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The map label for this gene is rpoN [H]

Identifier: 85058177

GI number: 85058177

Start: 378659

End: 380092

Strand: Reverse

Name: rpoN [H]

Synonym: SG0199

Alternate gene names: 85058177

Gene position: 380092-378659 (Counterclockwise)

Preceding gene: 85058178

Following gene: 85058176

Centisome position: 9.11

GC content: 56.69

Gene sequence:

>1434_bases
ATGAAGCAAGGTTTACAACTCAGGCTGAGTCAGCAGTTAGCCATGACCCCGCAGTTGCAACAGGCCATCCGTCTGTTGCA
ATTGTCCACGCTTGAACTCCAGCAGGAAATTCAGACGGCGCTGGAAAGTAACCCCTTTCTTGAACAGGCCGATTTGCATG
ACGAGGTCGACGCGCACAACGCGCCCGACAGCGAGTCGCTCGACACCCGCGAAGCGATGGAACAAAAGGACATGCCGGAA
GAGCTGCCGCTGGACGCCACCTGGGACGAGATGTACTCCGCCGGGACGCCCTCCGGCACCGGTACCGACTATCGCGACGA
AGAATTGCCCGTCTATCAGGGTGAAACCACCCAGACCCTGCAAAACTACCTCATGTGGCAGGTGGAGCTTACTCCCTTTT
CCGATACTGATAGAGCGATCGCCACCTCCATCGTCGACGCCGTGGACGACACCGGCTATCTGACGGTGCCGCTGGACGAT
ATTCTCGACAGCATCGGCGATGAAAACGTCACCCTGGACGAGGTGGAAGCGGTACTAAAACGCATCCAGCGCTTCGATCC
GGTCGGCGTGGCGGCACGGGATCTGCGCGACTGCCTGATGATTCAGCTCTCGCAGTTTGCGGCCGATCTGCCTTATCTCA
ATGAGGCGCGGCTCATCGTCAGAGATTATCTCAGTCTGCTGAGCAGCCATGATTTCCGCACCCTGCTACGTTCGACTAAG
CTGAAAGAGGATGTCCTGAAACCGGCCATAGCGCTGATTCAGTCGCTGGATCCGCGTCCGGGGCAGTCCATCAATACCGG
CGAGTCGCAGTATGTGATTCCCGACGTGCTGGTGCGCAAACTGCAAAACCACTGGGTGGTGGAACTCAATACCGACAGCA
TTCCCGCGCTGCGCATCAATCAGCAGTACGCGGCCCTGGGCAGCAACACCCGCAACGATGCGGACGGTCAGTTTATCCGC
AGCCATTTGCAGGAGGCGCGCTGGCTGATTAAAAGCCTGGAAAGCCGCAATGAAACGCTGCTGAAGGTGACTCACTGCAT
TGTCGAGCGGCAGATGGCGTTCTTTGAACAGGGGGAAGAGTTTATGCGGCCAATGGTGCTGGCGGATATCGCCCAGGCGG
TGGATATGCACGAGTCTACGATTTCGCGCATCACCATGCAGAAATATCTGCAAAGTCCACGCGGCATCTTTGAATTGAAA
TATTTTTTCTCCAGCTACGTCAATACCGAAGGGGGAGGCGAAGCGTCCTCCACGGCTATCCGCGCGCTGGTGAAAAAATT
AATCGCCGCGGAAAACCCGGCAAAGCCGCTTAGCGACAGTAAGCTCACCGACTTGCTTTCCGAGCAGGGTATCATGGTGG
CGCGGCGTACCGTAGCGAAGTACCGAGAGTCTTTATCCATACCGCCGTCGAACCAGCGAAAACAATTGGTTTGA

Upstream 100 bases:

>100_bases
TGCTCGACGAGCAGGTCAAACGGGTTTATCTGGGCGAAGAGTTCCGCCTCTGATAAGGTGGCGTTACCACCGTGGTTCGT
GAGGATAGTTGACGCATTTT

Downstream 100 bases:

>100_bases
CCTCTACAGAGAAGGAAGACACTATGCAGCTGAACATTACCGGACAACATGTTGACATCACCGACGCCTTACGGGAATTT
GTAACAACTAAATTCTCGAA

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQTALESNPFLEQADLHDEVDAHNAPDSESLDTREAMEQKDMPE
ELPLDATWDEMYSAGTPSGTGTDYRDEELPVYQGETTQTLQNYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLDD
ILDSIGDENVTLDEVEAVLKRIQRFDPVGVAARDLRDCLMIQLSQFAADLPYLNEARLIVRDYLSLLSSHDFRTLLRSTK
LKEDVLKPAIALIQSLDPRPGQSINTGESQYVIPDVLVRKLQNHWVVELNTDSIPALRINQQYAALGSNTRNDADGQFIR
SHLQEARWLIKSLESRNETLLKVTHCIVERQMAFFEQGEEFMRPMVLADIAQAVDMHESTISRITMQKYLQSPRGIFELK
YFFSSYVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTDLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Sequences:

>Translated_477_residues
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQTALESNPFLEQADLHDEVDAHNAPDSESLDTREAMEQKDMPE
ELPLDATWDEMYSAGTPSGTGTDYRDEELPVYQGETTQTLQNYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLDD
ILDSIGDENVTLDEVEAVLKRIQRFDPVGVAARDLRDCLMIQLSQFAADLPYLNEARLIVRDYLSLLSSHDFRTLLRSTK
LKEDVLKPAIALIQSLDPRPGQSINTGESQYVIPDVLVRKLQNHWVVELNTDSIPALRINQQYAALGSNTRNDADGQFIR
SHLQEARWLIKSLESRNETLLKVTHCIVERQMAFFEQGEEFMRPMVLADIAQAVDMHESTISRITMQKYLQSPRGIFELK
YFFSSYVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTDLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV
>Mature_477_residues
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQTALESNPFLEQADLHDEVDAHNAPDSESLDTREAMEQKDMPE
ELPLDATWDEMYSAGTPSGTGTDYRDEELPVYQGETTQTLQNYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLDD
ILDSIGDENVTLDEVEAVLKRIQRFDPVGVAARDLRDCLMIQLSQFAADLPYLNEARLIVRDYLSLLSSHDFRTLLRSTK
LKEDVLKPAIALIQSLDPRPGQSINTGESQYVIPDVLVRKLQNHWVVELNTDSIPALRINQQYAALGSNTRNDADGQFIR
SHLQEARWLIKSLESRNETLLKVTHCIVERQMAFFEQGEEFMRPMVLADIAQAVDMHESTISRITMQKYLQSPRGIFELK
YFFSSYVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTDLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=477, Percent_Identity=81.5513626834382, Blast_Score=809, Evalue=0.0,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53830; Mature: 53830

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQTALESNPFLEQADLHDEVDAHN
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHCCCC
APDSESLDTREAMEQKDMPEELPLDATWDEMYSAGTPSGTGTDYRDEELPVYQGETTQTL
CCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHH
QNYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLDDILDSIGDENVTLDEVEAVLK
HHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHH
RIQRFDPVGVAARDLRDCLMIQLSQFAADLPYLNEARLIVRDYLSLLSSHDFRTLLRSTK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
LKEDVLKPAIALIQSLDPRPGQSINTGESQYVIPDVLVRKLQNHWVVELNTDSIPALRIN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHCCCEEEEECCCCCCEEEEC
QQYAALGSNTRNDADGQFIRSHLQEARWLIKSLESRNETLLKVTHCIVERQMAFFEQGEE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHH
FMRPMVLADIAQAVDMHESTISRITMQKYLQSPRGIFELKYFFSSYVNTEGGGEASSTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
RALVKKLIAAENPAKPLSDSKLTDLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV
HHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQEIQTALESNPFLEQADLHDEVDAHN
CCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCHHHHCCCC
APDSESLDTREAMEQKDMPEELPLDATWDEMYSAGTPSGTGTDYRDEELPVYQGETTQTL
CCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHH
QNYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLDDILDSIGDENVTLDEVEAVLK
HHHHEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEEHHHHHHHHCCCCCCHHHHHHHHH
RIQRFDPVGVAARDLRDCLMIQLSQFAADLPYLNEARLIVRDYLSLLSSHDFRTLLRSTK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHH
LKEDVLKPAIALIQSLDPRPGQSINTGESQYVIPDVLVRKLQNHWVVELNTDSIPALRIN
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEECHHHHHHHHCCCEEEEECCCCCCEEEEC
QQYAALGSNTRNDADGQFIRSHLQEARWLIKSLESRNETLLKVTHCIVERQMAFFEQGEE
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHH
FMRPMVLADIAQAVDMHESTISRITMQKYLQSPRGIFELKYFFSSYVNTEGGGEASSTAI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHH
RALVKKLIAAENPAKPLSDSKLTDLLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV
HHHHHHHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2999700; 2695747 [H]