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The map label for this gene is amiC [H]

Identifier: 85059939

GI number: 85059939

Start: 3375471

End: 3376730

Strand: Reverse

Name: amiC [H]

Synonym: SG1961

Alternate gene names: 85059939

Gene position: 3376730-3375471 (Counterclockwise)

Preceding gene: 85059940

Following gene: 85059938

Centisome position: 80.95

GC content: 58.49

Gene sequence:

>1260_bases
ATGCCTGAGTCAGAACATAACCGCGCTCGCCGGCTGCTGCTGCAAGGCGTGGCGGCCACCTGGCTGTTAAGCGTCAGCCG
GGTTGGCCTCGCCGCTGCGCCTTCTATTGTGGCGGTGCGGATCTGGCCCGCCTCAAGCTATACCCGCGTGACTCTGGAAT
CAAATGTCACTCTGCAATACCGCACTTTTACCCTGAGTGGTCCGGACAGGCTGGTGCTAGATATCGAAGAGCTGCGGCTC
AATTCGATGCTCAGCCAGCTAGGGGAGCGGGTGCGGCCGGAAGATCCTTATATCGGCCAGGTACGGGTTGGTCAGTTCGA
CGGCCATACCGTGCGGCTGGTGCTGGAACTGAAACAGAAAGTGGCGCCGCGCTTTTTGACGCTGGCGCCGGCCCACGGCT
TCCGGCACAGGTTGGTGCTGGATCTTTACCCTCATGAGGGCAGTCGTTCCTCGTTGCAAGACGATCCGCTGCTGGCGTTG
CTAGAAGATTATAACAAGGGTGATTTGGCACGTACTTTGCCGCCGGAGTCTCAGGCGCCACTGGCCGGGAAAGCCGGACG
CGACCGGCCGGTTGTGGTGATGCTTGACCCGGGCCACGGCGGCGAAGATCCCGGCGCGATCGGCAAATTCAAAACCCGCG
AGAAAGATATCGTGCTGCAAATCGCCCGCCGTTTAAGAGCGCTGATTAAGCGCGAACCGAATATGCAGGTGTATATGACC
CGCAATGAGGATGTGTTCATCCCGCTGAAGGTGCGGGTGGCCAAGGCACGTAAGCAGCGGGCGGATCTGTTTGTTTCGAT
ACACGCCGACGCCTTTACCCGCCGTAGCGCCCACGGCGCCTCGGTCTTTGCGTTGTCCACCAAGGGAGCCACCAGCGCGG
CGGCCCGCTATCTGGCCCAAACGCAAAATGCTGCCGATGAAATTGGTGGCGTGAGCAAAAGCGGCGATCGCTACCTGGAT
CATACCATTTTCGATTTAATGCAGACCGCTACGATTAACGATAGCCTGAAATTTGGCAAGCACGTGCTGAGCCACGTGAG
TAAGATCACGCAGTTGCATAAAAAAAGCGTCGATCAGGCGGGCTTCGCGGTATTGAAAGCGCCGGATATTCCGTCAATTC
TGGTGGAAACCGCGTTTATCAGCAATGTTCAGGAAGAGCGGCGGTTGCGCACCGCCCGTTACCAGCAGCAGATGGCGGAA
GCCATTTTGGCAGGGATCAAAGCCTATTTTGCCGCCGGTGGAGTATTGGCGCGGCGTTAG

Upstream 100 bases:

>100_bases
CTTTTCGGGATGACAGCGGCCGGCGGTTTGGTTAAAGTTTCCGCCGTTAACGATAAACGATTGGTTTTCCAGATAATGCA
CAATTTATTACCGGGGCGAC

Downstream 100 bases:

>100_bases
CTCGCGGTGCCTGGGAACTCGGCGCCTGGCGTGGCTGATGCTAACATCCGCGCCCGGGGCGGCCTGCACGAGCGCCGTTT
GGCGTTGGCCCACGCCGTCC

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 419; Mature: 418

Protein sequence:

>419_residues
MPESEHNRARRLLLQGVAATWLLSVSRVGLAAAPSIVAVRIWPASSYTRVTLESNVTLQYRTFTLSGPDRLVLDIEELRL
NSMLSQLGERVRPEDPYIGQVRVGQFDGHTVRLVLELKQKVAPRFLTLAPAHGFRHRLVLDLYPHEGSRSSLQDDPLLAL
LEDYNKGDLARTLPPESQAPLAGKAGRDRPVVVMLDPGHGGEDPGAIGKFKTREKDIVLQIARRLRALIKREPNMQVYMT
RNEDVFIPLKVRVAKARKQRADLFVSIHADAFTRRSAHGASVFALSTKGATSAAARYLAQTQNAADEIGGVSKSGDRYLD
HTIFDLMQTATINDSLKFGKHVLSHVSKITQLHKKSVDQAGFAVLKAPDIPSILVETAFISNVQEERRLRTARYQQQMAE
AILAGIKAYFAAGGVLARR

