTTGGTCTTCCACGGCGGTTC
AGGTTCTACGCCGGAAGAAATCAAGGAAGCCGTCAGCTACGGCGTGGTTAAAATGAATATCGATACTGATACTCAGTGGG
CCACCTGGGAAGGGATTCTGAAATATTACCAGAAGAACGAAGGTTACCTGCGGGCACAGCTGGGCAACCCGGAAGGCGCC
GATAAACCCAACAAGAAGTTCTACGATCCGCGTGTGTGGATCCGCGCCGGTCAAGCCTCCATGGCGACGCGCCTGGAGCT
GGCCTTTAAAGAACTGAACGCTATCGACGTACTGTAA

Upstream 100 bases:

>100_bases
GCGGGCGCACGCCACCGGTGTTCGCCGCGGCTGGTACACAGGCTCGGGCCGCGGTGAGAAGTCACCCCTTTTAATGGCCT
ACAAAACAGGACAAAGTAAT

Downstream 100 bases:

>100_bases
CTGAGTGGCCCCACACCGCGCGTAGCGCGGTGTGGGGCCATGTGTTCCCTCCCGCCGTGATGCGGGTTGGACGTTAATCG
CCGCACTATCCACCGGAAAG

Product: fructose-bisphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSKIFDFVKPGVITGDDVQKVFAVAKENNFALPAVNCVGTDSINAALEAAAKVRAPIIVQFSNGGAAFIAGKGLKAEGQT
AAVLGAVSGALHVHHMAEHYGIPVILHTDHCAKKLLPWLDGLLDAGEKHYAATCKPLFSSHMIDLSEESLEENIEISAKY
LTRMAKLDMTLEIELGCTGGEEDGVDNSHLDNSALYTQPEDVAYAYEKLHAISPRFTIAAAFGNVHGVYKPGNVQLTPKI
LDNSQKYVSEKFGLPAKSLNLVFHGGSGSTPEEIKEAVSYGVVKMNIDTDTQWATWEGILKYYQKNEGYLRAQLGNPEGA
DKPNKKFYDPRVWIRAGQASMATRLELAFKELNAIDVL

Sequences:

>Translated_358_residues
MSKIFDFVKPGVITGDDVQKVFAVAKENNFALPAVNCVGTDSINAALEAAAKVRAPIIVQFSNGGAAFIAGKGLKAEGQT
AAVLGAVSGALHVHHMAEHYGIPVILHTDHCAKKLLPWLDGLLDAGEKHYAATCKPLFSSHMIDLSEESLEENIEISAKY
LTRMAKLDMTLEIELGCTGGEEDGVDNSHLDNSALYTQPEDVAYAYEKLHAISPRFTIAAAFGNVHGVYKPGNVQLTPKI
LDNSQKYVSEKFGLPAKSLNLVFHGGSGSTPEEIKEAVSYGVVKMNIDTDTQWATWEGILKYYQKNEGYLRAQLGNPEGA
DKPNKKFYDPRVWIRAGQASMATRLELAFKELNAIDVL
>Mature_357_residues
SKIFDFVKPGVITGDDVQKVFAVAKENNFALPAVNCVGTDSINAALEAAAKVRAPIIVQFSNGGAAFIAGKGLKAEGQTA
AVLGAVSGALHVHHMAEHYGIPVILHTDHCAKKLLPWLDGLLDAGEKHYAATCKPLFSSHMIDLSEESLEENIEISAKYL
TRMAKLDMTLEIELGCTGGEEDGVDNSHLDNSALYTQPEDVAYAYEKLHAISPRFTIAAAFGNVHGVYKPGNVQLTPKIL
DNSQKYVSEKFGLPAKSLNLVFHGGSGSTPEEIKEAVSYGVVKMNIDTDTQWATWEGILKYYQKNEGYLRAQLGNPEGAD
KPNKKFYDPRVWIRAGQASMATRLELAFKELNAIDVL

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis [H]

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1789293, Length=359, Percent_Identity=81.3370473537605, Blast_Score=617, Evalue=1e-178,
Organism=Escherichia coli, GI1789526, Length=317, Percent_Identity=27.1293375394322, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87082041, Length=318, Percent_Identity=27.0440251572327, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788072, Length=272, Percent_Identity=25.7352941176471, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322790, Length=351, Percent_Identity=51.8518518518518, Blast_Score=378, Evalue=1e-106,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006411
- InterPro:   IPR000771 [H]

Pfam domain/function: PF01116 F_bP_aldolase [H]

EC number: =4.1.2.13 [H]

Molecular weight: Translated: 38866; Mature: 38734

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00602 ALDOLASE_CLASS_II_1 ; PS00806 ALDOLASE_CLASS_II_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKIFDFVKPGVITGDDVQKVFAVAKENNFALPAVNCVGTDSINAALEAAAKVRAPIIVQ
CCCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEE
FSNGGAAFIAGKGLKAEGQTAAVLGAVSGALHVHHMAEHYGIPVILHTDHCAKKLLPWLD
ECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHH
GLLDAGEKHYAATCKPLFSSHMIDLSEESLEENIEISAKYLTRMAKLDMTLEIELGCTGG
HHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCEEHHHHHHHHHHCCEEEEEEEECCCC
EEDGVDNSHLDNSALYTQPEDVAYAYEKLHAISPRFTIAAAFGNVHGVYKPGNVQLTPKI
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCEEECHHH
LDNSQKYVSEKFGLPAKSLNLVFHGGSGSTPEEIKEAVSYGVVKMNIDTDTQWATWEGIL
HCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHH
KYYQKNEGYLRAQLGNPEGADKPNKKFYDPRVWIRAGQASMATRLELAFKELNAIDVL
HHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKIFDFVKPGVITGDDVQKVFAVAKENNFALPAVNCVGTDSINAALEAAAKVRAPIIVQ
CCHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEE
FSNGGAAFIAGKGLKAEGQTAAVLGAVSGALHVHHMAEHYGIPVILHTDHCAKKLLPWLD
ECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHH
GLLDAGEKHYAATCKPLFSSHMIDLSEESLEENIEISAKYLTRMAKLDMTLEIELGCTGG
HHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHCCCEEHHHHHHHHHHCCEEEEEEEECCCC
EEDGVDNSHLDNSALYTQPEDVAYAYEKLHAISPRFTIAAAFGNVHGVYKPGNVQLTPKI
CCCCCCCCCCCCCEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCEEECHHH
LDNSQKYVSEKFGLPAKSLNLVFHGGSGSTPEEIKEAVSYGVVKMNIDTDTQWATWEGIL
HCCCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHCCEEEEECCCCCCHHHHHHHH
KYYQKNEGYLRAQLGNPEGADKPNKKFYDPRVWIRAGQASMATRLELAFKELNAIDVL
HHHHCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12542086 [H]