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The map label for this gene is purT [H]

Identifier: 84624982

GI number: 84624982

Start: 3789709

End: 3790911

Strand: Reverse

Name: purT [H]

Synonym: XOO_3325

Alternate gene names: 84624982

Gene position: 3790911-3789709 (Counterclockwise)

Preceding gene: 84624987

Following gene: 84624979

Centisome position: 76.74

GC content: 66.83

Gene sequence:

>1203_bases
ATGACCACCCTTGGCACCCCGCTGTCGCCTTCCGCCACCCGCGTGCTGCTGCTGGGCTCTGGTGAGCTGGGCAAGGAAGT
GGCGATTGAACTGCAGCGTTTCGGCGTGGAAGTGATCGCGGCCGACCGCTACGCCAATGCGCCGGCGATGCAGGTGGCGC
ATCGCTCGCACGTGCTGGATATGCTCGACCCCGCAGCGCTGCGCGTGCTGATCGCCAGCGAGCGGCCGCATGTGATCGTG
CCGGAGATCGAAGCGATCCATACCGAAACACTGGTGGCACTGGAACGCGAGCAGGGCCAGAAAGTCATCCCCGCTGCGCG
TGCCGCACGCCTGACCATGGACCGCGAAGGCATCCGTCGGCTGGCTGCCGAAACGCTGGGCCTGCCGACCTCGCCGTATC
GCTTCGTCGACACCGCTGCCGACTACCGCGAGGCGATTGCGGCAGTGGGCCTGCCATGCGTGGTCAAGCCGGTGATGTCG
TCCTCGGGCAAGGGCCAGAGCACCTTGCGCAGCGAAGCCGATATCGATGCGGCGTGGGAGTACGCGCAGACCGGAGGGCG
TGCCGGCGCCGGCCGCTGCATCGTCGAAGGCTTTATCGATTTCGATTACGAAATCACCTTGCTCACCGTGCGCCATGCCG
GTGGCACCTCGTACTGCGATCCCATCGGACATTGGCAGCAGGACGGCGACTATCGCGAAAGCTGGCAGCCGCAGCCGATG
TCGGCCGCTGCATTGCGCCGCTCGCAGGAGATCGCCAAGGCCATCACCGACGATCTCGGCGGCTGGGGCCTGTTCGGCGT
GGAATTGTTCGTCAAGGGCGACGAAGTGTGGTTCAGCGAAGTGTCGCCGCGCCCGCACGACACCGGTCTGGTCACGCTGG
TCTCGCAGGAACTGAGCGAATTCGCGCTGCACGCCCGCGCCATTCTTGGCCTGCCGGTGGGCGCAGAAGACGGCGGCGTG
ATCGGTCAGAGCGGCCCCTCCGCATCCTGCGCGTTGCTGGCGCACGGCAATGGCGTGCCGGTGTTCGACAACGTGGCCGA
CGCGCTACGCGATCCGAACACCGCATTGCGCCTGTTCGGTAAACCCCGCGTGGATGGTCATCGCCGCGTCGGTGTCACCC
TGGCGCGCGCGGACAGCATCGATGCTGCGCGCGAGAAGGCGCGTGTGGCGGCGGCTGCGCTGGGGATACAACTGACTGCC
TGA

Upstream 100 bases:

>100_bases
AAACCATATCAATATGATATCAGGCAAGCAGCAGTCGTTCGTCCGGCGTCGGCCTTGGCAGCCGTTAGAATGGCGCCCCC
CTTCGTTCTGGACTGCGCCC

Downstream 100 bases:

>100_bases
GCTTGCGGCCGACTCGCACTGGTGCAGCGGCAGGATTGCTGCATTGACCATTTAGGGCACCTCGAAGAACCAGGTCGGAG
CCGATGCAGCGCATGCATGG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTTLGTPLSPSATRVLLLGSGELGKEVAIELQRFGVEVIAADRYANAPAMQVAHRSHVLDMLDPAALRVLIASERPHVIV
PEIEAIHTETLVALEREQGQKVIPAARAARLTMDREGIRRLAAETLGLPTSPYRFVDTAADYREAIAAVGLPCVVKPVMS
SSGKGQSTLRSEADIDAAWEYAQTGGRAGAGRCIVEGFIDFDYEITLLTVRHAGGTSYCDPIGHWQQDGDYRESWQPQPM
SAAALRRSQEIAKAITDDLGGWGLFGVELFVKGDEVWFSEVSPRPHDTGLVTLVSQELSEFALHARAILGLPVGAEDGGV
IGQSGPSASCALLAHGNGVPVFDNVADALRDPNTALRLFGKPRVDGHRRVGVTLARADSIDAAREKARVAAAALGIQLTA

Sequences:

>Translated_400_residues
MTTLGTPLSPSATRVLLLGSGELGKEVAIELQRFGVEVIAADRYANAPAMQVAHRSHVLDMLDPAALRVLIASERPHVIV
PEIEAIHTETLVALEREQGQKVIPAARAARLTMDREGIRRLAAETLGLPTSPYRFVDTAADYREAIAAVGLPCVVKPVMS
SSGKGQSTLRSEADIDAAWEYAQTGGRAGAGRCIVEGFIDFDYEITLLTVRHAGGTSYCDPIGHWQQDGDYRESWQPQPM
SAAALRRSQEIAKAITDDLGGWGLFGVELFVKGDEVWFSEVSPRPHDTGLVTLVSQELSEFALHARAILGLPVGAEDGGV
IGQSGPSASCALLAHGNGVPVFDNVADALRDPNTALRLFGKPRVDGHRRVGVTLARADSIDAAREKARVAAAALGIQLTA
>Mature_399_residues
TTLGTPLSPSATRVLLLGSGELGKEVAIELQRFGVEVIAADRYANAPAMQVAHRSHVLDMLDPAALRVLIASERPHVIVP
EIEAIHTETLVALEREQGQKVIPAARAARLTMDREGIRRLAAETLGLPTSPYRFVDTAADYREAIAAVGLPCVVKPVMSS
SGKGQSTLRSEADIDAAWEYAQTGGRAGAGRCIVEGFIDFDYEITLLTVRHAGGTSYCDPIGHWQQDGDYRESWQPQPMS
AAALRRSQEIAKAITDDLGGWGLFGVELFVKGDEVWFSEVSPRPHDTGLVTLVSQELSEFALHARAILGLPVGAEDGGVI
GQSGPSASCALLAHGNGVPVFDNVADALRDPNTALRLFGKPRVDGHRRVGVTLARADSIDAAREKARVAAAALGIQLTA

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=398, Percent_Identity=63.8190954773869, Blast_Score=498, Evalue=1e-142,
Organism=Escherichia coli, GI1786733, Length=192, Percent_Identity=30.2083333333333, Blast_Score=75, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324702, Length=309, Percent_Identity=23.9482200647249, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42633; Mature: 42502

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLGTPLSPSATRVLLLGSGELGKEVAIELQRFGVEVIAADRYANAPAMQVAHRSHVLD
CCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHH
MLDPAALRVLIASERPHVIVPEIEAIHTETLVALEREQGQKVIPAARAARLTMDREGIRR
HCCHHHEEEEEECCCCCEECCCHHHHHHHHHHEEHHHCCCEECCHHHHHHHHHCHHHHHH
LAAETLGLPTSPYRFVDTAADYREAIAAVGLPCVVKPVMSSSGKGQSTLRSEADIDAAWE
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHCCCHHHHHH
YAQTGGRAGAGRCIVEGFIDFDYEITLLTVRHAGGTSYCDPIGHWQQDGDYRESWQPQPM
HHHCCCCCCCCCEEEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCC
SAAALRRSQEIAKAITDDLGGWGLFGVELFVKGDEVWFSEVSPRPHDTGLVTLVSQELSE
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHH
FALHARAILGLPVGAEDGGVIGQSGPSASCALLAHGNGVPVFDNVADALRDPNTALRLFG
HHHHHHHHHCCCCCCCCCCEECCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEC
KPRVDGHRRVGVTLARADSIDAAREKARVAAAALGIQLTA
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCEEECC
>Mature Secondary Structure 
TTLGTPLSPSATRVLLLGSGELGKEVAIELQRFGVEVIAADRYANAPAMQVAHRSHVLD
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHH
MLDPAALRVLIASERPHVIVPEIEAIHTETLVALEREQGQKVIPAARAARLTMDREGIRR
HCCHHHEEEEEECCCCCEECCCHHHHHHHHHHEEHHHCCCEECCHHHHHHHHHCHHHHHH
LAAETLGLPTSPYRFVDTAADYREAIAAVGLPCVVKPVMSSSGKGQSTLRSEADIDAAWE
HHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHCCCHHHHHH
YAQTGGRAGAGRCIVEGFIDFDYEITLLTVRHAGGTSYCDPIGHWQQDGDYRESWQPQPM
HHHCCCCCCCCCEEEEEEECCCEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHCCCCCCC
SAAALRRSQEIAKAITDDLGGWGLFGVELFVKGDEVWFSEVSPRPHDTGLVTLVSQELSE
HHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEEECCCCCCCCCCHHHHHHHHHHH
FALHARAILGLPVGAEDGGVIGQSGPSASCALLAHGNGVPVFDNVADALRDPNTALRLFG
HHHHHHHHHCCCCCCCCCCEECCCCCCCEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEC
KPRVDGHRRVGVTLARADSIDAAREKARVAAAALGIQLTA
CCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]