BacMap

Definition	Sodalis glossinidius str. 'morsitans', complete genome.
Accession	NC_007712
Length	4,171,146

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The map label for this gene is dkgA [H]

Identifier: 85058256

GI number: 85058256

Start: 502324

End: 502881

Strand: Reverse

Name: dkgA [H]

Synonym: SG0278

Alternate gene names: 85058256

Gene position: 502881-502324 (Counterclockwise)

Preceding gene: 85058257

Following gene: 85058252

Centisome position: 12.06

GC content: 52.69

Gene sequence:

>558_bases
ATGACAACGCAACCCACGGTCACATTCCATGATGGACACACCATGCCACAATTGGGTTTGGGTGTCTGGAAAGCCACTAA
CCAGGAAGTCGTCAGTGCAGTTGAGGCGGCGCTGGATGCCGATTATCGCTCCATTGATACCGCGGCGATGTATAAAAATG
AAGAGGGTGTCGGTCAGGCGCTGCAGGGTGCAACGATGGCGCGGGAAGATCTGTTTATCACCACCAAACTATGGAACGGT
GATCAAACCAGAGTGCCCAAGGCTATTGAGCAAAGTCTGACCAAGCTGCGGCTGGATTATGTCGATCTTTATCTGATGCA
TTGGCCGGTGCCCAGCCAGGATAACTATGCACAAGCCTGGCGCGAGATGATGAGGCTTAAGGAGGAAGGGCTGGCAAAGA
GCATTGGGGTGTGTAATTTTGAGCCGCATCATCTTCAGCGCCTGCTGGATGAAATCGGGGTGGTGCCGGTAATCAATCAG
ATCGAGCTGCATCCGCTGTTACAACAACAGGCATTGCGGACCTTTGATGCCCGCCACCAGATTTTGACCGAATCTTAG

Upstream 100 bases:

>100_bases
GAAATCCGCTCCATAACTGGCTTTCGGCGTAACCTATTCCCTTACTCATAACCTTCGCCTCCCCGCGTGGGTGTGGCGGT
TGGACAAAAAAGGAGAAATC

Downstream 100 bases:

>100_bases
AGCCCTCTGGCGCAGGGGGGCAAAGGGGTATTTGATCAGGCGATCATCCGTGAACTTGCGGAAAAATATAGCAAAACGCC
GGCGCGGATCCGCGCCAACT

Product: putative 2,5-diketo-D-gluconate reductase A

Products: NA

Alternate protein names: 2,5-DKG reductase A; 2,5-DKGR A; 25DKGR-A; AKR5C [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MTTQPTVTFHDGHTMPQLGLGVWKATNQEVVSAVEAALDADYRSIDTAAMYKNEEGVGQALQGATMAREDLFITTKLWNG
DQTRVPKAIEQSLTKLRLDYVDLYLMHWPVPSQDNYAQAWREMMRLKEEGLAKSIGVCNFEPHHLQRLLDEIGVVPVINQ
IELHPLLQQQALRTFDARHQILTES

Sequences:

>Translated_185_residues
MTTQPTVTFHDGHTMPQLGLGVWKATNQEVVSAVEAALDADYRSIDTAAMYKNEEGVGQALQGATMAREDLFITTKLWNG
DQTRVPKAIEQSLTKLRLDYVDLYLMHWPVPSQDNYAQAWREMMRLKEEGLAKSIGVCNFEPHHLQRLLDEIGVVPVINQ
IELHPLLQQQALRTFDARHQILTES
>Mature_184_residues
TTQPTVTFHDGHTMPQLGLGVWKATNQEVVSAVEAALDADYRSIDTAAMYKNEEGVGQALQGATMAREDLFITTKLWNGD
QTRVPKAIEQSLTKLRLDYVDLYLMHWPVPSQDNYAQAWREMMRLKEEGLAKSIGVCNFEPHHLQRLLDEIGVVPVINQI
ELHPLLQQQALRTFDARHQILTES

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI223468663, Length=184, Percent_Identity=40.7608695652174, Blast_Score=132, Evalue=2e-31,
Organism=Homo sapiens, GI310109922, Length=188, Percent_Identity=40.4255319148936, Blast_Score=131, Evalue=3e-31,
Organism=Homo sapiens, GI310109920, Length=206, Percent_Identity=38.3495145631068, Blast_Score=131, Evalue=4e-31,
Organism=Homo sapiens, GI5453543, Length=206, Percent_Identity=37.8640776699029, Blast_Score=130, Evalue=8e-31,
Organism=Homo sapiens, GI24497585, Length=205, Percent_Identity=38.0487804878049, Blast_Score=129, Evalue=1e-30,
Organism=Homo sapiens, GI45446745, Length=206, Percent_Identity=37.378640776699, Blast_Score=129, Evalue=1e-30,
Organism=Homo sapiens, GI4503285, Length=206, Percent_Identity=37.378640776699, Blast_Score=129, Evalue=1e-30,
Organism=Homo sapiens, GI24497577, Length=192, Percent_Identity=36.9791666666667, Blast_Score=128, Evalue=3e-30,
Organism=Homo sapiens, GI5174391, Length=192, Percent_Identity=36.9791666666667, Blast_Score=128, Evalue=3e-30,
Organism=Homo sapiens, GI24497583, Length=206, Percent_Identity=36.8932038834951, Blast_Score=123, Evalue=7e-29,
Organism=Homo sapiens, GI93277124, Length=206, Percent_Identity=35.9223300970874, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI291291012, Length=170, Percent_Identity=39.4117647058824, Blast_Score=117, Evalue=6e-27,
Organism=Homo sapiens, GI300116273, Length=206, Percent_Identity=33.0097087378641, Blast_Score=109, Evalue=1e-24,
Organism=Homo sapiens, GI4502049, Length=190, Percent_Identity=39.4736842105263, Blast_Score=109, Evalue=1e-24,
Organism=Homo sapiens, GI5174695, Length=206, Percent_Identity=33.0097087378641, Blast_Score=108, Evalue=2e-24,
Organism=Homo sapiens, GI310109926, Length=119, Percent_Identity=42.0168067226891, Blast_Score=93, Evalue=1e-19,
Organism=Homo sapiens, GI207028673, Length=119, Percent_Identity=40.3361344537815, Blast_Score=92, Evalue=3e-19,
Organism=Homo sapiens, GI300116271, Length=114, Percent_Identity=36.8421052631579, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI87082198, Length=185, Percent_Identity=62.7027027027027, Blast_Score=254, Evalue=4e-69,
Organism=Escherichia coli, GI1786400, Length=171, Percent_Identity=36.8421052631579, Blast_Score=122, Evalue=1e-29,
Organism=Escherichia coli, GI1788081, Length=155, Percent_Identity=30.9677419354839, Blast_Score=70, Evalue=8e-14,
Organism=Escherichia coli, GI48994888, Length=165, Percent_Identity=29.0909090909091, Blast_Score=65, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17550248, Length=195, Percent_Identity=37.4358974358974, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17566692, Length=193, Percent_Identity=38.860103626943, Blast_Score=125, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17562292, Length=196, Percent_Identity=36.2244897959184, Blast_Score=124, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17538386, Length=192, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17552492, Length=184, Percent_Identity=35.3260869565217, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17564128, Length=196, Percent_Identity=35.2040816326531, Blast_Score=117, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17561300, Length=195, Percent_Identity=32.8205128205128, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17537077, Length=202, Percent_Identity=33.6633663366337, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17537075, Length=202, Percent_Identity=33.6633663366337, Blast_Score=114, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI17561298, Length=193, Percent_Identity=31.6062176165803, Blast_Score=108, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI71998625, Length=186, Percent_Identity=33.3333333333333, Blast_Score=107, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17537079, Length=204, Percent_Identity=27.4509803921569, Blast_Score=97, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17550246, Length=77, Percent_Identity=45.4545454545455, Blast_Score=74, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320576, Length=212, Percent_Identity=38.2075471698113, Blast_Score=150, Evalue=1e-37,
Organism=Saccharomyces cerevisiae, GI6324694, Length=207, Percent_Identity=38.1642512077295, Blast_Score=145, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6319625, Length=217, Percent_Identity=35.0230414746544, Blast_Score=118, Evalue=7e-28,
Organism=Saccharomyces cerevisiae, GI6321896, Length=209, Percent_Identity=33.0143540669856, Blast_Score=112, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6322556, Length=186, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6320079, Length=151, Percent_Identity=37.0860927152318, Blast_Score=91, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20129731, Length=199, Percent_Identity=39.1959798994975, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24663317, Length=207, Percent_Identity=37.6811594202899, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24644950, Length=207, Percent_Identity=38.6473429951691, Blast_Score=136, Evalue=9e-33,
Organism=Drosophila melanogaster, GI24662781, Length=195, Percent_Identity=40.5128205128205, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24662789, Length=199, Percent_Identity=38.1909547738693, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24657054, Length=200, Percent_Identity=39, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI21356425, Length=204, Percent_Identity=39.2156862745098, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI221378297, Length=227, Percent_Identity=35.2422907488987, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24662785, Length=201, Percent_Identity=39.3034825870647, Blast_Score=127, Evalue=5e-30,
Organism=Drosophila melanogaster, GI45553081, Length=206, Percent_Identity=35.4368932038835, Blast_Score=117, Evalue=5e-27,
Organism=Drosophila melanogaster, GI281366140, Length=165, Percent_Identity=35.1515151515151, Blast_Score=89, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 20996; Mature: 20865

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQPTVTFHDGHTMPQLGLGVWKATNQEVVSAVEAALDADYRSIDTAAMYKNEEGVGQA
CCCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHH
LQGATMAREDLFITTKLWNGDQTRVPKAIEQSLTKLRLDYVDLYLMHWPVPSQDNYAQAW
HHHHHHHHCCEEEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHH
REMMRLKEEGLAKSIGVCNFEPHHLQRLLDEIGVVPVINQIELHPLLQQQALRTFDARHQ
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ILTES
HHCCC
>Mature Secondary Structure 
TTQPTVTFHDGHTMPQLGLGVWKATNQEVVSAVEAALDADYRSIDTAAMYKNEEGVGQA
CCCCEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHH
LQGATMAREDLFITTKLWNGDQTRVPKAIEQSLTKLRLDYVDLYLMHWPVPSQDNYAQAW
HHHHHHHHCCEEEEEEEECCCHHHCCHHHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHH
REMMRLKEEGLAKSIGVCNFEPHHLQRLLDEIGVVPVINQIELHPLLQQQALRTFDARHQ
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ILTES
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]