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The map label for this gene is yfiF [H]

Identifier: 84624847

GI number: 84624847

Start: 3610033

End: 3610944

Strand: Reverse

Name: yfiF [H]

Synonym: XOO_3190

Alternate gene names: 84624847

Gene position: 3610944-3610033 (Counterclockwise)

Preceding gene: 84624849

Following gene: 84624846

Centisome position: 73.09

GC content: 69.19

Gene sequence:

>912_bases
ATGAAGCAGCACGATGGGCGCGGCGCGCGTCCTCCTCGCGAGGGCGCCCCGCGAGGAAGCACCGCCGGCAATCCCTGGAA
TCGGTCGCCGCCGCGCAGCACACCGGCAGCGGCGCCATCCTCGACAACGTTGGCCTCGCCGGGCGGCAGTCCTGCCGCCG
GCAGGGCTGCTGGCCATGCACGCGCCGAAATGCGGCTGTACGGCATCAACGCCGTGCAGGCCGTGTTCAAGGCGCGCCCG
CAGGCACTGCGCAAGCTCTATCTGAGCGAGGCGCGCATTCCACAGTTCAAGGCGCTGCTGGCCTGGTGCGTGAGCCAACG
CATCGGTTATCGCGTGGTGGAAGAGGCCGATCTGAGCAAGCTCGCTGCCAGTGCGCACCATGAAGGCATCGTGGCCGAGG
TGCTGCGCGTCGCCCCGCAACCGTTGCAGGAGTGGCTGGCGGCCCTGCCGCAAGGCCCGGTGCTCGCGCTATGGCTGGAT
GGGGTGGGCAACCCGCATAACTTTGGCGCGATGTTGCGCTCGTCGGCACACTTCGGTGTTGCCGGTCTGCTGCTGCCGGC
CGGCTCCACGCTGGGCCTGTCCGGGGCGGCGGCACGCGTGGCCGAAGGTGGCGCCGAAGCGGTACCGCTGGTGCAACTGC
CCGACACCGCAACCGCGATGGCGCAATTGCGTCAGGCCGGTTTCGCGATTGCCGCCACGCTGGTCGACGGCGGCCAGGAT
GTCTTCGCTGCGGCATTGCCACAGCGCCTGGTCTACGTGATGGGCGCGGAGAGCGAAGGCATGGACCGCGCATTTGCCCG
CGACTGCGATCTGCAGTTGTCGATCCGCGGTAGCGGCAAGGTGGAAAGTCTCAACGTCGCCTCTGCCACTGCGGTCTTCC
AGGCCGCCTGGCACGCGCGCGTCCTCGCCTGA

Upstream 100 bases:

>100_bases
CTGCTTCGCCTGCGATAGCTTTGCCGGATACTCCTGCGGTCCGGCGTGTCATGCGTGTAGCAGTACCTGCGTCGCACCGC
AGCACAACCTGGACAACGTG

Downstream 100 bases:

>100_bases
GAACACCGATGTTGCGTCTTTCTGCGTTGCTGCTTGCGCGCAGTTGTGTGTTCAGCGCCAGCGCGTTTGCTGTAACGCCT
GTCGCAGCTCCCGAGGTGCG

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MKQHDGRGARPPREGAPRGSTAGNPWNRSPPRSTPAAAPSSTTLASPGGSPAAGRAAGHARAEMRLYGINAVQAVFKARP
QALRKLYLSEARIPQFKALLAWCVSQRIGYRVVEEADLSKLAASAHHEGIVAEVLRVAPQPLQEWLAALPQGPVLALWLD
GVGNPHNFGAMLRSSAHFGVAGLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDTATAMAQLRQAGFAIAATLVDGGQD
VFAAALPQRLVYVMGAESEGMDRAFARDCDLQLSIRGSGKVESLNVASATAVFQAAWHARVLA

Sequences:

>Translated_303_residues
MKQHDGRGARPPREGAPRGSTAGNPWNRSPPRSTPAAAPSSTTLASPGGSPAAGRAAGHARAEMRLYGINAVQAVFKARP
QALRKLYLSEARIPQFKALLAWCVSQRIGYRVVEEADLSKLAASAHHEGIVAEVLRVAPQPLQEWLAALPQGPVLALWLD
GVGNPHNFGAMLRSSAHFGVAGLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDTATAMAQLRQAGFAIAATLVDGGQD
VFAAALPQRLVYVMGAESEGMDRAFARDCDLQLSIRGSGKVESLNVASATAVFQAAWHARVLA
>Mature_303_residues
MKQHDGRGARPPREGAPRGSTAGNPWNRSPPRSTPAAAPSSTTLASPGGSPAAGRAAGHARAEMRLYGINAVQAVFKARP
QALRKLYLSEARIPQFKALLAWCVSQRIGYRVVEEADLSKLAASAHHEGIVAEVLRVAPQPLQEWLAALPQGPVLALWLD
GVGNPHNFGAMLRSSAHFGVAGLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDTATAMAQLRQAGFAIAATLVDGGQD
VFAAALPQRLVYVMGAESEGMDRAFARDCDLQLSIRGSGKVESLNVASATAVFQAAWHARVLA

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788935, Length=297, Percent_Identity=36.026936026936, Blast_Score=173, Evalue=1e-44,
Organism=Escherichia coli, GI1790623, Length=248, Percent_Identity=35.0806451612903, Blast_Score=119, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123
- InterPro:   IPR016479 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 31492; Mature: 31492

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS00962 RIBOSOMAL_S2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQHDGRGARPPREGAPRGSTAGNPWNRSPPRSTPAAAPSSTTLASPGGSPAAGRAAGHA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCH
RAEMRLYGINAVQAVFKARPQALRKLYLSEARIPQFKALLAWCVSQRIGYRVVEEADLSK
HHEEEEECHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
LAASAHHEGIVAEVLRVAPQPLQEWLAALPQGPVLALWLDGVGNPHNFGAMLRSSAHFGV
HHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCH
AGLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDTATAMAQLRQAGFAIAATLVDGGQD
HHHEECCCCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCHHEEEEECCCCHH
VFAAALPQRLVYVMGAESEGMDRAFARDCDLQLSIRGSGKVESLNVASATAVFQAAWHAR
HHHHHHHHHEEEEECCCCCCCHHHHHCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHH
VLA
HCC
>Mature Secondary Structure
MKQHDGRGARPPREGAPRGSTAGNPWNRSPPRSTPAAAPSSTTLASPGGSPAAGRAAGHA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCH
RAEMRLYGINAVQAVFKARPQALRKLYLSEARIPQFKALLAWCVSQRIGYRVVEEADLSK
HHEEEEECHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHH
LAASAHHEGIVAEVLRVAPQPLQEWLAALPQGPVLALWLDGVGNPHNFGAMLRSSAHFGV
HHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCH
AGLLLPAGSTLGLSGAAARVAEGGAEAVPLVQLPDTATAMAQLRQAGFAIAATLVDGGQD
HHHEECCCCCCCCCHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHCHHEEEEECCCCHH
VFAAALPQRLVYVMGAESEGMDRAFARDCDLQLSIRGSGKVESLNVASATAVFQAAWHAR
HHHHHHHHHEEEEECCCCCCCHHHHHCCCCEEEEECCCCCEEEECHHHHHHHHHHHHHHH
VLA
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]