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The map label for this gene is rpoD [H]

Identifier: 85058229

GI number: 85058229

Start: 465455

End: 467302

Strand: Reverse

Name: rpoD [H]

Synonym: SG0251

Alternate gene names: 85058229

Gene position: 467302-465455 (Counterclockwise)

Preceding gene: 85058230

Following gene: 85058228

Centisome position: 11.2

GC content: 55.74

Gene sequence:

>1848_bases
ATGGAGCAAAACCCGCAGTCACAGCTCAAGCTGCTTGTCACACGTGGTAAGGAGCAAGGCTATTTGACCTTTGCCGAGGT
CAATGACCATCTGCCGGAGGATATCGTCGACTCCGACCAGATCGAAGATATCATCCAGATGATAAATGACATGGGCATCC
AGGTGATGGAAGAAGCACCGGATGCCGATGACCTGCTGCTGGCCGAAACCACCTCCGACACCGACGAAGATGCGGCGGAA
GCCGCGGCGCAGGTCTTGTCCAGCGTGGAGTCCGAAATTGGGCGCACCACCGACCCCGTGCGCATGTACATGCGCGAAAT
GGGCACCGTGGAGCTGCTGACGCGCGAAGGCGAGATCGATATCGCCAAGCGTATCGAAGATGGCATCAATCAGGTTCAGT
GCTCGGTCGCCGAGTACCCTGAGGCTATCACCTACCTGCTGGAGCAGTACGACCGCGTCGAAGCCGGCGAGGCCCGCCTG
TCCGATCTTATCACCGGCTTCGTCGACCCTAACGCGGAAGAAGATATGGCGCCCACCGCGACCCATATCGGTTCCGAACT
CTCCGCCGACGAAATCGCCGATGACGACGACGAGGATGAAGAGGACGGCGACGACGACAGCACGGACGATGATAACAGCA
TCGATCCGGAGTTGGCGCGGCAAAAATTTGTCGAGCTGCGCGAGCAGTACGAAATTACCCGTAACGTTATAAAAACCAAC
GGGCGCAGCCATGCTAAATCAGCCTCGGAGATTTTGAATCTTTCCGATGTATTCAAACAGTTCCGCCTGGTGCCGAAGCA
GTTCGATTACCTGGTCAACAACATGCGCGCCATGATGGATCGCGTGCGTACCCAGGAACGTTTGATCATGAAATTGTGCG
TCGAAATCAGCAAGATGCCCAAGAAAAATTTCGTCACGCTGTTTGCCGGTAATGAAACTAGCGAAAGCTGGTTCAAAGCC
GCGCTGGCGATGAACAAGCCCTGGTCGGAAAAACTGCACGAAGTCGATGATGACGTGTACCGCAGCCTGCAGAAACTGCG
GCAGATTGAAGAAGAAACCGGTTTGACCATCGAGCAGGTGAAGGATATCAATCGCCGCATGTCGATCGGCGAAGCCAAGG
CGCGACGCGCCAAGAAAGAAATGGTCGAGGCGAACCTGCGTTTGGTTATCTCTATCGCCAAAAAATACACCAACCGCGGC
CTGCAGTTCCTGGATCTGATCCAGGAGGGCAATATCGGTTTGATGAAGGCGGTGGATAAATTTGAATACCGTCGTGGTTA
CAAGTTTTCCACCTACGCCACCTGGTGGATCCGCCAGGCCATCACTCGCTCTATCGCCGACCAGGCGCGCACCATCCGTA
TTCCGGTGCATATGATTGAGACCATCAACAAACTCAACCGCATTTCCCGACAGATGCTGCAGGAAATGGGACGCGAACCG
ACGCCCGAAGAGCTGGCGGAACGCATGCTGATGCCGGAGGATAAAATCCGTAAGGTGCTCAAGATTGCCAAAGAGCCTAT
CTCCATGGAAACTCCTATCGGCGACGATGAAGATTCGCACTTGGGGGATTTTATCGAGGACACCACCCTCGAACTGCCGC
TGGATTCCGCCACCTCGGAGAGTCTGCGTTCAGCCACCCACGATGTGCTGGCCGGCCTGACCGCGCGTGAAGCCAAGGTT
CTGCGCATGCGCTTCGGTATCGACATGAATACCGACCATACGCTGGAAGAAGTCGGCAAACAGTTTGACGTCACCCGCGA
GCGTATCCGTCAGATCGAAGCGAAAGCGCTGCGTAAGCTGCGGCACCCGAGCCGTTCTGAGGTCCTGCGCAGCTTCCTGG
ACGATTAA

Upstream 100 bases:

>100_bases
CGTATTGGGCTGCGGCGCGAAACAAAGCTCAGATCTCTTGTTGGTGATGCGCTTTCACCAACCAACACCGAATATTACTC
TGAAGTGTGGATACCGTCTT

Downstream 100 bases:

