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The map label for this gene is cca

Identifier: 85058236

GI number: 85058236

Start: 473230

End: 474477

Strand: Reverse

Name: cca

Synonym: SG0258

Alternate gene names: 85058236

Gene position: 474477-473230 (Counterclockwise)

Preceding gene: 85058237

Following gene: 85058233

Centisome position: 11.38

GC content: 62.58

Gene sequence:

>1248_bases
GTGAAAAAATATCTGGTCGGCGGCGCCGTGCGTGACGGTCTGTTACAGCTCCCCGTCAAGGAACGGGATTGGGTGGTGGT
CGGCGCAACGCCGCAGGAGATGCTCGCGCAAGGTTATCAGCATGTCGGCAAAGATTTTCCGGTTTTTCTGCATCCCGATA
GCCGCGAGGAGTATGCGCTGGCCCGTACGGAGCGCAAATCCGGTCAGGGCTATACCGGCTTCATCTGCTACGCCTCGCCG
GAAGTCACGCTGGAAGAAGATCTGCGCCGCCGCGATCTCACCATTAATGCCATCGCCCGCGACGACGAGGGTAACCTGAT
TGATCCCTATCAGGGACAGCAGGACATCCGCCAACGCTGGTTGCGCCATGTCTCCGACGCCTTCGGCGAAGATCCGCTGC
GCGTACTGCGGGTCGCCCGTTTTGCCGCGCGTTTCGCCCATTTGAATTTCCGCATCGCGCCGGAAACGATGGCGTTGATG
CAGCAGATGACCGACGAGCTGCCGCTGCTGGCGCCTGAGCGTGTCTGGAAAGAGACGGAGCGGACGCTGGCAACCCGCAA
TCCGCAGGTCTATTTTCAGGTCCTGCGCGACTGCGGCGCGTTAAAAGCGCTATTTCCGGAGGTGGACGCGCTATTTGGCG
TCCCGGCGCCGGCGAAATGGCACCCGGAAATCGATACCGGCATCCACACTCTGATGACGGTGTCGATGGCGGCCCGGTTA
ACGGACGATATCGCGGTACGCTTCGCTACACTTTGCCACGATGTGGGCAAGGCGCTCACGCCGCGCGAGCTGTGGCCCAG
CCACCACGGCCACGGCCCGGCGGGGGTGAAGGTGGTGGAAGCGCTGTGCCAGCGCCTGAAGGTGCCCAATCCGCTGCGCG
ATCTGGCCACGATCGTGGCCCGCTACCATGATCTTTTGCACGGTGCCCAGAGCCTGACGCCAAAGACACTGATAAAACTG
TTCAGCGCCATTGATGTCTGGCGCCGACCGGCGCGCCTGGAGCAGATGATCCTCGCCAGCGAAGCCGACGCACGTGGCCG
TACCGGGTTTGAGAACCATGCCTATCCGCAGGGGGATTTTCTGCGCGACGCGTTCCGCGTAGCCAGTTCAGTCAGCGCGC
GAGATGTGATGGCGGCGGGATTCAGCGGCGCGCAGATCGGCGAGGAGCTCAACCGGCGACGCCAGCAGGCGCTGGCGAGC
TGGAAGCGACAGCAAGAGAATAGCGATACCGAGACCATTCAGGACTGA

Upstream 100 bases:

>100_bases
GCCGCCCCCGACACGGGTGGCGATGAATCTCGTGCGAGATACGGCTACACTCAATGGACGGTACCGATAAAGGCAACACC
GGCTTTGCCAGGAGAGAATT

Downstream 100 bases:

>100_bases
CCCTTACTGCACCGCGTATACTGCGTCAGATACTGCATATGCCGTCAGACATGCCGTAACCATGGCGCCGGGCGGAGGCG
CAGCAAAAACAGCATTAGCC

Product: multifunctional tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase/phosphatase

Products: NA

Alternate protein names: CCA-adding enzyme; tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl-transferase; tRNA nucleotidyltransferase; tRNA-NT; 2'-nucleotidase; 2',3'-cyclic phosphodiesterase; Phosphatase

Number of amino acids: Translated: 415; Mature: 415

Protein sequence:

>415_residues
MKKYLVGGAVRDGLLQLPVKERDWVVVGATPQEMLAQGYQHVGKDFPVFLHPDSREEYALARTERKSGQGYTGFICYASP
EVTLEEDLRRRDLTINAIARDDEGNLIDPYQGQQDIRQRWLRHVSDAFGEDPLRVLRVARFAARFAHLNFRIAPETMALM
QQMTDELPLLAPERVWKETERTLATRNPQVYFQVLRDCGALKALFPEVDALFGVPAPAKWHPEIDTGIHTLMTVSMAARL
TDDIAVRFATLCHDVGKALTPRELWPSHHGHGPAGVKVVEALCQRLKVPNPLRDLATIVARYHDLLHGAQSLTPKTLIKL
FSAIDVWRRPARLEQMILASEADARGRTGFENHAYPQGDFLRDAFRVASSVSARDVMAAGFSGAQIGEELNRRRQQALAS
WKRQQENSDTETIQD

Sequences:

