Click here to switch to the map view.

The map label for this gene is thyA [H]

Identifier: 84625200

GI number: 84625200

Start: 4004176

End: 4004970

Strand: Reverse

Name: thyA [H]

Synonym: XOO_3543

Alternate gene names: 84625200

Gene position: 4004970-4004176 (Counterclockwise)

Preceding gene: 84625201

Following gene: 84625199

Centisome position: 81.07

GC content: 61.89

Gene sequence:

>795_bases
GTGAAGCCGTATCTTGATCTGCTGCAGCATGTGCTGGAACACGGTGCGGAGAAGTCCGACCGTACCGGCACCGGCACGCG
CAGCGTGTTCGGCTGGCAGATGCGCTTCGATCTCAACGACGGCTTTCCGTTGGTGACTACCAAGAAGCTGCATCTGCGCT
CGATCATCCACGAGTTGCTGTGGTTCCTGCAGGGCGACACCAATATCGGCTATCTGAGCGACAACCAGGTGCGCATCTGG
GACGAGTGGGCCGACGACAACGGCGATCTCGGTCCGGTCTATGGCAAGCAGTGGCGGCGCTGGACCGGCCCGGATGGTGT
CGAGATCGACCAGATGCAATGGCTGGTGGACGAGATCAAGCGCAACCCGGATTCGCGCCGGCTGGTGATCAGCGCCTGGA
ATGTCGGCGAGCTGCCGCAGATGGCGCTGATGCCGTGCCACAGCCTGTTCCAGTTCTACGTGGTGAACGGCAAGCTCAGT
TGCCAGCTCTACCAGCGCAGCGGCGACATCTTTCTGGGCGTGCCGTTCAATATCGCCAGCTACGCGTTGCTCACGCACAT
GGTGGCGCAGGCCACCGGCCTGGGCGTGGGCGATTTCGTGCATACGTTGGGCGATGCGCACCTGTATTCGAATCACTTCG
ATCAAGCGCGTAAGCAACTGACGCGTACACCGCGTGCGCTGCCCACCCTGCGCTTGAATCCCGAGGTCACCGACCTGTTC
GCGTTCCGCTTCGAAGACATCGCCATCGAAGGTTACGATCCGCATCCGGCGATCAAGGCGCCGGTGGCGGTCTGA

Upstream 100 bases:

>100_bases
TGCCGCTGATTGCGGTTGGCCTGGCGCTGCTGGCGATGTCGCGCCGCGCGCCGGTGTTGCAGCCGGTGCTGCCGACGCCC
GCTGGCGTGGAGGCTGCCAA

Downstream 100 bases:

>100_bases
CTCCTGGTCTCGATGCATGAGACGCAGGCGATGCATGATGATTGCTCACCTGGAAGGAGGTGTGTGATGGCTAAGTACTC
GATTAAAGCAAGAGAAAAAC

Product: thymidylate synthase

Products: NA

Alternate protein names: TS; TSase [H]

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKPYLDLLQHVLEHGAEKSDRTGTGTRSVFGWQMRFDLNDGFPLVTTKKLHLRSIIHELLWFLQGDTNIGYLSDNQVRIW
DEWADDNGDLGPVYGKQWRRWTGPDGVEIDQMQWLVDEIKRNPDSRRLVISAWNVGELPQMALMPCHSLFQFYVVNGKLS
CQLYQRSGDIFLGVPFNIASYALLTHMVAQATGLGVGDFVHTLGDAHLYSNHFDQARKQLTRTPRALPTLRLNPEVTDLF
AFRFEDIAIEGYDPHPAIKAPVAV

Sequences:

