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The map label for this gene is mexA [H]

Identifier: 85059842

GI number: 85059842

Start: 3196148

End: 3196942

Strand: Reverse

Name: mexA [H]

Synonym: SG1864

Alternate gene names: 85059842

Gene position: 3196942-3196148 (Counterclockwise)

Preceding gene: 85059843

Following gene: 85059841

Centisome position: 76.64

GC content: 57.11

Gene sequence:

>795_bases
ATGGTATTAGGCGCGATGTCTCTCTTAAGATTTTTGGCGGTAGGTCTGATATTTTTCGTCGTGGCCTGCGATCGGCAACC
GGAAGCCAGTCCCGACGATGTGGTAACCGAGGTGGGTGTGAAAACGTTGCAGGCTCAGCCGGTTACGTTGATCAGCGACT
TGACCGGTCGGGTAAAAGGTAGGCTGGTTTCTGAGGTTCGCCCCCAAGTGGACGGTATCATTCAAAAGCGGCTATTTACC
GAAGGCGATGAGGTCAAGGAAGGGCAGGCGCTGTATCAAATCGACCTCGTCAGCTACCAGGCCAGCTATGATGAGGCGCT
GGGGCAATTGAAAAATGCCGTCGCGGTGGTGAACAGTAGCAAACTTAAGGCGCAGCGCTATGCGGCGCTGGTTAAGGAAA
ACGTCGTCTCCCACCAGGACGCCGATGAAGTGGCGGCCACATGGCAACAAAATGTCGCCGCGGTGGATCAATATCGTGCC
GCGGTACAAAGCGCGCGTATCGATCTGCAACGAACGCTTATCAAAGCGCCTATTTCCGGCCGCATCGGGATATCGTCGGT
TACCCCCGGCGCTCTGGTAGAAGCCAATCAGTCCGACGCGCTGGCCACGATCCGCGCGCTGAATCCTATCTATGTCGATC
TTACCCAGTCCAGCAGTCAGCTGTTGCAACTGCGCCGCCAGCAGGCGTCGCTGAAGCAGGAAGGCGACACGGTACCGGTG
AAGGTCACGCTGGAAGACGGCTCGCGCTACCCGCATACCGGTAAACTGGAACTGATCGAGGGTGGCGGTGGATGA

Upstream 100 bases:

>100_bases
TAAGAAACATGTCTTCGTAACAGGTATTTTCGTTTTTTTTTACCTATCGGTCCCCTCGGCACGTTAGTGCCCGCAGGTAT
AATGAGAGTGAACATTCATT

Downstream 100 bases:

>100_bases
GGCAACGGGCACCGTCACGCTGCGCTCTATTGTACCCAATCCCCAGCAGGGCATAACCCGCGATGCCAAAGGTAATGCGA
CCGCTCTGGTGGTCGGCAAC

Product: putative multidrug efflux transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MVLGAMSLLRFLAVGLIFFVVACDRQPEASPDDVVTEVGVKTLQAQPVTLISDLTGRVKGRLVSEVRPQVDGIIQKRLFT
EGDEVKEGQALYQIDLVSYQASYDEALGQLKNAVAVVNSSKLKAQRYAALVKENVVSHQDADEVAATWQQNVAAVDQYRA
AVQSARIDLQRTLIKAPISGRIGISSVTPGALVEANQSDALATIRALNPIYVDLTQSSSQLLQLRRQQASLKQEGDTVPV
KVTLEDGSRYPHTGKLELIEGGGG

Sequences:

>Translated_264_residues
MVLGAMSLLRFLAVGLIFFVVACDRQPEASPDDVVTEVGVKTLQAQPVTLISDLTGRVKGRLVSEVRPQVDGIIQKRLFT
EGDEVKEGQALYQIDLVSYQASYDEALGQLKNAVAVVNSSKLKAQRYAALVKENVVSHQDADEVAATWQQNVAAVDQYRA
AVQSARIDLQRTLIKAPISGRIGISSVTPGALVEANQSDALATIRALNPIYVDLTQSSSQLLQLRRQQASLKQEGDTVPV
KVTLEDGSRYPHTGKLELIEGGGG
>Mature_264_residues
MVLGAMSLLRFLAVGLIFFVVACDRQPEASPDDVVTEVGVKTLQAQPVTLISDLTGRVKGRLVSEVRPQVDGIIQKRLFT
EGDEVKEGQALYQIDLVSYQASYDEALGQLKNAVAVVNSSKLKAQRYAALVKENVVSHQDADEVAATWQQNVAAVDQYRA
AVQSARIDLQRTLIKAPISGRIGISSVTPGALVEANQSDALATIRALNPIYVDLTQSSSQLLQLRRQQASLKQEGDTVPV
KVTLEDGSRYPHTGKLELIEGGGG

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=258, Percent_Identity=42.2480620155039, Blast_Score=185, Evalue=2e-48,
Organism=Escherichia coli, GI1786668, Length=241, Percent_Identity=43.5684647302905, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789665, Length=242, Percent_Identity=41.3223140495868, Blast_Score=174, Evalue=4e-45,
Organism=Escherichia coli, GI87082036, Length=150, Percent_Identity=30.6666666666667, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 28480; Mature: 28480

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLGAMSLLRFLAVGLIFFVVACDRQPEASPDDVVTEVGVKTLQAQPVTLISDLTGRVKG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RLVSEVRPQVDGIIQKRLFTEGDEVKEGQALYQIDLVSYQASYDEALGQLKNAVAVVNSS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCH
KLKAQRYAALVKENVVSHQDADEVAATWQQNVAAVDQYRAAVQSARIDLQRTLIKAPISG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RIGISSVTPGALVEANQSDALATIRALNPIYVDLTQSSSQLLQLRRQQASLKQEGDTVPV
CCCCCCCCCCCEEECCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEE
KVTLEDGSRYPHTGKLELIEGGGG
EEEEECCCCCCCCCCEEEEECCCC
>Mature Secondary Structure
MVLGAMSLLRFLAVGLIFFVVACDRQPEASPDDVVTEVGVKTLQAQPVTLISDLTGRVKG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
RLVSEVRPQVDGIIQKRLFTEGDEVKEGQALYQIDLVSYQASYDEALGQLKNAVAVVNSS
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHCCH
KLKAQRYAALVKENVVSHQDADEVAATWQQNVAAVDQYRAAVQSARIDLQRTLIKAPISG
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RIGISSVTPGALVEANQSDALATIRALNPIYVDLTQSSSQLLQLRRQQASLKQEGDTVPV
CCCCCCCCCCCEEECCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEE
KVTLEDGSRYPHTGKLELIEGGGG
EEEEECCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]