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The map label for this gene is metC [H]

Identifier: 85058258

GI number: 85058258

Start: 503845

End: 505041

Strand: Reverse

Name: metC [H]

Synonym: SG0280

Alternate gene names: 85058258

Gene position: 505041-503845 (Counterclockwise)

Preceding gene: 85058261

Following gene: 85058257

Centisome position: 12.11

GC content: 55.56

Gene sequence:

>1197_bases
ATGACGTCGAAGAAATTAGAAACGGTGCTGATAAGCGCCGGCAGAAAACCTGCTTTCACGCAGGGTTCAGTGAACCCGAT
TATCCAGCGGGCATCCTCGCTGGTATTTGATTCCGTCCAGCACAAGAAAAAGGCCACGGCGGGACGCGCCAGCGGCGAGT
TGTATTACGGTCGGCGCGGTACGCTTACCCATTTTTCCCTCCAGCAAGCGATGGTGGAGCTGGAAGGCGGCGCCGGCTGC
GCGTTTTATCCGTGCGGTACCGCCGCGGTCAGCAACGCGATTTTGTCTTTCGTCTCGGCGGGCGATCATATTTTAGTCAC
CGGTTCGGCTTATGAGCCAACACAGGACTTTTGCCGGCAAATACTCGGCAAAATGAATATCACGACCACCTGGTTCGATC
CGCTCATTGGCGCGGATATCGGCGATCTGATTCAGGATAATACGCGGGTGGTGATGCTGGAATCCCCAGGATCCATTACC
ATGGAAGTACAAGACATTCCCGCCATTGTGCGTGCGGTGCGCGCCAAAAATCCCGATATCATCATTATGTTGGATAATAC
CTGGGCGGCAGGCGTACTGCTTAAGGCGCTGGATCTGGGGGTTGATATTTCTCTTCAGGCCGGCACCAAATATCTTATCG
GCCATTCCGACGCCATGATCGGCACCGCCGTGGCCAACGCGCGCTGCTGGGACCAATTACGCGAGCAATCCTATCTGATG
GGCCAGATGGTTGACGCCGATACCGCTTATATGGCCGAGCGAGGGCTGCGTACCCTTGGCATACGGTTAAAACAGCATGA
AGAAAATGGCCTGCGCGTGGCACGCTGGCTGACGCAACGCCCGGAAGTGGCGGTCGTCAATCATCCCGCGCTGCCGGGGT
GCAAAGGCCACGAAGCCTTTCTTCGGGACTTTACCGGCGCCAGCGGGTTGTTTTCCTTCGTCCTCAAACAACGATTAAGC
GATGATCAACTGGCACATTATCTGGATCATTTTACACACTTTAGTATTTCTTACTCTTGGGGTGGCTTTGAATCACTGAT
AATGGCGAATCAGCCGGAAGAACTGGCGGCTATCCGCCCCGCCGGCGGCGTCGATTTCACCGGCACGCTGGTACGACTGC
ATATCGGCCTGGAGAGCTGCGAGGATTTGATCGACGATTTAGCCTCAGGCTTCGCCCGCATCGCGTCATCTAAGTGA

Upstream 100 bases:

>100_bases
AAACGTCTGCCCCGCGCCCGACATAAGTGATAACCTGAATCGGATGATCTCCGCCGCCACGTACGGCATAAAATTTCTGG
CAAAAGGCAAAGCGCGCATA

Downstream 100 bases:

>100_bases
TGAAATCGCGTTTATACGCCGATTCATCGCCGGTTGACGCTTTGCTGACTAAAATAGATTAAAAAAATGTTTGGTTGGGT
GATGGATCAAGGACTGCAAT

Product: cystathionine beta-lyase

Products: NA

Alternate protein names: CBL; Beta-cystathionase; Cysteine lyase [H]

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTSKKLETVLISAGRKPAFTQGSVNPIIQRASSLVFDSVQHKKKATAGRASGELYYGRRGTLTHFSLQQAMVELEGGAGC
AFYPCGTAAVSNAILSFVSAGDHILVTGSAYEPTQDFCRQILGKMNITTTWFDPLIGADIGDLIQDNTRVVMLESPGSIT
MEVQDIPAIVRAVRAKNPDIIIMLDNTWAAGVLLKALDLGVDISLQAGTKYLIGHSDAMIGTAVANARCWDQLREQSYLM
GQMVDADTAYMAERGLRTLGIRLKQHEENGLRVARWLTQRPEVAVVNHPALPGCKGHEAFLRDFTGASGLFSFVLKQRLS
DDQLAHYLDHFTHFSISYSWGGFESLIMANQPEELAAIRPAGGVDFTGTLVRLHIGLESCEDLIDDLASGFARIASSK

Sequences:

>Translated_398_residues
MTSKKLETVLISAGRKPAFTQGSVNPIIQRASSLVFDSVQHKKKATAGRASGELYYGRRGTLTHFSLQQAMVELEGGAGC
AFYPCGTAAVSNAILSFVSAGDHILVTGSAYEPTQDFCRQILGKMNITTTWFDPLIGADIGDLIQDNTRVVMLESPGSIT
MEVQDIPAIVRAVRAKNPDIIIMLDNTWAAGVLLKALDLGVDISLQAGTKYLIGHSDAMIGTAVANARCWDQLREQSYLM
GQMVDADTAYMAERGLRTLGIRLKQHEENGLRVARWLTQRPEVAVVNHPALPGCKGHEAFLRDFTGASGLFSFVLKQRLS
DDQLAHYLDHFTHFSISYSWGGFESLIMANQPEELAAIRPAGGVDFTGTLVRLHIGLESCEDLIDDLASGFARIASSK
>Mature_397_residues
TSKKLETVLISAGRKPAFTQGSVNPIIQRASSLVFDSVQHKKKATAGRASGELYYGRRGTLTHFSLQQAMVELEGGAGCA
FYPCGTAAVSNAILSFVSAGDHILVTGSAYEPTQDFCRQILGKMNITTTWFDPLIGADIGDLIQDNTRVVMLESPGSITM
EVQDIPAIVRAVRAKNPDIIIMLDNTWAAGVLLKALDLGVDISLQAGTKYLIGHSDAMIGTAVANARCWDQLREQSYLMG
QMVDADTAYMAERGLRTLGIRLKQHEENGLRVARWLTQRPEVAVVNHPALPGCKGHEAFLRDFTGASGLFSFVLKQRLSD
DQLAHYLDHFTHFSISYSWGGFESLIMANQPEELAAIRPAGGVDFTGTLVRLHIGLESCEDLIDDLASGFARIASSK

Specific function: Methionine biosynthesis; third step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=383, Percent_Identity=30.8093994778068, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI299473759, Length=383, Percent_Identity=28.4595300261097, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI25453487, Length=383, Percent_Identity=27.6762402088773, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1789383, Length=394, Percent_Identity=70.0507614213198, Blast_Score=607, Evalue=1e-175,
Organism=Escherichia coli, GI1790375, Length=395, Percent_Identity=28.8607594936709, Blast_Score=141, Evalue=6e-35,
Organism=Caenorhabditis elegans, GI17560056, Length=334, Percent_Identity=32.3353293413174, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17538055, Length=384, Percent_Identity=29.9479166666667, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71980708, Length=401, Percent_Identity=28.6783042394015, Blast_Score=154, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6321170, Length=337, Percent_Identity=42.7299703264095, Blast_Score=261, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6319307, Length=360, Percent_Identity=26.9444444444444, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321254, Length=264, Percent_Identity=29.9242424242424, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321904, Length=252, Percent_Identity=30.1587301587302, Blast_Score=92, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323333, Length=420, Percent_Identity=24.5238095238095, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19922554, Length=337, Percent_Identity=32.9376854599407, Blast_Score=176, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006233
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.8 [H]

Molecular weight: Translated: 43235; Mature: 43104

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSKKLETVLISAGRKPAFTQGSVNPIIQRASSLVFDSVQHKKKATAGRASGELYYGRRG
CCCHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCC
TLTHFSLQQAMVELEGGAGCAFYPCGTAAVSNAILSFVSAGDHILVTGSAYEPTQDFCRQ
CCHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
ILGKMNITTTWFDPLIGADIGDLIQDNTRVVMLESPGSITMEVQDIPAIVRAVRAKNPDI
HHCCCCCEEEHHHHHHCCCHHHHHCCCCEEEEEECCCCEEEEEHHHHHHHHHHHCCCCCE
IIMLDNTWAAGVLLKALDLGVDISLQAGTKYLIGHSDAMIGTAVANARCWDQLREQSYLM
EEEECCCHHHHHHHHHHHCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
GQMVDADTAYMAERGLRTLGIRLKQHEENGLRVARWLTQRPEVAVVNHPALPGCKGHEAF
HHHCCCHHHHHHHCCHHHHCCEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHH
LRDFTGASGLFSFVLKQRLSDDQLAHYLDHFTHFSISYSWGGFESLIMANQPEELAAIRP
HHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEECCCCHHHHHCCCCCHHHHEECC
AGGVDFTGTLVRLHIGLESCEDLIDDLASGFARIASSK
CCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSKKLETVLISAGRKPAFTQGSVNPIIQRASSLVFDSVQHKKKATAGRASGELYYGRRG
CCHHHHHHHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCC
TLTHFSLQQAMVELEGGAGCAFYPCGTAAVSNAILSFVSAGDHILVTGSAYEPTQDFCRQ
CCHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
ILGKMNITTTWFDPLIGADIGDLIQDNTRVVMLESPGSITMEVQDIPAIVRAVRAKNPDI
HHCCCCCEEEHHHHHHCCCHHHHHCCCCEEEEEECCCCEEEEEHHHHHHHHHHHCCCCCE
IIMLDNTWAAGVLLKALDLGVDISLQAGTKYLIGHSDAMIGTAVANARCWDQLREQSYLM
EEEECCCHHHHHHHHHHHCCCEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
GQMVDADTAYMAERGLRTLGIRLKQHEENGLRVARWLTQRPEVAVVNHPALPGCKGHEAF
HHHCCCHHHHHHHCCHHHHCCEEEECCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCHHH
LRDFTGASGLFSFVLKQRLSDDQLAHYLDHFTHFSISYSWGGFESLIMANQPEELAAIRP
HHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHEEEEEECCCCHHHHHCCCCCHHHHEECC
AGGVDFTGTLVRLHIGLESCEDLIDDLASGFARIASSK
CCCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2659970; 11677609 [H]