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The map label for this gene is dnaG

Identifier: 85058230

GI number: 85058230

Start: 467454

End: 469202

Strand: Reverse

Name: dnaG

Synonym: SG0252

Alternate gene names: 85058230

Gene position: 469202-467454 (Counterclockwise)

Preceding gene: 85058231

Following gene: 85058229

Centisome position: 11.25

GC content: 57.23

Gene sequence:

>1749_bases
ATGGCTGGACGAATCCCACGTGTCTTTATCAATGACTTGTTGGCGCGAACCGACATCGTCGATCTGATCGATGCGCGGGT
AAAGCTCAAAAAGCAGGGCAAGAATTTTCACGCCTGCTGTCCATTCCATCATGAGAAAACCCCGTCGTTTACCGTGAACG
GCGAAAAGCAGTTTTACCATTGTTTCGGCTGTGGCACCCATGGCAACGCCATCGATTTCCTGATGAATTACGACCGGCTG
GAGTTCGTCGAATCCATCGAAGAGCTTGCCGCCCAGCACGGCCTGGACGTGCCCTATGAAACGGGCACCGGCCCGAACCA
GCAGAAGCGCCACCAGCGACAAAGCCTGTACCAGCTGATGGACAAACTCAGCGGCTTCTATCAACAGGCGCTACAGGCGC
AGACGGCATCGGATGCGCGCCACTATCTGCAACAGCGCGGGCTGAGCGAAGCGGTCATCAACCATTTTGCCATCGGTTTC
GCGCCGCCGGGCTGGGACAACGTCGATAAGCGTTTTGGCCGTTCGGCGGAGGATCGCGCCTCGCTCAACGATGCCGGCAT
GCTGGTTGTGAATGACAACGGCCGCACCTACGACCGTTTTCGCGATCGCATCATGTTTCCCATCCGCGACAAACGCGGGA
GGGTTATCGCTTTCGGTGGACGGATATTGGGCGAGGGTCAGCCGAAATACCTGAACTCGCCAGAAACCGATATTTTTCAT
AAAGGTCGCCAGCTGTACGGCCTGTATGAGGCCCAGCGGCAGCATCCGGAATTATCCCGGCTACTCGTGGTCGAAGGGTA
TATGGACGTCGTGGCGCTGGCACAGTTCGGTATCGACTACGCGGTGGCTTCGTTAGGCACCTCGACCACCGCGGAGCATA
TCCAACTGCTTTATCGCGCCACCGATCGGGTGATCTGCTGCTATGACGGCGACCGCGCTGGACGTGAAGCGGCCTGGCGC
GCGCTGGAAAACGCGCTGCCCTACTTGACCGATGGCCGCCAGCTACGCTTCATGTTTCTGCCGGACGGTGAGGATCCCGA
TACGCTGGTGCGCAAAATCGGCAAAGAGGCATTTGAAAAGTACATCGAACAGGCGCAGCCGCTGTCGACGTTTTTATTTG
AAACGCTGATGCCACAGGTGGACTTGAGCAGCCCCGACGGTCGTGCCAAGTTAAGCACGCTGGCGCTGCCGTTGATAGGC
CAGGTACCAGGCGAGACATTGCGTCTCTACCTGCGCCAGCAGTTGGGCAATAAACTCGGGATCCTTGACGACAGTCAACT
GGAGAAACTACTGCCCAAGGCGGCCCTTAAGGCAAATATTTCACCACAGTCGCGGATAAAACGCACAACCATGCGTATAC
TGATAGGATTGCTGGTGCAGCATCCGCGGCTTTCCGCCCTGGTACCTACGGTGCAGGGGCTGGAGCAGGCAATTCAGCCG
GGCGTGCCGTTGTTTGTTGAATTAGTGCAGACCTGCCAGGCCCAACCGGGGTTAACCACCGGCCAGTTGCTGGAGCATTA
TCGGGAGAATAAATCTTACTCCCAGCTTGAAACCCTGGCGACCTGGAACCACATGATCATAGATGAGATGATCGAGGCGA
CATTTGTCGATACCCTGGCGAGTCTTTACGACTCCATACTTGAACACCGCCAGGAAGCGCTTATCGCCCGCGACCGGACC
CACGGCCTGACGGCCGAAGAACGCCAGGAGCTCTGGTCGCTGAATCAGGCGCTGGCGAAAAAAACCTGA

Upstream 100 bases:

>100_bases
GTGATTAATATCCGCAGACTTAGCTGTTGCATACGAAGGCCGTGCCATCCGGAAGGATTGCGCGGCCTGTTGTCGTTCAA
GTGCTTACCTGACAGGGCTT

Downstream 100 bases:

>100_bases
ATGACGAGGCTTAATTGCCTATATCTGGCAGGGCAAGACCCTGCGCAATGCCGTATTGGGCTGCGGCGCGAAACAAAGCT
CAGATCTCTTGTTGGTGATG

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHHEKTPSFTVNGEKQFYHCFGCGTHGNAIDFLMNYDRL
EFVESIEELAAQHGLDVPYETGTGPNQQKRHQRQSLYQLMDKLSGFYQQALQAQTASDARHYLQQRGLSEAVINHFAIGF
APPGWDNVDKRFGRSAEDRASLNDAGMLVVNDNGRTYDRFRDRIMFPIRDKRGRVIAFGGRILGEGQPKYLNSPETDIFH
KGRQLYGLYEAQRQHPELSRLLVVEGYMDVVALAQFGIDYAVASLGTSTTAEHIQLLYRATDRVICCYDGDRAGREAAWR
ALENALPYLTDGRQLRFMFLPDGEDPDTLVRKIGKEAFEKYIEQAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLIG
QVPGETLRLYLRQQLGNKLGILDDSQLEKLLPKAALKANISPQSRIKRTTMRILIGLLVQHPRLSALVPTVQGLEQAIQP
GVPLFVELVQTCQAQPGLTTGQLLEHYRENKSYSQLETLATWNHMIIDEMIEATFVDTLASLYDSILEHRQEALIARDRT
HGLTAEERQELWSLNQALAKKT

Sequences:

>Translated_582_residues
MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHHEKTPSFTVNGEKQFYHCFGCGTHGNAIDFLMNYDRL
EFVESIEELAAQHGLDVPYETGTGPNQQKRHQRQSLYQLMDKLSGFYQQALQAQTASDARHYLQQRGLSEAVINHFAIGF
APPGWDNVDKRFGRSAEDRASLNDAGMLVVNDNGRTYDRFRDRIMFPIRDKRGRVIAFGGRILGEGQPKYLNSPETDIFH
KGRQLYGLYEAQRQHPELSRLLVVEGYMDVVALAQFGIDYAVASLGTSTTAEHIQLLYRATDRVICCYDGDRAGREAAWR
ALENALPYLTDGRQLRFMFLPDGEDPDTLVRKIGKEAFEKYIEQAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLIG
QVPGETLRLYLRQQLGNKLGILDDSQLEKLLPKAALKANISPQSRIKRTTMRILIGLLVQHPRLSALVPTVQGLEQAIQP
GVPLFVELVQTCQAQPGLTTGQLLEHYRENKSYSQLETLATWNHMIIDEMIEATFVDTLASLYDSILEHRQEALIARDRT
HGLTAEERQELWSLNQALAKKT
>Mature_581_residues
AGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHHEKTPSFTVNGEKQFYHCFGCGTHGNAIDFLMNYDRLE
FVESIEELAAQHGLDVPYETGTGPNQQKRHQRQSLYQLMDKLSGFYQQALQAQTASDARHYLQQRGLSEAVINHFAIGFA
PPGWDNVDKRFGRSAEDRASLNDAGMLVVNDNGRTYDRFRDRIMFPIRDKRGRVIAFGGRILGEGQPKYLNSPETDIFHK
GRQLYGLYEAQRQHPELSRLLVVEGYMDVVALAQFGIDYAVASLGTSTTAEHIQLLYRATDRVICCYDGDRAGREAAWRA
LENALPYLTDGRQLRFMFLPDGEDPDTLVRKIGKEAFEKYIEQAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLIGQ
VPGETLRLYLRQQLGNKLGILDDSQLEKLLPKAALKANISPQSRIKRTTMRILIGLLVQHPRLSALVPTVQGLEQAIQPG
VPLFVELVQTCQAQPGLTTGQLLEHYRENKSYSQLETLATWNHMIIDEMIEATFVDTLASLYDSILEHRQEALIARDRTH
GLTAEERQELWSLNQALAKKT

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=581, Percent_Identity=75.5593803786575, Blast_Score=926, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 65796; Mature: 65665

Theoretical pI: Translated: 6.99; Mature: 6.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHHEKTPSFTVNGEKQFYH
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEECCCCEEEE
CFGCGTHGNAIDFLMNYDRLEFVESIEELAAQHGLDVPYETGTGPNQQKRHQRQSLYQLM
EEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
DKLSGFYQQALQAQTASDARHYLQQRGLSEAVINHFAIGFAPPGWDNVDKRFGRSAEDRA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHC
SLNDAGMLVVNDNGRTYDRFRDRIMFPIRDKRGRVIAFGGRILGEGQPKYLNSPETDIFH
CCCCCCEEEECCCCCHHHHHHHHEECCEECCCCCEEEECCEEECCCCCCCCCCCCHHHHH
KGRQLYGLYEAQRQHPELSRLLVVEGYMDVVALAQFGIDYAVASLGTSTTAEHIQLLYRA
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TDRVICCYDGDRAGREAAWRALENALPYLTDGRQLRFMFLPDGEDPDTLVRKIGKEAFEK
CCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
YIEQAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLIGQVPGETLRLYLRQQLGNKLG
HHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
ILDDSQLEKLLPKAALKANISPQSRIKRTTMRILIGLLVQHPRLSALVPTVQGLEQAIQP
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
GVPLFVELVQTCQAQPGLTTGQLLEHYRENKSYSQLETLATWNHMIIDEMIEATFVDTLA
CCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLYDSILEHRQEALIARDRTHGLTAEERQELWSLNQALAKKT
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHHEKTPSFTVNGEKQFYH
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEECCCCEEEE
CFGCGTHGNAIDFLMNYDRLEFVESIEELAAQHGLDVPYETGTGPNQQKRHQRQSLYQLM
EEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHH
DKLSGFYQQALQAQTASDARHYLQQRGLSEAVINHFAIGFAPPGWDNVDKRFGRSAEDRA
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCHHHC
SLNDAGMLVVNDNGRTYDRFRDRIMFPIRDKRGRVIAFGGRILGEGQPKYLNSPETDIFH
CCCCCCEEEECCCCCHHHHHHHHEECCEECCCCCEEEECCEEECCCCCCCCCCCCHHHHH
KGRQLYGLYEAQRQHPELSRLLVVEGYMDVVALAQFGIDYAVASLGTSTTAEHIQLLYRA
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
TDRVICCYDGDRAGREAAWRALENALPYLTDGRQLRFMFLPDGEDPDTLVRKIGKEAFEK
CCCEEEEECCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHH
YIEQAQPLSTFLFETLMPQVDLSSPDGRAKLSTLALPLIGQVPGETLRLYLRQQLGNKLG
HHHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCC
ILDDSQLEKLLPKAALKANISPQSRIKRTTMRILIGLLVQHPRLSALVPTVQGLEQAIQP
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCC
GVPLFVELVQTCQAQPGLTTGQLLEHYRENKSYSQLETLATWNHMIIDEMIEATFVDTLA
CCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLYDSILEHRQEALIARDRTHGLTAEERQELWSLNQALAKKT
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]