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The map label for this gene is dam [C]

Identifier: 85058450

GI number: 85058450

Start: 821539

End: 822225

Strand: Reverse

Name: dam [C]

Synonym: SG0472

Alternate gene names: 85058450

Gene position: 822225-821539 (Counterclockwise)

Preceding gene: 85058451

Following gene: 85058449

Centisome position: 19.71

GC content: 55.75

Gene sequence:

>687_bases
ATGAAACCTATCCTGAAATGGGCGGGCGGCAAGTCTCGCCTGATGCCCCGCTTGCGCCCCTTCCTGCCGGCGGCTGACTG
TCTGGTCGAACCTTTTGTCGGCGGTGCCTCGGTTTTCCTCGCTACTGACTACCCTCGCTATGTGCTGGCAGACATTAACC
CGGACCTTATCAATTTCTACCAGGCGGTTGTTGATAGCCCGGAAAGCGTCATACAGGCAGCGCTGCCGCTGTTCAGGCAC
GCTAACACTTCTGTTGACTATTTTCGTTACCGCACCAATTTTGCCCAATGGCGAGCGACCAGGGGCATTAAACGTGCCGC
CGTGTTCCTTTACCTCAATCGCCATGGCTACAACGGCCTGTGCCGCTACAACCTGCGTGGCGAATTTAACATCCCGTATG
AACATTACAAAAAGCCCTATTTCCCCGCCGCAGAAATCCGCCTGTTCGCCGAAAAGGCGCGCGATACCCATATCCGGTTT
GTCTGCCTGCCCTTTGAGGAAACACTGGCGCTCCATGTAGATGAGCACTACGCAGTGTATACCGACCCGTCTTATTTCCC
GCCTGAAGACAGGGACGGCTTCACCGGTTATTACATCACGCCGTTGAGGAAATGCGCCTTACCGAGCTTCGCCGCGAGGA
CACTCTCCTGGCGAAAAAGCAGGTTGACGACCAGCTGGAAACGGTGA

Upstream 100 bases:

>100_bases
CACCACTCCCTTGAGGATGCCTGCTATCAGGCGCAGGTGGTGGCAGAAATCTGGCAGCGTCATTCCGACGCTTTTATCGA
ACATTGCTGAGGACATGCAG

Downstream 100 bases:

>100_bases
GTTCCAGTATTTACTGGCGCTTTGACCAGCGCGAAAAGCAAAAGGGCTTTGACAGCGCCCGCCGCTGGCTAACCGAGTCC
GTGGTTAAGGTGATGTTGCC

Product: putative DNA adenine methylase

Products: NA

Alternate protein names: M.Eco67Dam; ORF1-EC67 DAM [H]

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKPILKWAGGKSRLMPRLRPFLPAADCLVEPFVGGASVFLATDYPRYVLADINPDLINFYQAVVDSPESVIQAALPLFRH
ANTSVDYFRYRTNFAQWRATRGIKRAAVFLYLNRHGYNGLCRYNLRGEFNIPYEHYKKPYFPAAEIRLFAEKARDTHIRF
VCLPFEETLALHVDEHYAVYTDPSYFPPEDRDGFTGYYITPLRKCALPSFAARTLSWRKSRLTTSWKR

Sequences:

>Translated_228_residues
MKPILKWAGGKSRLMPRLRPFLPAADCLVEPFVGGASVFLATDYPRYVLADINPDLINFYQAVVDSPESVIQAALPLFRH
ANTSVDYFRYRTNFAQWRATRGIKRAAVFLYLNRHGYNGLCRYNLRGEFNIPYEHYKKPYFPAAEIRLFAEKARDTHIRF
VCLPFEETLALHVDEHYAVYTDPSYFPPEDRDGFTGYYITPLRKCALPSFAARTLSWRKSRLTTSWKR
>Mature_228_residues
MKPILKWAGGKSRLMPRLRPFLPAADCLVEPFVGGASVFLATDYPRYVLADINPDLINFYQAVVDSPESVIQAALPLFRH
ANTSVDYFRYRTNFAQWRATRGIKRAAVFLYLNRHGYNGLCRYNLRGEFNIPYEHYKKPYFPAAEIRLFAEKARDTHIRF
VCLPFEETLALHVDEHYAVYTDPSYFPPEDRDGFTGYYITPLRKCALPSFAARTLSWRKSRLTTSWKR

Specific function: Methylates DNA within the sequence GATC and protects the DNA from cleavage by the restriction endonuclease MboI. May play a regulatory role in the functions of the retron [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=200, Percent_Identity=48, Blast_Score=181, Evalue=3e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 26534; Mature: 26534

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPILKWAGGKSRLMPRLRPFLPAADCLVEPFVGGASVFLATDYPRYVLADINPDLINFY
CCCCCCCCCCHHHHCHHHHHCCCHHHHHHHCCCCCEEEEEEECCCCEEEECCCHHHHHHH
QAVVDSPESVIQAALPLFRHANTSVDYFRYRTNFAQWRATRGIKRAAVFLYLNRHGYNGL
HHHHCCHHHHHHHHHHHHHCCCCCHHHHEEHHHHHHHHHHCCHHEEEEEEEEECCCCCCE
CRYNLRGEFNIPYEHYKKPYFPAAEIRLFAEKARDTHIRFVCLPFEETLALHVDEHYAVY
EEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCEEEE
TDPSYFPPEDRDGFTGYYITPLRKCALPSFAARTLSWRKSRLTTSWKR
ECCCCCCCCCCCCCCEEEECHHHHHCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKPILKWAGGKSRLMPRLRPFLPAADCLVEPFVGGASVFLATDYPRYVLADINPDLINFY
CCCCCCCCCCHHHHCHHHHHCCCHHHHHHHCCCCCEEEEEEECCCCEEEECCCHHHHHHH
QAVVDSPESVIQAALPLFRHANTSVDYFRYRTNFAQWRATRGIKRAAVFLYLNRHGYNGL
HHHHCCHHHHHHHHHHHHHCCCCCHHHHEEHHHHHHHHHHCCHHEEEEEEEEECCCCCCE
CRYNLRGEFNIPYEHYKKPYFPAAEIRLFAEKARDTHIRFVCLPFEETLALHVDEHYAVY
EEEECCCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCEEEE
TDPSYFPPEDRDGFTGYYITPLRKCALPSFAARTLSWRKSRLTTSWKR
ECCCCCCCCCCCCCCEEEECHHHHHCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1701261 [H]