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The map label for this gene is rlmI [H]

Identifier: 84626018

GI number: 84626018

Start: 4924835

End: 4926001

Strand: Reverse

Name: rlmI [H]

Synonym: XOO_4361

Alternate gene names: 84626018

Gene position: 4926001-4924835 (Counterclockwise)

Preceding gene: 84626021

Following gene: 84626017

Centisome position: 99.71

GC content: 63.92

Gene sequence:

>1167_bases
ATGAATAGTCCAGTCCCCGTCGTCCGTCTCAAGAATGCCTGGCGCTCCAGCCACCCGTGGATCTTCCAGAAGTTGGTGGA
AAAGCCCGCCGCCAAGCCCAAGCCCGGCACCATCGTCGATGTGGTCGGCGTGGACGGGGAGTGGATCGGCCGCGGCTTCT
ACAACGGCCATTCGCGCATTGCCGTGCGCATTCTGGAAACCGACCAGTCGGTGCCGGTGGATGCGGGCTGGTTTGCGCGC
AAGATCGCCGCGGCGGTGAGCCTGCGCCGCGATTGGCTCAAGCTCGATGGCGTGTCCGATGCCTGGCGCGTGGTGCACAG
CGAAGGCGACGGCCTGTCCGGCCTGGTGGTGGACCGCTATGGCGACCTGGTGGTGGTGGAGTTCTTCGCCGCCGGCATGT
TCCGTCACCGCGAATGGATCTACGACGCGCTGCGCGAACAGTTCCCGGGCTGCCGCTTCCACAGCTTTGCCGACGAACAC
GTGCAGAAGCAGGAAAGCTTCGACTTCCACAGCAACACCACCACCGAAGCGGCGGTGATCACCGAGCACGGCATCAAGTT
CCGCGCCGACCCGGCCGGTGCGCACAAGACCGGCTTCTTCGCCGATCAGCGCGACAACCGTCAGTGGCTGAGCGAGCAGG
TCGAAGGCAAGACCGTGCTGGACCTGTGCTGCAACACCGGCGGCTTTGCGGTGTATGCCGCCGCGCGCGGTGCGTCCGAA
GTGCTGGGCGTGGATATCGACGAAGACGTCATCGCCATTGCCAAGGGCAACGCCAAGCTCAACAACGTGCGCCCCAAGTT
CGTACAGGCCGACATCTTTCCGTGGCTGCGCGATGCGTCCAATCGGGGTGAGCAGTTCGATGTGGTGATCCTGGACCCGG
CCAAGATGACCCGCGACCGCGCGCAGGTAATTCCGGCGCTGAAGAAGTACCTGGACATGAACAAATTGGCGCTGGGCGTG
GTCAAGCCCGGTGGTCTGTTCGCCACGTTCTCGTGCACCGGCCTGGTGGCCGAGCAGGAGTTTCTGGACATGCTACGGCG
TGCCTCGTATTTCTCCGGCCGCACCATCCAGATCCTGAAAGTGGCCGGCGCCGGCCCGGATCACCCATTCATGGCGCACG
TGCAGGAATCGCGCTATTTGAAGGCCGTTTTCTGCCGCGTGGTGTAA

Upstream 100 bases:

>100_bases
GGCTGGTCATGACGGCGACAACGCTGGGAGCTACTCCCTTTCTGGCCCGATTCTGCGTGCCCGCCGCAGGCACGTCAATC
CAGCCGGATACAATGGGCCG

Downstream 100 bases:

>100_bases
CCGGGTTGCACGGCCGAAGACGCGGTCTGCCGCATTGCAGGCAGCGTCTTCCCTGGCGCGATGCCATCGTCGGCAAGCGT
CGATTCAACGCAGAGCTACA

Product: oxidoreductase

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MNSPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGTIVDVVGVDGEWIGRGFYNGHSRIAVRILETDQSVPVDAGWFAR
KIAAAVSLRRDWLKLDGVSDAWRVVHSEGDGLSGLVVDRYGDLVVVEFFAAGMFRHREWIYDALREQFPGCRFHSFADEH
VQKQESFDFHSNTTTEAAVITEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKTVLDLCCNTGGFAVYAAARGASE
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDASNRGEQFDVVILDPAKMTRDRAQVIPALKKYLDMNKLALGV
VKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILKVAGAGPDHPFMAHVQESRYLKAVFCRVV

