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The map label for this gene is pleD [H]

Identifier: 84623863

GI number: 84623863

Start: 2445331

End: 2446446

Strand: Direct

Name: pleD [H]

Synonym: XOO_2206

Alternate gene names: 84623863

Gene position: 2445331-2446446 (Clockwise)

Preceding gene: 84623862

Following gene: 84623865

Centisome position: 49.5

GC content: 64.43

Gene sequence:

>1116_bases
GTGATGCTGCTGGCCTACCGTTTGTTGTCGCGCGAGCTGGAACTGCGCACGCGTGCAGAAGACGAAGCCAGCCATACCAG
CGAGCGCCTGACCGAGTCGTTTGCGGCGCTTGAACGTACCTCTGCCGGTCTGGAGGCGCTGTCGCGGTACTCGGAATTGC
TGCAGAACTGCCGCGATGCCGAAGAAGCGCTGGAAATCACTGCGCACACGATCGCCCCGCTGATGCCCGGTGCGGCCGGC
GCGGTGTACCTGCTGCGCGCCTCGCGCAACCGCGCCGAACAAGTTATCGCCTGGGGCACGATGGCCGACGACGCCGGTGC
CAACATCGCACCGGACAACTGTTTGGCGCTGCGTCGCGACCGCACCCATGTGGTGATGGACGCCAGCCAGGGCATGCTGT
ATCAGCATGCCGGCGTCAATCTCCCGCCGCAGGCGGGCACGGCCTGCATTCCGCTATCGGCGCAAGGCACGCAATTGGGA
ATGCTGGCGTTGCGCAGCGAAGACCCCAGCGATCTCGCATCACTGACGGTCGCCGAGGCGGCAGCGGAACAACTGTCGTT
GGCGCTGCACAACCTGCGCTTGCGCGAAACGCTGCGTCAGCAATCGATCCGCGACCTGCTGACCGGCCTGTACAACCGCC
GCTATCTGGAAGAAACCCTCAATCACGAACTGGCGCGCTGTACACGTCGGGGGCTGCCACTGTCGGTGCTGATGCTGGAT
GTGGATCACTTCAAGCAGTTCAACGACCTGAATGGGCACAGCGGCGGCGACCGCGTACTGTCGGCCATCGGCGAGTTGCT
GCTGTCGCAGACACGTGGTGAGGACATCTGCTGTCGGTATGGCGGAGAAGAATTGACGATCATCCTGCCCGAGGTGGATC
TGCCCACCGCCTGCATGATGGCCGAGAAGCTGCGCACCGGTATCGAGGCCTTGCAGGTGATGGGCAATGGCGTCTCCCTG
CCCAAGGTCACTGCATCGTTCGGCGCGGCCAACTTCCCCGATCACGCGGGCACCGTGGCGCAGTTGCTGCGGCGGGCGCA
CGAGGCGCTGTATCGCGCCAAACAAGTGGGCCGCAATCAGGTCGTGAGTGCGCACGTGCTAGCAACTGCGGCCTGA

Upstream 100 bases:

>100_bases
GCTGGAGCGTCAGCAGTTGGTGCGGCGAAACGACGCCACCACGCGCAGTGCGCAATGGGTGCTGTGGGTGACGGTGATCG
GTATTTTCAGCTGACTGCTG

Downstream 100 bases:

>100_bases
GCGCAGGCGGCCCAAGCGTACTTCGCCAAGCGCGTGTAGAGGCATTGGTCACGTGGCCGATTCTGGCGCTCGCATCAAGC
GCCTGCGCTTTGCGGCGATT

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MMLLAYRLLSRELELRTRAEDEASHTSERLTESFAALERTSAGLEALSRYSELLQNCRDAEEALEITAHTIAPLMPGAAG
AVYLLRASRNRAEQVIAWGTMADDAGANIAPDNCLALRRDRTHVVMDASQGMLYQHAGVNLPPQAGTACIPLSAQGTQLG
MLALRSEDPSDLASLTVAEAAAEQLSLALHNLRLRETLRQQSIRDLLTGLYNRRYLEETLNHELARCTRRGLPLSVLMLD
VDHFKQFNDLNGHSGGDRVLSAIGELLLSQTRGEDICCRYGGEELTIILPEVDLPTACMMAEKLRTGIEALQVMGNGVSL
PKVTASFGAANFPDHAGTVAQLLRRAHEALYRAKQVGRNQVVSAHVLATAA

Sequences:

>Translated_371_residues
MMLLAYRLLSRELELRTRAEDEASHTSERLTESFAALERTSAGLEALSRYSELLQNCRDAEEALEITAHTIAPLMPGAAG
AVYLLRASRNRAEQVIAWGTMADDAGANIAPDNCLALRRDRTHVVMDASQGMLYQHAGVNLPPQAGTACIPLSAQGTQLG
MLALRSEDPSDLASLTVAEAAAEQLSLALHNLRLRETLRQQSIRDLLTGLYNRRYLEETLNHELARCTRRGLPLSVLMLD
VDHFKQFNDLNGHSGGDRVLSAIGELLLSQTRGEDICCRYGGEELTIILPEVDLPTACMMAEKLRTGIEALQVMGNGVSL
PKVTASFGAANFPDHAGTVAQLLRRAHEALYRAKQVGRNQVVSAHVLATAA
>Mature_371_residues
MMLLAYRLLSRELELRTRAEDEASHTSERLTESFAALERTSAGLEALSRYSELLQNCRDAEEALEITAHTIAPLMPGAAG
AVYLLRASRNRAEQVIAWGTMADDAGANIAPDNCLALRRDRTHVVMDASQGMLYQHAGVNLPPQAGTACIPLSAQGTQLG
MLALRSEDPSDLASLTVAEAAAEQLSLALHNLRLRETLRQQSIRDLLTGLYNRRYLEETLNHELARCTRRGLPLSVLMLD
VDHFKQFNDLNGHSGGDRVLSAIGELLLSQTRGEDICCRYGGEELTIILPEVDLPTACMMAEKLRTGIEALQVMGNGVSL
PKVTASFGAANFPDHAGTVAQLLRRAHEALYRAKQVGRNQVVSAHVLATAA

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=169, Percent_Identity=38.4615384615385, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=38.5057471264368, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=35.7575757575758, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=36.6279069767442, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1786584, Length=168, Percent_Identity=33.3333333333333, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787802, Length=195, Percent_Identity=30.7692307692308, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788085, Length=166, Percent_Identity=29.5180722891566, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787816, Length=164, Percent_Identity=35.9756097560976, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1788381, Length=201, Percent_Identity=28.8557213930348, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI87081974, Length=120, Percent_Identity=30, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=28.6549707602339, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 40372; Mature: 40372

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLLAYRLLSRELELRTRAEDEASHTSERLTESFAALERTSAGLEALSRYSELLQNCRDA
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEALEITAHTIAPLMPGAAGAVYLLRASRNRAEQVIAWGTMADDAGANIAPDNCLALRRD
HHHHHHHHHHHHHHCCCCCCHHEEEHHHCCHHHHHEEECCCCCCCCCCCCCCHHHHEECC
RTHVVMDASQGMLYQHAGVNLPPQAGTACIPLSAQGTQLGMLALRSEDPSDLASLTVAEA
CCEEEEECCCCEEEECCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHH
AAEQLSLALHNLRLRETLRQQSIRDLLTGLYNRRYLEETLNHELARCTRRGLPLSVLMLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEE
VDHFKQFNDLNGHSGGDRVLSAIGELLLSQTRGEDICCRYGGEELTIILPEVDLPTACMM
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCHHHHH
AEKLRTGIEALQVMGNGVSLPKVTASFGAANFPDHAGTVAQLLRRAHEALYRAKQVGRNQ
HHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHH
VVSAHVLATAA
HHHHHHHEECC
>Mature Secondary Structure
MMLLAYRLLSRELELRTRAEDEASHTSERLTESFAALERTSAGLEALSRYSELLQNCRDA
CHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEALEITAHTIAPLMPGAAGAVYLLRASRNRAEQVIAWGTMADDAGANIAPDNCLALRRD
HHHHHHHHHHHHHHCCCCCCHHEEEHHHCCHHHHHEEECCCCCCCCCCCCCCHHHHEECC
RTHVVMDASQGMLYQHAGVNLPPQAGTACIPLSAQGTQLGMLALRSEDPSDLASLTVAEA
CCEEEEECCCCEEEECCCCCCCCCCCCEEEECCCCCCCEEEEEECCCCHHHHHHHHHHHH
AAEQLSLALHNLRLRETLRQQSIRDLLTGLYNRRYLEETLNHELARCTRRGLPLSVLMLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEE
VDHFKQFNDLNGHSGGDRVLSAIGELLLSQTRGEDICCRYGGEELTIILPEVDLPTACMM
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCEEEEEECCCCCCHHHHH
AEKLRTGIEALQVMGNGVSLPKVTASFGAANFPDHAGTVAQLLRRAHEALYRAKQVGRNQ
HHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHH
VVSAHVLATAA
HHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA