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The map label for this gene is intE [H]

Identifier: 85059065

GI number: 85059065

Start: 1797144

End: 1797914

Strand: Reverse

Name: intE [H]

Synonym: SG1087

Alternate gene names: 85059065

Gene position: 1797914-1797144 (Counterclockwise)

Preceding gene: 85059066

Following gene: 85059064

Centisome position: 43.1

GC content: 50.19

Gene sequence:

>771_bases
ATGGCACGCCGGAGAAGCCATAAGAACCGCGATTTGCCGCCAAACCTGCAACCCCGCAATGGCGGCTACATCTGCTACCG
TGACCCACGCACGGGAAAAGAATATGGCCTAGGATGCAATCGTCGCGAGGCCATTACGCAAGCTATCGCGGCTAATATGG
CGATTTATGGCTCATCAAAGCAATCACCGCTCGTTGACAGCATCAGCGGCACACAGGCGACCACAGTTAACGAATGGACT
ACACGTTATCTGGAAATATTGGAGAGCCGAAAGCTTAAGCCGAAAACGATGAGTGAGTATAATAAATACCTGAAGGCCGT
TAACGCCTACTTGGGCAAGTTTGCTATTCAGAAAGTAGAAACCAAAGACATTGCCGACTTCCTGTACCAATGGGCAAAGG
CGGGGAAAGTGACAATGGCGAACTGCATCAGAGCGTTGTTACGTGACCTGTTTCGTGAAGCGATTGCTGAGGGGCTGCTC
AAAAGCAATCCAGCCGAAGCCACCCGAAACCAACGTATAGATATTCAACGGGAACGCATAACCTTGGATGCGTGGAAGCT
TATACGCAAGGCAGCGGATACATTACCTGCATGGGTCGGCCCTTGCCTTGATCTTGCTTTAGTCACCGGTCAACGCTTAG
GGGATATACAAGCACTACGCTGGGAGGATATCAAAGACGGACGTCTTTACATTAAACAGGCGAAAACAGGCATGATTGCT
TGCGTTCTCCGAGACAACCCGCATCGACTGTCTCAACTTATGTCTTTCTGA

Upstream 100 bases:

>100_bases
CAGGAATATTTGGTTGACGAAACGGCCACAAGGCTAAGTCGTAACGATACATTTTACCCGTCTAGCGCAACTCCACCATC
ATTAGTATCGAGGATAAAAC

Downstream 100 bases:

>100_bases
TGTACTAAACCGGTTTAGGACATTAAACAACAGCGGAGCGCATATTGTCGCCGATGCAGGACATGAGAAATTTTCTGCGA
AAACACTCTCCGCTGGATTT

Product: putative phage integrase

Products: NA

Alternate protein names: Int(Lambda); Prophage e14 integrase [H]

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MARRRSHKNRDLPPNLQPRNGGYICYRDPRTGKEYGLGCNRREAITQAIAANMAIYGSSKQSPLVDSISGTQATTVNEWT
TRYLEILESRKLKPKTMSEYNKYLKAVNAYLGKFAIQKVETKDIADFLYQWAKAGKVTMANCIRALLRDLFREAIAEGLL
KSNPAEATRNQRIDIQRERITLDAWKLIRKAADTLPAWVGPCLDLALVTGQRLGDIQALRWEDIKDGRLYIKQAKTGMIA
CVLRDNPHRLSQLMSF

Sequences:

>Translated_256_residues
MARRRSHKNRDLPPNLQPRNGGYICYRDPRTGKEYGLGCNRREAITQAIAANMAIYGSSKQSPLVDSISGTQATTVNEWT
TRYLEILESRKLKPKTMSEYNKYLKAVNAYLGKFAIQKVETKDIADFLYQWAKAGKVTMANCIRALLRDLFREAIAEGLL
KSNPAEATRNQRIDIQRERITLDAWKLIRKAADTLPAWVGPCLDLALVTGQRLGDIQALRWEDIKDGRLYIKQAKTGMIA
CVLRDNPHRLSQLMSF
>Mature_255_residues
ARRRSHKNRDLPPNLQPRNGGYICYRDPRTGKEYGLGCNRREAITQAIAANMAIYGSSKQSPLVDSISGTQATTVNEWTT
RYLEILESRKLKPKTMSEYNKYLKAVNAYLGKFAIQKVETKDIADFLYQWAKAGKVTMANCIRALLRDLFREAIAEGLLK
SNPAEATRNQRIDIQRERITLDAWKLIRKAADTLPAWVGPCLDLALVTGQRLGDIQALRWEDIKDGRLYIKQAKTGMIAC
VLRDNPHRLSQLMSF

Specific function: Integrase from the cryptic lambdoic prophage e14. Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the h

COG id: COG0582

COG function: function code L; Integrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family [H]

Homologues:

Organism=Escherichia coli, GI1787386, Length=248, Percent_Identity=36.2903225806452, Blast_Score=117, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016177
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR015094
- InterPro:   IPR023109
- InterPro:   IPR013087 [H]

Pfam domain/function: PF09003 Phage_integ_N; PF00589 Phage_integrase [H]

EC number: NA

Molecular weight: Translated: 29104; Mature: 28973

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRRSHKNRDLPPNLQPRNGGYICYRDPRTGKEYGLGCNRREAITQAIAANMAIYGSSK
CCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHEECCCC
QSPLVDSISGTQATTVNEWTTRYLEILESRKLKPKTMSEYNKYLKAVNAYLGKFAIQKVE
CCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TKDIADFLYQWAKAGKVTMANCIRALLRDLFREAIAEGLLKSNPAEATRNQRIDIQRERI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHH
TLDAWKLIRKAADTLPAWVGPCLDLALVTGQRLGDIQALRWEDIKDGRLYIKQAKTGMIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHCCCCCCCCCEEEEECCCCEEE
CVLRDNPHRLSQLMSF
EEECCCHHHHHHHHCC
>Mature Secondary Structure 
ARRRSHKNRDLPPNLQPRNGGYICYRDPRTGKEYGLGCNRREAITQAIAANMAIYGSSK
CCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCCCHHHHHHHHHHHHHHEECCCC
QSPLVDSISGTQATTVNEWTTRYLEILESRKLKPKTMSEYNKYLKAVNAYLGKFAIQKVE
CCCCHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TKDIADFLYQWAKAGKVTMANCIRALLRDLFREAIAEGLLKSNPAEATRNQRIDIQRERI
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCCCHHHHH
TLDAWKLIRKAADTLPAWVGPCLDLALVTGQRLGDIQALRWEDIKDGRLYIKQAKTGMIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCHHHHCCCCCCCCCEEEEECCCCEEE
CVLRDNPHRLSQLMSF
EEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]