Sequences:

>Translated_388_residues
MNSPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGTIVDVVGVDGEWIGRGFYNGHSRIAVRILETDQSVPVDAGWFAR
KIAAAVSLRRDWLKLDGVSDAWRVVHSEGDGLSGLVVDRYGDLVVVEFFAAGMFRHREWIYDALREQFPGCRFHSFADEH
VQKQESFDFHSNTTTEAAVITEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKTVLDLCCNTGGFAVYAAARGASE
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDASNRGEQFDVVILDPAKMTRDRAQVIPALKKYLDMNKLALGV
VKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILKVAGAGPDHPFMAHVQESRYLKAVFCRVV
>Mature_388_residues
MNSPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGTIVDVVGVDGEWIGRGFYNGHSRIAVRILETDQSVPVDAGWFAR
KIAAAVSLRRDWLKLDGVSDAWRVVHSEGDGLSGLVVDRYGDLVVVEFFAAGMFRHREWIYDALREQFPGCRFHSFADEH
VQKQESFDFHSNTTTEAAVITEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKTVLDLCCNTGGFAVYAAARGASE
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDASNRGEQFDVVILDPAKMTRDRAQVIPALKKYLDMNKLALGV
VKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILKVAGAGPDHPFMAHVQESRYLKAVFCRVV

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=379, Percent_Identity=35.0923482849604, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1787181, Length=197, Percent_Identity=26.3959390862944, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 43219; Mature: 43219

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGTIVDVVGVDGEWIGRGFYNGHSRI
CCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCEECCCEECCCCEE
AVRILETDQSVPVDAGWFARKIAAAVSLRRDWLKLDGVSDAWRVVHSEGDGLSGLVVDRY
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCHHHHHEECCCCCCCEEEEECC
GDLVVVEFFAAGMFRHREWIYDALREQFPGCRFHSFADEHVQKQESFDFHSNTTTEAAVI
CCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEE
TEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKTVLDLCCNTGGFAVYAAARGASE
EECCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEHHHHCCCCEEEEEECCCCCH
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDASNRGEQFDVVILDPAKMTRDR
HEECCCCCCEEEEEECCCEECCCCCCCEEHHHHHHHHHHCCCCCEEEEEEECCHHHHHHH
AQVIPALKKYLDMNKLALGVVKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILK
HHHHHHHHHHHCCCHHEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEE
VAGAGPDHPFMAHVQESRYLKAVFCRVV
EECCCCCCCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSPVPVVRLKNAWRSSHPWIFQKLVEKPAAKPKPGTIVDVVGVDGEWIGRGFYNGHSRI
CCCCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEEECCCCEECCCEECCCCEE
AVRILETDQSVPVDAGWFARKIAAAVSLRRDWLKLDGVSDAWRVVHSEGDGLSGLVVDRY
EEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHEECCCCHHHHHEECCCCCCCEEEEECC
GDLVVVEFFAAGMFRHREWIYDALREQFPGCRFHSFADEHVQKQESFDFHSNTTTEAAVI
CCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEE
TEHGIKFRADPAGAHKTGFFADQRDNRQWLSEQVEGKTVLDLCCNTGGFAVYAAARGASE
EECCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCEEEHHHHCCCCEEEEEECCCCCH
VLGVDIDEDVIAIAKGNAKLNNVRPKFVQADIFPWLRDASNRGEQFDVVILDPAKMTRDR
HEECCCCCCEEEEEECCCEECCCCCCCEEHHHHHHHHHHCCCCCEEEEEEECCHHHHHHH
AQVIPALKKYLDMNKLALGVVKPGGLFATFSCTGLVAEQEFLDMLRRASYFSGRTIQILK
HHHHHHHHHHHCCCHHEEEEECCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEE
VAGAGPDHPFMAHVQESRYLKAVFCRVV
EECCCCCCCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA