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The map label for this gene is asnB

Identifier: 85058791

GI number: 85058791

Start: 1372556

End: 1374217

Strand: Reverse

Name: asnB

Synonym: SG0813

Alternate gene names: 85058791

Gene position: 1374217-1372556 (Counterclockwise)

Preceding gene: 85058792

Following gene: 85058789

Centisome position: 32.95

GC content: 57.28

Gene sequence:

>1662_bases
ATGTGTTCTATTTTTGGGGTACTGGATCTGAAGACCGATCCGGTTGAATTACGTAAACAGGCGCTGGAATGCTCTCGCCT
GATGCGTCACCGCGGCCCCGATTGGTCCGGCGTCTATGCGGGCGAAAAAGCGATATTGGCCCATGAGCGTTTGTCCATCG
TCGACGTCAACACCGGCGCCCAACCTCTTTACAACGCGGCGCACACGCACGTGTTGGCGGTAAACGGCGAGATTTATAAG
CATCAGCTCATCCGCGAACAGCTGGGCGACCGCTACGCCTTTCAGACCGGTTCCGACTGCGAAGTCATTCTGGCCCTGTA
TCAGGAAAAAGGCCCGGAATTTCCGGACGAATTGCGCGGCATGTTCGCCTTTATCCTGTACGACACGGAAAAAGACGCCT
ATTTAATCGGCCGCGATCACATGGGCATCATCCCGCTGTATATGGGTCATGACGAGCACGGTAACCTGTATGTCGCGTCG
GAAATGAAAGCGCTGGTCCCGGTCTGTCGCACGCTCCAAGAGTTCCCGCCGGGACACTACCTCTGGAGCCAGGACGGTGA
ACTGCGTCCCTACTATCAGCGCGATTGGTTCGATTACGATCGGGTGAAAGACAATGTCACCGACAAAGCGGCGCTGAAGG
CGGCTCTGGAAGAATCGGTGAAAAGCCATCTGATGTCTGATGTGCCCTACGGCGTGCTGCTGTCCGGCGGGCTTGATTCA
TCCATAATCTCTGCCATCACTAAGAAGTATGCCGCCCGTCGGGTGGAAGATCACGCGCAAAGCGAAGCCTGGTGGCCGCA
GCTGCACTCTTTCGCCGTGGGCCTGGAAGGCTCGCCGGATCTGAGCGCCGCCAGCAAAGTGGCGGCGGCGCTGGGCACGG
TGCACCATGAAATTCATTTCACGGTGCAAGAAGGTATGGACGCGATCCGCGACGTGATTTACCACGTCGAAACCTATGAC
GTCACGACCATCCGCGCCTCGACGCCGATGTACCTGATGTCGCGCAAAATCAAGGCGATGGGCATCAAAATGGTCCTGTC
CGGCGAAGGCGCCGATGAAGTGTTCGGCGGCTATCTCTATTTCCACAAGGCGCCGAACGCGAAAGAGTTCCATGAAGAAC
TGGTGCGTAAACTGCAGGCCTTGCATATGTATGACTGCGCCCGCGCCAACAAAGCGATGTCCGCCTGGGGCGTCGAGGCA
CGCGTCCCCTTCCTCGACAAGCAGTTCCTGGATGTGGCGATGCGTATTAACCCGCAGGATAAAATGTGCGGCAACGGCAA
AATTGAGAAACACGTGCTGCGCGAATGCTTTGCCGATTATCTGCCGGAGAGCGTTGCCTGGCGGCAGAAGGAACAGTTTT
CCGACGGCGTGGGCTACAGCTGGATTGACAGCCTCAAAGTCATTGCCGCGGAGCAAATCAGCGATCAACAGCTGGCCAAC
GCCCACTTCCGCTTCCCCTATAACACGCCGACGTCCAAAGAAGGCTATCTGTACCGGGAAATTTTCACCGAGCTGTTCCC
ACTGCCAAGCGCCGCGGAATGCGTACCTGGGGGCCCGTCGGTGGCCTGCTCTTCCGCCAAGGCCATCGAATGGGACAAAT
CGTTCAAAAAAATGGACGATCCCTCTGGTCGCGCGGTAGGCGTACACGCTTCGGCCTATTGA

Upstream 100 bases:

>100_bases
TTTCCACCTGGATATTGAATTGTTGGGTCGTTTTGATGCTAAATCGTTGCATATGTGACGGCGCAGGGCGACCGCCGGAA
ATCATCATGAGGGAAAAATT

Downstream 100 bases:

>100_bases
TAGCCGCCTGCGAGAATGCCCTGCTGGCGTCCAGGACCGTTGGACAGACGGCCATCGGCCTCGGCCGGCAATGCTGAGTG
CATGGCGTCAAACCGCCGTG

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MCSIFGVLDLKTDPVELRKQALECSRLMRHRGPDWSGVYAGEKAILAHERLSIVDVNTGAQPLYNAAHTHVLAVNGEIYK
HQLIREQLGDRYAFQTGSDCEVILALYQEKGPEFPDELRGMFAFILYDTEKDAYLIGRDHMGIIPLYMGHDEHGNLYVAS
EMKALVPVCRTLQEFPPGHYLWSQDGELRPYYQRDWFDYDRVKDNVTDKAALKAALEESVKSHLMSDVPYGVLLSGGLDS
SIISAITKKYAARRVEDHAQSEAWWPQLHSFAVGLEGSPDLSAASKVAAALGTVHHEIHFTVQEGMDAIRDVIYHVETYD
VTTIRASTPMYLMSRKIKAMGIKMVLSGEGADEVFGGYLYFHKAPNAKEFHEELVRKLQALHMYDCARANKAMSAWGVEA
RVPFLDKQFLDVAMRINPQDKMCGNGKIEKHVLRECFADYLPESVAWRQKEQFSDGVGYSWIDSLKVIAAEQISDQQLAN
AHFRFPYNTPTSKEGYLYREIFTELFPLPSAAECVPGGPSVACSSAKAIEWDKSFKKMDDPSGRAVGVHASAY

Sequences:

>Translated_553_residues
MCSIFGVLDLKTDPVELRKQALECSRLMRHRGPDWSGVYAGEKAILAHERLSIVDVNTGAQPLYNAAHTHVLAVNGEIYK
HQLIREQLGDRYAFQTGSDCEVILALYQEKGPEFPDELRGMFAFILYDTEKDAYLIGRDHMGIIPLYMGHDEHGNLYVAS
EMKALVPVCRTLQEFPPGHYLWSQDGELRPYYQRDWFDYDRVKDNVTDKAALKAALEESVKSHLMSDVPYGVLLSGGLDS
SIISAITKKYAARRVEDHAQSEAWWPQLHSFAVGLEGSPDLSAASKVAAALGTVHHEIHFTVQEGMDAIRDVIYHVETYD
VTTIRASTPMYLMSRKIKAMGIKMVLSGEGADEVFGGYLYFHKAPNAKEFHEELVRKLQALHMYDCARANKAMSAWGVEA
RVPFLDKQFLDVAMRINPQDKMCGNGKIEKHVLRECFADYLPESVAWRQKEQFSDGVGYSWIDSLKVIAAEQISDQQLAN
AHFRFPYNTPTSKEGYLYREIFTELFPLPSAAECVPGGPSVACSSAKAIEWDKSFKKMDDPSGRAVGVHASAY
>Mature_553_residues
MCSIFGVLDLKTDPVELRKQALECSRLMRHRGPDWSGVYAGEKAILAHERLSIVDVNTGAQPLYNAAHTHVLAVNGEIYK
HQLIREQLGDRYAFQTGSDCEVILALYQEKGPEFPDELRGMFAFILYDTEKDAYLIGRDHMGIIPLYMGHDEHGNLYVAS
EMKALVPVCRTLQEFPPGHYLWSQDGELRPYYQRDWFDYDRVKDNVTDKAALKAALEESVKSHLMSDVPYGVLLSGGLDS
SIISAITKKYAARRVEDHAQSEAWWPQLHSFAVGLEGSPDLSAASKVAAALGTVHHEIHFTVQEGMDAIRDVIYHVETYD
VTTIRASTPMYLMSRKIKAMGIKMVLSGEGADEVFGGYLYFHKAPNAKEFHEELVRKLQALHMYDCARANKAMSAWGVEA
RVPFLDKQFLDVAMRINPQDKMCGNGKIEKHVLRECFADYLPESVAWRQKEQFSDGVGYSWIDSLKVIAAEQISDQQLAN
AHFRFPYNTPTSKEGYLYREIFTELFPLPSAAECVPGGPSVACSSAKAIEWDKSFKKMDDPSGRAVGVHASAY

