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The map label for this gene is holA

Identifier: 85058779

GI number: 85058779

Start: 1353900

End: 1354931

Strand: Reverse

Name: holA

Synonym: SG0801

Alternate gene names: 85058779

Gene position: 1354931-1353900 (Counterclockwise)

Preceding gene: 85058780

Following gene: 85058778

Centisome position: 32.48

GC content: 61.43

Gene sequence:

>1032_bases
ATGATTCGGCTGTATGCCGAACAGTTGGCTGCGCAATTACTGGAAGCACTGCGCCCATGTTATCTGCTGTTTGGCAACGA
TCCGCTGCTGCTACAGGAAAGCCAGGATCGCATTCGCGACCGGGCACAGGCGCAGCAATTCGACGAGCGTTTCAGCGTCA
CGCTGGACGCCGCCACCGATTGGGACGCGATTTTCAGTCTGTGCCAGGCGCGCAGTCTGTTCGCCAGTCGGCAAACGCTG
CTGCTTGTCCTGCCGGACGGCGGCATCAACGCCGCCATGGCGGAAAAAATGCTCCAGCTGGCGTCGCTGTTGCATGACGA
CGTGCTCTTGATCCTACGCGGCAGCAAGCTGACCCGCGCCACGGAGAACGACTCCTGGTTCAAGGCACTGAGTCAAAACG
CGGTGCTGGTGAGCTGCACCACGCCGGAGCAGGCGCAGCTGCCGCGCTGGGTGGCAAGACGTGCCGCGAGTATGAAGCTG
ACGCTGGATGATGCCGCCTGCCAGCTATTGTGTTATTGCTACGAGGGCAACCTGCTAGCGCTGGCCCAGGCGCTGGAACG
ATTGTCGCTGCTTTATCCTGACGGCAGTCTGACGCTGCCGCGGGTCGAAGCGGCGGTGAACGACGCCGCACACTTCACGC
CGTTTCACTGGGTCGACGCCGCGTTGGCGGACAAAAGCAAACGCGCCGCGCATATTTTGCGTCAGTTGCGGCTGGAGGCC
ACGGAGCCGGTCATCCTGTTGCGCAGCATCCAGCGCGAAGTCCTGCTGCTGCTGACGCTCAAACGGCAAACGGCCGTCGC
GCCGCTGCGTACCCTGTTCGACCGCCACAAGGTCTGGCAGAACCGCCGTTCGCTGCTGACGCAGGCCATAGGGCGGCTTA
CTCTGGCGCAGTTGCGCGATACGGTGGCACTGATGACGAAAATCGAACTGGCGCTCAAACAGGATTATGGCTACCCCGTA
TGGTCCGATCTGAACGCCCTGGCGTTATTGTTGTGCGGCAAATCGCTGCCCACGGCAATGTTTGATGTCTGA

Upstream 100 bases:

>100_bases
GATAAGGCGCTGGACGCAAAACTGAAACAGCAGCAGCCACCGCAGCCGGTGGCGCCGGCCTCCTGATGACCCTGTGTCTG
GTGAAACGGCGGTCAGCGTC

Downstream 100 bases:

>100_bases
ACGCGCGCTCACCGCCTTTTACGGAGGCACGTTCGACCCTATCCACTACGGCCACCTGCGGCCGGTCATCGCGCTGGCGC
GCCTTGTCAACCTGCAGCGG

Product: DNA polymerase III subunit delta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MIRLYAEQLAAQLLEALRPCYLLFGNDPLLLQESQDRIRDRAQAQQFDERFSVTLDAATDWDAIFSLCQARSLFASRQTL
LLVLPDGGINAAMAEKMLQLASLLHDDVLLILRGSKLTRATENDSWFKALSQNAVLVSCTTPEQAQLPRWVARRAASMKL
TLDDAACQLLCYCYEGNLLALAQALERLSLLYPDGSLTLPRVEAAVNDAAHFTPFHWVDAALADKSKRAAHILRQLRLEA
TEPVILLRSIQREVLLLLTLKRQTAVAPLRTLFDRHKVWQNRRSLLTQAIGRLTLAQLRDTVALMTKIELALKQDYGYPV
WSDLNALALLLCGKSLPTAMFDV

