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The map label for this gene is grpE

Identifier: 85059776

GI number: 85059776

Start: 3066409

End: 3066996

Strand: Reverse

Name: grpE

Synonym: SG1798

Alternate gene names: 85059776

Gene position: 3066996-3066409 (Counterclockwise)

Preceding gene: 85059780

Following gene: 85059774

Centisome position: 73.53

GC content: 55.61

Gene sequence:

>588_bases
ATGAGTAGTAAAGAACAGAACACCCCTGACGAGCAAGTCTCACAAGAGAGTGAAATGGAGCAAGGGCAGCAAGCGGAAGC
GGCGCCTGAGACGGTTGATGTTGTGGATCCGCGAGATGAGCGTATTGCTGAATTGGAAGCGGCGTTAAGCCAGGCGCAAC
AGCGTGAGCACGATAGCGTGCTGCGCGCCAAGGCGGAAATGGAAAACGTGCGTCGCCGCAGTGAACAGGATGTCGAGAAG
GCGCATAAATTTGCGCTGGAACGTTTCGCCGGTGAACTGTTGCCGGTTATCGATAATTTAGAGCGCGCGCTGGATATGTC
GGATAAAGCTAACGCCGAGCTGGCGTCCACGATTGAGGGAATCGAGCTGACGCTCAAATCGTTGCTGGACGCGGTACGCA
AATTCGGCCTGGATGTGGTGGGAGACACGCACGTGCCGTTTAACCCCGAGGTGCATCAGGCGATGACGATGCTGGAGTCC
GACGAGCATGAGCCCAACCAAGTGATGATGGTGATGCAAAAAGGCTACACCCTCAATGGCCGGCTTATCCGCCCGGCAAT
GGTTGCGGTATCGAAAGCCAAAAGCTGA

Upstream 100 bases:

>100_bases
TTTGCAAGCTTCATTATGCGTCATCCCTTGAATCCCCGCTATTCATCCCCATAATAACCGGACGAGCGAGAATAATGCTG
AACCGCGGAGAAAACTCATG

Downstream 100 bases:

>100_bases
CGTTTTTCCCTCCTGCCGCCCGCGGCGGGAGGGGCCACCCCGATAGCCGCCGCCGTTGATAAAAGCATAGACCGGCGGCC
AGCGACCGCCCTTAACGGCC

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 195; Mature: 194

Protein sequence:

>195_residues
MSSKEQNTPDEQVSQESEMEQGQQAEAAPETVDVVDPRDERIAELEAALSQAQQREHDSVLRAKAEMENVRRRSEQDVEK
AHKFALERFAGELLPVIDNLERALDMSDKANAELASTIEGIELTLKSLLDAVRKFGLDVVGDTHVPFNPEVHQAMTMLES
DEHEPNQVMMVMQKGYTLNGRLIRPAMVAVSKAKS

Sequences:

>Translated_195_residues
MSSKEQNTPDEQVSQESEMEQGQQAEAAPETVDVVDPRDERIAELEAALSQAQQREHDSVLRAKAEMENVRRRSEQDVEK
AHKFALERFAGELLPVIDNLERALDMSDKANAELASTIEGIELTLKSLLDAVRKFGLDVVGDTHVPFNPEVHQAMTMLES
DEHEPNQVMMVMQKGYTLNGRLIRPAMVAVSKAKS
>Mature_194_residues
SSKEQNTPDEQVSQESEMEQGQQAEAAPETVDVVDPRDERIAELEAALSQAQQREHDSVLRAKAEMENVRRRSEQDVEKA
HKFALERFAGELLPVIDNLERALDMSDKANAELASTIEGIELTLKSLLDAVRKFGLDVVGDTHVPFNPEVHQAMTMLESD
EHEPNQVMMVMQKGYTLNGRLIRPAMVAVSKAKS

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=136, Percent_Identity=34.5588235294118, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1788967, Length=197, Percent_Identity=60.9137055837564, Blast_Score=246, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI17552458, Length=141, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6324806, Length=160, Percent_Identity=33.75, Blast_Score=92, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24653432, Length=175, Percent_Identity=32.5714285714286, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_SODGM (Q2NS02)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455478.1
- ProteinModelPortal:   Q2NS02
- SMR:   Q2NS02
- STRING:   Q2NS02
- GeneID:   3866801
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1798
- NMPDR:   fig|343509.6.peg.3824
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NLHQAVM
- PhylomeDB:   Q2NS02
- ProtClustDB:   PRK14150
- BioCyc:   SGLO343509:SG1798-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21796; Mature: 21665

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKEQNTPDEQVSQESEMEQGQQAEAAPETVDVVDPRDERIAELEAALSQAQQREHDSV
CCCCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRAKAEMENVRRRSEQDVEKAHKFALERFAGELLPVIDNLERALDMSDKANAELASTIEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
IELTLKSLLDAVRKFGLDVVGDTHVPFNPEVHQAMTMLESDEHEPNQVMMVMQKGYTLNG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
RLIRPAMVAVSKAKS
CCHHHHHHHHHHCCC
>Mature Secondary Structure 
SSKEQNTPDEQVSQESEMEQGQQAEAAPETVDVVDPRDERIAELEAALSQAQQREHDSV
CCCCCCCCHHHHHHHHHHHHCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LRAKAEMENVRRRSEQDVEKAHKFALERFAGELLPVIDNLERALDMSDKANAELASTIEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
IELTLKSLLDAVRKFGLDVVGDTHVPFNPEVHQAMTMLESDEHEPNQVMMVMQKGYTLNG
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
RLIRPAMVAVSKAKS
CCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA