Click here to switch to the map view.

The map label for this gene is cls

Identifier: 85059356

GI number: 85059356

Start: 2287053

End: 2288513

Strand: Reverse

Name: cls

Synonym: SG1378

Alternate gene names: 85059356

Gene position: 2288513-2287053 (Counterclockwise)

Preceding gene: 85059357

Following gene: 85059350

Centisome position: 54.87

GC content: 57.02

Gene sequence:

>1461_bases
ATGACAACGCTTTATACCGTAATCAACTGGCTATTGCTATTCGGCTATTGGTTGTTGATCGCAGGCGTCACCCTGCGGGT
TATGATGAAACGTCGCGCGGTGCCCTCCGCCATGGCCTGGCTACTAGTCATCTATATTCTCCCCCTGGTGGGAATTATTA
CTTATCTGCTGTTCGGCGAACTCAACCTGGGTAAACGCCGCGCCGAACGCAGTAAGGCCCTCTGGCCGTCCACCGCCAAG
TGGATTGAAGATCTAAAAGGTTGTCGCCGCATTTTTGCCAGTGAAAACAGCGAAGTGGCCCGGGCGTTATTTCAACTGTG
CGAGCACCGTCAGGGCATGGGTGGCCTGAAGGGCAATCAGATGCAGCTGCTGACCAGCAGCGATGATGCCCTGCGGGCGC
TGGTCCGCGATATCGGCCTGGCGCAAAAAAATATTGAGATAGTGTTCTATATCTGGCAGAGCGGCGGCCTAGTGGACCAG
GTGGCGGAGGCGTTGATGGCGGTGGCGCGGCGCGGCGTGCGCTGCCGGCTAATGCTCGATTCCGCCGGTAGCGTGGACTT
TTTCCGCAGTCCCTACCCCGCGCTGATGCGCGCCGCCGGAGTGCATGTCGTAGAAGCCCTGCACGTCAGCCTCCTGCGGG
TCTTTCTGCGCCGCATGGATTTGCGACAGCATCGCAAAATGGTGCTGATTGACAATTATGTCGCCTACACCGGTAGTATG
AACATGGTGGATCCGCGTTTTTTCAAACAGGACGCCGGCGTCGGCCAATGGATCGATATCATGGCGCGCATGGAGGGGCC
GGTTGCGACCGCGATGGGGATCATTTTCTCCTGCGACTGGGAAATTGAAACCGGGGAACGCATCCTGCCGCCGCTGCCCG
ACGTCAATATCATGCCGTTTGAACAGGCCACCGGACACACCATCCAAGTGATCGCGTCGGGTCCCGGTTTCCCCGAGGGC
GTCATTCATCAGGCGTTGCTCACCTCGATTTATGCGGCGCGCGAACAACTGGTGATGACCACGCCCTACCTGGTGCCGAG
CGACGATTTGCTCCAGGCCATTTGTACCGCGGCACAGCTCGGCGTGGAAGTCCATATTATCGTTCCACGCCACAACGACT
CCATGTTGGTAGGCTGGGCCAGCCGGGCCTTTTTTGCCGAACTACTGGAAGCCGGAGTGCTGATCCATCAGTTTGAGGGC
GGTCTGCTGCATACCAAGAGTGTACTGGTCGACGGTCAATTGAGTCTGGTGGGGACGGTAAATCTGGATATGCGCAGCCT
ATGGCTTAATTTTGAGATAACCCTGGTCATCGACGATGACGATTTTGGCAGCGATTTGGCACGGGTACAGGAGGACTATA
TCGCCCGCTCACGGCTCATTGAACCCAAAGTCTGGTCGAAGCGTCCCTACTGGCAGCGTATTGTCGAGCGGCTGTTCTAC
TTTTTCAGTCCATTGCTGTAA

Upstream 100 bases:

>100_bases
GCGGATGCAGCGCATTATGCGCCGGGTAAAACAATTTCCTCTTTGCGCCCCTTATGCGTAAACTCAGGCTAACGCTCCGC
GAACAGGATTTTTCGCCCCT

Downstream 100 bases:

>100_bases
TTTTACCGGACGGCATGCTGTAATAGAGCCAATTCAGCTAACGGGACAGTAATGATGGATGTGGATAATCGTCTGACGGA
TGATGAAACGCTCGAAGCGG

Product: cardiolipin synthetase

Products: NA

Alternate protein names: CL synthase

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTTLYTVINWLLLFGYWLLIAGVTLRVMMKRRAVPSAMAWLLVIYILPLVGIITYLLFGELNLGKRRAERSKALWPSTAK
WIEDLKGCRRIFASENSEVARALFQLCEHRQGMGGLKGNQMQLLTSSDDALRALVRDIGLAQKNIEIVFYIWQSGGLVDQ
VAEALMAVARRGVRCRLMLDSAGSVDFFRSPYPALMRAAGVHVVEALHVSLLRVFLRRMDLRQHRKMVLIDNYVAYTGSM
NMVDPRFFKQDAGVGQWIDIMARMEGPVATAMGIIFSCDWEIETGERILPPLPDVNIMPFEQATGHTIQVIASGPGFPEG
VIHQALLTSIYAAREQLVMTTPYLVPSDDLLQAICTAAQLGVEVHIIVPRHNDSMLVGWASRAFFAELLEAGVLIHQFEG
GLLHTKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDDFGSDLARVQEDYIARSRLIEPKVWSKRPYWQRIVERLFY
FFSPLL

Sequences:

>Translated_486_residues
MTTLYTVINWLLLFGYWLLIAGVTLRVMMKRRAVPSAMAWLLVIYILPLVGIITYLLFGELNLGKRRAERSKALWPSTAK
WIEDLKGCRRIFASENSEVARALFQLCEHRQGMGGLKGNQMQLLTSSDDALRALVRDIGLAQKNIEIVFYIWQSGGLVDQ
VAEALMAVARRGVRCRLMLDSAGSVDFFRSPYPALMRAAGVHVVEALHVSLLRVFLRRMDLRQHRKMVLIDNYVAYTGSM
NMVDPRFFKQDAGVGQWIDIMARMEGPVATAMGIIFSCDWEIETGERILPPLPDVNIMPFEQATGHTIQVIASGPGFPEG
VIHQALLTSIYAAREQLVMTTPYLVPSDDLLQAICTAAQLGVEVHIIVPRHNDSMLVGWASRAFFAELLEAGVLIHQFEG
GLLHTKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDDFGSDLARVQEDYIARSRLIEPKVWSKRPYWQRIVERLFY
FFSPLL
>Mature_485_residues
TTLYTVINWLLLFGYWLLIAGVTLRVMMKRRAVPSAMAWLLVIYILPLVGIITYLLFGELNLGKRRAERSKALWPSTAKW
IEDLKGCRRIFASENSEVARALFQLCEHRQGMGGLKGNQMQLLTSSDDALRALVRDIGLAQKNIEIVFYIWQSGGLVDQV
AEALMAVARRGVRCRLMLDSAGSVDFFRSPYPALMRAAGVHVVEALHVSLLRVFLRRMDLRQHRKMVLIDNYVAYTGSMN
MVDPRFFKQDAGVGQWIDIMARMEGPVATAMGIIFSCDWEIETGERILPPLPDVNIMPFEQATGHTIQVIASGPGFPEGV
IHQALLTSIYAAREQLVMTTPYLVPSDDLLQAICTAAQLGVEVHIIVPRHNDSMLVGWASRAFFAELLEAGVLIHQFEGG
LLHTKSVLVDGQLSLVGTVNLDMRSLWLNFEITLVIDDDDFGSDLARVQEDYIARSRLIEPKVWSKRPYWQRIVERLFYF
FSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI1787502, Length=486, Percent_Identity=78.3950617283951, Blast_Score=819, Evalue=0.0,
Organism=Escherichia coli, GI1787007, Length=371, Percent_Identity=27.4932614555256, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLS_SODGM (Q2NT72)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_455058.1
- ProteinModelPortal:   Q2NT72
- STRING:   Q2NT72
- GeneID:   3867253
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG1378
- NMPDR:   fig|343509.6.peg.2809
- eggNOG:   COG1502
- HOGENOM:   HBG694312
- OMA:   MIIYILP
- PhylomeDB:   Q2NT72
- ProtClustDB:   PRK01642
- BioCyc:   SGLO343509:SG1378-MONOMER
- HAMAP:   MF_00190
- InterPro:   IPR022924
- InterPro:   IPR001736
- SMART:   SM00155

Pfam domain/function: PF00614 PLDc

EC number: 2.7.8.-

Molecular weight: Translated: 54842; Mature: 54711

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS50035 PLD

Important sites: ACT_SITE 224-224 ACT_SITE 226-226 ACT_SITE 231-231 ACT_SITE 404-404 ACT_SITE 406-406 ACT_SITE 411-411

Signals:

None

Transmembrane regions:

HASH(0xe7db428)-; HASH(0xe052704)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLYTVINWLLLFGYWLLIAGVTLRVMMKRRAVPSAMAWLLVIYILPLVGIITYLLFGE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
LNLGKRRAERSKALWPSTAKWIEDLKGCRRIFASENSEVARALFQLCEHRQGMGGLKGNQ
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCC
MQLLTSSDDALRALVRDIGLAQKNIEIVFYIWQSGGLVDQVAEALMAVARRGVRCRLMLD
EEEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEC
SAGSVDFFRSPYPALMRAAGVHVVEALHVSLLRVFLRRMDLRQHRKMVLIDNYVAYTGSM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEECCC
NMVDPRFFKQDAGVGQWIDIMARMEGPVATAMGIIFSCDWEIETGERILPPLPDVNIMPF
CCCCCHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHEEECCEECCCCCCCCCCCCCCEECC
EQATGHTIQVIASGPGFPEGVIHQALLTSIYAAREQLVMTTPYLVPSDDLLQAICTAAQL
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHC
GVEVHIIVPRHNDSMLVGWASRAFFAELLEAGVLIHQFEGGLLHTKSVLVDGQLSLVGTV
CCEEEEEEECCCCCEEEEHHHHHHHHHHHHHCCEEEECCCCEEEEEEEEECCCEEEEEEE
NLDMRSLWLNFEITLVIDDDDFGSDLARVQEDYIARSRLIEPKVWSKRPYWQRIVERLFY
CCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
FFSPLL
HHHCCH
>Mature Secondary Structure 
TTLYTVINWLLLFGYWLLIAGVTLRVMMKRRAVPSAMAWLLVIYILPLVGIITYLLFGE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCC
LNLGKRRAERSKALWPSTAKWIEDLKGCRRIFASENSEVARALFQLCEHRQGMGGLKGNQ
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCC
MQLLTSSDDALRALVRDIGLAQKNIEIVFYIWQSGGLVDQVAEALMAVARRGVRCRLMLD
EEEEECCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCEEEEEEC
SAGSVDFFRSPYPALMRAAGVHVVEALHVSLLRVFLRRMDLRQHRKMVLIDNYVAYTGSM
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEECCC
NMVDPRFFKQDAGVGQWIDIMARMEGPVATAMGIIFSCDWEIETGERILPPLPDVNIMPF
CCCCCHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHEEECCEECCCCCCCCCCCCCCEECC
EQATGHTIQVIASGPGFPEGVIHQALLTSIYAAREQLVMTTPYLVPSDDLLQAICTAAQL
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHC
GVEVHIIVPRHNDSMLVGWASRAFFAELLEAGVLIHQFEGGLLHTKSVLVDGQLSLVGTV
CCEEEEEEECCCCCEEEEHHHHHHHHHHHHHCCEEEECCCCEEEEEEEEECCCEEEEEEE
NLDMRSLWLNFEITLVIDDDDFGSDLARVQEDYIARSRLIEPKVWSKRPYWQRIVERLFY
CCCCEEEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
FFSPLL
HHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA