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The map label for this gene is yfiK [H]

Identifier: 84624990

GI number: 84624990

Start: 3795657

End: 3796298

Strand: Reverse

Name: yfiK [H]

Synonym: XOO_3333

Alternate gene names: 84624990

Gene position: 3796298-3795657 (Counterclockwise)

Preceding gene: 84624991

Following gene: 84624989

Centisome position: 76.84

GC content: 64.8

Gene sequence:

>642_bases
ATGATCCGTGTGTGCCTGGTCGACGATCAAACCCTGGTGCGCCAGGGGATCCGCTCGCTGTTGGCGCTGGACGACGGGAT
CGAAGTGGTGGCCGAGGCCTCCGACGGCAAGCAGGCGGTGGAGCAGATTCCGCAGATCCAGCCGGACGTGGTGCTGATGG
ACATGCGCATGCCGGTGATGTCCGGCCTGGAAGCGCTGCAGATGCTCTCGCGTAACGGCACGCTGCCGCCGACGATCATC
CTGACCACCTTCGACGACGACCAGCTGGTGCTGGCCGGGCTCAAGGCCGGCGCCAAGGGCTATCTGCTCAAGGACGTCTC
GCTGGAACAGCTGGTTGGCGCCATTCGTACCGTGGCCGACGGCGGCTCGCTGGTGCAGCCGGCGGTGACCCAGCGCCTGC
TGTCGGGCCTGGAACATATGCGCAACGAGTTCGTCAGCCTGGATCGGCCCGATCCGCTCACCGACCGCGAGACCGAGATC
CTGCGGTTGATGGCGAGCGGATTTTCCAACAAGGAAATCGCCAACTCGCTGGGCGTGGCCGAGGGCACCATCAAGAACCA
CGTCTCCAACATTCTCTCCAAGCTCGGCGTACGCGACCGCACTCGCGCGGTGCTGAAGGCGTTCGAGTTGCAGTTGGTGT
GA

Upstream 100 bases:

>100_bases
TTGAGATCCAAACCCGGCGGGGTGACGGCTTCGGCCTGCGCATCGCTGTTCCAGGCGCGCCGGCCCTGATGCCTGCGGCC
GTGACCCAAGGAGTGTTGTG

Downstream 100 bases:

>100_bases
GGTTGGCGCGCCGCCGCTGACCTCGATGTGCGCGTGCCGGCGGCAGGGCGGGGAATCGCATTCAGGGCGCCGCCGCCCCG
TTCAAGCCGCGCTATGGGGC

Product: two-component system regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MIRVCLVDDQTLVRQGIRSLLALDDGIEVVAEASDGKQAVEQIPQIQPDVVLMDMRMPVMSGLEALQMLSRNGTLPPTII
LTTFDDDQLVLAGLKAGAKGYLLKDVSLEQLVGAIRTVADGGSLVQPAVTQRLLSGLEHMRNEFVSLDRPDPLTDRETEI
LRLMASGFSNKEIANSLGVAEGTIKNHVSNILSKLGVRDRTRAVLKAFELQLV

Sequences:

>Translated_213_residues
MIRVCLVDDQTLVRQGIRSLLALDDGIEVVAEASDGKQAVEQIPQIQPDVVLMDMRMPVMSGLEALQMLSRNGTLPPTII
LTTFDDDQLVLAGLKAGAKGYLLKDVSLEQLVGAIRTVADGGSLVQPAVTQRLLSGLEHMRNEFVSLDRPDPLTDRETEI
LRLMASGFSNKEIANSLGVAEGTIKNHVSNILSKLGVRDRTRAVLKAFELQLV
>Mature_213_residues
MIRVCLVDDQTLVRQGIRSLLALDDGIEVVAEASDGKQAVEQIPQIQPDVVLMDMRMPVMSGLEALQMLSRNGTLPPTII
LTTFDDDQLVLAGLKAGAKGYLLKDVSLEQLVGAIRTVADGGSLVQPAVTQRLLSGLEHMRNEFVSLDRPDPLTDRETEI
LRLMASGFSNKEIANSLGVAEGTIKNHVSNILSKLGVRDRTRAVLKAFELQLV

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=205, Percent_Identity=43.4146341463415, Blast_Score=144, Evalue=4e-36,
Organism=Escherichia coli, GI1790102, Length=209, Percent_Identity=36.8421052631579, Blast_Score=127, Evalue=4e-31,
Organism=Escherichia coli, GI1788222, Length=201, Percent_Identity=36.81592039801, Blast_Score=110, Evalue=7e-26,
Organism=Escherichia coli, GI1787473, Length=186, Percent_Identity=36.5591397849462, Blast_Score=100, Evalue=9e-23,
Organism=Escherichia coli, GI1788712, Length=193, Percent_Identity=25.9067357512953, Blast_Score=81, Evalue=6e-17,
Organism=Escherichia coli, GI1788550, Length=109, Percent_Identity=35.7798165137615, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1786747, Length=200, Percent_Identity=26.5, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87082052, Length=119, Percent_Identity=33.6134453781513, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI2367223, Length=153, Percent_Identity=33.3333333333333, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1788546, Length=201, Percent_Identity=27.363184079602, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23154; Mature: 23154

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRVCLVDDQTLVRQGIRSLLALDDGIEVVAEASDGKQAVEQIPQIQPDVVLMDMRMPVM
CEEEEEECCHHHHHHHHHHHHHHCCCHHHEEECCCCHHHHHHCCCCCCCEEEEECCCHHH
SGLEALQMLSRNGTLPPTIILTTFDDDQLVLAGLKAGAKGYLLKDVSLEQLVGAIRTVAD
HHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEHHCCCCCEEECCCCHHHHHHHHHHHHC
GGSLVQPAVTQRLLSGLEHMRNEFVSLDRPDPLTDRETEILRLMASGFSNKEIANSLGVA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCC
EGTIKNHVSNILSKLGVRDRTRAVLKAFELQLV
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIRVCLVDDQTLVRQGIRSLLALDDGIEVVAEASDGKQAVEQIPQIQPDVVLMDMRMPVM
CEEEEEECCHHHHHHHHHHHHHHCCCHHHEEECCCCHHHHHHCCCCCCCEEEEECCCHHH
SGLEALQMLSRNGTLPPTIILTTFDDDQLVLAGLKAGAKGYLLKDVSLEQLVGAIRTVAD
HHHHHHHHHHCCCCCCCEEEEEECCCCCEEEEEHHCCCCCEEECCCCHHHHHHHHHHHHC
GGSLVQPAVTQRLLSGLEHMRNEFVSLDRPDPLTDRETEILRLMASGFSNKEIANSLGVA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHCCC
EGTIKNHVSNILSKLGVRDRTRAVLKAFELQLV
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]