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The map label for this gene is fliF [H]

Identifier: 85058031

GI number: 85058031

Start: 69523

End: 71229

Strand: Reverse

Name: fliF [H]

Synonym: SG0053

Alternate gene names: 85058031

Gene position: 71229-69523 (Counterclockwise)

Preceding gene: 85058033

Following gene: 85058030

Centisome position: 1.71

GC content: 59.58

Gene sequence:

>1707_bases
ATGAATGCCTCGGCCATGGATATCAATGCTAAATCGGCAATAAGCCTGACCAACCTAATAGCGCGTTTTCGCGACCATCC
GAAATATATGCTATTAGCGGCAAGCGCCGCGGGAATCGCGCTGGCTATTGTCCTGCTGTTGTGGGCCAGAACGCCGGATT
ATCGCATTTTGTACAGCAATATCAGCGACCAGGACGGCGGAGCCGTGGTGGCGGAACTGACCACGATGAATATTCCCTAC
CGTTTTGCCGGCAACAGCGACGCCATTATGGTGCCGAGCGACCGGGTCCACGAAACCCGGTTGTTGCTGGCGCAAAAGGG
GTTGCCGAAGGGCGGCGCACTGGGATTTGAACTGCTGGATCGCGAGAAATTCGGCATGAGCCAATTCAGCGAGCAAATCA
ATTATCAGCGGGCGCTGGAAGGTGAAATCGCCCGCACCATTAAAACGCTAAATCCAGTGAAATCGGCCCGGGTTCATATC
GCGATGCCGAAACCCTCGCTGTTTGTGCGCGAACATAAGCCGGCGACCGCTTCGGTGACGTTGACGTTGTATCCCGGGCG
CGCGCTGGATACCGGTCAAATCCAGGCCATTACCTGGTTGGTATCGGGCACCGTGCCCGATTTACCCGCCGGAAATATTA
CCATGGTAGACAGCCAGGGCCAATTACTTGCCCAACCGGGCAATAATGAGCTTGACCTCAGCACGACCCAGCTTGCCTAT
ACCCGTGAAGTGGAGGGCGATTTTCAGAGGCGTATCAGCGCGATACTCGCGCCGGTGATAGGCGCAGCCAACGTGCGCGC
GGAGGTGACCGCCCAGTTGGACTTTACCGCGGTGGAGCAAACGGCGGAGCAGCATAAACCTAACGGGCGCCCGGAGGAAA
TGGCGATCCGCAGCCAACAATTGAGCGCCAGCGACCAGATCGGTATTCAAGCGCCTGGCGGTGTACCGGGGGCGCTTAGC
AATCAGCCCACACCGCCAGCCACGGCGTCGATTGAATCCCCCGCCAACGCCGCCGCCGGCAAAAAGCCCGCCTCCAAGGA
CAGTGTCCCGCCGCCCGCCGCCGCGGCACCCATCGTGCAGCCGCCGCGCAGCAGCCAGAACGAAAAAACCATTAATTACG
AAGTCGACCGTACCCTGACCCATAGCCGGTTTCGTCCCGGTGTCATTAAACGCTTGTCGGGGGCGGTGGTGGTTAACGAC
CGGGAGGATAGCAACGGCAATCGCGTGCATTTGACGCCGCAGGAAATGCAGGACGTGACGGCGTTGGTGCGCGAGGCCAT
GGGCTTTTCCAGCGAACGCGGCGATACGCTCAATATCGTTAATACCCGCTTCGTTGCCAATCAGGACATTATGCCGCCGC
TCCCGTTCTGGCAGGATCCGGGCCTGCAGTCTATCGCGTTCAGCCTCTTGCGCTATGCGCTGGTGGCGCTGCTGTTCTGG
CTGGCATGGCGCAAAGGGCTTCGTCCACTCTGGCAGCGGCAACAGGAGATCGAGCGCGAACGCATCCGGGCGCAGTACGA
AGCCAGCCAGCCGCGGGATGAACCGCGCCCCATTTCGCGCAGCGAGCTGGAGAAAGAAACACGTGAGAAAGCCCGCCAAG
CAATGGAGGACAGTATCCGACAGCTGCGCGATGTCGCCGCCGCCCGACCACAGATGGTGGCACGGATACTGCGTCAATGG
ATAACCCGTGAGCAACAACCATCATGA

Upstream 100 bases:

>100_bases
GTCATTGGGTGATGGAAAGCTATTCCCCAGTAATAAGCATTTTATTTCCTCACCCTACCACCATGATTCTTTGTTTTTAA
TTCTGCTTCAGGGATCCTGC

Downstream 100 bases:

>100_bases
ATGCCATCGAAAAAAGCGCCGTCGTCATGCTGACGCTGGGGGAAGAGGCCGCCGCCGCCGTCTTTAAACATCTGCGCAAG
CAGGAGGTGGAGGCAATAGG

Product: flagellar basal-body M-ring protein FliF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MNASAMDINAKSAISLTNLIARFRDHPKYMLLAASAAGIALAIVLLLWARTPDYRILYSNISDQDGGAVVAELTTMNIPY
RFAGNSDAIMVPSDRVHETRLLLAQKGLPKGGALGFELLDREKFGMSQFSEQINYQRALEGEIARTIKTLNPVKSARVHI
AMPKPSLFVREHKPATASVTLTLYPGRALDTGQIQAITWLVSGTVPDLPAGNITMVDSQGQLLAQPGNNELDLSTTQLAY
TREVEGDFQRRISAILAPVIGAANVRAEVTAQLDFTAVEQTAEQHKPNGRPEEMAIRSQQLSASDQIGIQAPGGVPGALS
NQPTPPATASIESPANAAAGKKPASKDSVPPPAAAAPIVQPPRSSQNEKTINYEVDRTLTHSRFRPGVIKRLSGAVVVND
REDSNGNRVHLTPQEMQDVTALVREAMGFSSERGDTLNIVNTRFVANQDIMPPLPFWQDPGLQSIAFSLLRYALVALLFW
LAWRKGLRPLWQRQQEIERERIRAQYEASQPRDEPRPISRSELEKETREKARQAMEDSIRQLRDVAAARPQMVARILRQW
ITREQQPS

Sequences:

>Translated_568_residues
MNASAMDINAKSAISLTNLIARFRDHPKYMLLAASAAGIALAIVLLLWARTPDYRILYSNISDQDGGAVVAELTTMNIPY
RFAGNSDAIMVPSDRVHETRLLLAQKGLPKGGALGFELLDREKFGMSQFSEQINYQRALEGEIARTIKTLNPVKSARVHI
AMPKPSLFVREHKPATASVTLTLYPGRALDTGQIQAITWLVSGTVPDLPAGNITMVDSQGQLLAQPGNNELDLSTTQLAY
TREVEGDFQRRISAILAPVIGAANVRAEVTAQLDFTAVEQTAEQHKPNGRPEEMAIRSQQLSASDQIGIQAPGGVPGALS
NQPTPPATASIESPANAAAGKKPASKDSVPPPAAAAPIVQPPRSSQNEKTINYEVDRTLTHSRFRPGVIKRLSGAVVVND
REDSNGNRVHLTPQEMQDVTALVREAMGFSSERGDTLNIVNTRFVANQDIMPPLPFWQDPGLQSIAFSLLRYALVALLFW
LAWRKGLRPLWQRQQEIERERIRAQYEASQPRDEPRPISRSELEKETREKARQAMEDSIRQLRDVAAARPQMVARILRQW
ITREQQPS
>Mature_568_residues
MNASAMDINAKSAISLTNLIARFRDHPKYMLLAASAAGIALAIVLLLWARTPDYRILYSNISDQDGGAVVAELTTMNIPY
RFAGNSDAIMVPSDRVHETRLLLAQKGLPKGGALGFELLDREKFGMSQFSEQINYQRALEGEIARTIKTLNPVKSARVHI
AMPKPSLFVREHKPATASVTLTLYPGRALDTGQIQAITWLVSGTVPDLPAGNITMVDSQGQLLAQPGNNELDLSTTQLAY
TREVEGDFQRRISAILAPVIGAANVRAEVTAQLDFTAVEQTAEQHKPNGRPEEMAIRSQQLSASDQIGIQAPGGVPGALS
NQPTPPATASIESPANAAAGKKPASKDSVPPPAAAAPIVQPPRSSQNEKTINYEVDRTLTHSRFRPGVIKRLSGAVVVND
REDSNGNRVHLTPQEMQDVTALVREAMGFSSERGDTLNIVNTRFVANQDIMPPLPFWQDPGLQSIAFSLLRYALVALLFW
LAWRKGLRPLWQRQQEIERERIRAQYEASQPRDEPRPISRSELEKETREKARQAMEDSIRQLRDVAAARPQMVARILRQW
ITREQQPS

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=553, Percent_Identity=49.7287522603978, Blast_Score=508, Evalue=1e-145,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 62565; Mature: 62565

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNASAMDINAKSAISLTNLIARFRDHPKYMLLAASAAGIALAIVLLLWARTPDYRILYSN
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEECC
ISDQDGGAVVAELTTMNIPYRFAGNSDAIMVPSDRVHETRLLLAQKGLPKGGALGFELLD
CCCCCCCEEEEEEEEECCCEEECCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHH
REKFGMSQFSEQINYQRALEGEIARTIKTLNPVKSARVHIAMPKPSLFVREHKPATASVT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCEEEEE
LTLYPGRALDTGQIQAITWLVSGTVPDLPAGNITMVDSQGQLLAQPGNNELDLSTTQLAY
EEEECCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEECCCCEEECCCCCCCCCCHHHEEE
TREVEGDFQRRISAILAPVIGAANVRAEVTAQLDFTAVEQTAEQHKPNGRPEEMAIRSQQ
EHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHH
LSASDQIGIQAPGGVPGALSNQPTPPATASIESPANAAAGKKPASKDSVPPPAAAAPIVQ
CCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PPRSSQNEKTINYEVDRTLTHSRFRPGVIKRLSGAVVVNDREDSNGNRVHLTPQEMQDVT
CCCCCCCCCEEEEEECCCHHHHCCCCHHHHHCCCCEEEECCCCCCCCEEEECHHHHHHHH
ALVREAMGFSSERGDTLNIVNTRFVANQDIMPPLPFWQDPGLQSIAFSLLRYALVALLFW
HHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LAWRKGLRPLWQRQQEIERERIRAQYEASQPRDEPRPISRSELEKETREKARQAMEDSIR
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QLRDVAAARPQMVARILRQWITREQQPS
HHHHHHHCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNASAMDINAKSAISLTNLIARFRDHPKYMLLAASAAGIALAIVLLLWARTPDYRILYSN
CCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHCCCCCCEEEECC
ISDQDGGAVVAELTTMNIPYRFAGNSDAIMVPSDRVHETRLLLAQKGLPKGGALGFELLD
CCCCCCCEEEEEEEEECCCEEECCCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCHHHHH
REKFGMSQFSEQINYQRALEGEIARTIKTLNPVKSARVHIAMPKPSLFVREHKPATASVT
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCEEEEE
LTLYPGRALDTGQIQAITWLVSGTVPDLPAGNITMVDSQGQLLAQPGNNELDLSTTQLAY
EEEECCCCCCCCCEEEEEEEEECCCCCCCCCCEEEEECCCCEEECCCCCCCCCCHHHEEE
TREVEGDFQRRISAILAPVIGAANVRAEVTAQLDFTAVEQTAEQHKPNGRPEEMAIRSQQ
EHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECHHHHHHHHHHCCCCCCHHHHHHHHHH
LSASDQIGIQAPGGVPGALSNQPTPPATASIESPANAAAGKKPASKDSVPPPAAAAPIVQ
CCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PPRSSQNEKTINYEVDRTLTHSRFRPGVIKRLSGAVVVNDREDSNGNRVHLTPQEMQDVT
CCCCCCCCCEEEEEECCCHHHHCCCCHHHHHCCCCEEEECCCCCCCCEEEECHHHHHHHH
ALVREAMGFSSERGDTLNIVNTRFVANQDIMPPLPFWQDPGLQSIAFSLLRYALVALLFW
HHHHHHHCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
LAWRKGLRPLWQRQQEIERERIRAQYEASQPRDEPRPISRSELEKETREKARQAMEDSIR
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
QLRDVAAARPQMVARILRQWITREQQPS
HHHHHHHCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2544561; 11677609; 1551848 [H]