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The map label for this gene is cysD [H]

Identifier: 84624855

GI number: 84624855

Start: 3623762

End: 3624670

Strand: Reverse

Name: cysD [H]

Synonym: XOO_3198

Alternate gene names: 84624855

Gene position: 3624670-3623762 (Counterclockwise)

Preceding gene: 84624859

Following gene: 84624854

Centisome position: 73.37

GC content: 63.04

Gene sequence:

>909_bases
ATGACCCTGCCGCCGCTGTCCCATCTCGACCGGCTCGAAGCCGAAAGCATCCATATCCTGCGCGAAGTCGCTGCCGAATT
CCGGGCTCCGGTGATGCTGTATTCGGTGGGCAAGGACAGCTCGGTGCTGCTGCATCTGCTGCTCAAGGCATTTGCGCCGT
CGCGGCCGCCGATCCCGTTGCTGCACATCGACACACGCTGGAAGTTCCGCGAAATGATCGCCTTCCGCGACCGCCGTGCG
GCCGAAACCGGGGTGGATCTGCGTGTGCACATCAATCCTGATGGCGTCGCCCAAGACGTCAGCCCGATCAGCCACGGCGC
CGCCGTGCATACCGACATCATGAAGACCCAGGGTCTGAAACAGGCGTTGGAGCAGGGCGGCTTCGATGCGGCCATTGGCG
GAGCGCGCCGCGACGAAGAGAAATCGCGCGCCAAGGAGCGTGTGTTCTCGTTCCGCAATGCACGCCATCGCTGGGACCCG
AAGAACCAGCGCCCGGAACTGTGGAATCTCTACAACGCGCGCACCAAGCCCGGCGAAAGCGTGCGCGTGTTCCCGCTCTC
CAACTGGACCGAGCTGGATGTCTGGCTGTACATCTATCGCGAAAAAATTCCGGTGGTGCCGCTGTACTTTGCCGCCCCTC
GCCCGGTGGTGGCACGCGACGGTGCCTGGATCATGGTCGACGACGCGCGTTTGCCGTTGCATCCAGGCGAAACCCCGCAA
TTGCGTTCGGTGCGCTTCCGCACGCTGGGCTGCTACCCGCTCACCGGTGCGATCGAATCCAGTGCCGACACGCTGGAAGC
GGTCATCGCCGAAATGCTGGTCAGCACCAGCTCCGAGCGCCAAGGCCGCATGATCGATCACGCACCGGGCGCATCGATGG
AACAGAAAAAGGTTGAGGGGTATTTTTGA

Upstream 100 bases:

>100_bases
ATGCCGGCCGCTTATTTCGTCCAGTCATCGGCGCAGCACACACTGCAACGCCTTGCCCTAACCATCGGCTGGTCTGCCTG
CCGACACCCACGATAGTCCG

Downstream 100 bases:

>100_bases
TGGGCAGTGATTCGGGAGTCGGCATTCGGGATTCGCAGGAGCAATTCGCGGCGAGCGGAAAGATCGACGGGAGCGCTCTG
GCGAATCCCCACTCCCCACT

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT [H]

Number of amino acids: Translated: 302; Mature: 301

Protein sequence:

>302_residues
MTLPPLSHLDRLEAESIHILREVAAEFRAPVMLYSVGKDSSVLLHLLLKAFAPSRPPIPLLHIDTRWKFREMIAFRDRRA
AETGVDLRVHINPDGVAQDVSPISHGAAVHTDIMKTQGLKQALEQGGFDAAIGGARRDEEKSRAKERVFSFRNARHRWDP
KNQRPELWNLYNARTKPGESVRVFPLSNWTELDVWLYIYREKIPVVPLYFAAPRPVVARDGAWIMVDDARLPLHPGETPQ
LRSVRFRTLGCYPLTGAIESSADTLEAVIAEMLVSTSSERQGRMIDHAPGASMEQKKVEGYF

Sequences:

>Translated_302_residues
MTLPPLSHLDRLEAESIHILREVAAEFRAPVMLYSVGKDSSVLLHLLLKAFAPSRPPIPLLHIDTRWKFREMIAFRDRRA
AETGVDLRVHINPDGVAQDVSPISHGAAVHTDIMKTQGLKQALEQGGFDAAIGGARRDEEKSRAKERVFSFRNARHRWDP
KNQRPELWNLYNARTKPGESVRVFPLSNWTELDVWLYIYREKIPVVPLYFAAPRPVVARDGAWIMVDDARLPLHPGETPQ
LRSVRFRTLGCYPLTGAIESSADTLEAVIAEMLVSTSSERQGRMIDHAPGASMEQKKVEGYF
>Mature_301_residues
TLPPLSHLDRLEAESIHILREVAAEFRAPVMLYSVGKDSSVLLHLLLKAFAPSRPPIPLLHIDTRWKFREMIAFRDRRAA
ETGVDLRVHINPDGVAQDVSPISHGAAVHTDIMKTQGLKQALEQGGFDAAIGGARRDEEKSRAKERVFSFRNARHRWDPK
NQRPELWNLYNARTKPGESVRVFPLSNWTELDVWLYIYREKIPVVPLYFAAPRPVVARDGAWIMVDDARLPLHPGETPQL
RSVRFRTLGCYPLTGAIESSADTLEAVIAEMLVSTSSERQGRMIDHAPGASMEQKKVEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789109, Length=297, Percent_Identity=67.3400673400673, Blast_Score=421, Evalue=1e-119,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =2.7.7.4 [H]

Molecular weight: Translated: 34133; Mature: 34001

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPPLSHLDRLEAESIHILREVAAEFRAPVMLYSVGKDSSVLLHLLLKAFAPSRPPIPL
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEE
LHIDTRWKFREMIAFRDRRAAETGVDLRVHINPDGVAQDVSPISHGAAVHTDIMKTQGLK
EEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHH
QALEQGGFDAAIGGARRDEEKSRAKERVFSFRNARHRWDPKNQRPELWNLYNARTKPGES
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCC
VRVFPLSNWTELDVWLYIYREKIPVVPLYFAAPRPVVARDGAWIMVDDARLPLHPGETPQ
EEEEECCCCCEEEEEEEEEECCCCEEEEEECCCCCEEECCCCEEEEECCCCCCCCCCCCC
LRSVRFRTLGCYPLTGAIESSADTLEAVIAEMLVSTSSERQGRMIDHAPGASMEQKKVEG
HHHEEEEEECCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHCCC
YF
CC
>Mature Secondary Structure 
TLPPLSHLDRLEAESIHILREVAAEFRAPVMLYSVGKDSSVLLHLLLKAFAPSRPPIPL
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCEE
LHIDTRWKFREMIAFRDRRAAETGVDLRVHINPDGVAQDVSPISHGAAVHTDIMKTQGLK
EEECCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHHHH
QALEQGGFDAAIGGARRDEEKSRAKERVFSFRNARHRWDPKNQRPELWNLYNARTKPGES
HHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCC
VRVFPLSNWTELDVWLYIYREKIPVVPLYFAAPRPVVARDGAWIMVDDARLPLHPGETPQ
EEEEECCCCCEEEEEEEEEECCCCEEEEEECCCCCEEECCCCEEEEECCCCCCCCCCCCC
LRSVRFRTLGCYPLTGAIESSADTLEAVIAEMLVSTSSERQGRMIDHAPGASMEQKKVEG
HHHEEEEEECCEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCHHHHCCC
YF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]