>Translated_264_residues
MKPYLDLLQHVLEHGAEKSDRTGTGTRSVFGWQMRFDLNDGFPLVTTKKLHLRSIIHELLWFLQGDTNIGYLSDNQVRIW
DEWADDNGDLGPVYGKQWRRWTGPDGVEIDQMQWLVDEIKRNPDSRRLVISAWNVGELPQMALMPCHSLFQFYVVNGKLS
CQLYQRSGDIFLGVPFNIASYALLTHMVAQATGLGVGDFVHTLGDAHLYSNHFDQARKQLTRTPRALPTLRLNPEVTDLF
AFRFEDIAIEGYDPHPAIKAPVAV
>Mature_264_residues
MKPYLDLLQHVLEHGAEKSDRTGTGTRSVFGWQMRFDLNDGFPLVTTKKLHLRSIIHELLWFLQGDTNIGYLSDNQVRIW
DEWADDNGDLGPVYGKQWRRWTGPDGVEIDQMQWLVDEIKRNPDSRRLVISAWNVGELPQMALMPCHSLFQFYVVNGKLS
CQLYQRSGDIFLGVPFNIASYALLTHMVAQATGLGVGDFVHTLGDAHLYSNHFDQARKQLTRTPRALPTLRLNPEVTDLF
AFRFEDIAIEGYDPHPAIKAPVAV

Specific function: Provides the sole de novo source of dTMP for DNA biosynthesis [H]

COG id: COG0207

COG function: function code F; Thymidylate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate synthase family. Bacterial- type thyA subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507751, Length=281, Percent_Identity=53.0249110320285, Blast_Score=298, Evalue=4e-81,
Organism=Escherichia coli, GI1789191, Length=264, Percent_Identity=68.9393939393939, Blast_Score=391, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71993377, Length=282, Percent_Identity=46.8085106382979, Blast_Score=264, Evalue=4e-71,
Organism=Saccharomyces cerevisiae, GI6324648, Length=290, Percent_Identity=38.6206896551724, Blast_Score=191, Evalue=1e-49,
Organism=Drosophila melanogaster, GI17137556, Length=283, Percent_Identity=51.2367491166078, Blast_Score=287, Evalue=6e-78,

Paralogues:

None

Copy number: 391 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000398
- InterPro:   IPR020940 [H]

Pfam domain/function: PF00303 Thymidylat_synt [H]

EC number: =2.1.1.45 [H]

Molecular weight: Translated: 30106; Mature: 30106

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00091 THYMIDYLATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPYLDLLQHVLEHGAEKSDRTGTGTRSVFGWQMRFDLNDGFPLVTTKKLHLRSIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHH
WFLQGDTNIGYLSDNQVRIWDEWADDNGDLGPVYGKQWRRWTGPDGVEIDQMQWLVDEIK
HHHCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RNPDSRRLVISAWNVGELPQMALMPCHSLFQFYVVNGKLSCQLYQRSGDIFLGVPFNIAS
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEEECCEEEEEEEECCCCEEEECCHHHHH
YALLTHMVAQATGLGVGDFVHTLGDAHLYSNHFDQARKQLTRTPRALPTLRLNPEVTDLF
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHH
AFRFEDIAIEGYDPHPAIKAPVAV
HEEEEEEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MKPYLDLLQHVLEHGAEKSDRTGTGTRSVFGWQMRFDLNDGFPLVTTKKLHLRSIIHELL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECCCCCCEEEEHHHHHHHHHHHHH
WFLQGDTNIGYLSDNQVRIWDEWADDNGDLGPVYGKQWRRWTGPDGVEIDQMQWLVDEIK
HHHCCCCCCEEECCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
RNPDSRRLVISAWNVGELPQMALMPCHSLFQFYVVNGKLSCQLYQRSGDIFLGVPFNIAS
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHEEEECCEEEEEEEECCCCEEEECCHHHHH
YALLTHMVAQATGLGVGDFVHTLGDAHLYSNHFDQARKQLTRTPRALPTLRLNPEVTDLF
HHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHH
AFRFEDIAIEGYDPHPAIKAPVAV
HEEEEEEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]