Sequences:

>Translated_419_residues
MPESEHNRARRLLLQGVAATWLLSVSRVGLAAAPSIVAVRIWPASSYTRVTLESNVTLQYRTFTLSGPDRLVLDIEELRL
NSMLSQLGERVRPEDPYIGQVRVGQFDGHTVRLVLELKQKVAPRFLTLAPAHGFRHRLVLDLYPHEGSRSSLQDDPLLAL
LEDYNKGDLARTLPPESQAPLAGKAGRDRPVVVMLDPGHGGEDPGAIGKFKTREKDIVLQIARRLRALIKREPNMQVYMT
RNEDVFIPLKVRVAKARKQRADLFVSIHADAFTRRSAHGASVFALSTKGATSAAARYLAQTQNAADEIGGVSKSGDRYLD
HTIFDLMQTATINDSLKFGKHVLSHVSKITQLHKKSVDQAGFAVLKAPDIPSILVETAFISNVQEERRLRTARYQQQMAE
AILAGIKAYFAAGGVLARR
>Mature_418_residues
PESEHNRARRLLLQGVAATWLLSVSRVGLAAAPSIVAVRIWPASSYTRVTLESNVTLQYRTFTLSGPDRLVLDIEELRLN
SMLSQLGERVRPEDPYIGQVRVGQFDGHTVRLVLELKQKVAPRFLTLAPAHGFRHRLVLDLYPHEGSRSSLQDDPLLALL
EDYNKGDLARTLPPESQAPLAGKAGRDRPVVVMLDPGHGGEDPGAIGKFKTREKDIVLQIARRLRALIKREPNMQVYMTR
NEDVFIPLKVRVAKARKQRADLFVSIHADAFTRRSAHGASVFALSTKGATSAAARYLAQTQNAADEIGGVSKSGDRYLDH
TIFDLMQTATINDSLKFGKHVLSHVSKITQLHKKSVDQAGFAVLKAPDIPSILVETAFISNVQEERRLRTARYQQQMAEA
ILAGIKAYFAAGGVLARR

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI87082163, Length=421, Percent_Identity=68.646080760095, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1788776, Length=246, Percent_Identity=48.3739837398374, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1790611, Length=227, Percent_Identity=42.7312775330396, Blast_Score=186, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR021731
- InterPro:   IPR002508
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 46385; Mature: 46254

Theoretical pI: Translated: 10.55; Mature: 10.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPESEHNRARRLLLQGVAATWLLSVSRVGLAAAPSIVAVRIWPASSYTRVTLESNVTLQY
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEECCEEEEE
RTFTLSGPDRLVLDIEELRLNSMLSQLGERVRPEDPYIGQVRVGQFDGHTVRLVLELKQK
EEEEECCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEHHHHHHHH
VAPRFLTLAPAHGFRHRLVLDLYPHEGSRSSLQDDPLLALLEDYNKGDLARTLPPESQAP
HCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCC
LAGKAGRDRPVVVMLDPGHGGEDPGAIGKFKTREKDIVLQIARRLRALIKREPNMQVYMT
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
RNEDVFIPLKVRVAKARKQRADLFVSIHADAFTRRSAHGASVFALSTKGATSAAARYLAQ
CCCCEEEEEEEHHHHHHHHCCCEEEEEECCHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
TQNAADEIGGVSKSGDRYLDHTIFDLMQTATINDSLKFGKHVLSHVSKITQLHKKSVDQA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
GFAVLKAPDIPSILVETAFISNVQEERRLRTARYQQQMAEAILAGIKAYFAAGGVLARR
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure 
PESEHNRARRLLLQGVAATWLLSVSRVGLAAAPSIVAVRIWPASSYTRVTLESNVTLQY
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEEECCEEEEE
RTFTLSGPDRLVLDIEELRLNSMLSQLGERVRPEDPYIGQVRVGQFDGHTVRLVLELKQK
EEEEECCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEHHHHHHHH
VAPRFLTLAPAHGFRHRLVLDLYPHEGSRSSLQDDPLLALLEDYNKGDLARTLPPESQAP
HCCCEEEECCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCC
LAGKAGRDRPVVVMLDPGHGGEDPGAIGKFKTREKDIVLQIARRLRALIKREPNMQVYMT
CCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
RNEDVFIPLKVRVAKARKQRADLFVSIHADAFTRRSAHGASVFALSTKGATSAAARYLAQ
CCCCEEEEEEEHHHHHHHHCCCEEEEEECCHHHHCCCCCCEEEEEECCCCHHHHHHHHHH
TQNAADEIGGVSKSGDRYLDHTIFDLMQTATINDSLKFGKHVLSHVSKITQLHKKSVDQA
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCHHH
GFAVLKAPDIPSILVETAFISNVQEERRLRTARYQQQMAEAILAGIKAYFAAGGVLARR
CEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]