>Translated_415_residues
MKKYLVGGAVRDGLLQLPVKERDWVVVGATPQEMLAQGYQHVGKDFPVFLHPDSREEYALARTERKSGQGYTGFICYASP
EVTLEEDLRRRDLTINAIARDDEGNLIDPYQGQQDIRQRWLRHVSDAFGEDPLRVLRVARFAARFAHLNFRIAPETMALM
QQMTDELPLLAPERVWKETERTLATRNPQVYFQVLRDCGALKALFPEVDALFGVPAPAKWHPEIDTGIHTLMTVSMAARL
TDDIAVRFATLCHDVGKALTPRELWPSHHGHGPAGVKVVEALCQRLKVPNPLRDLATIVARYHDLLHGAQSLTPKTLIKL
FSAIDVWRRPARLEQMILASEADARGRTGFENHAYPQGDFLRDAFRVASSVSARDVMAAGFSGAQIGEELNRRRQQALAS
WKRQQENSDTETIQD
>Mature_415_residues
MKKYLVGGAVRDGLLQLPVKERDWVVVGATPQEMLAQGYQHVGKDFPVFLHPDSREEYALARTERKSGQGYTGFICYASP
EVTLEEDLRRRDLTINAIARDDEGNLIDPYQGQQDIRQRWLRHVSDAFGEDPLRVLRVARFAARFAHLNFRIAPETMALM
QQMTDELPLLAPERVWKETERTLATRNPQVYFQVLRDCGALKALFPEVDALFGVPAPAKWHPEIDTGIHTLMTVSMAARL
TDDIAVRFATLCHDVGKALTPRELWPSHHGHGPAGVKVVEALCQRLKVPNPLRDLATIVARYHDLLHGAQSLTPKTLIKL
FSAIDVWRRPARLEQMILASEADARGRTGFENHAYPQGDFLRDAFRVASSVSARDVMAAGFSGAQIGEELNRRRQQALAS
WKRQQENSDTETIQD

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1789436, Length=407, Percent_Identity=72.2358722358722, Blast_Score=613, Evalue=1e-177,
Organism=Escherichia coli, GI87081691, Length=316, Percent_Identity=26.2658227848101, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CCA_SODGM (Q2NWE2)

Other databases:

- EMBL:   AP008232
- RefSeq:   YP_453938.1
- ProteinModelPortal:   Q2NWE2
- SMR:   Q2NWE2
- STRING:   Q2NWE2
- GeneID:   3867898
- GenomeReviews:   AP008232_GR
- KEGG:   sgl:SG0258
- NMPDR:   fig|343509.6.peg.614
- eggNOG:   COG0617
- HOGENOM:   HBG635063
- OMA:   GHGQKGL
- PhylomeDB:   Q2NWE2
- ProtClustDB:   PRK10885
- BioCyc:   SGLO343509:SG0258-MONOMER
- HAMAP:   MF_01261
- InterPro:   IPR012006
- InterPro:   IPR003607
- InterPro:   IPR002646
- PIRSF:   PIRSF000813
- SMART:   SM00471

Pfam domain/function: PF01743 PolyA_pol

EC number: =2.7.7.21; =2.7.7.25

Molecular weight: Translated: 46802; Mature: 46802

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: NA

Important sites: BINDING 8-8 BINDING 11-11 BINDING 91-91 BINDING 137-137 BINDING 140-140

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKYLVGGAVRDGLLQLPVKERDWVVVGATPQEMLAQGYQHVGKDFPVFLHPDSREEYAL
CCCCCCCHHHHCCHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCHHHH
ARTERKSGQGYTGFICYASPEVTLEEDLRRRDLTINAIARDDEGNLIDPYQGQQDIRQRW
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHH
LRHVSDAFGEDPLRVLRVARFAARFAHLNFRIAPETMALMQQMTDELPLLAPERVWKETE
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHCCCCCCHHHHHHHHH
RTLATRNPQVYFQVLRDCGALKALFPEVDALFGVPAPAKWHPEIDTGIHTLMTVSMAARL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
TDDIAVRFATLCHDVGKALTPRELWPSHHGHGPAGVKVVEALCQRLKVPNPLRDLATIVA
HHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
RYHDLLHGAQSLTPKTLIKLFSAIDVWRRPARLEQMILASEADARGRTGFENHAYPQGDF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
LRDAFRVASSVSARDVMAAGFSGAQIGEELNRRRQQALASWKRQQENSDTETIQD
HHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC
>Mature Secondary Structure
MKKYLVGGAVRDGLLQLPVKERDWVVVGATPQEMLAQGYQHVGKDFPVFLHPDSREEYAL
CCCCCCCHHHHCCHHCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCHHHH
ARTERKSGQGYTGFICYASPEVTLEEDLRRRDLTINAIARDDEGNLIDPYQGQQDIRQRW
HHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCEEEEEEECCCCCCCCCCCCHHHHHHHH
LRHVSDAFGEDPLRVLRVARFAARFAHLNFRIAPETMALMQQMTDELPLLAPERVWKETE
HHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHHCCCCCCHHHHHHHHH
RTLATRNPQVYFQVLRDCGALKALFPEVDALFGVPAPAKWHPEIDTGIHTLMTVSMAARL
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
TDDIAVRFATLCHDVGKALTPRELWPSHHGHGPAGVKVVEALCQRLKVPNPLRDLATIVA
HHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHH
RYHDLLHGAQSLTPKTLIKLFSAIDVWRRPARLEQMILASEADARGRTGFENHAYPQGDF
HHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
LRDAFRVASSVSARDVMAAGFSGAQIGEELNRRRQQALASWKRQQENSDTETIQD
HHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA