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The map label for this gene is hisS

Identifier: 85059737

GI number: 85059737

Start: 3014059

End: 3015333

Strand: Reverse

Name: hisS

Synonym: SG1759

Alternate gene names: 85059737

Gene position: 3015333-3014059 (Counterclockwise)

Preceding gene: 85059738

Following gene: 85059736

Centisome position: 72.29

GC content: 60.86

Gene sequence:

>1275_bases
ATGGCAAAGAACATCCAGGCCATTCGCGGCATGAACGACTATTTGCCGGAAGAGACGGCATTCTGGCAGTGCGTGGAAGG
GACACTTAAAAACGTGCTGGCCAGCTACGGCTACAGTGAAATCCGCCTGCCGATCGTCGAGCAGACCCCGCTGTTCCAGC
GGGCTATCGGTGAAGTTACCGATGTAGTGGAAAAAGAGATGTATACCTTCGCCGATCGCAACGGCGACAGCCTGACCCTG
CGTCCGGAGGGCACCGCCGGCTGCGTGCGCGCCGGTATTGAACACGGTTTGCTGTACAATCAGGAACAGCGGTTGTGGTA
TGTGGGGCCGATGTTTCGCTATGAGCGTCCACAGAAAGGCCGCTACCGTCAGTTCCACCAGATGGGTGCCGAGGTATTTG
GTCAACAGGGACCGGATGTTGACGCCGAGCTTATTCTGCTGACCGCCCGCTGGTGGCGTGCGCTGGGTATCCAGGAACAT
GTGGCGCTGGAGCTGAATTCCATCGGTTCGCTGGAGGCGTGCGCGCGCTACCGCGAGGCGCTGGTCGCGTTTTTGCGCCA
GCATGAAGACCGCCTGGATGAAGATTGCCGCCGTCGCATGCATACCAATCCAATGCGGGTATTGGACACGAAAAACCCCG
ATATTCAGGTGCTGCTCAACGACGCACCGGTTCTGACCGATTACCTTGATGACGATTCCCAGGCCCACTTTTCCGGTCTG
TGTGAACTTTTAGACCTGGCCGGCATCCCATATACGGTTAATCCGCGTTTGGTGCGCGGCCTGGATTATTACAACCGGAC
GGTTTTCGAGTGGGTAACCACCCGTTTGGGGTCGCAGGGCACCGTTTGCGGCGGCGGCCGCTACGATGGGCTGGTGGAAC
AGCTGGGCGGTCGCGCCACCCCCGCCGTGGGTTTCGCGATGGGGCTGGAGCGGCTGGTGCTGCTGGTGCAGGCGGTCAAC
CCGGATTTCGCCGCCGCAGCGCGTGTGGATGCTTATCTGGTGGCGGCCGGGGACGGCGTGCAGCGTGAGGCGCTGCGGCT
GGCGGAGCAGTTGCGTGACGAGCTGTCTTCCCTGCGGCTGATGACCAATTACGGCGGCGGCAGCTTTAAAAAGCAGTTTG
GCCGTGCCGACAAGCACGGCGCCCGCATCGCGCTGGTTCTCGGAGAAAGCGAAGCGGCGGCGGGACAAGTAGTGGTGAAG
GATTTGGCCACGGGTAACCAGGAAACGCTGGCGCAGAGCGATGTCGCCGCGCGGCTGGCATCGATTTTAGGTTAA

Upstream 100 bases:

>100_bases
CCAATTAGAGGCCCGTATTCGCGCGAAAGCGGCGATGCTGGACGAGAATAATCGCATCACGGTCAATCTGATAGAAAAAT
AACACAGGTAGAGATTCGAC

Downstream 100 bases:

>100_bases
GGAGAGGTACACCGTGGAAGTCTACAGCACTGATAACGAACAGCGCGATGCGCTGCGCCGTTTCTTTGTCGATAACGGCA
AGGCGCTGGCGATAGGCGTC

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAKNIQAIRGMNDYLPEETAFWQCVEGTLKNVLASYGYSEIRLPIVEQTPLFQRAIGEVTDVVEKEMYTFADRNGDSLTL
RPEGTAGCVRAGIEHGLLYNQEQRLWYVGPMFRYERPQKGRYRQFHQMGAEVFGQQGPDVDAELILLTARWWRALGIQEH
VALELNSIGSLEACARYREALVAFLRQHEDRLDEDCRRRMHTNPMRVLDTKNPDIQVLLNDAPVLTDYLDDDSQAHFSGL
CELLDLAGIPYTVNPRLVRGLDYYNRTVFEWVTTRLGSQGTVCGGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVN
PDFAAAARVDAYLVAAGDGVQREALRLAEQLRDELSSLRLMTNYGGGSFKKQFGRADKHGARIALVLGESEAAAGQVVVK
DLATGNQETLAQSDVAARLASILG

Sequences:

