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The map label for this gene is trmE [H]

Identifier: 84626025

GI number: 84626025

Start: 4933351

End: 4934691

Strand: Reverse

Name: trmE [H]

Synonym: XOO_4368

Alternate gene names: 84626025

Gene position: 4934691-4933351 (Counterclockwise)

Preceding gene: 84626026

Following gene: 84626023

Centisome position: 99.89

GC content: 65.32

Gene sequence:

>1341_bases
ATGTCGTCTTCCACCTCCACCATCGTTGCCATCGCCAGTGCTGCCGGAACCGGTGGCGTGGGCATCGTGCGCCTGTCCGG
GCCGCAGTCGCGGCAGATTGCAGTGCAATTAGGTGTTGCGCGTCTGCAGCCGCGGCATGCGCACTACGCGCGTTTCCGCG
ATGCGCAGGGCGCGGTGATCGATGATGGCATTGCGCTGTGGTTCAACGCGCCGCACAGTTTTACCGGCGAAGATGTTGTC
GAGTTGCAAGGCCATGGCAGCCCGGTACTGTTGCGGCAACTGGTTGCCCGTTGCATCGAATTGGGTGCACGTCAGGCACG
CGCCGGTGAGTTCAGTGAGCGTGCGTTCCTCAACGGCAAACTCGATCTTGCGCAGGCCGAAGCGATCGCCGACGTGATCG
CAGCCGGCGACCTGCGGGCGGCACGCGCCGCGCGGCGCGCACTGGATGGCGTGTTTTCGCGACGTGTCGATGCAGTGGCC
CACACCCTGACCCGTCTGCGCATCCATGTCGAAGCGGCCATCGACTTTGCCGACGAGCCGCTCGACACCTTGGGCGGCAA
CCAGGTACGCGACGGCTTGACCCAGGCGCGCACCCTGCTCGCCCAGTTGCTGCGCGATGCCGAACGCGGACGCACGTTGC
GCGACGGCCTGCATGCGGTGTTGATCGGCCCGCCCAACGCCGGCAAAAGTTCGTTGCTCAATGCACTGGCCGGTAGCGAG
CGCGCGATCGTCACCGATGTCGCCGGCACCACCCGCGACACCTTGCACGAAGCCATCCAGCTCGACGGCTTCGAGTTGAC
CCTGGTCGATACCGCGGGCCTGCGCGATGGTGGCGATGCGATCGAACGCGAAGGCATGCGTCGCGCCCGCGCCGAACTGG
AGCGCGCCGATCTGGCCTTGGTGGTGCTGGATGCACGCGATCCGCAGGCGGCACGCGCGGCGATCGGCGATGCCATCGAT
GCAGTACCGCGTCAGCTGTGGATCCATAACAAATGCGATCTGCTCAGCGATGCCGCGCCACTCGACGTCAATGCGATTGC
CGTCTCGGCGGTGACCGGTCAAGGACTTGAGCAACTGCACATCCGCCTGCGCGAGCTTGCGCTCGGCGATGGCGTGGAAG
GCGTCGATGGCGAGTTCTCTGCCCGCACGCGGCACGTGGAGGCGCTGCGTCGCGCCGAACGACACGTGGACGCCGCCGAT
TTGGAACTGGGCTTCGAACAACTCGAACTGGCCGCCGAAGAACTGCGCCTGGCGCATGAAGCGCTCGGCGAGATCACCGG
GAAGATCAGCGCCGATGACTTGCTCGGCAAGATCTTTTCGAGTTTCTGTATCGGCAAGTGA

Upstream 100 bases:

>100_bases
GGATGAGCCGGAAGGACCCGCGCACAGGTAATAGGCCGCTGGCGATCGTGCAGTGGATTCCATAGACATTTCCCTTTCAA
CCCACGTCAGAGCAGTGCCC

Downstream 100 bases:

>100_bases
TGCGGTCGTGCTCAGCGTATTGCTACACGCTGAGGTTGTCGGAGTCAGCCCGTGCCACAAGTGCGTATTCGCTTAGCACA
CCACCTGCGTAGTTAGCGCT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 445

Protein sequence:

>446_residues
MSSSTSTIVAIASAAGTGGVGIVRLSGPQSRQIAVQLGVARLQPRHAHYARFRDAQGAVIDDGIALWFNAPHSFTGEDVV
ELQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGKLDLAQAEAIADVIAAGDLRAARAARRALDGVFSRRVDAVA
HTLTRLRIHVEAAIDFADEPLDTLGGNQVRDGLTQARTLLAQLLRDAERGRTLRDGLHAVLIGPPNAGKSSLLNALAGSE
RAIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLALVVLDARDPQAARAAIGDAID
AVPRQLWIHNKCDLLSDAAPLDVNAIAVSAVTGQGLEQLHIRLRELALGDGVEGVDGEFSARTRHVEALRRAERHVDAAD
LELGFEQLELAAEELRLAHEALGEITGKISADDLLGKIFSSFCIGK

Sequences:

