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The map label for this gene is insK [C]

Identifier: 84624828

GI number: 84624828

Start: 3591072

End: 3591827

Strand: Reverse

Name: insK [C]

Synonym: XOO_3171

Alternate gene names: 84624828

Gene position: 3591827-3591072 (Counterclockwise)

Preceding gene: 84624829

Following gene: 84624826

Centisome position: 72.71

GC content: 64.02

Gene sequence:

>756_bases
GTGCGCGAGTGGATCGACGGTGGCGCCAGCGAGCGCTGCGCCCTGGCAGCGATCGGCATGAGCGCCAGTGCGCTGCGCTA
TCGCCCGCGCGAGGACCGCAACGTTGAGTTGCGCGAGCGCATCCTTGCGTTGGCGCATCGCCATCGCCGCTATGGCGTGG
GGATGATCTCTCTCAAGCTGCGGCAGGAAGGGCGTCTCGTGAACTATAAGCGGGTGGAGCGGCTGTATTGCGAGCAGCAG
CTGCAGGTCCGCCGCCGCACGCGTAAAAAGGTGCCGCTAGGCGAGCGTGCGCCGTTGCTGCGGCCCACCAAGGCCAACCA
GGTGTGGTCGATGGACGTCGTGTTCGACCGCACCGCCGAAGGCAGGGCAATCAAATGCCTGGTGATCGTGGACGACGCAA
CCCAAGAAGCGGTCGCCATCGACGTGGAGCGTGCGATCTCGGGACACGGCGTTGTGCGCGTGCTGGATCGGTTGGCACAC
AGTCGTGGCCTGCCGAAGATGATCCGCACGGACAACGGCAAGGAGTTCTGTGGCAAGGCCATGGTCGCCTGGGCGCATGC
CAATGGTGTGCAGCTACGCCAGATCCAGCCTGGCAAGCCGAACCAGAATGCCTACGTCGAATCCTTCAACGGCCGGCTAC
GCGACGAATGCCTCAACGAACACTGGTTCCCAACGCTGCTGCATGCGCGCACCGAGATCGAACGCTGGCGCCGCGAATAC
AACGAACACCGCCCCAAAAAACAATCGGCGGAATGA

Upstream 100 bases:

>100_bases
CGCGACTGAAGAAGTTGCTGGCCGAGCAGGTGTTCCAGAACGACCTGATCAAGGATGCGCTGCAAAAACAATGGTGAGCG
CACCGGCGCGTCGTGCACTG

Downstream 100 bases:

>100_bases
CGCCGGCGGCCTATGCCCAGCAGTTGGCCAATAGCGAGATCATCAACCCCGGACTCTAAACCCGACTGCTACTCAGGATG
GGGGGACGTCGGCGCAGCCG

Product: ISXoo3 transposase orfB

Products: NA

Alternate protein names: ORF1 [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MREWIDGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKLRQEGRLVNYKRVERLYCEQQ
LQVRRRTRKKVPLGERAPLLRPTKANQVWSMDVVFDRTAEGRAIKCLVIVDDATQEAVAIDVERAISGHGVVRVLDRLAH
SRGLPKMIRTDNGKEFCGKAMVAWAHANGVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHWFPTLLHARTEIERWRREY
NEHRPKKQSAE

Sequences:

>Translated_251_residues
MREWIDGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKLRQEGRLVNYKRVERLYCEQQ
LQVRRRTRKKVPLGERAPLLRPTKANQVWSMDVVFDRTAEGRAIKCLVIVDDATQEAVAIDVERAISGHGVVRVLDRLAH
SRGLPKMIRTDNGKEFCGKAMVAWAHANGVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHWFPTLLHARTEIERWRREY
NEHRPKKQSAE
>Mature_251_residues
MREWIDGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKLRQEGRLVNYKRVERLYCEQQ
LQVRRRTRKKVPLGERAPLLRPTKANQVWSMDVVFDRTAEGRAIKCLVIVDDATQEAVAIDVERAISGHGVVRVLDRLAH
SRGLPKMIRTDNGKEFCGKAMVAWAHANGVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHWFPTLLHARTEIERWRREY
NEHRPKKQSAE

Specific function: Involved in the transposition of the insertion sequence is150 [C]

COG id: COG2801

COG function: function code L; Transposase and inactivated derivatives

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1789981, Length=228, Percent_Identity=23.2456140350877, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 29093; Mature: 29093

Theoretical pI: Translated: 10.73; Mature: 10.73

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREWIDGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKL
CCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
RQEGRLVNYKRVERLYCEQQLQVRRRTRKKVPLGERAPLLRPTKANQVWSMDVVFDRTAE
ECCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCC
GRAIKCLVIVDDATQEAVAIDVERAISGHGVVRVLDRLAHSRGLPKMIRTDNGKEFCGKA
CCEEEEEEEECCCCCCHHEEEHHHHHCCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHH
MVAWAHANGVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHWFPTLLHARTEIERWRREY
HEEEECCCCEEEEEECCCCCCCCCHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NEHRPKKQSAE
HHCCCCCCCCC
>Mature Secondary Structure
MREWIDGGASERCALAAIGMSASALRYRPREDRNVELRERILALAHRHRRYGVGMISLKL
CCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEE
RQEGRLVNYKRVERLYCEQQLQVRRRTRKKVPLGERAPLLRPTKANQVWSMDVVFDRTAE
ECCCCEECHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEEECCCC
GRAIKCLVIVDDATQEAVAIDVERAISGHGVVRVLDRLAHSRGLPKMIRTDNGKEFCGKA
CCEEEEEEEECCCCCCHHEEEHHHHHCCCHHHHHHHHHHHHCCCCHHHHCCCCHHHHHHH
MVAWAHANGVQLRQIQPGKPNQNAYVESFNGRLRDECLNEHWFPTLLHARTEIERWRREY
HEEEECCCCEEEEEECCCCCCCCCHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
NEHRPKKQSAE
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1718819 [H]