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The map label for this gene is speE

Identifier: 85058457

GI number: 85058457

Start: 833712

End: 834575

Strand: Reverse

Name: speE

Synonym: SG0479

Alternate gene names: 85058457

Gene position: 834575-833712 (Counterclockwise)

Preceding gene: 85058458

Following gene: 85058456

Centisome position: 20.01

GC content: 54.4

Gene sequence:

>864_bases
ATGTCCCATCAAGAAACCTGGCACGAAACGTTACACGATCATTTCGGCCAATATTTTACTGTGGATAATCTTCTGTACCG
GGATACAACCGGCCAGCAGGATTTGGTGATATTTGAAAACGCCGCGCTCGGCCGCGTCATGGCGTTGGACGGCGTGGTGC
AGACCACCGAGCGTGATGAGTTTATTTACCATGAAATGATGGCTCATGTACCGCTTATCGCGCATGGCGGTGCCAAAAAA
GTGCTGATCATCGGCGGCGGCGACGGCGGTATGCTACGCGAAGTCAGCCGGCATCCCGGCGTTGAACAGATAACGATGGT
TGAAATCGATGCCGGCGTAGTGACGTTCTGCAAGCAATACCTGCCGCGCCACAACGCCGGCGCGTTTGACGATCCGCGCT
TCAAGCTGGTGATCGCTGACGGGGTGAACTTTGTTTCCCAAAGCAGTGAGAAATTTGATGTCATCATTTCCGACTGCACC
GACCCTATCGGGCCGGGAGAAAGCCTATTTACCTCTAATTTTTATCAAGACTGCGCACGCTGTCTCAATGAGGGCGGCAT
TTTCGTCGCGCAGAACGGCGTGTGTTTTTTACAGCAGGATGAAGTGGTAAACAGCCATCGTAAACTTGGTCACTATTTTA
GCGACATCAGTTTTTATCAGGCGGCGGTGCCGACCTATTATGGCGGCATCATGACCTTCGCCTGGGCGAGCCAGAATCCG
GCGCTACGCCAGCTCGACACGGCGACCCTGGCGGCTCGTATCGACGAGACCGCGCTAACCTGCCGCTATTACAATGCGGC
GATCCATCACGGCAGCTTCGCCCTGCCGCAATATTTGCTGAATGCCTTATCAGCCTCCCGCTGA

Upstream 100 bases:

>100_bases
CTAGCCCCTGTGCACGAAATTTAACCGTGCAACGGCGGGCAGAGTTAGCTATCATATTGCGCTGTTTTTTCATGGCGGAC
TTGCCAAGGAGCAGCCAGAC

Downstream 100 bases:

>100_bases
AACGGTATCCATAAGGGGGTGAACCAAATTGCAGAAGCTAAAACTACACGGCTTTAATAACCTGACCAAGAGCCTGAGTT
TTTGTATTTACGATATTTGC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSHQETWHETLHDHFGQYFTVDNLLYRDTTGQQDLVIFENAALGRVMALDGVVQTTERDEFIYHEMMAHVPLIAHGGAKK
VLIIGGGDGGMLREVSRHPGVEQITMVEIDAGVVTFCKQYLPRHNAGAFDDPRFKLVIADGVNFVSQSSEKFDVIISDCT
DPIGPGESLFTSNFYQDCARCLNEGGIFVAQNGVCFLQQDEVVNSHRKLGHYFSDISFYQAAVPTYYGGIMTFAWASQNP
ALRQLDTATLAARIDETALTCRYYNAAIHHGSFALPQYLLNALSASR

Sequences:

>Translated_287_residues
MSHQETWHETLHDHFGQYFTVDNLLYRDTTGQQDLVIFENAALGRVMALDGVVQTTERDEFIYHEMMAHVPLIAHGGAKK
VLIIGGGDGGMLREVSRHPGVEQITMVEIDAGVVTFCKQYLPRHNAGAFDDPRFKLVIADGVNFVSQSSEKFDVIISDCT
DPIGPGESLFTSNFYQDCARCLNEGGIFVAQNGVCFLQQDEVVNSHRKLGHYFSDISFYQAAVPTYYGGIMTFAWASQNP
ALRQLDTATLAARIDETALTCRYYNAAIHHGSFALPQYLLNALSASR
>Mature_286_residues
SHQETWHETLHDHFGQYFTVDNLLYRDTTGQQDLVIFENAALGRVMALDGVVQTTERDEFIYHEMMAHVPLIAHGGAKKV
LIIGGGDGGMLREVSRHPGVEQITMVEIDAGVVTFCKQYLPRHNAGAFDDPRFKLVIADGVNFVSQSSEKFDVIISDCTD
PIGPGESLFTSNFYQDCARCLNEGGIFVAQNGVCFLQQDEVVNSHRKLGHYFSDISFYQAAVPTYYGGIMTFAWASQNPA
LRQLDTATLAARIDETALTCRYYNAAIHHGSFALPQYLLNALSASR

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family

Homologues:

Organism=Homo sapiens, GI63253298, Length=284, Percent_Identity=36.6197183098592, Blast_Score=202, Evalue=2e-52,
Organism=Escherichia coli, GI1786312, Length=285, Percent_Identity=75.4385964912281, Blast_Score=467, Evalue=1e-133,
Organism=Caenorhabditis elegans, GI25154106, Length=274, Percent_Identity=40.8759124087591, Blast_Score=190, Evalue=7e-49,
Organism=Caenorhabditis elegans, GI212645616, Length=323, Percent_Identity=34.984520123839, Blast_Score=172, Evalue=1e-43,
Organism=Saccharomyces cerevisiae, GI6323175, Length=294, Percent_Identity=37.4149659863946, Blast_Score=196, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6325326, Length=288, Percent_Identity=35.0694444444444, Blast_Score=184, Evalue=1e-47,
Organism=Drosophila melanogaster, GI24645443, Length=267, Percent_Identity=38.9513108614232, Blast_Score=197, Evalue=9e-51,
Organism=Drosophila melanogaster, GI21355941, Length=215, Percent_Identity=38.6046511627907, Blast_Score=156, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SPEE_SODGM (Q2NVS1)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_454159.1
- ProteinModelPortal:   Q2NVS1
- SMR:   Q2NVS1
- STRING:   Q2NVS1
- GeneID:   3868693
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0479
- NMPDR:   fig|343509.6.peg.1082
- eggNOG:   COG0421
- HOGENOM:   HBG635113
- OMA:   FVAQTES
- PhylomeDB:   Q2NVS1
- ProtClustDB:   PRK00811
- BioCyc:   SGLO343509:SG0479-MONOMER
- HAMAP:   MF_00198
- InterPro:   IPR001045
- PANTHER:   PTHR11558
- TIGRFAMs:   TIGR00417

Pfam domain/function: PF01564 Spermine_synth

EC number: =2.5.1.16

Molecular weight: Translated: 31881; Mature: 31750

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS01330 SPERMIDINE_SYNTHASE_1; PS51006 SPERMIDINE_SYNTHASE_2

Important sites: BINDING 33-33 BINDING 88-88 BINDING 108-108 BINDING 158-158 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHQETWHETLHDHFGQYFTVDNLLYRDTTGQQDLVIFENAALGRVMALDGVVQTTERDE
CCCHHHHHHHHHHHCCCEEEECCEEEECCCCCCCEEEEECCCCCHHHHHCCHHHCCCCHH
FIYHEMMAHVPLIAHGGAKKVLIIGGGDGGMLREVSRHPGVEQITMVEIDAGVVTFCKQY
HHHHHHHHHCCEEEECCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
LPRHNAGAFDDPRFKLVIADGVNFVSQSSEKFDVIISDCTDPIGPGESLFTSNFYQDCAR
CCCCCCCCCCCCCEEEEEECCCHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
CLNEGGIFVAQNGVCFLQQDEVVNSHRKLGHYFSDISFYQAAVPTYYGGIMTFAWASQNP
HHCCCCEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCC
ALRQLDTATLAARIDETALTCRYYNAAIHHGSFALPQYLLNALSASR
HHHHHHHHHHHHHHCCHHEEEEEEHHHHCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHQETWHETLHDHFGQYFTVDNLLYRDTTGQQDLVIFENAALGRVMALDGVVQTTERDE
CCHHHHHHHHHHHCCCEEEECCEEEECCCCCCCEEEEECCCCCHHHHHCCHHHCCCCHH
FIYHEMMAHVPLIAHGGAKKVLIIGGGDGGMLREVSRHPGVEQITMVEIDAGVVTFCKQY
HHHHHHHHHCCEEEECCCCEEEEEECCCCHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
LPRHNAGAFDDPRFKLVIADGVNFVSQSSEKFDVIISDCTDPIGPGESLFTSNFYQDCAR
CCCCCCCCCCCCCEEEEEECCCHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHH
CLNEGGIFVAQNGVCFLQQDEVVNSHRKLGHYFSDISFYQAAVPTYYGGIMTFAWASQNP
HHCCCCEEEEECCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCC
ALRQLDTATLAARIDETALTCRYYNAAIHHGSFALPQYLLNALSASR
HHHHHHHHHHHHHHCCHHEEEEEEHHHHCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA