Click here to switch to the map view.

The map label for this gene is purU

Identifier: 85059342

GI number: 85059342

Start: 2265869

End: 2266717

Strand: Reverse

Name: purU

Synonym: SG1364

Alternate gene names: 85059342

Gene position: 2266717-2265869 (Counterclockwise)

Preceding gene: 85059343

Following gene: 85059341

Centisome position: 54.34

GC content: 54.42

Gene sequence:

>849_bases
ATGCAGTCGCAAAATGTACAACGAAAAGTATTACGCACCCTGTGTCCGGATGCGAAAGGCCTGATTGCGCAAATCACCAG
CATTTGCTTCAAGCATCAACTCAATATCGTGCAAAACAACGAGTATGTTGACCATCATACCAGCCGCTTCTTTATGCGTA
CCGAATTGGAGGGGCTCTTCAATGATGCCGCGCTGCTGGCGGATCTGGACGAGGTCCTGCCCGCGGGAACGATTCGGGAA
CTCAATCATGCCGGCCGGCGCCGCATCGTCGTGCTGGTGACGAAAGAAGCCCACTGTCTGGGTGATTTGCTGATGAAAAG
CACCTATGGCGGCCTCGATGTGGATATTGCCGCGGTAATAGGCAACCACGAGACGCTGCGGGCGCTGGCGGAGCGGTTTG
ATATCCCCTTTCATCTGGTCAGCCACGACGGCTTCAGCCGTGAAGAGCATGATGCGCTCATGATGGCGTTGATTGACACG
TTCGCACCCGACTATGTGGTGCTGGCGAAGTACATGCGCGTGCTAACGCCGGCGTTTGTCCGTCACTATCCCAATCAAAT
CATCAATATTCATCATTCATTCCTGCCGGCGTTCATCGGTGCACGCCCCTACCATCAGGCCTATGAACGCGGGGTGAAAA
TCATCGGCGCCACCGCGCACTACGTTAACGACAATCTGGATGAAGGCCCTATCATTATCCAGGACGTCATTCACGTCGAT
CATACTTACACTGCGAAAGATATGATGCGTGCGGGCCGGGATGTCGAAAAAAATGTGCTGAGCCGGGCGCTGTACCGCGT
GCTTGCCCAGCGGGTCTTCGTTTACGGCAACCGGACCGTGATTTTCTAA

Upstream 100 bases:

>100_bases
CCTTAAGTACAAAAAGTGTTGTGAACAATAATCCGGCCGGTGTTTGCTCGCGGCCCGCTTCATGGCAAAAACTCACAGAC
AGGACAGGATCCGCAAGTCA

Downstream 100 bases:

>100_bases
TTTCGCCTCCGGCCGCGCGCGGCAAGCCTGCAGTCGCCGCGCGGAATTTTACACGACTGAAGAAAAAAACGGCAAAAGAA
TCGGGGTGCACGCGTTAGCG

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MQSQNVQRKVLRTLCPDAKGLIAQITSICFKHQLNIVQNNEYVDHHTSRFFMRTELEGLFNDAALLADLDEVLPAGTIRE
LNHAGRRRIVVLVTKEAHCLGDLLMKSTYGGLDVDIAAVIGNHETLRALAERFDIPFHLVSHDGFSREEHDALMMALIDT
FAPDYVVLAKYMRVLTPAFVRHYPNQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIIQDVIHVD
HTYTAKDMMRAGRDVEKNVLSRALYRVLAQRVFVYGNRTVIF

Sequences:

>Translated_282_residues
MQSQNVQRKVLRTLCPDAKGLIAQITSICFKHQLNIVQNNEYVDHHTSRFFMRTELEGLFNDAALLADLDEVLPAGTIRE
LNHAGRRRIVVLVTKEAHCLGDLLMKSTYGGLDVDIAAVIGNHETLRALAERFDIPFHLVSHDGFSREEHDALMMALIDT
FAPDYVVLAKYMRVLTPAFVRHYPNQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIIQDVIHVD
HTYTAKDMMRAGRDVEKNVLSRALYRVLAQRVFVYGNRTVIF
>Mature_282_residues
MQSQNVQRKVLRTLCPDAKGLIAQITSICFKHQLNIVQNNEYVDHHTSRFFMRTELEGLFNDAALLADLDEVLPAGTIRE
LNHAGRRRIVVLVTKEAHCLGDLLMKSTYGGLDVDIAAVIGNHETLRALAERFDIPFHLVSHDGFSREEHDALMMALIDT
FAPDYVVLAKYMRVLTPAFVRHYPNQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIIQDVIHVD
HTYTAKDMMRAGRDVEKNVLSRALYRVLAQRVFVYGNRTVIF

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=30.5263157894737, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=30.5263157894737, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=30.5263157894737, Blast_Score=97, Evalue=2e-20,
Organism=Escherichia coli, GI1787483, Length=278, Percent_Identity=82.0143884892086, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1788846, Length=154, Percent_Identity=37.012987012987, Blast_Score=95, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17567511, Length=187, Percent_Identity=32.0855614973262, Blast_Score=103, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24582400, Length=159, Percent_Identity=33.9622641509434, Blast_Score=90, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32056; Mature: 32056

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSQNVQRKVLRTLCPDAKGLIAQITSICFKHQLNIVQNNEYVDHHTSRFFMRTELEGLF
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHH
NDAALLADLDEVLPAGTIRELNHAGRRRIVVLVTKEAHCLGDLLMKSTYGGLDVDIAAVI
HHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCCEEHHHHH
GNHETLRALAERFDIPFHLVSHDGFSREEHDALMMALIDTFAPDYVVLAKYMRVLTPAFV
CCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RHYPNQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIIQDVIHVD
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHHHCC
HTYTAKDMMRAGRDVEKNVLSRALYRVLAQRVFVYGNRTVIF
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MQSQNVQRKVLRTLCPDAKGLIAQITSICFKHQLNIVQNNEYVDHHTSRFFMRTELEGLF
CCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHHHHHHHHHHHH
NDAALLADLDEVLPAGTIRELNHAGRRRIVVLVTKEAHCLGDLLMKSTYGGLDVDIAAVI
HHHHHHHHHHHHCCCCHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHCCCCCCEEHHHHH
GNHETLRALAERFDIPFHLVSHDGFSREEHDALMMALIDTFAPDYVVLAKYMRVLTPAFV
CCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
RHYPNQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDNLDEGPIIIQDVIHVD
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHHHCC
HTYTAKDMMRAGRDVEKNVLSRALYRVLAQRVFVYGNRTVIF
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]