Sequences:

>Translated_343_residues
MIRLYAEQLAAQLLEALRPCYLLFGNDPLLLQESQDRIRDRAQAQQFDERFSVTLDAATDWDAIFSLCQARSLFASRQTL
LLVLPDGGINAAMAEKMLQLASLLHDDVLLILRGSKLTRATENDSWFKALSQNAVLVSCTTPEQAQLPRWVARRAASMKL
TLDDAACQLLCYCYEGNLLALAQALERLSLLYPDGSLTLPRVEAAVNDAAHFTPFHWVDAALADKSKRAAHILRQLRLEA
TEPVILLRSIQREVLLLLTLKRQTAVAPLRTLFDRHKVWQNRRSLLTQAIGRLTLAQLRDTVALMTKIELALKQDYGYPV
WSDLNALALLLCGKSLPTAMFDV
>Mature_343_residues
MIRLYAEQLAAQLLEALRPCYLLFGNDPLLLQESQDRIRDRAQAQQFDERFSVTLDAATDWDAIFSLCQARSLFASRQTL
LLVLPDGGINAAMAEKMLQLASLLHDDVLLILRGSKLTRATENDSWFKALSQNAVLVSCTTPEQAQLPRWVARRAASMKL
TLDDAACQLLCYCYEGNLLALAQALERLSLLYPDGSLTLPRVEAAVNDAAHFTPFHWVDAALADKSKRAAHILRQLRLEA
TEPVILLRSIQREVLLLLTLKRQTAVAPLRTLFDRHKVWQNRRSLLTQAIGRLTLAQLRDTVALMTKIELALKQDYGYPV
WSDLNALALLLCGKSLPTAMFDV

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=342, Percent_Identity=67.5438596491228, Blast_Score=448, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 38492; Mature: 38492

Theoretical pI: Translated: 8.05; Mature: 8.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRLYAEQLAAQLLEALRPCYLLFGNDPLLLQESQDRIRDRAQAQQFDERFSVTLDAATD
CEEHHHHHHHHHHHHHHCCEEEEECCCCEEECCHHHHHHHHHHHHHHHHHHEEEEECCCC
WDAIFSLCQARSLFASRQTLLLVLPDGGINAAMAEKMLQLASLLHDDVLLILRGSKLTRA
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
TENDSWFKALSQNAVLVSCTTPEQAQLPRWVARRAASMKLTLDDAACQLLCYCYEGNLLA
CCCCHHHHHHCCCCEEEEECCCCHHHCHHHHHHHHHHEEEEECHHHHHHHHHHCCCCHHH
LAQALERLSLLYPDGSLTLPRVEAAVNDAAHFTPFHWVDAALADKSKRAAHILRQLRLEA
HHHHHHHHHEECCCCCCCCHHHHHHHCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
TEPVILLRSIQREVLLLLTLKRQTAVAPLRTLFDRHKVWQNRRSLLTQAIGRLTLAQLRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVALMTKIELALKQDYGYPVWSDLNALALLLCGKSLPTAMFDV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MIRLYAEQLAAQLLEALRPCYLLFGNDPLLLQESQDRIRDRAQAQQFDERFSVTLDAATD
CEEHHHHHHHHHHHHHHCCEEEEECCCCEEECCHHHHHHHHHHHHHHHHHHEEEEECCCC
WDAIFSLCQARSLFASRQTLLLVLPDGGINAAMAEKMLQLASLLHDDVLLILRGSKLTRA
HHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCC
TENDSWFKALSQNAVLVSCTTPEQAQLPRWVARRAASMKLTLDDAACQLLCYCYEGNLLA
CCCCHHHHHHCCCCEEEEECCCCHHHCHHHHHHHHHHEEEEECHHHHHHHHHHCCCCHHH
LAQALERLSLLYPDGSLTLPRVEAAVNDAAHFTPFHWVDAALADKSKRAAHILRQLRLEA
HHHHHHHHHEECCCCCCCCHHHHHHHCCHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHCC
TEPVILLRSIQREVLLLLTLKRQTAVAPLRTLFDRHKVWQNRRSLLTQAIGRLTLAQLRD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVALMTKIELALKQDYGYPVWSDLNALALLLCGKSLPTAMFDV
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]