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The map label for this gene is rnfB

Identifier: 85059432

GI number: 85059432

Start: 2428675

End: 2429247

Strand: Reverse

Name: rnfB

Synonym: SG1454

Alternate gene names: 85059432

Gene position: 2429247-2428675 (Counterclockwise)

Preceding gene: 85059433

Following gene: 85059431

Centisome position: 58.24

GC content: 60.91

Gene sequence:

>573_bases
ATGTTGACGTTCTGGCTGGCCGTCGCCACCCTTAGCGCTCTGGCCTTAGTGGCCGGGGCAGTGTTGGGCTTTGCCGCCCG
TCGTTTTCAGGTAAAAACAGATCCGGTGGCCGAGCGCATCGATGCCCTGCTGCCGCAAAGCCAATGCGCCCAATGCGGCT
ACCCCGGTTGCCGTCCTTATGCCGAAGCGGTCGCCGGCGGCGCCCCGATCAATAAATGTGTGCCTGGCGGCGAGGCGGTA
ATGTTGAAAATCGCCGCGCAGCTCAGCGTCGATCCCCAGCCGATGGATGGCGAGGCCTCCAGCCGCCCTGAATCGCGCGT
GGCCTGGATAGACGAAGGCAATTGCATCGGCTGCACCAAATGCATTCAAGCTTGTCCGGTGGATGCCATCGTCGGCGCCA
CGCGCGCCGTACATACGGTGGTGAGCGATCTCTGTACCGGCTGCGATCTCTGTGTAGCGCCCTGTCCTACCAACTGTATT
GAAATGCGCCCGCTTGCCATCACCCCCACCAGCTGGAAGTGGGATCTGCGTACCATACCTATTACGGTCATCCAGCAGGA
GCAACATGTTTAA

Upstream 100 bases:

>100_bases
CGGATGTTCCCGCGCCCTTTAAGGGCTCCTCTATCGCTCTCATTACCGCCGGGTTAATGTCGCTGGCCTTTATGGGCTTT
ACCGGACTGGTGAAGGTCTG

Downstream 100 bases:

>100_bases
TTTATTGTCTGCCCTGCGAAAACAGCGACTATGGGATTTTCCGGGCGGTATCCATCCCCCCGAGATGAAAAGCCAGTCCA
GCAATGTCCCTCTGCGGTCC

Product: electron transport complex protein RnfB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MLTFWLAVATLSALALVAGAVLGFAARRFQVKTDPVAERIDALLPQSQCAQCGYPGCRPYAEAVAGGAPINKCVPGGEAV
MLKIAAQLSVDPQPMDGEASSRPESRVAWIDEGNCIGCTKCIQACPVDAIVGATRAVHTVVSDLCTGCDLCVAPCPTNCI
EMRPLAITPTSWKWDLRTIPITVIQQEQHV

Sequences:

>Translated_190_residues
MLTFWLAVATLSALALVAGAVLGFAARRFQVKTDPVAERIDALLPQSQCAQCGYPGCRPYAEAVAGGAPINKCVPGGEAV
MLKIAAQLSVDPQPMDGEASSRPESRVAWIDEGNCIGCTKCIQACPVDAIVGATRAVHTVVSDLCTGCDLCVAPCPTNCI
EMRPLAITPTSWKWDLRTIPITVIQQEQHV
>Mature_190_residues
MLTFWLAVATLSALALVAGAVLGFAARRFQVKTDPVAERIDALLPQSQCAQCGYPGCRPYAEAVAGGAPINKCVPGGEAV
MLKIAAQLSVDPQPMDGEASSRPESRVAWIDEGNCIGCTKCIQACPVDAIVGATRAVHTVVSDLCTGCDLCVAPCPTNCI
EMRPLAITPTSWKWDLRTIPITVIQQEQHV

Specific function: May be part of a membrane complex involved in electron transport

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Escherichia coli, GI1787915, Length=191, Percent_Identity=68.0628272251309, Blast_Score=270, Evalue=3e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFB_SODGM (Q2NSZ6)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455134.1
- ProteinModelPortal:   Q2NSZ6
- SMR:   Q2NSZ6
- STRING:   Q2NSZ6
- GeneID:   3867271
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1454
- NMPDR:   fig|343509.6.peg.2991
- eggNOG:   COG2878
- HOGENOM:   HBG549899
- OMA:   GFASERF
- PhylomeDB:   Q2NSZ6
- ProtClustDB:   PRK05113
- BioCyc:   SGLO343509:SG1454-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00463
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202
- PIRSF:   PIRSF005784
- TIGRFAMs:   TIGR01944

Pfam domain/function: PF00037 Fer4; PF04060 FeS

EC number: NA

Molecular weight: Translated: 20093; Mature: 20093

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
8.9 %Cys+Met (Translated Protein)
6.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
8.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTFWLAVATLSALALVAGAVLGFAARRFQVKTDPVAERIDALLPQSQCAQCGYPGCRPY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHCCHHHHHHCCCCCCCHH
AEAVAGGAPINKCVPGGEAVMLKIAAQLSVDPQPMDGEASSRPESRVAWIDEGNCIGCTK
HHHHCCCCCCHHCCCCCHHEEEEEHHHHCCCCCCCCCCCCCCCCCEEEEECCCCEECHHH
CIQACPVDAIVGATRAVHTVVSDLCTGCDLCVAPCPTNCIEMRPLAITPTSWKWDLRTIP
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEECCCEEECCCCCEEEEEECC
ITVIQQEQHV
EEEECCCCCC
>Mature Secondary Structure
MLTFWLAVATLSALALVAGAVLGFAARRFQVKTDPVAERIDALLPQSQCAQCGYPGCRPY
CHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHCCHHHHHHCCCCCCCHH
AEAVAGGAPINKCVPGGEAVMLKIAAQLSVDPQPMDGEASSRPESRVAWIDEGNCIGCTK
HHHHCCCCCCHHCCCCCHHEEEEEHHHHCCCCCCCCCCCCCCCCCEEEEECCCCEECHHH
CIQACPVDAIVGATRAVHTVVSDLCTGCDLCVAPCPTNCIEMRPLAITPTSWKWDLRTIP
HHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCEECCCEEECCCCCEEEEEECC
ITVIQQEQHV
EEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA