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The map label for this gene is aspA [H]

Identifier: 85058281

GI number: 85058281

Start: 549430

End: 550443

Strand: Reverse

Name: aspA [H]

Synonym: SG0303

Alternate gene names: 85058281

Gene position: 550443-549430 (Counterclockwise)

Preceding gene: 85058286

Following gene: 85058280

Centisome position: 13.2

GC content: 55.23

Gene sequence:

>1014_bases
ATGTCAACCCTCATCCGTATCGAAGAAGACCTGCTTGGCACCCGCGAAGTGCCGGCTGACGTCTATTATGGCATTCATAC
CCTGCGCGCCAGCGAGAATTTCTACATCAGCAACACACGTATCAGCGATATTCCCGAGCTGGTACGCGGCATGGTGATGA
TCAAAAAAGCCACCGCGCTGGCCAATAAAGATTTGAAGACGCTGCCGCGCGATATCGCGGATATCATCGCCCGCGCCTGC
GATGAGGTGCTGGTCCGTGGCCGCTGCATGGATCAGTTTCCGGTAGATGTATTTCAGGGCGGCGCCGGCACCTCGGTCAA
TATGAATACTAATGAAGTGCTGGCCAATATCGGCCTGGAGCTGATGGGCCATCAGAAAGGAGAATACCTATTCCTCAATC
CCAACAATCATTTAAACTTATGTCAGTCTACCAATGACGCTTATCCCACCAGGTTTCGCCTGGCGGTTTATCATGCGCTC
CAGCGCCTAGTGGAAGCGATCGATTACTTGGGGGCGGGCTTTGAGCAGAACACGCAGCAATTCGCCGGTATTTTGAAAAT
GGGCAGAACACAGCTGCAGGACGCGGTCCCCATGACGCTCGGACAGGAATTCCACGCGTTTAACGTATTACTCAAAGAGG
AAAACAAAAACCTGCTGCGCACCGCGGAATTGCTGCTGGAAGTGAATCTAGGAGCGACCGCCATCGGTACCCGGCTCAAT
ACCCCGGAGGGGTATCAGGCGCTGGCGGTGGAAAAACTGGCGCAGATTAGCGGCCTTCCCTGCGTGCCGGCAGAGGATTT
GATTGAGGCCACCTCCGATTGCGGCGCCTATGTCATGGTCCATAGCGCGTTGAAACGCCTGGCGGTGAAGCTATCTAAAA
TCTGCAACGACCTGAGGCTGCTGTCCTCAGGGCCGCGGGCCGGCCTCAACGAAATCAATCTGCCCCAACTGCAGGCGGGA
TCGTCCATCATGCCTGCTAAAGTCAACCCCGTACTGCCGGAAGTGGTGAATTAG

Upstream 100 bases:

>100_bases
AAAATAAAATCAACGTCAGTTCAAGACACTACAAATAGTAGATATTAACCCATTTTATTTTAACATTAATGATAAAGAAC
GACAATTACAAGTAAGACGC

Downstream 100 bases:

>100_bases
GTGTGCTTCAAGGTCATCGGCAACGATACCTGTATCACCATAGCGGCGGAAGCGGGTCAGTTGCAGTTGAACGTCATGGA
ACCGGTTATCGGCCAGGCGA

Product: putative aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSTLIRIEEDLLGTREVPADVYYGIHTLRASENFYISNTRISDIPELVRGMVMIKKATALANKDLKTLPRDIADIIARAC
DEVLVRGRCMDQFPVDVFQGGAGTSVNMNTNEVLANIGLELMGHQKGEYLFLNPNNHLNLCQSTNDAYPTRFRLAVYHAL
QRLVEAIDYLGAGFEQNTQQFAGILKMGRTQLQDAVPMTLGQEFHAFNVLLKEENKNLLRTAELLLEVNLGATAIGTRLN
TPEGYQALAVEKLAQISGLPCVPAEDLIEATSDCGAYVMVHSALKRLAVKLSKICNDLRLLSSGPRAGLNEINLPQLQAG
SSIMPAKVNPVLPEVVN

Sequences:

>Translated_337_residues
MSTLIRIEEDLLGTREVPADVYYGIHTLRASENFYISNTRISDIPELVRGMVMIKKATALANKDLKTLPRDIADIIARAC
DEVLVRGRCMDQFPVDVFQGGAGTSVNMNTNEVLANIGLELMGHQKGEYLFLNPNNHLNLCQSTNDAYPTRFRLAVYHAL
QRLVEAIDYLGAGFEQNTQQFAGILKMGRTQLQDAVPMTLGQEFHAFNVLLKEENKNLLRTAELLLEVNLGATAIGTRLN
TPEGYQALAVEKLAQISGLPCVPAEDLIEATSDCGAYVMVHSALKRLAVKLSKICNDLRLLSSGPRAGLNEINLPQLQAG
SSIMPAKVNPVLPEVVN
>Mature_336_residues
STLIRIEEDLLGTREVPADVYYGIHTLRASENFYISNTRISDIPELVRGMVMIKKATALANKDLKTLPRDIADIIARACD
EVLVRGRCMDQFPVDVFQGGAGTSVNMNTNEVLANIGLELMGHQKGEYLFLNPNNHLNLCQSTNDAYPTRFRLAVYHALQ
RLVEAIDYLGAGFEQNTQQFAGILKMGRTQLQDAVPMTLGQEFHAFNVLLKEENKNLLRTAELLLEVNLGATAIGTRLNT
PEGYQALAVEKLAQISGLPCVPAEDLIEATSDCGAYVMVHSALKRLAVKLSKICNDLRLLSSGPRAGLNEINLPQLQAGS
SIMPAKVNPVLPEVVN

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=335, Percent_Identity=39.4029850746269, Blast_Score=231, Evalue=1e-60,
Organism=Escherichia coli, GI87082375, Length=327, Percent_Identity=79.5107033639144, Blast_Score=545, Evalue=1e-156,
Organism=Escherichia coli, GI1787896, Length=333, Percent_Identity=41.4414414414414, Blast_Score=248, Evalue=5e-67,
Organism=Caenorhabditis elegans, GI17553882, Length=333, Percent_Identity=41.1411411411411, Blast_Score=250, Evalue=9e-67,
Organism=Caenorhabditis elegans, GI32565146, Length=198, Percent_Identity=41.9191919191919, Blast_Score=154, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6324993, Length=340, Percent_Identity=39.1176470588235, Blast_Score=226, Evalue=6e-60,
Organism=Drosophila melanogaster, GI78710009, Length=330, Percent_Identity=40.6060606060606, Blast_Score=243, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24640179, Length=331, Percent_Identity=40.1812688821752, Blast_Score=241, Evalue=5e-64,
Organism=Drosophila melanogaster, GI24640177, Length=331, Percent_Identity=40.1812688821752, Blast_Score=241, Evalue=6e-64,
Organism=Drosophila melanogaster, GI24662684, Length=334, Percent_Identity=40.7185628742515, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24583245, Length=339, Percent_Identity=35.3982300884956, Blast_Score=220, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 36973; Mature: 36842

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLIRIEEDLLGTREVPADVYYGIHTLRASENFYISNTRISDIPELVRGMVMIKKATAL
CCCEEEEHHHHCCCCCCCHHHHHHHHHEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
ANKDLKTLPRDIADIIARACDEVLVRGRCMDQFPVDVFQGGAGTSVNMNTNEVLANIGLE
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEECCCHHHHHHHHCHH
LMGHQKGEYLFLNPNNHLNLCQSTNDAYPTRFRLAVYHALQRLVEAIDYLGAGFEQNTQQ
HCCCCCCCEEEECCCCCEEHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
FAGILKMGRTQLQDAVPMTLGQEFHAFNVLLKEENKNLLRTAELLLEVNLGATAIGTRLN
HHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHCCCCC
TPEGYQALAVEKLAQISGLPCVPAEDLIEATSDCGAYVMVHSALKRLAVKLSKICNDLRL
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSSGPRAGLNEINLPQLQAGSSIMPAKVNPVLPEVVN
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCC
>Mature Secondary Structure 
STLIRIEEDLLGTREVPADVYYGIHTLRASENFYISNTRISDIPELVRGMVMIKKATAL
CCEEEEHHHHCCCCCCCHHHHHHHHHEECCCCEEEECCCHHHHHHHHHHHHHHHHHHHH
ANKDLKTLPRDIADIIARACDEVLVRGRCMDQFPVDVFQGGAGTSVNMNTNEVLANIGLE
HCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCEECCCHHHHHHHHCHH
LMGHQKGEYLFLNPNNHLNLCQSTNDAYPTRFRLAVYHALQRLVEAIDYLGAGFEQNTQQ
HCCCCCCCEEEECCCCCEEHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
FAGILKMGRTQLQDAVPMTLGQEFHAFNVLLKEENKNLLRTAELLLEVNLGATAIGTRLN
HHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHCCCCC
TPEGYQALAVEKLAQISGLPCVPAEDLIEATSDCGAYVMVHSALKRLAVKLSKICNDLRL
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LSSGPRAGLNEINLPQLQAGSSIMPAKVNPVLPEVVN
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]