>Translated_424_residues
MAKNIQAIRGMNDYLPEETAFWQCVEGTLKNVLASYGYSEIRLPIVEQTPLFQRAIGEVTDVVEKEMYTFADRNGDSLTL
RPEGTAGCVRAGIEHGLLYNQEQRLWYVGPMFRYERPQKGRYRQFHQMGAEVFGQQGPDVDAELILLTARWWRALGIQEH
VALELNSIGSLEACARYREALVAFLRQHEDRLDEDCRRRMHTNPMRVLDTKNPDIQVLLNDAPVLTDYLDDDSQAHFSGL
CELLDLAGIPYTVNPRLVRGLDYYNRTVFEWVTTRLGSQGTVCGGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVN
PDFAAAARVDAYLVAAGDGVQREALRLAEQLRDELSSLRLMTNYGGGSFKKQFGRADKHGARIALVLGESEAAAGQVVVK
DLATGNQETLAQSDVAARLASILG
>Mature_423_residues
AKNIQAIRGMNDYLPEETAFWQCVEGTLKNVLASYGYSEIRLPIVEQTPLFQRAIGEVTDVVEKEMYTFADRNGDSLTLR
PEGTAGCVRAGIEHGLLYNQEQRLWYVGPMFRYERPQKGRYRQFHQMGAEVFGQQGPDVDAELILLTARWWRALGIQEHV
ALELNSIGSLEACARYREALVAFLRQHEDRLDEDCRRRMHTNPMRVLDTKNPDIQVLLNDAPVLTDYLDDDSQAHFSGLC
ELLDLAGIPYTVNPRLVRGLDYYNRTVFEWVTTRLGSQGTVCGGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNP
DFAAAARVDAYLVAAGDGVQREALRLAEQLRDELSSLRLMTNYGGGSFKKQFGRADKHGARIALVLGESEAAAGQVVVKD
LATGNQETLAQSDVAARLASILG

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1788861, Length=424, Percent_Identity=79.7169811320755, Blast_Score=706, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71993686, Length=462, Percent_Identity=24.4588744588745, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71993693, Length=462, Percent_Identity=24.4588744588745, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24643061, Length=477, Percent_Identity=24.3186582809224, Blast_Score=93, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24643059, Length=492, Percent_Identity=24.390243902439, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_SODGM (Q2NS41)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455439.1
- HSSP:   Q02147
- ProteinModelPortal:   Q2NS41
- SMR:   Q2NS41
- STRING:   Q2NS41
- GeneID:   3866918
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1759
- NMPDR:   fig|343509.6.peg.3763
- eggNOG:   COG0124
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- PhylomeDB:   Q2NS41
- ProtClustDB:   PRK00037
- BioCyc:   SGLO343509:SG1759-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47072; Mature: 46941

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNIQAIRGMNDYLPEETAFWQCVEGTLKNVLASYGYSEIRLPIVEQTPLFQRAIGEVT
CCCCHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
DVVEKEMYTFADRNGDSLTLRPEGTAGCVRAGIEHGLLYNQEQRLWYVGPMFRYERPQKG
HHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQMGAEVFGQQGPDVDAELILLTARWWRALGIQEHVALELNSIGSLEACARYREA
HHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHCCHHHHEEECCCCCCHHHHHHHHHH
LVAFLRQHEDRLDEDCRRRMHTNPMRVLDTKNPDIQVLLNDAPVLTDYLDDDSQAHFSGL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHHHHHCCCCCHHHHHHH
CELLDLAGIPYTVNPRLVRGLDYYNRTVFEWVTTRLGSQGTVCGGGRYDGLVEQLGGRAT
HHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCC
PAVGFAMGLERLVLLVQAVNPDFAAAARVDAYLVAAGDGVQREALRLAEQLRDELSSLRL
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHEE
MTNYGGGSFKKQFGRADKHGARIALVLGESEAAAGQVVVKDLATGNQETLAQSDVAARLA
HHCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
SILG
HHHC
>Mature Secondary Structure 
AKNIQAIRGMNDYLPEETAFWQCVEGTLKNVLASYGYSEIRLPIVEQTPLFQRAIGEVT
CCCHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHH
DVVEKEMYTFADRNGDSLTLRPEGTAGCVRAGIEHGLLYNQEQRLWYVGPMFRYERPQKG
HHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCEECCCCCEEEECCHHHCCCCCCC
RYRQFHQMGAEVFGQQGPDVDAELILLTARWWRALGIQEHVALELNSIGSLEACARYREA
HHHHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHCCHHHHEEECCCCCCHHHHHHHHHH
LVAFLRQHEDRLDEDCRRRMHTNPMRVLDTKNPDIQVLLNDAPVLTDYLDDDSQAHFSGL
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEECCCCHHHHHCCCCCHHHHHHH
CELLDLAGIPYTVNPRLVRGLDYYNRTVFEWVTTRLGSQGTVCGGGRYDGLVEQLGGRAT
HHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHCCCCC
PAVGFAMGLERLVLLVQAVNPDFAAAARVDAYLVAAGDGVQREALRLAEQLRDELSSLRL
CHHHHHHHHHHHHHHHHHCCCCHHHHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHEE
MTNYGGGSFKKQFGRADKHGARIALVLGESEAAAGQVVVKDLATGNQETLAQSDVAARLA
HHCCCCCHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHH
SILG
HHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA