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The map label for this gene is purF [H]

Identifier: 84625129

GI number: 84625129

Start: 3931691

End: 3933157

Strand: Reverse

Name: purF [H]

Synonym: XOO_3472

Alternate gene names: 84625129

Gene position: 3933157-3931691 (Counterclockwise)

Preceding gene: 84625130

Following gene: 84625128

Centisome position: 79.62

GC content: 63.39

Gene sequence:

>1467_bases
ATGTGTGGCATCGTCGGTATTGTCGGCAACCAGAACGTCGCCGGGCAGCTGTATGACGGCCTGACCGTCCTGCAGCATCG
TGGGCAGGACGCCGCAGGCATCGCCACCGCAGATGGCACGCGCCTGCGGGTGCAGAAGGCCAATGGCCTGGTCCGCGACG
TCTTCGACGAAAAGAAAATGGCGGTGCTGGAAGGCCGCGTCGGTATCGCGCATTGCCGTTACCCGACCGCAGGTTCGGAA
GGCATGGACGAGGCGCAGCCGTTCTATGTCAACTCGCCTTACGGCATCGCGCTGGCGCACAACGGCAACCTGATCAACAC
CGAGGCCTTGCGCCAGCAGGTGTTCGAAGCCGACCGCCGCAACATCAATACCGATTCGGACAGCGAAGTGCTGTTGAACG
TGTTCGCCTACGAGCTGGACGCGCAGCGCATGCTTACCCCCGAAGCGGCCATCCGCGCGGTGGCCGGCGTGCACCGCCGC
TGCAAGGGCGGCTACGCGGTGGTCAGCGTGGTGTTGGGCCTAGGCCTGGTCGCGTTCCGCGATCCGCACGGCATCCGCCC
GCTGGTGCTGGGCAAGCGCGAGCATGCCGAAGGCGCCGAATACATCGTGTCTTCCGAATCGGCAGCGCTGGACATCCTGG
GTTATCAGCGCGTGCGCGACGTGCGCCCGGGCGAAGCGTTGGTGATTACGGCGCGCGGCGAGCTGTTTTCGGAAATCTGC
GCTTCGCCGACCAACAACGCGCCGTGCATTTTCGAGTACGTGTATTTCGCCCGTCCCGATTCGATGATCGACAACATCTC
GGTGCACAAGGCGCGCATGCGCATGGGCCTGAAGCTGGGCGAAAAGATCCTGCGCCTGCGCCCGGATCATGATATCGACA
CCATCATTCCGATCCCGGACACCTCGCGCGATGTGGCGCTGGAGATGTCCAACGTGCTCGGCGTGAAGTACCGCGAGGGC
TTCGTCAAGAACCGTTACGTGGGCCGCACCTTCATCATGCCGGGGCAGGGCGAACGGCAGAAATCGGTACGCCGCAAGCT
CAACCCGATCCACCTGGAATTCCGCAACCGTGTGGTGTTGCTGGTCGACGATTCGATCGTGCGCGGCACCACCAGCCGGC
AGATCGTGCAGATGGCGCGCGACGCCGGCGCGCGCAAGGTGTACCTGGCCTCGGCTGCGCCGCCGGTGCGTTACCCAAAC
ATCTACGGCATCGACATGCCGGCGGCCGAAGAGCTCATTGCGCATGGCCGCAGCGAGTTGGAAATCCAGGAGTTTCTGGG
CTGCGACTGGCTGATCTACCAGGATCTGGAAGACCTGGAAGTGGCGGTGCGCGAAGGGAATCCGGACATCAAGCAGTTCG
ACTCTTCGTGCTTCAACGGCGAGTACATCACCGGCATCGAACCGGGATATTTCGAACGCATCCAGCAGCTGCGCTCAGAC
GATGCCAAGAAGCGTCGTCGCGCCTGA

Upstream 100 bases:

>100_bases
GGTTCGCGGCGGAGAAAGCGACCCGGCTCGGGTCGAGTCCCCAGGCCAGGCACGGCCACCTTCACAGTAACGGCGCAAGC
CGAAGCGGAGAACGCGCACC

Downstream 100 bases:

>100_bases
ATTACCGCGGCGACGCGGCCGATTGGTCGCGCGCGCCGGGCTGGCGCTGCCGGCTGGCTGTGCAAGGCAAAACGCGTGTA
AGTGCATGCCGGTGCGCTGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MCGIVGIVGNQNVAGQLYDGLTVLQHRGQDAAGIATADGTRLRVQKANGLVRDVFDEKKMAVLEGRVGIAHCRYPTAGSE
GMDEAQPFYVNSPYGIALAHNGNLINTEALRQQVFEADRRNINTDSDSEVLLNVFAYELDAQRMLTPEAAIRAVAGVHRR
CKGGYAVVSVVLGLGLVAFRDPHGIRPLVLGKREHAEGAEYIVSSESAALDILGYQRVRDVRPGEALVITARGELFSEIC
ASPTNNAPCIFEYVYFARPDSMIDNISVHKARMRMGLKLGEKILRLRPDHDIDTIIPIPDTSRDVALEMSNVLGVKYREG
FVKNRYVGRTFIMPGQGERQKSVRRKLNPIHLEFRNRVVLLVDDSIVRGTTSRQIVQMARDAGARKVYLASAAPPVRYPN
IYGIDMPAAEELIAHGRSELEIQEFLGCDWLIYQDLEDLEVAVREGNPDIKQFDSSCFNGEYITGIEPGYFERIQQLRSD
DAKKRRRA

Sequences:

>Translated_488_residues
MCGIVGIVGNQNVAGQLYDGLTVLQHRGQDAAGIATADGTRLRVQKANGLVRDVFDEKKMAVLEGRVGIAHCRYPTAGSE
GMDEAQPFYVNSPYGIALAHNGNLINTEALRQQVFEADRRNINTDSDSEVLLNVFAYELDAQRMLTPEAAIRAVAGVHRR
CKGGYAVVSVVLGLGLVAFRDPHGIRPLVLGKREHAEGAEYIVSSESAALDILGYQRVRDVRPGEALVITARGELFSEIC
ASPTNNAPCIFEYVYFARPDSMIDNISVHKARMRMGLKLGEKILRLRPDHDIDTIIPIPDTSRDVALEMSNVLGVKYREG
FVKNRYVGRTFIMPGQGERQKSVRRKLNPIHLEFRNRVVLLVDDSIVRGTTSRQIVQMARDAGARKVYLASAAPPVRYPN
IYGIDMPAAEELIAHGRSELEIQEFLGCDWLIYQDLEDLEVAVREGNPDIKQFDSSCFNGEYITGIEPGYFERIQQLRSD
DAKKRRRA
>Mature_488_residues
MCGIVGIVGNQNVAGQLYDGLTVLQHRGQDAAGIATADGTRLRVQKANGLVRDVFDEKKMAVLEGRVGIAHCRYPTAGSE
GMDEAQPFYVNSPYGIALAHNGNLINTEALRQQVFEADRRNINTDSDSEVLLNVFAYELDAQRMLTPEAAIRAVAGVHRR
CKGGYAVVSVVLGLGLVAFRDPHGIRPLVLGKREHAEGAEYIVSSESAALDILGYQRVRDVRPGEALVITARGELFSEIC
ASPTNNAPCIFEYVYFARPDSMIDNISVHKARMRMGLKLGEKILRLRPDHDIDTIIPIPDTSRDVALEMSNVLGVKYREG
FVKNRYVGRTFIMPGQGERQKSVRRKLNPIHLEFRNRVVLLVDDSIVRGTTSRQIVQMARDAGARKVYLASAAPPVRYPN
IYGIDMPAAEELIAHGRSELEIQEFLGCDWLIYQDLEDLEVAVREGNPDIKQFDSSCFNGEYITGIEPGYFERIQQLRSD
DAKKRRRA

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=469, Percent_Identity=34.9680170575693, Blast_Score=266, Evalue=4e-71,
Organism=Escherichia coli, GI1788651, Length=492, Percent_Identity=61.9918699186992, Blast_Score=602, Evalue=1e-173,
Organism=Escherichia coli, GI1790167, Length=172, Percent_Identity=33.1395348837209, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=487, Percent_Identity=35.3182751540041, Blast_Score=243, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6323958, Length=491, Percent_Identity=53.5641547861507, Blast_Score=506, Evalue=1e-144,
Organism=Drosophila melanogaster, GI28573187, Length=495, Percent_Identity=34.5454545454545, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24659598, Length=485, Percent_Identity=35.0515463917526, Blast_Score=252, Evalue=4e-67,
Organism=Drosophila melanogaster, GI24659604, Length=477, Percent_Identity=35.4297693920335, Blast_Score=250, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54271; Mature: 54271

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGIVGNQNVAGQLYDGLTVLQHRGQDAAGIATADGTRLRVQKANGLVRDVFDEKKM
CCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCHHHHHHHHHHH
AVLEGRVGIAHCRYPTAGSEGMDEAQPFYVNSPYGIALAHNGNLINTEALRQQVFEADRR
HHHCCCCEEEEECCCCCCCCCCCCCCCEEEECCCEEEEEECCCEECHHHHHHHHHHHHHC
NINTDSDSEVLLNVFAYELDAQRMLTPEAAIRAVAGVHRRCKGGYAVVSVVLGLGLVAFR
CCCCCCCHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEC
DPHGIRPLVLGKREHAEGAEYIVSSESAALDILGYQRVRDVRPGEALVITARGELFSEIC
CCCCCCCEEECCCCCCCCCCEEEECCCCEEEHHHHHHHHCCCCCCEEEEEECHHHHHHHH
ASPTNNAPCIFEYVYFARPDSMIDNISVHKARMRMGLKLGEKILRLRPDHDIDTIIPIPD
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
TSRDVALEMSNVLGVKYREGFVKNRYVGRTFIMPGQGERQKSVRRKLNPIHLEFRNRVVL
CCCHHHEEHHHHHCCHHHHCCHHCCCCCEEEEECCCCHHHHHHHHCCCCEEEEECCEEEE
LVDDSIVRGTTSRQIVQMARDAGARKVYLASAAPPVRYPNIYGIDMPAAEELIAHGRSEL
EECCCHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCHHHHHHHCCCCCE
EIQEFLGCDWLIYQDLEDLEVAVREGNPDIKQFDSSCFNGEYITGIEPGYFERIQQLRSD
EHHHHHCCCEEEECCHHHHHHHHHCCCCCHHHHHHHCCCCCEEECCCCHHHHHHHHHHCC
DAKKRRRA
HHHHHHCC
>Mature Secondary Structure
MCGIVGIVGNQNVAGQLYDGLTVLQHRGQDAAGIATADGTRLRVQKANGLVRDVFDEKKM
CCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCHHHHHHHHHHH
AVLEGRVGIAHCRYPTAGSEGMDEAQPFYVNSPYGIALAHNGNLINTEALRQQVFEADRR
HHHCCCCEEEEECCCCCCCCCCCCCCCEEEECCCEEEEEECCCEECHHHHHHHHHHHHHC
NINTDSDSEVLLNVFAYELDAQRMLTPEAAIRAVAGVHRRCKGGYAVVSVVLGLGLVAFR
CCCCCCCHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHEEEC
DPHGIRPLVLGKREHAEGAEYIVSSESAALDILGYQRVRDVRPGEALVITARGELFSEIC
CCCCCCCEEECCCCCCCCCCEEEECCCCEEEHHHHHHHHCCCCCCEEEEEECHHHHHHHH
ASPTNNAPCIFEYVYFARPDSMIDNISVHKARMRMGLKLGEKILRLRPDHDIDTIIPIPD
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
TSRDVALEMSNVLGVKYREGFVKNRYVGRTFIMPGQGERQKSVRRKLNPIHLEFRNRVVL
CCCHHHEEHHHHHCCHHHHCCHHCCCCCEEEEECCCCHHHHHHHHCCCCEEEEECCEEEE
LVDDSIVRGTTSRQIVQMARDAGARKVYLASAAPPVRYPNIYGIDMPAAEELIAHGRSEL
EECCCHHCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCHHHHHHHCCCCCE
EIQEFLGCDWLIYQDLEDLEVAVREGNPDIKQFDSSCFNGEYITGIEPGYFERIQQLRSD
EHHHHHCCCEEEECCHHHHHHHHHCCCCCHHHHHHHCCCCCEEECCCCHHHHHHHHHHCC
DAKKRRRA
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]