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=524, Percent_Identity=38.1679389312977, Blast_Score=309, Evalue=4e-84,
Organism=Homo sapiens, GI168229252, Length=524, Percent_Identity=38.1679389312977, Blast_Score=309, Evalue=4e-84,
Organism=Homo sapiens, GI168229250, Length=524, Percent_Identity=38.1679389312977, Blast_Score=309, Evalue=4e-84,
Organism=Homo sapiens, GI296010848, Length=513, Percent_Identity=38.5964912280702, Blast_Score=308, Evalue=9e-84,
Organism=Homo sapiens, GI296010852, Length=447, Percent_Identity=39.8210290827741, Blast_Score=283, Evalue=4e-76,
Organism=Homo sapiens, GI296010850, Length=447, Percent_Identity=39.8210290827741, Blast_Score=283, Evalue=4e-76,
Organism=Escherichia coli, GI1786889, Length=553, Percent_Identity=86.4376130198915, Blast_Score=1015, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147557, Length=568, Percent_Identity=42.4295774647887, Blast_Score=427, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI71993933, Length=559, Percent_Identity=42.397137745975, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17560178, Length=520, Percent_Identity=34.2307692307692, Blast_Score=281, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI25147560, Length=341, Percent_Identity=36.0703812316716, Blast_Score=193, Evalue=2e-49,
Organism=Saccharomyces cerevisiae, GI6321563, Length=581, Percent_Identity=48.3648881239243, Blast_Score=529, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6325403, Length=582, Percent_Identity=46.7353951890034, Blast_Score=516, Evalue=1e-147,
Organism=Drosophila melanogaster, GI45553209, Length=574, Percent_Identity=45.6445993031359, Blast_Score=483, Evalue=1e-136,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 62139; Mature: 62139

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIFGVLDLKTDPVELRKQALECSRLMRHRGPDWSGVYAGEKAILAHERLSIVDVNTGA
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEECCCC
QPLYNAAHTHVLAVNGEIYKHQLIREQLGDRYAFQTGSDCEVILALYQEKGPEFPDELRG
CHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHH
MFAFILYDTEKDAYLIGRDHMGIIPLYMGHDEHGNLYVASEMKALVPVCRTLQEFPPGHY
HEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCE
LWSQDGELRPYYQRDWFDYDRVKDNVTDKAALKAALEESVKSHLMSDVPYGVLLSGGLDS
EECCCCCCCCCHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
SIISAITKKYAARRVEDHAQSEAWWPQLHSFAVGLEGSPDLSAASKVAAALGTVHHEIHF
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHEEE
TVQEGMDAIRDVIYHVETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGADEVFGGYLY
EHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHCCEEEEEECCCCHHHHCCEEE
FHKAPNAKEFHEELVRKLQALHMYDCARANKAMSAWGVEARVPFLDKQFLDVAMRINPQD
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHEECCCC
KMCGNGKIEKHVLRECFADYLPESVAWRQKEQFSDGVGYSWIDSLKVIAAEQISDQQLAN
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHC
AHFRFPYNTPTSKEGYLYREIFTELFPLPSAAECVPGGPSVACSSAKAIEWDKSFKKMDD
CEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
PSGRAVGVHASAY
CCCCEEEEEECCC
>Mature Secondary Structure
MCSIFGVLDLKTDPVELRKQALECSRLMRHRGPDWSGVYAGEKAILAHERLSIVDVNTGA
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCEEEEEECCCC
QPLYNAAHTHVLAVNGEIYKHQLIREQLGDRYAFQTGSDCEVILALYQEKGPEFPDELRG
CHHHHHHCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHH
MFAFILYDTEKDAYLIGRDHMGIIPLYMGHDEHGNLYVASEMKALVPVCRTLQEFPPGHY
HEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHCCCCCE
LWSQDGELRPYYQRDWFDYDRVKDNVTDKAALKAALEESVKSHLMSDVPYGVLLSGGLDS
EECCCCCCCCCHHCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCH
SIISAITKKYAARRVEDHAQSEAWWPQLHSFAVGLEGSPDLSAASKVAAALGTVHHEIHF
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHEEE
TVQEGMDAIRDVIYHVETYDVTTIRASTPMYLMSRKIKAMGIKMVLSGEGADEVFGGYLY
EHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHCCEEEEEECCCCHHHHCCEEE
FHKAPNAKEFHEELVRKLQALHMYDCARANKAMSAWGVEARVPFLDKQFLDVAMRINPQD
EECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHEECCCC
KMCGNGKIEKHVLRECFADYLPESVAWRQKEQFSDGVGYSWIDSLKVIAAEQISDQQLAN
CCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCHHHHHC
AHFRFPYNTPTSKEGYLYREIFTELFPLPSAAECVPGGPSVACSSAKAIEWDKSFKKMDD
CEEECCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCC
PSGRAVGVHASAY
CCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]