>Translated_446_residues
MSSSTSTIVAIASAAGTGGVGIVRLSGPQSRQIAVQLGVARLQPRHAHYARFRDAQGAVIDDGIALWFNAPHSFTGEDVV
ELQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGKLDLAQAEAIADVIAAGDLRAARAARRALDGVFSRRVDAVA
HTLTRLRIHVEAAIDFADEPLDTLGGNQVRDGLTQARTLLAQLLRDAERGRTLRDGLHAVLIGPPNAGKSSLLNALAGSE
RAIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLALVVLDARDPQAARAAIGDAID
AVPRQLWIHNKCDLLSDAAPLDVNAIAVSAVTGQGLEQLHIRLRELALGDGVEGVDGEFSARTRHVEALRRAERHVDAAD
LELGFEQLELAAEELRLAHEALGEITGKISADDLLGKIFSSFCIGK
>Mature_445_residues
SSSTSTIVAIASAAGTGGVGIVRLSGPQSRQIAVQLGVARLQPRHAHYARFRDAQGAVIDDGIALWFNAPHSFTGEDVVE
LQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGKLDLAQAEAIADVIAAGDLRAARAARRALDGVFSRRVDAVAH
TLTRLRIHVEAAIDFADEPLDTLGGNQVRDGLTQARTLLAQLLRDAERGRTLRDGLHAVLIGPPNAGKSSLLNALAGSER
AIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLALVVLDARDPQAARAAIGDAIDA
VPRQLWIHNKCDLLSDAAPLDVNAIAVSAVTGQGLEQLHIRLRELALGDGVEGVDGEFSARTRHVEALRRAERHVDAADL
ELGFEQLELAAEELRLAHEALGEITGKISADDLLGKIFSSFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=466, Percent_Identity=40.1287553648069, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI193082988, Length=498, Percent_Identity=38.1526104417671, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI193082993, Length=455, Percent_Identity=38.6813186813187, Blast_Score=238, Evalue=1e-62,
Organism=Escherichia coli, GI2367268, Length=455, Percent_Identity=49.4505494505495, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI87082120, Length=90, Percent_Identity=41.1111111111111, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=462, Percent_Identity=30.952380952381, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6323665, Length=500, Percent_Identity=29.2, Blast_Score=187, Evalue=2e-48,
Organism=Drosophila melanogaster, GI45550813, Length=481, Percent_Identity=33.6798336798337, Blast_Score=210, Evalue=2e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 47481; Mature: 47350

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSTSTIVAIASAAGTGGVGIVRLSGPQSRQIAVQLGVARLQPRHAHYARFRDAQGAVI
CCCCCCEEEEEEECCCCCCEEEEEECCCCCCEEEEEHHHHHCCCCHHHHHHHCCCCCCEE
DDGIALWFNAPHSFTGEDVVELQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGK
ECCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHCCCCCHHHHHCCCC
LDLAQAEAIADVIAAGDLRAARAARRALDGVFSRRVDAVAHTLTRLRIHVEAAIDFADEP
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCCCC
LDTLGGNQVRDGLTQARTLLAQLLRDAERGRTLRDGLHAVLIGPPNAGKSSLLNALAGSE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCHHHHHHHHCCCC
RAIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLAL
CEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEE
VVLDARDPQAARAAIGDAIDAVPRQLWIHNKCDLLSDAAPLDVNAIAVSAVTGQGLEQLH
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHEEEEEHHCCCHHHHH
IRLRELALGDGVEGVDGEFSARTRHVEALRRAERHVDAADLELGFEQLELAAEELRLAHE
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHH
ALGEITGKISADDLLGKIFSSFCIGK
HHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSSTSTIVAIASAAGTGGVGIVRLSGPQSRQIAVQLGVARLQPRHAHYARFRDAQGAVI
CCCCCEEEEEEECCCCCCEEEEEECCCCCCEEEEEHHHHHCCCCHHHHHHHCCCCCCEE
DDGIALWFNAPHSFTGEDVVELQGHGSPVLLRQLVARCIELGARQARAGEFSERAFLNGK
ECCEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCHHHCCCCCHHHHHCCCC
LDLAQAEAIADVIAAGDLRAARAARRALDGVFSRRVDAVAHTLTRLRIHVEAAIDFADEP
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCCCC
LDTLGGNQVRDGLTQARTLLAQLLRDAERGRTLRDGLHAVLIGPPNAGKSSLLNALAGSE
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCHHHHHHHHCCCC
RAIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLRDGGDAIEREGMRRARAELERADLAL
CEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCEEE
VVLDARDPQAARAAIGDAIDAVPRQLWIHNKCDLLSDAAPLDVNAIAVSAVTGQGLEQLH
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCHHEEEEEHHCCCHHHHH
IRLRELALGDGVEGVDGEFSARTRHVEALRRAERHVDAADLELGFEQLELAAEELRLAHE
HHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCHHHHHHHHHHHHHHHH
ALGEITGKISADDLLGKIFSSFCIGK
HHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]