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The map label for this gene is thiM [H]

Identifier: 85059717

GI number: 85059717

Start: 2969238

End: 2969816

Strand: Reverse

Name: thiM [H]

Synonym: SG1739

Alternate gene names: 85059717

Gene position: 2969816-2969238 (Counterclockwise)

Preceding gene: 85059718

Following gene: 85059713

Centisome position: 71.2

GC content: 61.31

Gene sequence:

>579_bases
ATGCAACCCTCCGAACTGCCGGGCGCGATTGCCGCCTATACCTTACAGCAGCTGCGCCATCAGGCGCAGCTGGTCCACTG
TTTCACCAATGACGTAGTGCAAACCCTCACCGCCAACATACTGCTGGAGTTGGGCGCCTCCCCTCCCCCGATGGTCGTGG
AGCCGGAAGAGGCCGCGCAATTCAGCGCTATCGCCGATGCCCTATTGATTAATATAGGTACGCTGTGCCAGCAGCGGGTG
GCCGCCATGCTGGCCGGTATTGCTCCTGCCGGTCGCTGCGTTGATAGCGGCGATGATTCCGTGCAGGCGCTGCCCGCCGC
TCGGGTCTTGGCTAAAGCGTGCGGCGCAATAGTGGCGGTGACCGGCGCGGTGGATCTGATTACCGACGGTAACCGTACCT
GGCAGGTAGACGATGGTCATCCGTTAATGACGCGTGTGACTGGTACTGGTTGTGTCCTGTCGGCGGTGGCGGCGGCCTGC
TGCGCCTTGCCCGGTGAGCGGCTGGATAACGTTGCCGGCGCTTTTTCCATTGAGAATAAGGAGCGACGCCGACTGTTTTG
GCACCTGTGTTTCCATTGA

Upstream 100 bases:

>100_bases
ACCCGTATTACCTGAACTGGATCATGCTAGCGTAGGGAAGTCACAGTATCCATTCACCCGCGGAACCGCCTTCTTGATCG
CCGGTGGGAGAATGAAAGCC

Downstream 100 bases:

>100_bases
GGCTATTTCCTTGCTGAATCGAATAGTTTTCCCCCCACGGAGGCTGCATTTATTAATCATAGATACTATCTTGGACGACG
GAAAAGTCGAGCGTCGGCGA

Product: putative hydoxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MQPSELPGAIAAYTLQQLRHQAQLVHCFTNDVVQTLTANILLELGASPPPMVVEPEEAAQFSAIADALLINIGTLCQQRV
AAMLAGIAPAGRCVDSGDDSVQALPAARVLAKACGAIVAVTGAVDLITDGNRTWQVDDGHPLMTRVTGTGCVLSAVAAAC
CALPGERLDNVAGAFSIENKERRRLFWHLCFH

Sequences:

>Translated_192_residues
MQPSELPGAIAAYTLQQLRHQAQLVHCFTNDVVQTLTANILLELGASPPPMVVEPEEAAQFSAIADALLINIGTLCQQRV
AAMLAGIAPAGRCVDSGDDSVQALPAARVLAKACGAIVAVTGAVDLITDGNRTWQVDDGHPLMTRVTGTGCVLSAVAAAC
CALPGERLDNVAGAFSIENKERRRLFWHLCFH
>Mature_192_residues
MQPSELPGAIAAYTLQQLRHQAQLVHCFTNDVVQTLTANILLELGASPPPMVVEPEEAAQFSAIADALLINIGTLCQQRV
AAMLAGIAPAGRCVDSGDDSVQALPAARVLAKACGAIVAVTGAVDLITDGNRTWQVDDGHPLMTRVTGTGCVLSAVAAAC
CALPGERLDNVAGAFSIENKERRRLFWHLCFH

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family [H]

Homologues:

Organism=Escherichia coli, GI1788421, Length=225, Percent_Identity=46.6666666666667, Blast_Score=161, Evalue=2e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000417 [H]

Pfam domain/function: PF02110 HK [H]

EC number: =2.7.1.50 [H]

Molecular weight: Translated: 20196; Mature: 20196

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
4.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPSELPGAIAAYTLQQLRHQAQLVHCFTNDVVQTLTANILLELGASPPPMVVEPEEAAQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHH
FSAIADALLINIGTLCQQRVAAMLAGIAPAGRCVDSGDDSVQALPAARVLAKACGAIVAV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TGAVDLITDGNRTWQVDDGHPLMTRVTGTGCVLSAVAAACCALPGERLDNVAGAFSIENK
CCCEEEEECCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCH
ERRRLFWHLCFH
HHHHHHHHHHCC
>Mature Secondary Structure
MQPSELPGAIAAYTLQQLRHQAQLVHCFTNDVVQTLTANILLELGASPPPMVVEPEEAAQ
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHHHH
FSAIADALLINIGTLCQQRVAAMLAGIAPAGRCVDSGDDSVQALPAARVLAKACGAIVAV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
TGAVDLITDGNRTWQVDDGHPLMTRVTGTGCVLSAVAAACCALPGERLDNVAGAFSIENK
CCCEEEEECCCEEEEECCCCCEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCH
ERRRLFWHLCFH
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA