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The map label for this gene is cspD [H]

Identifier: 85059078

GI number: 85059078

Start: 1822618

End: 1822839

Strand: Reverse

Name: cspD [H]

Synonym: SG1100

Alternate gene names: 85059078

Gene position: 1822839-1822618 (Counterclockwise)

Preceding gene: 85059081

Following gene: 85059077

Centisome position: 43.7

GC content: 50.9

Gene sequence:

>222_bases
ATGGAGACGGGTAAAGTTAAATGGTTCAATAACGCCAAAGGTTTTGGTTTTATCTGCCCGGAAAACGGCGGCGAGGATAT
CTTTGCGCACTATTCAATGATCCATATGGACGGTTACCGGACGCTGAAGGCCGGGCAAGTCGTCAGATTCGAGGTTCATC
AGGGGCCCAAAGGGAACCATGCGAGCCTGATTGTGCCTGTTGAAGCCGAGACTGTTGCCTAA

Upstream 100 bases:

>100_bases
TCTAGAGTAGTAGGGGCATAAGCGGATGCGGGTAATCAGCCGCTTACGACTTCAACTATCCTCATCACATAAAAGCTTCG
GTTGCGAGGGATGTAGAAAC

Downstream 100 bases:

>100_bases
ATAACGCGCCTCGCAATTAAAGGTGTCATCGCATGAAATGCCAGCCTGTCGCTGGCATTTTTTTCTGCCGCTGATGCCTG
CATACTCTCAAGTTATAACA

Product: cold shock-like protein

Products: NA

Alternate protein names: CSP-D [H]

Number of amino acids: Translated: 73; Mature: 73

Protein sequence:

>73_residues
METGKVKWFNNAKGFGFICPENGGEDIFAHYSMIHMDGYRTLKAGQVVRFEVHQGPKGNHASLIVPVEAETVA

Sequences:

>Translated_73_residues
METGKVKWFNNAKGFGFICPENGGEDIFAHYSMIHMDGYRTLKAGQVVRFEVHQGPKGNHASLIVPVEAETVA
>Mature_73_residues
METGKVKWFNNAKGFGFICPENGGEDIFAHYSMIHMDGYRTLKAGQVVRFEVHQGPKGNHASLIVPVEAETVA

Specific function: Inhibits DNA replication at both initiation and elongation steps, most probably by binding to the opened, single- stranded regions at replication forks. Plays a regulatory role in chromosomal replication in nutrient-depleted cells [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=71, Percent_Identity=84.5070422535211, Blast_Score=131, Evalue=1e-32,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=50, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1789979, Length=65, Percent_Identity=49.2307692307692, Blast_Score=74, Evalue=3e-15,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=48.4375, Blast_Score=73, Evalue=4e-15,
Organism=Escherichia coli, GI1787839, Length=66, Percent_Identity=50, Blast_Score=73, Evalue=5e-15,
Organism=Escherichia coli, GI2367114, Length=65, Percent_Identity=46.1538461538462, Blast_Score=71, Evalue=2e-14,
Organism=Escherichia coli, GI1787834, Length=63, Percent_Identity=49.2063492063492, Blast_Score=69, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24658520, Length=61, Percent_Identity=44.2622950819672, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 8064; Mature: 8064

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METGKVKWFNNAKGFGFICPENGGEDIFAHYSMIHMDGYRTLKAGQVVRFEVHQGPKGNH
CCCCCEEEECCCCCEEEECCCCCCCCEEEEEEEEEECCCEEECCCEEEEEEECCCCCCCC
ASLIVPVEAETVA
CEEEEEECCEECC
>Mature Secondary Structure
METGKVKWFNNAKGFGFICPENGGEDIFAHYSMIHMDGYRTLKAGQVVRFEVHQGPKGNH
CCCCCEEEECCCCCEEEECCCCCCCCEEEEEEEEEECCCEEECCCEEEEEEECCCCCCCC
ASLIVPVEAETVA
CEEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]