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The map label for this gene is ispB [H]

Identifier: 85058340

GI number: 85058340

Start: 656421

End: 657392

Strand: Reverse

Name: ispB [H]

Synonym: SG0362

Alternate gene names: 85058340

Gene position: 657392-656421 (Counterclockwise)

Preceding gene: 85058347

Following gene: 85058339

Centisome position: 15.76

GC content: 58.02

Gene sequence:

>972_bases
ATGAATATTGAACAAATTACTGAACTGACCGCGCAGGATATGGCGGAGGTCAACACGGCAATTCGCGAGCAGCTAAACTC
CGAGGTCGCCCTGATAAATCAGCTCGGCCACTACATTATCAGCGGCGGCGGCAAACGCATTCGGCCCATGATTGCGGTGC
TGGCAGCGCGGGCGCTGCATTATCAGGGTAAGCAGCACGTCACCGTCGCCGCACTGATTGAATTCATTCATACCGCCACG
CTGCTGCATGACGATGTGGTGGACGAATCCGACATGCGCCGCGGCAAAGCAACGGCCAACGCGGCGTTCGGCAACGCCGC
CAGCGTGCTGGTCGGCGATTTCATCTACACCCGCGCCTTCCAGATGATGACCGCACTGGAATCCCTGCGCGTATTAGAGC
TGATGTCCGAGGCGGTTAACATCATCGCTGAGGGCGAAGTCTTACAGTTGATGAACTGCAACGATCCGGATATTACCATC
GACAACTATATGCGGGTTATCTACAGCAAAACCGCGCGGCTATTCGAGGCGGCATCACAGTCATCCGCCATTTTAGCCGG
CGCCGATGCCTGCCAGGAAGCGGCGCTGCGCGACTACGGCCGCTATCTGGGTACCGCTTTTCAACTGATTGACGATCTGC
TGGATTACGGTGCGGACGGTAAAACGTTGGGGAAAAATACCGGCGATGACCTTAACGAAGGTAAGCCGACGCTTCCTTTA
TTGCACGCCATGCGTAACGGCACGCCAGAACAGGCGGCGCTGATCCGTCAGGCCATTGAACAGGGCAACGGGCGTCACCT
GCTGGAAACGGTGCTGAAAACGATGCACCAGTGCGATTCTTTGGGCTACACCCGCCGCTGCGCGGAAGAAGAGGCGGACA
AAGCTATCGCCTGTCTGGACTGCCTGTCCCCCTCGCCTTATCGCGATGCGCTGGAGAGCCTGGCCCACGTGGCGGTGCAG
CGCGACTCGTAA

Upstream 100 bases:

>100_bases
TCTCTTACGTTTTTCAAGTACAATCAGTAACACTCTTTCCGCCATCCGGTCCCGTGTACAGCATTACACTGACGGTATAC
CCTTAAACCGATAGAAAATA

Downstream 100 bases:

>100_bases
CCCGCTTGCGCAGCCAAAGGAATATATTTGGAAAATAATTGTCAGTGAACATTGACCCACGGCATCACGAATTGGTCGGC
GCCTCCCACGTACCGGCCGT

Product: octaprenyl diphosphate synthase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNIEQITELTAQDMAEVNTAIREQLNSEVALINQLGHYIISGGGKRIRPMIAVLAARALHYQGKQHVTVAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTALESLRVLELMSEAVNIIAEGEVLQLMNCNDPDITI
DNYMRVIYSKTARLFEAASQSSAILAGADACQEAALRDYGRYLGTAFQLIDDLLDYGADGKTLGKNTGDDLNEGKPTLPL
LHAMRNGTPEQAALIRQAIEQGNGRHLLETVLKTMHQCDSLGYTRRCAEEEADKAIACLDCLSPSPYRDALESLAHVAVQ
RDS

Sequences:

>Translated_323_residues
MNIEQITELTAQDMAEVNTAIREQLNSEVALINQLGHYIISGGGKRIRPMIAVLAARALHYQGKQHVTVAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTALESLRVLELMSEAVNIIAEGEVLQLMNCNDPDITI
DNYMRVIYSKTARLFEAASQSSAILAGADACQEAALRDYGRYLGTAFQLIDDLLDYGADGKTLGKNTGDDLNEGKPTLPL
LHAMRNGTPEQAALIRQAIEQGNGRHLLETVLKTMHQCDSLGYTRRCAEEEADKAIACLDCLSPSPYRDALESLAHVAVQ
RDS
>Mature_323_residues
MNIEQITELTAQDMAEVNTAIREQLNSEVALINQLGHYIISGGGKRIRPMIAVLAARALHYQGKQHVTVAALIEFIHTAT
LLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAFQMMTALESLRVLELMSEAVNIIAEGEVLQLMNCNDPDITI
DNYMRVIYSKTARLFEAASQSSAILAGADACQEAALRDYGRYLGTAFQLIDDLLDYGADGKTLGKNTGDDLNEGKPTLPL
LHAMRNGTPEQAALIRQAIEQGNGRHLLETVLKTMHQCDSLGYTRRCAEEEADKAIACLDCLSPSPYRDALESLAHVAVQ
RDS

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=322, Percent_Identity=31.3664596273292, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI4758430, Length=278, Percent_Identity=23.7410071942446, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI83700220, Length=278, Percent_Identity=23.7410071942446, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=79.8136645962733, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI1786623, Length=253, Percent_Identity=30.8300395256917, Blast_Score=82, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI17505681, Length=316, Percent_Identity=31.3291139240506, Blast_Score=151, Evalue=4e-37,
Organism=Saccharomyces cerevisiae, GI6319475, Length=299, Percent_Identity=36.1204013377926, Blast_Score=154, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6325188, Length=276, Percent_Identity=23.1884057971014, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24651612, Length=298, Percent_Identity=31.8791946308725, Blast_Score=157, Evalue=7e-39,
Organism=Drosophila melanogaster, GI281365769, Length=284, Percent_Identity=23.5915492957746, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24660002, Length=284, Percent_Identity=23.5915492957746, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35282; Mature: 35282

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00723 POLYPRENYL_SYNTHET_1 ; PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEQITELTAQDMAEVNTAIREQLNSEVALINQLGHYIISGGGKRIRPMIAVLAARALH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
YQGKQHVTVAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
HCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
QMMTALESLRVLELMSEAVNIIAEGEVLQLMNCNDPDITIDNYMRVIYSKTARLFEAASQ
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
SSAILAGADACQEAALRDYGRYLGTAFQLIDDLLDYGADGKTLGKNTGDDLNEGKPTLPL
CCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHH
LHAMRNGTPEQAALIRQAIEQGNGRHLLETVLKTMHQCDSLGYTRRCAEEEADKAIACLD
HHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
CLSPSPYRDALESLAHVAVQRDS
HCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNIEQITELTAQDMAEVNTAIREQLNSEVALINQLGHYIISGGGKRIRPMIAVLAARALH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
YQGKQHVTVAALIEFIHTATLLHDDVVDESDMRRGKATANAAFGNAASVLVGDFIYTRAF
HCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHH
QMMTALESLRVLELMSEAVNIIAEGEVLQLMNCNDPDITIDNYMRVIYSKTARLFEAASQ
HHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCC
SSAILAGADACQEAALRDYGRYLGTAFQLIDDLLDYGADGKTLGKNTGDDLNEGKPTLPL
CCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHH
LHAMRNGTPEQAALIRQAIEQGNGRHLLETVLKTMHQCDSLGYTRRCAEEEADKAIACLD
HHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
CLSPSPYRDALESLAHVAVQRDS
HCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]