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The map label for this gene is kgtP [H]

Identifier: 84625997

GI number: 84625997

Start: 4904813

End: 4906174

Strand: Reverse

Name: kgtP [H]

Synonym: XOO_4340

Alternate gene names: 84625997

Gene position: 4906174-4904813 (Counterclockwise)

Preceding gene: 84625998

Following gene: 84625993

Centisome position: 99.31

GC content: 61.75

Gene sequence:

>1362_bases
ATGAGCGCACCCCCATCGTCTCCGATCGCCATCGGTTCTGGCACCGCTGCCGGTAGCCTTCGCCGCTCGGTGTCCAACAC
GCTCAAGGGCTCGGCCGGCAACCTGGTCGAGTGGTACGACGTTTACGTTTATTCGGTGTTCGCCACGTACTTCGAATCGC
AGTTCTTTTCCAAGCAAGACAAGAACGCCACCATGTTCGTGTGGGCGATCTTTGCGATGACGTTTTTGATGCGGCCGATC
GGTGCGTGGTATTTCGGTCGTTTCGCCGACCGCTACGGCCGCCGCCTGGCGTTGACCATTTCGGTGTCGATGATGGCGCT
GTGCTCGTTCGTCATCGCGATCACGCCGACCGTGGCGACAATCGGCATCGCGGCGCCGATCATCCTGTTGATGGCGCGCT
TGCTGCAAGGCTTTGCCACCGGTGGCGAATACGGCACCAGCGCCACGTACATGTCTGAAGCGGCGATTCCGGGCCGGCGC
GGGTTTTTGTCGTCGTTCCATTACGTGACATTGGTAGGCGGCCATGTGCTGGCGCAGACCACCTTGTTGATCATGCTGCA
TTTCTTCGATGCGCCGCAGGTCTCCGAGTGGGGCTGGCGCGTGGCCTTCGGGCTGGGCGGTATCGGCGCGCTGGTAGTGT
TCTGGCTGCGCCGCACGATGGACGAGTCGCTGAGCTCCGAATCCATCGCCGATTCGCGTACCGGCAAGGCCAAGGCGTCC
GGCTCGATGCGCGAATTGTTCGTCAATCAGTGGCGTCCGCTGCTGTTGTGCTTCCTGATCACTGCGGGCGGCACGATCGC
GTTCTACACCTATTCGGTGACCGGGCCGAAGATGATCCAGGCCGCGTTCGCCGGTGGCGACGTGATGGCCGGCACCGTCA
TCAACCTGGTGGCGCTGACCGTGCTGATGCTGATGCAGCCGGTAGGCGGCTGGTTGTCGGACATCCTCGGCCGCAAGACC
TTGCTGGTGTTCTTCGGCATCGGCGGTGTGCTCTACACCTGGTTCTTGGTGATGGAGCTGCCCAAGCAGACCGACTGGCT
GACCGCGTTCGCGATCCTCACCGTGGGCTTTGTGATCCTGACCGGCTACACCTCGATCAATGCGCTGGTGAAGGCGGAAT
TGTTCCCTGCCCACGTGCGCGCGCTAGGCGTTGGTCTTGGCTACGCGCTGGCAAATTCCGCGTTCGGCGGAACCGCGCCG
TTGCTGTATCAGGCCTCGCTGAAGACCAACCACGTGACCGAGTTCGTGATCTATGCGACCGCGGTGATTACGGTGTCGTT
GGTGGTCTACATGGTCTTCCTGACCAACAAGGGCAGCAATTGGCTGGACGACGAAGCGGCGATGCATCAGCGCAAGCGCT
GA

Upstream 100 bases:

>100_bases
GTGCTCGATTTGCGGCTGTATATGCGCCGAAATGCTTAGATGCGTCACACAAGCGCGCACCTATACTTCGGGGCTGTCCC
ACCGAAGAGGTTCTGACCGC

Downstream 100 bases:

>100_bases
TGAGTTGCACGCGGTACTCCTTCTCCCTTCGGGACCTATTGTCCCCCTTCATGGGGGAGCAGGGGCCCCGTAGGGGCGGA
TGAGGGTGCGCCTCAGGTAA

Product: dicarboxylate transport protein

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSAPPSSPIAIGSGTAAGSLRRSVSNTLKGSAGNLVEWYDVYVYSVFATYFESQFFSKQDKNATMFVWAIFAMTFLMRPI
GAWYFGRFADRYGRRLALTISVSMMALCSFVIAITPTVATIGIAAPIILLMARLLQGFATGGEYGTSATYMSEAAIPGRR
GFLSSFHYVTLVGGHVLAQTTLLIMLHFFDAPQVSEWGWRVAFGLGGIGALVVFWLRRTMDESLSSESIADSRTGKAKAS
GSMRELFVNQWRPLLLCFLITAGGTIAFYTYSVTGPKMIQAAFAGGDVMAGTVINLVALTVLMLMQPVGGWLSDILGRKT
LLVFFGIGGVLYTWFLVMELPKQTDWLTAFAILTVGFVILTGYTSINALVKAELFPAHVRALGVGLGYALANSAFGGTAP
LLYQASLKTNHVTEFVIYATAVITVSLVVYMVFLTNKGSNWLDDEAAMHQRKR

Sequences:

>Translated_453_residues
MSAPPSSPIAIGSGTAAGSLRRSVSNTLKGSAGNLVEWYDVYVYSVFATYFESQFFSKQDKNATMFVWAIFAMTFLMRPI
GAWYFGRFADRYGRRLALTISVSMMALCSFVIAITPTVATIGIAAPIILLMARLLQGFATGGEYGTSATYMSEAAIPGRR
GFLSSFHYVTLVGGHVLAQTTLLIMLHFFDAPQVSEWGWRVAFGLGGIGALVVFWLRRTMDESLSSESIADSRTGKAKAS
GSMRELFVNQWRPLLLCFLITAGGTIAFYTYSVTGPKMIQAAFAGGDVMAGTVINLVALTVLMLMQPVGGWLSDILGRKT
LLVFFGIGGVLYTWFLVMELPKQTDWLTAFAILTVGFVILTGYTSINALVKAELFPAHVRALGVGLGYALANSAFGGTAP
LLYQASLKTNHVTEFVIYATAVITVSLVVYMVFLTNKGSNWLDDEAAMHQRKR
>Mature_452_residues
SAPPSSPIAIGSGTAAGSLRRSVSNTLKGSAGNLVEWYDVYVYSVFATYFESQFFSKQDKNATMFVWAIFAMTFLMRPIG
AWYFGRFADRYGRRLALTISVSMMALCSFVIAITPTVATIGIAAPIILLMARLLQGFATGGEYGTSATYMSEAAIPGRRG
FLSSFHYVTLVGGHVLAQTTLLIMLHFFDAPQVSEWGWRVAFGLGGIGALVVFWLRRTMDESLSSESIADSRTGKAKASG
SMRELFVNQWRPLLLCFLITAGGTIAFYTYSVTGPKMIQAAFAGGDVMAGTVINLVALTVLMLMQPVGGWLSDILGRKTL
LVFFGIGGVLYTWFLVMELPKQTDWLTAFAILTVGFVILTGYTSINALVKAELFPAHVRALGVGLGYALANSAFGGTAPL
LYQASLKTNHVTEFVIYATAVITVSLVVYMVFLTNKGSNWLDDEAAMHQRKR

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=436, Percent_Identity=41.0550458715596, Blast_Score=287, Evalue=8e-79,
Organism=Escherichia coli, GI1790550, Length=375, Percent_Identity=27.7333333333333, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1788292, Length=396, Percent_Identity=29.7979797979798, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789941, Length=387, Percent_Identity=30.4909560723514, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI87082231, Length=221, Percent_Identity=27.1493212669683, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082404, Length=354, Percent_Identity=25.4237288135593, Blast_Score=68, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49163; Mature: 49032

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPPSSPIAIGSGTAAGSLRRSVSNTLKGSAGNLVEWYDVYVYSVFATYFESQFFSKQD
CCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KNATMFVWAIFAMTFLMRPIGAWYFGRFADRYGRRLALTISVSMMALCSFVIAITPTVAT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHH
IGIAAPIILLMARLLQGFATGGEYGTSATYMSEAAIPGRRGFLSSFHYVTLVGGHVLAQT
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHCCHHHHHCCHHHHHH
TLLIMLHFFDAPQVSEWGWRVAFGLGGIGALVVFWLRRTMDESLSSESIADSRTGKAKAS
HHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCC
GSMRELFVNQWRPLLLCFLITAGGTIAFYTYSVTGPKMIQAAFAGGDVMAGTVINLVALT
CHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHH
VLMLMQPVGGWLSDILGRKTLLVFFGIGGVLYTWFLVMELPKQTDWLTAFAILTVGFVIL
HHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
TGYTSINALVKAELFPAHVRALGVGLGYALANSAFGGTAPLLYQASLKTNHVTEFVIYAT
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHH
AVITVSLVVYMVFLTNKGSNWLDDEAAMHQRKR
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SAPPSSPIAIGSGTAAGSLRRSVSNTLKGSAGNLVEWYDVYVYSVFATYFESQFFSKQD
CCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KNATMFVWAIFAMTFLMRPIGAWYFGRFADRYGRRLALTISVSMMALCSFVIAITPTVAT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHH
IGIAAPIILLMARLLQGFATGGEYGTSATYMSEAAIPGRRGFLSSFHYVTLVGGHVLAQT
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCCHHHHHHCCHHHHHCCHHHHHH
TLLIMLHFFDAPQVSEWGWRVAFGLGGIGALVVFWLRRTMDESLSSESIADSRTGKAKAS
HHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCC
GSMRELFVNQWRPLLLCFLITAGGTIAFYTYSVTGPKMIQAAFAGGDVMAGTVINLVALT
CHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHH
VLMLMQPVGGWLSDILGRKTLLVFFGIGGVLYTWFLVMELPKQTDWLTAFAILTVGFVIL
HHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
TGYTSINALVKAELFPAHVRALGVGLGYALANSAFGGTAPLLYQASLKTNHVTEFVIYAT
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHH
AVITVSLVVYMVFLTNKGSNWLDDEAAMHQRKR
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]