>100_bases
GTTCTGGTTTTGCTGGCGGTTCTTTCCTTAAGGCGCTCAAAACATCGCCCCGCAGAGCACACCGCGGGGTTTTTTATTGC
CCACCGCCCATGAGCGCTGG

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MEQNPQSQLKLLVTRGKEQGYLTFAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVMEEAPDADDLLLAETTSDTDEDAAE
AAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAGEARL
SDLITGFVDPNAEEDMAPTATHIGSELSADEIADDDDEDEEDGDDDSTDDDNSIDPELARQKFVELREQYEITRNVIKTN
GRSHAKSASEILNLSDVFKQFRLVPKQFDYLVNNMRAMMDRVRTQERLIMKLCVEISKMPKKNFVTLFAGNETSESWFKA
ALAMNKPWSEKLHEVDDDVYRSLQKLRQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRG
LQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREP
TPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATSESLRSATHDVLAGLTAREAKV
LRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD

Sequences:

>Translated_615_residues
MEQNPQSQLKLLVTRGKEQGYLTFAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVMEEAPDADDLLLAETTSDTDEDAAE
AAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAGEARL
SDLITGFVDPNAEEDMAPTATHIGSELSADEIADDDDEDEEDGDDDSTDDDNSIDPELARQKFVELREQYEITRNVIKTN
GRSHAKSASEILNLSDVFKQFRLVPKQFDYLVNNMRAMMDRVRTQERLIMKLCVEISKMPKKNFVTLFAGNETSESWFKA
ALAMNKPWSEKLHEVDDDVYRSLQKLRQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRG
LQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREP
TPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATSESLRSATHDVLAGLTAREAKV
LRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD
>Mature_615_residues
MEQNPQSQLKLLVTRGKEQGYLTFAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVMEEAPDADDLLLAETTSDTDEDAAE
AAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAGEARL
SDLITGFVDPNAEEDMAPTATHIGSELSADEIADDDDEDEEDGDDDSTDDDNSIDPELARQKFVELREQYEITRNVIKTN
GRSHAKSASEILNLSDVFKQFRLVPKQFDYLVNNMRAMMDRVRTQERLIMKLCVEISKMPKKNFVTLFAGNETSESWFKA
ALAMNKPWSEKLHEVDDDVYRSLQKLRQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRG
LQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREP
TPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATSESLRSATHDVLAGLTAREAKV
LRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=615, Percent_Identity=91.5447154471545, Blast_Score=1102, Evalue=0.0,
Organism=Escherichia coli, GI1789098, Length=247, Percent_Identity=42.1052631578947, Blast_Score=209, Evalue=4e-55,
Organism=Escherichia coli, GI1789871, Length=242, Percent_Identity=28.5123966942149, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=28.7128712871287, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007631
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04546 Sigma70_ner; PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 70380; Mature: 70380

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQNPQSQLKLLVTRGKEQGYLTFAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVMEEAP
CCCCHHHHHHHHHHCCCCCCCEEEEHHCCCCCHHHCCHHHHHHHHHHHHHHCHHHHHCCC
DADDLLLAETTSDTDEDAAEAAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEID
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCCCCHH
IAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAGEARLSDLITGFVDPNAEEDMAPTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
THIGSELSADEIADDDDEDEEDGDDDSTDDDNSIDPELARQKFVELREQYEITRNVIKTN
HHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GRSHAKSASEILNLSDVFKQFRLVPKQFDYLVNNMRAMMDRVRTQERLIMKLCVEISKMP
CCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKNFVTLFAGNETSESWFKAALAMNKPWSEKLHEVDDDVYRSLQKLRQIEEETGLTIEQV
CCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHH
KDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHH
FEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREP
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
TPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATSE
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEECCCCCCHHH
SLRSATHDVLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKL
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHPSRSEVLRSFLDD
CCCCHHHHHHHHHCC
>Mature Secondary Structure
MEQNPQSQLKLLVTRGKEQGYLTFAEVNDHLPEDIVDSDQIEDIIQMINDMGIQVMEEAP
CCCCHHHHHHHHHHCCCCCCCEEEEHHCCCCCHHHCCHHHHHHHHHHHHHHCHHHHHCCC
DADDLLLAETTSDTDEDAAEAAAQVLSSVESEIGRTTDPVRMYMREMGTVELLTREGEID
CCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHCCCCHH
IAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAGEARLSDLITGFVDPNAEEDMAPTA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHH
THIGSELSADEIADDDDEDEEDGDDDSTDDDNSIDPELARQKFVELREQYEITRNVIKTN
HHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
GRSHAKSASEILNLSDVFKQFRLVPKQFDYLVNNMRAMMDRVRTQERLIMKLCVEISKMP
CCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KKNFVTLFAGNETSESWFKAALAMNKPWSEKLHEVDDDVYRSLQKLRQIEEETGLTIEQV
CCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHH
KDINRRMSIGEAKARRAKKEMVEANLRLVISIAKKYTNRGLQFLDLIQEGNIGLMKAVDK
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHHHH
FEYRRGYKFSTYATWWIRQAITRSIADQARTIRIPVHMIETINKLNRISRQMLQEMGREP
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCC
TPEELAERMLMPEDKIRKVLKIAKEPISMETPIGDDEDSHLGDFIEDTTLELPLDSATSE
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHCCCEECCCCCCHHH
SLRSATHDVLAGLTAREAKVLRMRFGIDMNTDHTLEEVGKQFDVTRERIRQIEAKALRKL
HHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RHPSRSEVLRSFLDD
CCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]