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The map label for this gene is yfiF [H]

Identifier: 85059886

GI number: 85059886

Start: 3293766

End: 3294869

Strand: Reverse

Name: yfiF [H]

Synonym: SG1908

Alternate gene names: 85059886

Gene position: 3294869-3293766 (Counterclockwise)

Preceding gene: 85059890

Following gene: 85059880

Centisome position: 78.99

GC content: 61.78

Gene sequence:

>1104_bases
ATGAGTGATAACGATTCGTTAAAGGGCAAAAACGGCAAAGTCCGCGTGATGTATGTGCGCAAAGAGGAAGAGCAGGAGCA
AGCGCGCCGCCCGTCCTCGCGCGCCTCCGCTGACGATCGCAGGGGCGACCAGCGCAGTGAGGGCCGTAGCCCGTGGAACA
CCTCCGGCGATCGCGCCTCGGGTGAGGTGTCGCGTCGCGCCCGTCAGGACTCCCCGTTCCGCGACGATAGCACTGGCCGG
CGTCCGCGCGGCGATGCGCCGGATTCGCCGTGGAAAACCTTTTACGCCGGCGATGATGATGCCACCGTGCCAGATCATGG
CGGTATCAGCGGCAAAAGTCAGATTGATCCCGAACAATTACGCCGCCAGCGCGCCGAAGAAACCCGTGTCTACGGTGAAA
ATGCCTGTCAGGCGCTGTTTCGCAACCGGCCGGACGCCATTGTCCGGGCCTGGTTCGAGCAATCGGTGACGCCGAGGTTT
CGCGATGCGCTGCGCTGGATGGCGGCCAATCGTAAAGCTTATCATGTGGTGGAATATGATGAGCTGGCCAAAGCCTCGGG
CACCGAGCACCATGGCGGCGTCTGTTTTCTGATTAAAAAGCGCGCCGGACTTGATGCCGACGGCTATCTGGCCAGCGCCG
CTGCGCAACAACAGGACTGCGTGCTGGCGCTGGAGCAGGTGAGCAATCCCCACAATCTCGGTGCGATGTTGCGCAGCTGT
GCCCATTTCGGCGTAAATAGCGTGATGGTAAGCGACAGCGCCCTGCTGGATTCCGGCGCCGCGGTGCGCACCGCGGAAGG
GGGGGCAGAGCACGTCAAAGCCATTAAATGCGATGATGTGTTGCAGGACCTGCAGCGTTTCCAACGTGCCGGTTACACCG
TGGTCACGCTCGCCGGTAACAGCGGCACGTCGCTGTCGACGGTCAAAATGCCTGCTCGGACGGTGCTGGTGCTGGGAGAA
GAAGGTAGCGCGTTGTCGGAAGCGGTACTGTCGCAGGCGGATATCGCTGTGACCATCAACGGCAGCGGCCGGGTACCGAG
TTTAAATGTGTCCGTCAGCGCCGGTATTCTGCTGGCGGAATGGTGGCGGCAAAACCACGGCTAA

Upstream 100 bases:

>100_bases
TGTTCAATGGTTTCGATAGTTGCCGGCTTTTACCTTAACCCGGTAGCTATATGGATGGAGATGGGGTAATCTTCGCGCCC
ATCAGAGGGGGGAGGGCAAT

Downstream 100 bases:

>100_bases
CGGCGGCGCAACGGCGCAAAAAAAACGCCGCGGGAGAGTCCTCGCGGCGTTTGCTAGGGTAGGCGGTTAAACGCACTTCA
GCACATCTTCTTACGCGTTA

Product: putative methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MSDNDSLKGKNGKVRVMYVRKEEEQEQARRPSSRASADDRRGDQRSEGRSPWNTSGDRASGEVSRRARQDSPFRDDSTGR
RPRGDAPDSPWKTFYAGDDDATVPDHGGISGKSQIDPEQLRRQRAEETRVYGENACQALFRNRPDAIVRAWFEQSVTPRF
RDALRWMAANRKAYHVVEYDELAKASGTEHHGGVCFLIKKRAGLDADGYLASAAAQQQDCVLALEQVSNPHNLGAMLRSC
AHFGVNSVMVSDSALLDSGAAVRTAEGGAEHVKAIKCDDVLQDLQRFQRAGYTVVTLAGNSGTSLSTVKMPARTVLVLGE
EGSALSEAVLSQADIAVTINGSGRVPSLNVSVSAGILLAEWWRQNHG

Sequences:

>Translated_367_residues
MSDNDSLKGKNGKVRVMYVRKEEEQEQARRPSSRASADDRRGDQRSEGRSPWNTSGDRASGEVSRRARQDSPFRDDSTGR
RPRGDAPDSPWKTFYAGDDDATVPDHGGISGKSQIDPEQLRRQRAEETRVYGENACQALFRNRPDAIVRAWFEQSVTPRF
RDALRWMAANRKAYHVVEYDELAKASGTEHHGGVCFLIKKRAGLDADGYLASAAAQQQDCVLALEQVSNPHNLGAMLRSC
AHFGVNSVMVSDSALLDSGAAVRTAEGGAEHVKAIKCDDVLQDLQRFQRAGYTVVTLAGNSGTSLSTVKMPARTVLVLGE
EGSALSEAVLSQADIAVTINGSGRVPSLNVSVSAGILLAEWWRQNHG
>Mature_366_residues
SDNDSLKGKNGKVRVMYVRKEEEQEQARRPSSRASADDRRGDQRSEGRSPWNTSGDRASGEVSRRARQDSPFRDDSTGRR
PRGDAPDSPWKTFYAGDDDATVPDHGGISGKSQIDPEQLRRQRAEETRVYGENACQALFRNRPDAIVRAWFEQSVTPRFR
DALRWMAANRKAYHVVEYDELAKASGTEHHGGVCFLIKKRAGLDADGYLASAAAQQQDCVLALEQVSNPHNLGAMLRSCA
HFGVNSVMVSDSALLDSGAAVRTAEGGAEHVKAIKCDDVLQDLQRFQRAGYTVVTLAGNSGTSLSTVKMPARTVLVLGEE
GSALSEAVLSQADIAVTINGSGRVPSLNVSVSAGILLAEWWRQNHG

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=364, Percent_Identity=56.043956043956, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1790623, Length=238, Percent_Identity=29.4117647058824, Blast_Score=85, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 39998; Mature: 39866

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNDSLKGKNGKVRVMYVRKEEEQEQARRPSSRASADDRRGDQRSEGRSPWNTSGDRAS
CCCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCHHHCCCCHHCCCCCCCCCCCCCC
GEVSRRARQDSPFRDDSTGRRPRGDAPDSPWKTFYAGDDDATVPDHGGISGKSQIDPEQL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHH
RRQRAEETRVYGENACQALFRNRPDAIVRAWFEQSVTPRFRDALRWMAANRKAYHVVEYD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECHH
ELAKASGTEHHGGVCFLIKKRAGLDADGYLASAAAQQQDCVLALEQVSNPHNLGAMLRSC
HHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AHFGVNSVMVSDSALLDSGAAVRTAEGGAEHVKAIKCDDVLQDLQRFQRAGYTVVTLAGN
HHCCCCEEEECCHHHHCCCCEEEECCCCHHHHHEECHHHHHHHHHHHHHCCCEEEEEECC
SGTSLSTVKMPARTVLVLGEEGSALSEAVLSQADIAVTINGSGRVPSLNVSVSAGILLAE
CCCCEEEEECCCEEEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCEEEEEHHCCHHHHH
WWRQNHG
HHHHCCC
>Mature Secondary Structure 
SDNDSLKGKNGKVRVMYVRKEEEQEQARRPSSRASADDRRGDQRSEGRSPWNTSGDRAS
CCCCCCCCCCCCEEEEEECCHHHHHHHHCCCCCCCCHHHCCCCHHCCCCCCCCCCCCCC
GEVSRRARQDSPFRDDSTGRRPRGDAPDSPWKTFYAGDDDATVPDHGGISGKSQIDPEQL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHH
RRQRAEETRVYGENACQALFRNRPDAIVRAWFEQSVTPRFRDALRWMAANRKAYHVVEYD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEECHH
ELAKASGTEHHGGVCFLIKKRAGLDADGYLASAAAQQQDCVLALEQVSNPHNLGAMLRSC
HHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
AHFGVNSVMVSDSALLDSGAAVRTAEGGAEHVKAIKCDDVLQDLQRFQRAGYTVVTLAGN
HHCCCCEEEECCHHHHCCCCEEEECCCCHHHHHEECHHHHHHHHHHHHHCCCEEEEEECC
SGTSLSTVKMPARTVLVLGEEGSALSEAVLSQADIAVTINGSGRVPSLNVSVSAGILLAE
CCCCEEEEECCCEEEEEECCCCCHHHHHHHCCCCEEEEECCCCCCCEEEEEHHCCHHHHH
WWRQNHG
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]