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The map label for this gene is rpfG [H]

Identifier: 84624297

GI number: 84624297

Start: 2952088

End: 2953074

Strand: Direct

Name: rpfG [H]

Synonym: XOO_2640

Alternate gene names: 84624297

Gene position: 2952088-2953074 (Clockwise)

Preceding gene: 84624296

Following gene: 84624300

Centisome position: 59.76

GC content: 65.65

Gene sequence:

>987_bases
ATGCCGTCCCGCCCCCTGTTGTGTGTCGACGACGAATCCAGCAACCTGGCCACCCTGCGCCAGCTGCTGCGCGACGACTT
CGCGCTGGTGTTCGCCAAATCCGGCGGCGAAGCCCTGGATGCGGTGACGCGCCACACGCCCAAGCTCATCCTGCTGGATG
TCGAATTGCCGGACATGGACGGCTATGCGGTGGCGCGCGCGCTCAAGCAGCAGCCGTCCAGCAACGCCATCCCGATTCTG
TTCGTGAGTTCGCGCAACAGCGAGCACGACGAACGCCTCGGGCTGGAGGCAGGCGCGGCCGATTACGTCAGCAAGCCCTA
CTCGCCTACGCTGCTCAAGGCACGTATCGCCACTCAATTGAAGCTGGCCGAGAACGTACGCCTTGCCCAGCAATACCGCG
ACGCGATCCATTTGCTCGGCACCGCCGGCCGGGGCCAGGACGCCAACACCGGCGCACATATCTGGCGCATGGCGGTGTAT
GCGCGGGTCCTGGCCGAAGCAGTGGGCTGGTCGCCGCAGCAGGCGCAGACCCTGCAGGACGCCGCCCCGCTGCACGACGC
CGGCAAGATCGCGGTGCCCGGCAGCATTCTGCAAAAGCCCGATTCGCTGGACGAACACGAACGCTCGATGGTGCGTCAGC
ACCCGCAAGCCGGCCACGATCTCTTGCGCCACGGCCGCGGTGCGATATTCCAGCTGGCAGCGGAAATCGCGCTGCATCAT
CACGAATGCTGGGACGGCAGCGGCTATCCCGAGGGGTTGTCCGGAGATGCCATTCCCGAATCGGCACGCATCGTGGCGGT
GGCCGACGTGTTCGACGCGCTGTGCGGGCGGCGCGCCTACAAGGCGCCATGGACCGTGGAAGACGCCATGCGCAAGCTCG
ACAGCATGGCAGGCAAACAATTGGAACCGCGCCTGGTGCAGCATTTCCGTGAGGCATTACCGCAGATTCTGCAGATCAAG
GACGCCTGGGACAGCCCTGCGCCATAG

Upstream 100 bases:

>100_bases
CGAAGATTTCGACTTCCGTCGCGCCGAAACCACGTTGCGCGAGTGGCAGACGACCCACCTGCACTGATCGCGCTCCCCCG
AGCCTTGGCAAGGATCCACC

Downstream 100 bases:

>100_bases
CGTGATCTAGGGATGGTCTGATCAACGCGGCCGGGAAATGAAGCAAGCCACATCTGCGGCGCTGAGGGCGCTCAAACCCC
TGATTTTTTGCCGTTTTCGG

Product: two-component system regulatory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 327

Protein sequence:

>328_residues
MPSRPLLCVDDESSNLATLRQLLRDDFALVFAKSGGEALDAVTRHTPKLILLDVELPDMDGYAVARALKQQPSSNAIPIL
FVSSRNSEHDERLGLEAGAADYVSKPYSPTLLKARIATQLKLAENVRLAQQYRDAIHLLGTAGRGQDANTGAHIWRMAVY
ARVLAEAVGWSPQQAQTLQDAAPLHDAGKIAVPGSILQKPDSLDEHERSMVRQHPQAGHDLLRHGRGAIFQLAAEIALHH
HECWDGSGYPEGLSGDAIPESARIVAVADVFDALCGRRAYKAPWTVEDAMRKLDSMAGKQLEPRLVQHFREALPQILQIK
DAWDSPAP

Sequences:

>Translated_328_residues
MPSRPLLCVDDESSNLATLRQLLRDDFALVFAKSGGEALDAVTRHTPKLILLDVELPDMDGYAVARALKQQPSSNAIPIL
FVSSRNSEHDERLGLEAGAADYVSKPYSPTLLKARIATQLKLAENVRLAQQYRDAIHLLGTAGRGQDANTGAHIWRMAVY
ARVLAEAVGWSPQQAQTLQDAAPLHDAGKIAVPGSILQKPDSLDEHERSMVRQHPQAGHDLLRHGRGAIFQLAAEIALHH
HECWDGSGYPEGLSGDAIPESARIVAVADVFDALCGRRAYKAPWTVEDAMRKLDSMAGKQLEPRLVQHFREALPQILQIK
DAWDSPAP
>Mature_327_residues
PSRPLLCVDDESSNLATLRQLLRDDFALVFAKSGGEALDAVTRHTPKLILLDVELPDMDGYAVARALKQQPSSNAIPILF
VSSRNSEHDERLGLEAGAADYVSKPYSPTLLKARIATQLKLAENVRLAQQYRDAIHLLGTAGRGQDANTGAHIWRMAVYA
RVLAEAVGWSPQQAQTLQDAAPLHDAGKIAVPGSILQKPDSLDEHERSMVRQHPQAGHDLLRHGRGAIFQLAAEIALHHH
ECWDGSGYPEGLSGDAIPESARIVAVADVFDALCGRRAYKAPWTVEDAMRKLDSMAGKQLEPRLVQHFREALPQILQIKD
AWDSPAP

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790860, Length=114, Percent_Identity=39.4736842105263, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI1788394, Length=190, Percent_Identity=32.1052631578947, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1789809, Length=121, Percent_Identity=37.1900826446281, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1790863, Length=247, Percent_Identity=25.9109311740891, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1786599, Length=124, Percent_Identity=33.8709677419355, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1786911, Length=118, Percent_Identity=38.9830508474576, Blast_Score=68, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 35905; Mature: 35774

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSRPLLCVDDESSNLATLRQLLRDDFALVFAKSGGEALDAVTRHTPKLILLDVELPDMD
CCCCCEEEECCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCEEEEEEEECCCCC
GYAVARALKQQPSSNAIPILFVSSRNSEHDERLGLEAGAADYVSKPYSPTLLKARIATQL
HHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHH
KLAENVRLAQQYRDAIHLLGTAGRGQDANTGAHIWRMAVYARVLAEAVGWSPQQAQTLQD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AAPLHDAGKIAVPGSILQKPDSLDEHERSMVRQHPQAGHDLLRHGRGAIFQLAAEIALHH
HCCCCCCCCEECCHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHH
HECWDGSGYPEGLSGDAIPESARIVAVADVFDALCGRRAYKAPWTVEDAMRKLDSMAGKQ
HHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
LEPRLVQHFREALPQILQIKDAWDSPAP
CCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PSRPLLCVDDESSNLATLRQLLRDDFALVFAKSGGEALDAVTRHTPKLILLDVELPDMD
CCCCEEEECCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCEEEEEEEECCCCC
GYAVARALKQQPSSNAIPILFVSSRNSEHDERLGLEAGAADYVSKPYSPTLLKARIATQL
HHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHCCCCCCHHHHCCCCCCHHHHHHHHHHH
KLAENVRLAQQYRDAIHLLGTAGRGQDANTGAHIWRMAVYARVLAEAVGWSPQQAQTLQD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
AAPLHDAGKIAVPGSILQKPDSLDEHERSMVRQHPQAGHDLLRHGRGAIFQLAAEIALHH
HCCCCCCCCEECCHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHH
HECWDGSGYPEGLSGDAIPESARIVAVADVFDALCGRRAYKAPWTVEDAMRKLDSMAGKQ
HHCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
LEPRLVQHFREALPQILQIKDAWDSPAP
CCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]