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The map label for this gene is ispG

Identifier: 85059738

GI number: 85059738

Start: 3015352

End: 3016473

Strand: Reverse

Name: ispG

Synonym: SG1760

Alternate gene names: 85059738

Gene position: 3016473-3015352 (Counterclockwise)

Preceding gene: 85059739

Following gene: 85059737

Centisome position: 72.32

GC content: 56.68

Gene sequence:

>1122_bases
ATGCATAATTCCGCACCCATAACCCGTCGAAAATCGAAACGTATTTACGTCGGCAAGGTGCTGGTGGGTGACGGCGCCCC
CATCGCGGTACAGTCAATGACCAACACCTGTACCACCGATGTCGCGGCGACGGTGCAACAGATACAGGCGCTGGAGCGCG
TCGGTGTTGATATCGTACGCGTATCGGTGCCCACCATGGACGCCGCCGAAGCGTTCAAACTTATCAAACAGCAGGTCGCT
GTTCCGCTGGTGGCGGATATCCATTTCGATTATCGCATCGCGTTGAAAGTTGCCGAATATGGCGTTGACTGTTTGCGTAT
CAATCCCGGCAACATCGGCAATGAGGGGCGTATCCGTTCCGTGGTGGATTGCGCGCGCGATAACAATATCCCGATTCGCA
TCGGCGTTAACGCTGGCTCCCTTGAGCGCGATCTGCAGGAAAAGTATGGCGAACCCACGCCGGAGGCCCTGTTGGAATCG
GCCATGCGCCATGTGGATATCCTTGACCGGCTCAATTTCGATACGTTCAAGGTCAGCGTGAAAGCTTCCGACGTGTTCCT
GGCGGTGCAATCCTACCGCCTTTTGGCCTCGCGTATCGATCAGCCGCTGCATCTGGGTATTACCGAGGCCGGCGGCGCCC
GCAGCGGGGCGGTGAAATCGGCGATTGGTCTTGGCCTGCTATTAAGCGAAGGCATCGGCGATACGCTGCGCATCTCGCTG
GCGGCGGATCCGGTAGAGGAAGTAAAGGTCGGTTTCGATATTTTGAAGTCGCTGCGTATTCGCGCCCGCGGCATCAACTT
CATTGCCTGCCCGACGTGTTCGCGTCAGGAGTTTGATGTTATCGGCACCGTCAATGCCCTGGAGCAGCGGCTGGAGGACA
TCGTCACGCCAATGGATGTCTCCATCATTGGTTGTGTAGTGAATGGTCCCGGCGAAGCGCTGGTGTCCACGCTGGGTGTG
ACCGGCGGCCATAACAAAAGCGGCTACTATGAAGACGGCGTTCGCCAGCGCGAGCGTTTCGATAACGAACTGATGATCGA
CCAATTAGAGGCCCGTATTCGCGCGAAAGCGGCGATGCTGGACGAGAATAATCGCATCACGGTCAATCTGATAGAAAAAT
AA

Upstream 100 bases:

>100_bases
GGTGGATCTGAGCCGGTTCATCCGGTCAAATCAGGTTGCGCGACTGACCGTAGCCGCCCAGTAAAGAGTTAAGCCAGTCG
GCAAGGTTGGAGAAAGTTCA

Downstream 100 bases:

>100_bases
CACAGGTAGAGATTCGACATGGCAAAGAACATCCAGGCCATTCGCGGCATGAACGACTATTTGCCGGAAGAGACGGCATT
CTGGCAGTGCGTGGAAGGGA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MHNSAPITRRKSKRIYVGKVLVGDGAPIAVQSMTNTCTTDVAATVQQIQALERVGVDIVRVSVPTMDAAEAFKLIKQQVA
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEGRIRSVVDCARDNNIPIRIGVNAGSLERDLQEKYGEPTPEALLES
AMRHVDILDRLNFDTFKVSVKASDVFLAVQSYRLLASRIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRISL
AADPVEEVKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDIVTPMDVSIIGCVVNGPGEALVSTLGV
TGGHNKSGYYEDGVRQRERFDNELMIDQLEARIRAKAAMLDENNRITVNLIEK

Sequences:

>Translated_373_residues
MHNSAPITRRKSKRIYVGKVLVGDGAPIAVQSMTNTCTTDVAATVQQIQALERVGVDIVRVSVPTMDAAEAFKLIKQQVA
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEGRIRSVVDCARDNNIPIRIGVNAGSLERDLQEKYGEPTPEALLES
AMRHVDILDRLNFDTFKVSVKASDVFLAVQSYRLLASRIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRISL
AADPVEEVKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDIVTPMDVSIIGCVVNGPGEALVSTLGV
TGGHNKSGYYEDGVRQRERFDNELMIDQLEARIRAKAAMLDENNRITVNLIEK
>Mature_373_residues
MHNSAPITRRKSKRIYVGKVLVGDGAPIAVQSMTNTCTTDVAATVQQIQALERVGVDIVRVSVPTMDAAEAFKLIKQQVA
VPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEGRIRSVVDCARDNNIPIRIGVNAGSLERDLQEKYGEPTPEALLES
AMRHVDILDRLNFDTFKVSVKASDVFLAVQSYRLLASRIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRISL
AADPVEEVKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDIVTPMDVSIIGCVVNGPGEALVSTLGV
TGGHNKSGYYEDGVRQRERFDNELMIDQLEARIRAKAAMLDENNRITVNLIEK

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=373, Percent_Identity=88.4718498659517, Blast_Score=662, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 40586; Mature: 40586

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHNSAPITRRKSKRIYVGKVLVGDGAPIAVQSMTNTCTTDVAATVQQIQALERVGVDIVR
CCCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEE
VSVPTMDAAEAFKLIKQQVAVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEGRIRS
EECCCCCHHHHHHHHHHHHCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCCCCHHH
VVDCARDNNIPIRIGVNAGSLERDLQEKYGEPTPEALLESAMRHVDILDRLNFDTFKVSV
HHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
KASDVFLAVQSYRLLASRIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRISL
EECCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEE
AADPVEEVKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDIVTPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEECHHHHHHHHHHHCCCCCE
SIIGCVVNGPGEALVSTLGVTGGHNKSGYYEDGVRQRERFDNELMIDQLEARIRAKAAML
EEEEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
DENNRITVNLIEK
CCCCEEEEEEECC
>Mature Secondary Structure
MHNSAPITRRKSKRIYVGKVLVGDGAPIAVQSMTNTCTTDVAATVQQIQALERVGVDIVR
CCCCCCCCCCCCCEEEEEEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCEEEE
VSVPTMDAAEAFKLIKQQVAVPLVADIHFDYRIALKVAEYGVDCLRINPGNIGNEGRIRS
EECCCCCHHHHHHHHHHHHCCCEEEEECCCEEEEEEEHHCCCEEEEECCCCCCCCCCHHH
VVDCARDNNIPIRIGVNAGSLERDLQEKYGEPTPEALLESAMRHVDILDRLNFDTFKVSV
HHHHHCCCCCCEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEE
KASDVFLAVQSYRLLASRIDQPLHLGITEAGGARSGAVKSAIGLGLLLSEGIGDTLRISL
EECCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEE
AADPVEEVKVGFDILKSLRIRARGINFIACPTCSRQEFDVIGTVNALEQRLEDIVTPMDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCEECHHHHHHHHHHHCCCCCE
SIIGCVVNGPGEALVSTLGVTGGHNKSGYYEDGVRQRERFDNELMIDQLEARIRAKAAML
EEEEEEECCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHC
DENNRITVNLIEK
CCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA