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The map label for this gene is insF1 [C]

Identifier: 84623818

GI number: 84623818

Start: 2393045

End: 2393716

Strand: Direct

Name: insF1 [C]

Synonym: XOO_2161

Alternate gene names: 84623818

Gene position: 2393045-2393716 (Clockwise)

Preceding gene: 84623817

Following gene: 84623821

Centisome position: 48.44

GC content: 66.67

Gene sequence:

>672_bases
GTGATCCAGCAGATGATCCAGCAGTGGCAGGAGAAGGCCAGAACCGCCCGGCTGTGCCGGTTGCTGGGGGTGAGTCGTTC
GGGGGTGTATGCGGCGCGCCGACGGCGACCGGCTCCACGCGCTGATGTACTTGCGGCAGCGGTGCAGACGGCCTTCCAGG
CCAGCGGCGGCAACTATGGCAGCCGTCGATTGAGCGCCAGCCTGAAGGCCCAGGGCCTGCCCGCTGGTCGCCACCGGGTC
CGCCGCCTGATAAAGCGCCACGGGCTGAAGGCGCGCTGGAAACGCAAGTTCACCCACACCACCGACAGCCGGCACGACTT
GCCTGTGGCGGCCAACGTTCTGGATCGGCGCTTCAAGCCCAGTGCTGCGGACCAAGCCTGGGTGGCCGACATCACCTACA
TCCGCACCGAGCGCGGCTGGCTGTATCTGGCCGCCGTGCTGGACCTGTACTCGCGCAAGATCGTTGGCTGGGCGATGGCA
CCGAGCATGCCTGCCGAGCTGGTGTGCTGCGCCTTGCAGATGGCCATCGTCCTGCGCCAGCCCAAGCCGGGACTGATCAT
GCATACCGACCGCGGTAGCCAATACGCCAGTCAGGCCCACCGTGATCTGCTGGCGGCGGCTTCAACTCTGGATCGCAACA
CCAACGGTTGTGACGTGGTCCAGGCGACCTGA

Upstream 100 bases:

>100_bases
AGCAGCAACGCATCCGTGAATTGGAGCGGGAAAACCAACGGCTGCGTGAGGACAACAGCCTGTTAAAAAAAGCGTCGGCC
TTCTTCGCCCGGGAACTGAA

Downstream 100 bases:

>100_bases
CCTGAGCGACTTCACCGCCAAGTGGAGTTGCCCATGTCATCCAGCCGCCTTGACCTATCGGAACGATACCGTCTGCATGC
GTTATATGAAACCGGGATGT

Product: transposase

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MIQQMIQQWQEKARTARLCRLLGVSRSGVYAARRRRPAPRADVLAAAVQTAFQASGGNYGSRRLSASLKAQGLPAGRHRV
RRLIKRHGLKARWKRKFTHTTDSRHDLPVAANVLDRRFKPSAADQAWVADITYIRTERGWLYLAAVLDLYSRKIVGWAMA
PSMPAELVCCALQMAIVLRQPKPGLIMHTDRGSQYASQAHRDLLAAASTLDRNTNGCDVVQAT

Sequences:

>Translated_223_residues
MIQQMIQQWQEKARTARLCRLLGVSRSGVYAARRRRPAPRADVLAAAVQTAFQASGGNYGSRRLSASLKAQGLPAGRHRV
RRLIKRHGLKARWKRKFTHTTDSRHDLPVAANVLDRRFKPSAADQAWVADITYIRTERGWLYLAAVLDLYSRKIVGWAMA
PSMPAELVCCALQMAIVLRQPKPGLIMHTDRGSQYASQAHRDLLAAASTLDRNTNGCDVVQAT
>Mature_223_residues
MIQQMIQQWQEKARTARLCRLLGVSRSGVYAARRRRPAPRADVLAAAVQTAFQASGGNYGSRRLSASLKAQGLPAGRHRV
RRLIKRHGLKARWKRKFTHTTDSRHDLPVAANVLDRRFKPSAADQAWVADITYIRTERGWLYLAAVLDLYSRKIVGWAMA
PSMPAELVCCALQMAIVLRQPKPGLIMHTDRGSQYASQAHRDLLAAASTLDRNTNGCDVVQAT

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=216, Percent_Identity=31.9444444444444, Blast_Score=117, Evalue=7e-27,
Organism=Escherichia coli, GI1788405, Length=198, Percent_Identity=42.9292929292929, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1787263, Length=198, Percent_Identity=42.9292929292929, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1786753, Length=198, Percent_Identity=42.9292929292929, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1786570, Length=198, Percent_Identity=42.9292929292929, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1786490, Length=198, Percent_Identity=42.9292929292929, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1789981, Length=208, Percent_Identity=26.4423076923077, Blast_Score=73, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 24882; Mature: 24882

Theoretical pI: Translated: 11.73; Mature: 11.73

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQQMIQQWQEKARTARLCRLLGVSRSGVYAARRRRPAPRADVLAAAVQTAFQASGGNYG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
SRRLSASLKAQGLPAGRHRVRRLIKRHGLKARWKRKFTHTTDSRHDLPVAANVLDRRFKP
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
SAADQAWVADITYIRTERGWLYLAAVLDLYSRKIVGWAMAPSMPAELVCCALQMAIVLRQ
CCCCCHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
PKPGLIMHTDRGSQYASQAHRDLLAAASTLDRNTNGCDVVQAT
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC
>Mature Secondary Structure
MIQQMIQQWQEKARTARLCRLLGVSRSGVYAARRRRPAPRADVLAAAVQTAFQASGGNYG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
SRRLSASLKAQGLPAGRHRVRRLIKRHGLKARWKRKFTHTTDSRHDLPVAANVLDRRFKP
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
SAADQAWVADITYIRTERGWLYLAAVLDLYSRKIVGWAMAPSMPAELVCCALQMAIVLRQ
CCCCCHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
PKPGLIMHTDRGSQYASQAHRDLLAAASTLDRNTNGCDVVQAT
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]