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The map label for this gene is yfjR [H]

Identifier: 84625751

GI number: 84625751

Start: 4617707

End: 4618585

Strand: Reverse

Name: yfjR [H]

Synonym: XOO_4094

Alternate gene names: 84625751

Gene position: 4618585-4617707 (Counterclockwise)

Preceding gene: 84625752

Following gene: 84625750

Centisome position: 93.49

GC content: 68.03

Gene sequence:

>879_bases
ATGCCGATAGGATTTCTGGGCTTGGGCACGATGGGCCTGCCGATGGCGCACAACCTGCTGCGTGGCGGGTTCGAACTGAG
CGTTTGGAACCGTTCGCCCGAACGCGCCCAGCCGCTACGCGACGCTGGCGCCACGGTGGTGGAGCAACCGTGCGGCGCGG
CGCACGGCCCGTTGTTGTTTTCGATGCTGGCCGACGACGACGCAGTGCGCCAGACCCTGTTGGAGCGCGGCGTGCTGGAC
GCGCTGCCGGCGGGCAGCGTGCACGTCAACATGGCCACCATTTCGGTGGCGCTGGCGCATGCGTTGACCGCGCTGCATGC
CGAGCGCGGTGTGGCCTATGTGGCCGCGCCGGTGCTTGGGCGCGTGGATGTGGCCGAGGCCGGCAAGCTCAATATTCTGG
CCGGCGGCGATGACGTCGCAGTGGCACGCGTGCAGCCGATGTTCGATGCATTGGGGCAAAAGACCTGGCATATCGGTCGC
GCACCGGAGCAGGCCAATGCGGCGAAGCTGGCCGCCAACGTCTGCCTGGCCAGCGCCATCGGTGCCATGGCCGAAGCCAG
CGCACTGGCACGTGGTCACGGCGTGGATACCACGCAGTTTCTGGGCATGCTGACCAGCACCTTGTTCGCAGCGCCCGCCT
ACCAGGGCTATGGCCGCCTGATCATGCAACGCGCGTACTTGCCGGCAGGCTTCACTGCCACGCTCGGTCGCAAGGATGTG
GACCTGGCCATCCAGGCCGGCGCCGACAAACAGGTACCGATGCCGTTGGGCGAAGTGTTGCGCGCCGGCCTGGACGAGGC
CATCGCGCATGGCGACGGCCATGCAGACTGGGCGGTACTGGCGGAAGTCTCGGCGCGGCGGGCAGGGCAGGTGGCCTGA

Upstream 100 bases:

>100_bases
ACGCCGGCACCGCAATACCTGCGCTGCGAACCGCGCGACGCGCGCGGCGTGTTCGCCAACTGATCCGTACCCCCGACGTC
ATACCGAACAAGGACAACGC

Downstream 100 bases:

>100_bases
TCACCGCACAGCGGATTTACAGCGGCTAACACAACGTAGCCAGCAGTCGTCAGGTCCGTGTGGACGGCGCGGAGAAATCG
CAATGTACGAGCGGGACATG

Product: dehydrogenase

Products: (R)-Glycerate; Nicotinamide adenine dinucleotide [C]

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MPIGFLGLGTMGLPMAHNLLRGGFELSVWNRSPERAQPLRDAGATVVEQPCGAAHGPLLFSMLADDDAVRQTLLERGVLD
ALPAGSVHVNMATISVALAHALTALHAERGVAYVAAPVLGRVDVAEAGKLNILAGGDDVAVARVQPMFDALGQKTWHIGR
APEQANAAKLAANVCLASAIGAMAEASALARGHGVDTTQFLGMLTSTLFAAPAYQGYGRLIMQRAYLPAGFTATLGRKDV
DLAIQAGADKQVPMPLGEVLRAGLDEAIAHGDGHADWAVLAEVSARRAGQVA

Sequences:

>Translated_292_residues
MPIGFLGLGTMGLPMAHNLLRGGFELSVWNRSPERAQPLRDAGATVVEQPCGAAHGPLLFSMLADDDAVRQTLLERGVLD
ALPAGSVHVNMATISVALAHALTALHAERGVAYVAAPVLGRVDVAEAGKLNILAGGDDVAVARVQPMFDALGQKTWHIGR
APEQANAAKLAANVCLASAIGAMAEASALARGHGVDTTQFLGMLTSTLFAAPAYQGYGRLIMQRAYLPAGFTATLGRKDV
DLAIQAGADKQVPMPLGEVLRAGLDEAIAHGDGHADWAVLAEVSARRAGQVA
>Mature_291_residues
PIGFLGLGTMGLPMAHNLLRGGFELSVWNRSPERAQPLRDAGATVVEQPCGAAHGPLLFSMLADDDAVRQTLLERGVLDA
LPAGSVHVNMATISVALAHALTALHAERGVAYVAAPVLGRVDVAEAGKLNILAGGDDVAVARVQPMFDALGQKTWHIGRA
PEQANAAKLAANVCLASAIGAMAEASALARGHGVDTTQFLGMLTSTLFAAPAYQGYGRLIMQRAYLPAGFTATLGRKDVD
LAIQAGADKQVPMPLGEVLRAGLDEAIAHGDGHADWAVLAEVSARRAGQVA

Specific function: Glyoxylate catabolism. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=285, Percent_Identity=28.0701754385965, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI40556376, Length=258, Percent_Identity=30.2325581395349, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1786719, Length=284, Percent_Identity=29.9295774647887, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790315, Length=294, Percent_Identity=31.2925170068027, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI145693186, Length=283, Percent_Identity=28.9752650176678, Blast_Score=99, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17557316, Length=270, Percent_Identity=27.7777777777778, Blast_Score=78, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24655230, Length=295, Percent_Identity=27.1186440677966, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI19922568, Length=295, Percent_Identity=27.1186440677966, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI28574115, Length=269, Percent_Identity=25.6505576208178, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: 1.1.1.60 [C]

Molecular weight: Translated: 30200; Mature: 30069

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIGFLGLGTMGLPMAHNLLRGGFELSVWNRSPERAQPLRDAGATVVEQPCGAAHGPLLF
CCCCEEECCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCHHHHCCCCCCCHHHHH
SMLADDDAVRQTLLERGVLDALPAGSVHVNMATISVALAHALTALHAERGVAYVAAPVLG
HHHCCCHHHHHHHHHCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEHHHHHC
RVDVAEAGKLNILAGGDDVAVARVQPMFDALGQKTWHIGRAPEQANAAKLAANVCLASAI
CCCCCCCCCEEEEECCCCEEHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHH
GAMAEASALARGHGVDTTQFLGMLTSTLFAAPAYQGYGRLIMQRAYLPAGFTATLGRKDV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHCCCCCC
DLAIQAGADKQVPMPLGEVLRAGLDEAIAHGDGHADWAVLAEVSARRAGQVA
EEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PIGFLGLGTMGLPMAHNLLRGGFELSVWNRSPERAQPLRDAGATVVEQPCGAAHGPLLF
CCCEEECCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCHHHHCCCCCCCHHHHH
SMLADDDAVRQTLLERGVLDALPAGSVHVNMATISVALAHALTALHAERGVAYVAAPVLG
HHHCCCHHHHHHHHHCCHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCEEHHHHHC
RVDVAEAGKLNILAGGDDVAVARVQPMFDALGQKTWHIGRAPEQANAAKLAANVCLASAI
CCCCCCCCCEEEEECCCCEEHHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHHHHHH
GAMAEASALARGHGVDTTQFLGMLTSTLFAAPAYQGYGRLIMQRAYLPAGFTATLGRKDV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHCCCCCC
DLAIQAGADKQVPMPLGEVLRAGLDEAIAHGDGHADWAVLAEVSARRAGQVA
EEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Hydroxy-3-oxopropanoate; H+; Nicotinamide adenine dinucleotide - reduced [C]

Specific reaction: 2-Hydroxy-3-oxopropanoate + H+ + Nicotinamide adenine dinucleotide - reduced --> (R)-Glycerate + Nicotinamide adenine dinucleotide [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]