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The map label for this gene is fliN [H]

Identifier: 85058023

GI number: 85058023

Start: 62870

End: 63274

Strand: Reverse

Name: fliN [H]

Synonym: SG0045

Alternate gene names: 85058023

Gene position: 63274-62870 (Counterclockwise)

Preceding gene: 85058024

Following gene: 85058022

Centisome position: 1.52

GC content: 60.49

Gene sequence:

>405_bases
ATGAGTGATACCCCACACACGCCCGGCGACGAGGCGCCCTTGCACGAAACCGACGGGCAAAGCCTTGGCGATACAGCATG
CCCGGCACCGAGCAGGCCGGAATTCCCCCCTGCTTCCACCACCGAAGGGGGATCGCCGCTGACGGAAGATCTCAGCCTGA
TTCTGGATATTCCGGTGAAAATGACCGTCGAATTGGGGCGGACCCGCATGACCATTCGCGAACTGCTGCACCTGAGCCAG
GACGCGGTAGTGCCTTTGGAAGGACAGGCGGGCGAACCGCTGGATATTCTGATAAACGGTTACCTGATCGCCCGCGGCGA
AGTCGTCGTAATGGGAGAACAATACGGCGTGCGCATCGCCGATATTGTTACGCCCGCCGAACGTATGCGCCGGTTGAGCC
GATAA

Upstream 100 bases:

>100_bases
TTCCTCGGCAACTACGGCAGCCTGAACGGGCGGTATGCATTGCGAGTAGAACACGTGATTAATCAAGGCATTGACCAATT
AAACAATAAGGACATTCCTC

Downstream 100 bases:

>100_bases
ATGAAACAGACCACACTACAGCCGCCGGTCGCGCATGACAGCGGCCACGGCACCGTACTTTTCAACGTGGCCGGATCGCT
GGGGGCGGTATTGCTGCTGA

Product: flagellar motor switch protein FliN

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 133

Protein sequence:

>134_residues
MSDTPHTPGDEAPLHETDGQSLGDTACPAPSRPEFPPASTTEGGSPLTEDLSLILDIPVKMTVELGRTRMTIRELLHLSQ
DAVVPLEGQAGEPLDILINGYLIARGEVVVMGEQYGVRIADIVTPAERMRRLSR

Sequences:

>Translated_134_residues
MSDTPHTPGDEAPLHETDGQSLGDTACPAPSRPEFPPASTTEGGSPLTEDLSLILDIPVKMTVELGRTRMTIRELLHLSQ
DAVVPLEGQAGEPLDILINGYLIARGEVVVMGEQYGVRIADIVTPAERMRRLSR
>Mature_133_residues
SDTPHTPGDEAPLHETDGQSLGDTACPAPSRPEFPPASTTEGGSPLTEDLSLILDIPVKMTVELGRTRMTIRELLHLSQD
AVVPLEGQAGEPLDILINGYLIARGEVVVMGEQYGVRIADIVTPAERMRRLSR

Specific function: FliN is one of three proteins (FliG, FliN, FliM) that form a switch complex that is proposed to be located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the motor

COG id: COG1886

COG function: function code NU; Flagellar motor switch/type III secretory pathway protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliN/MopA/SpaO family [H]

Homologues:

Organism=Escherichia coli, GI1788256, Length=86, Percent_Identity=75.5813953488372, Blast_Score=138, Evalue=1e-34,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012826
- InterPro:   IPR001172
- InterPro:   IPR001543 [H]

Pfam domain/function: PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 14455; Mature: 14323

Theoretical pI: Translated: 4.33; Mature: 4.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTPHTPGDEAPLHETDGQSLGDTACPAPSRPEFPPASTTEGGSPLTEDLSLILDIPVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEECCCCE
MTVELGRTRMTIRELLHLSQDAVVPLEGQAGEPLDILINGYLIARGEVVVMGEQYGVRIA
EEEECCHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEECEEEECCCEEEECCCCCEEEH
DIVTPAERMRRLSR
HHCCCHHHHHHHCC
>Mature Secondary Structure 
SDTPHTPGDEAPLHETDGQSLGDTACPAPSRPEFPPASTTEGGSPLTEDLSLILDIPVK
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHEEECCCCE
MTVELGRTRMTIRELLHLSQDAVVPLEGQAGEPLDILINGYLIARGEVVVMGEQYGVRIA
EEEECCHHHHHHHHHHHCCCCCEEECCCCCCCCEEEEEECEEEECCCEEEECCCCCEEEH
DIVTPAERMRRLSR
HHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2651416; 9097040; 9278503 [H]