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The map label for this gene is arrD [H]

Identifier: 85059622

GI number: 85059622

Start: 2808414

End: 2808863

Strand: Reverse

Name: arrD [H]

Synonym: SG1644

Alternate gene names: 85059622

Gene position: 2808863-2808414 (Counterclockwise)

Preceding gene: 85059623

Following gene: 85059621

Centisome position: 67.34

GC content: 54.22

Gene sequence:

>450_bases
ATGAATCCCGAGCTGCGTAACCGATTGATAAAAGGCACAGCAGGCGGTGTTATTGCATTAGCGACCGTGCTAATTCAATG
GCACGAGAGCGTACACTACACTCCCTATCGGGATTCTGGCGGCGTATTGTCGGTCTGTTATGGACATACCGGTAGCGATA
TTGTCCCCGGTAAGCGCTATACCGTTGCTGAATGTCAAGCCCTGCTAGATAGCGACCTGAAAGCCGCAATGTCCGTCGTT
GACGCCAATGTTACCGTACCACTGACTGAGAGCCAAAGAGCGGCGTTAGCCTCGTTCGTCTACAACGTGGGGAACGGGGC
GTTTGCGCGCTCTACGCTACTGAAAAAGCTGAACGCCGGAGACATGGCCGGTGCCTGTGATGAGATGCGGCGCTGGAAAT
ATGTTGGCGGCAAAGTATCGAAGGGACTGGTCAATCGGCGTTACGCCTGA

Upstream 100 bases:

>100_bases
AATGGGCATTGATTACCGGTATCGCATGCACCTTGGCCACCTTTTTGCTGAACTGGATTTACCGGCATAAGGAATACCGT
TTCAGGACGAAACGCGACCC

Downstream 100 bases:

>100_bases
GCGCTGTGCTCGTCTGCCTGTACCTACCAGTGTTGTTAACCGGCTGCGGGCGCACGCAGACCAGATACCTGCCCATCCCG
CCCGCGCTGATACCGGCCAC

Product: phage lysozyme lysis protein

Products: NA

Alternate protein names: Endolysin; Lysis protein; Muramidase [H]

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MNPELRNRLIKGTAGGVIALATVLIQWHESVHYTPYRDSGGVLSVCYGHTGSDIVPGKRYTVAECQALLDSDLKAAMSVV
DANVTVPLTESQRAALASFVYNVGNGAFARSTLLKKLNAGDMAGACDEMRRWKYVGGKVSKGLVNRRYA

Sequences:

>Translated_149_residues
MNPELRNRLIKGTAGGVIALATVLIQWHESVHYTPYRDSGGVLSVCYGHTGSDIVPGKRYTVAECQALLDSDLKAAMSVV
DANVTVPLTESQRAALASFVYNVGNGAFARSTLLKKLNAGDMAGACDEMRRWKYVGGKVSKGLVNRRYA
>Mature_149_residues
MNPELRNRLIKGTAGGVIALATVLIQWHESVHYTPYRDSGGVLSVCYGHTGSDIVPGKRYTVAECQALLDSDLKAAMSVV
DANVTVPLTESQRAALASFVYNVGNGAFARSTLLKKLNAGDMAGACDEMRRWKYVGGKVSKGLVNRRYA

Specific function: Essential for lysis of bacterial cell wall, by showing cell wall hydrolyzing activity [H]

COG id: COG3772

COG function: function code R; Phage-related lysozyme (muraminidase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 24 family [H]

Homologues:

Organism=Escherichia coli, GI1786768, Length=119, Percent_Identity=55.4621848739496, Blast_Score=140, Evalue=2e-35,
Organism=Escherichia coli, GI1787836, Length=125, Percent_Identity=36.8, Blast_Score=86, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002196 [H]

Pfam domain/function: PF00959 Phage_lysozyme [H]

EC number: =3.2.1.17 [H]

Molecular weight: Translated: 16050; Mature: 16050

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPELRNRLIKGTAGGVIALATVLIQWHESVHYTPYRDSGGVLSVCYGHTGSDIVPGKRY
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
TVAECQALLDSDLKAAMSVVDANVTVPLTESQRAALASFVYNVGNGAFARSTLLKKLNAG
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
DMAGACDEMRRWKYVGGKVSKGLVNRRYA
CCCHHHHHHHHHHHCCCHHHHHHHHCCCC
>Mature Secondary Structure
MNPELRNRLIKGTAGGVIALATVLIQWHESVHYTPYRDSGGVLSVCYGHTGSDIVPGKRY
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCC
TVAECQALLDSDLKAAMSVVDANVTVPLTESQRAALASFVYNVGNGAFARSTLLKKLNAG
HHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCC
DMAGACDEMRRWKYVGGKVSKGLVNRRYA
CCCHHHHHHHHHHHCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503 [H]