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The map label for this gene is rnfA

Identifier: 85059433

GI number: 85059433

Start: 2429247

End: 2429828

Strand: Reverse

Name: rnfA

Synonym: SG1455

Alternate gene names: 85059433

Gene position: 2429828-2429247 (Counterclockwise)

Preceding gene: 85059434

Following gene: 85059432

Centisome position: 58.25

GC content: 52.41

Gene sequence:

>582_bases
ATGAATGGTTACCTGATGCTGTTCATCGGCACCGTGCTGGTGAATAACTTTGTGCTGGTCAAATTCCTCGGGCTTTGCCC
GTTTATGGGTGTCTCCAAGAAAGTGGAGACCGCTCTGGGGATGGGCATGGCCACGACATTTGTCATGACGCTGGCGTCGA
TTTGCGCCTGGCTTATTAATGATTTTATTCTGCTGCCGCTCAATATTCCCTATCTGAGAACGCTGGCGTTTATTTTTATT
ATCGCGGTGGTGGTGCAGTTTACCGAACTGGTGGTGCGTAAAACCAGCCCGGCCCTCTATCGTCTGCTCGGCATTTTTCT
GCCGCTGATTACTACTAACTGCGCTGTTCTGGGCGTAGCGCTGCTTAATGTGAATGAAAATCACAATTTTCTTCAGTCCG
CGTTATACGGCTTCAGTGCCGCCGTTGGTTTCTCGCTGGTGATGGTGTTGTTCGCCGCGATTCGCGAACGGCTGGCGGTG
GCGGATGTTCCCGCGCCCTTTAAGGGCTCCTCTATCGCTCTCATTACCGCCGGGTTAATGTCGCTGGCCTTTATGGGCTT
TACCGGACTGGTGAAGGTCTGA

Upstream 100 bases:

>100_bases
CCCCTTGGGTAGGACGGAATTCGCGCACGCATGCGCGAATGGGTTGTCGAACACTCGCAGATCCCGGATAATGCCCGTTG
TTTCTTGTTTAGGTATGGCA

Downstream 100 bases:

>100_bases
TGTTGACGTTCTGGCTGGCCGTCGCCACCCTTAGCGCTCTGGCCTTAGTGGCCGGGGCAGTGTTGGGCTTTGCCGCCCGT
CGTTTTCAGGTAAAAACAGA

Product: Na(+)-translocating NADH-quinone reductase subunit E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MNGYLMLFIGTVLVNNFVLVKFLGLCPFMGVSKKVETALGMGMATTFVMTLASICAWLINDFILLPLNIPYLRTLAFIFI
IAVVVQFTELVVRKTSPALYRLLGIFLPLITTNCAVLGVALLNVNENHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
ADVPAPFKGSSIALITAGLMSLAFMGFTGLVKV

Sequences:

>Translated_193_residues
MNGYLMLFIGTVLVNNFVLVKFLGLCPFMGVSKKVETALGMGMATTFVMTLASICAWLINDFILLPLNIPYLRTLAFIFI
IAVVVQFTELVVRKTSPALYRLLGIFLPLITTNCAVLGVALLNVNENHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
ADVPAPFKGSSIALITAGLMSLAFMGFTGLVKV
>Mature_193_residues
MNGYLMLFIGTVLVNNFVLVKFLGLCPFMGVSKKVETALGMGMATTFVMTLASICAWLINDFILLPLNIPYLRTLAFIFI
IAVVVQFTELVVRKTSPALYRLLGIFLPLITTNCAVLGVALLNVNENHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
ADVPAPFKGSSIALITAGLMSLAFMGFTGLVKV

Specific function: May be part of a membrane complex involved in electron transport

COG id: COG4657

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family

Homologues:

Organism=Escherichia coli, GI1787914, Length=192, Percent_Identity=87.5, Blast_Score=337, Evalue=4e-94,
Organism=Escherichia coli, GI1787919, Length=142, Percent_Identity=37.3239436619718, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFA_SODGM (Q2NSZ5)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455135.1
- STRING:   Q2NSZ5
- GeneID:   3867272
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1455
- NMPDR:   fig|343509.6.peg.2992
- eggNOG:   COG4657
- HOGENOM:   HBG624915
- OMA:   LRTIAFI
- PhylomeDB:   Q2NSZ5
- ProtClustDB:   PRK05151
- BioCyc:   SGLO343509:SG1455-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00459
- InterPro:   IPR003667
- InterPro:   IPR011293
- PIRSF:   PIRSF006102
- TIGRFAMs:   TIGR01943

Pfam domain/function: PF02508 Rnf-Nqr

EC number: NA

Molecular weight: Translated: 20851; Mature: 20851

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0xeec87b8)-; HASH(0xf15738c)-; HASH(0xf4d4d74)-; HASH(0xf82cf84)-; HASH(0xf4bdbe4)-; HASH(0xf84a5c0)-;

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGYLMLFIGTVLVNNFVLVKFLGLCPFMGVSKKVETALGMGMATTFVMTLASICAWLIN
CCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFILLPLNIPYLRTLAFIFIIAVVVQFTELVVRKTSPALYRLLGIFLPLITTNCAVLGVA
HHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLNVNENHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAVADVPAPFKGSSIALITAGLM
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SLAFMGFTGLVKV
HHHHHHHHHHHCH
>Mature Secondary Structure
MNGYLMLFIGTVLVNNFVLVKFLGLCPFMGVSKKVETALGMGMATTFVMTLASICAWLIN
CCCEEHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFILLPLNIPYLRTLAFIFIIAVVVQFTELVVRKTSPALYRLLGIFLPLITTNCAVLGVA
HHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LLNVNENHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAVADVPAPFKGSSIALITAGLM
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
SLAFMGFTGLVKV
HHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA