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The map label for this gene is insF1 [C]

Identifier: 84623656

GI number: 84623656

Start: 2210008

End: 2210856

Strand: Direct

Name: insF1 [C]

Synonym: XOO_1999

Alternate gene names: 84623656

Gene position: 2210008-2210856 (Clockwise)

Preceding gene: 84623652

Following gene: 84623660

Centisome position: 44.74

GC content: 66.31

Gene sequence:

>849_bases
GTGATCCAGCAGATGATCCAGCAGTGGCAGGAGAAGGCCAGAACCGCCCGGCTGTGCCGGTTGCTGGGGGTGAGTCGTTC
GGGGGTGTATGCGGCGCGCCGACGGCGACCGGCTCCACGCGCTGATGTACTTGCGGCAGCGGTGCAGACGGCCTTCCAGG
CCAGCGGCGGCAACTATGGCAGCCGTCGATTGAGCGCCAGCCTGAAGGCCCAGGGCCTGCCCGCTGGTCGCCACCGGGTC
CGCCGCCTGATGAAGCGCCACGGGCTGAAGGCGCGCTGGAAACGCAAGTTCACCCACACCACCGACAGCCGGCACGACTT
GCCTGTGGCGGCCAACGTTCTGGATCGGCGCTTCAAGCCCAGTGCTGCGGACCAAGCCTGGGTGGCCGACATCACCTACA
TCCGCACCGAGCGCGGCTGGCTGTATCTGGCCGCCGTGCTGGACCTGTACTCGCGCAAGATCGTTGGCTGGGCGATGGCA
CCGAGCATGCCTGCCGAGCTGGTGTGCTGCGCCTTGCAGATGGCCATCGTCCTGCGCCAGCCCAAGCCGGGACTGATCAT
GCATACCGACCGCGGTAGCCAATACGCCAGTCAGGCCCACCGTGATCTGCTGGCCAAGCACAAGCTGGTGGCCAGCATGA
GCCGCAAGGGCAACTGCTGGGACAACGCGGTCATGGAGCGGTTCTTCCTGAACCTGAAAATGGAGCGCGTCTGGCAGCGC
CGCTACGCCAACCCTGCCGAAGCCAGCGCCGACATCACCCACTACATCGTCGCCTTCTACAACACCCGACGGCTGCACTC
CACCCTCGGCTATCGATCACCGGCCGACTACGAGAACGCCGCCGCCTGA

Upstream 100 bases:

>100_bases
AGCAGCAACGCATCCGTGAATTGGAGCGGGAAAACCAACGGCTGCGTGAGGACAACAGCCTGTTAAAAAAAGCGTCGGCC
TTCTTCGCCCGGGAACTGAA

Downstream 100 bases:

>100_bases
AATCCCCTATCGCAGTGTCTACTAAAACTTGACCACGACACATTTCACGCCTGCGCCGGAGTAGAGGGTCGGCAGGGATT
CGTGTAAAACACAGCCAAAA

Product: transposase

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MIQQMIQQWQEKARTARLCRLLGVSRSGVYAARRRRPAPRADVLAAAVQTAFQASGGNYGSRRLSASLKAQGLPAGRHRV
RRLMKRHGLKARWKRKFTHTTDSRHDLPVAANVLDRRFKPSAADQAWVADITYIRTERGWLYLAAVLDLYSRKIVGWAMA
PSMPAELVCCALQMAIVLRQPKPGLIMHTDRGSQYASQAHRDLLAKHKLVASMSRKGNCWDNAVMERFFLNLKMERVWQR
RYANPAEASADITHYIVAFYNTRRLHSTLGYRSPADYENAAA

Sequences:

>Translated_282_residues
MIQQMIQQWQEKARTARLCRLLGVSRSGVYAARRRRPAPRADVLAAAVQTAFQASGGNYGSRRLSASLKAQGLPAGRHRV
RRLMKRHGLKARWKRKFTHTTDSRHDLPVAANVLDRRFKPSAADQAWVADITYIRTERGWLYLAAVLDLYSRKIVGWAMA
PSMPAELVCCALQMAIVLRQPKPGLIMHTDRGSQYASQAHRDLLAKHKLVASMSRKGNCWDNAVMERFFLNLKMERVWQR
RYANPAEASADITHYIVAFYNTRRLHSTLGYRSPADYENAAA
>Mature_282_residues
MIQQMIQQWQEKARTARLCRLLGVSRSGVYAARRRRPAPRADVLAAAVQTAFQASGGNYGSRRLSASLKAQGLPAGRHRV
RRLMKRHGLKARWKRKFTHTTDSRHDLPVAANVLDRRFKPSAADQAWVADITYIRTERGWLYLAAVLDLYSRKIVGWAMA
PSMPAELVCCALQMAIVLRQPKPGLIMHTDRGSQYASQAHRDLLAKHKLVASMSRKGNCWDNAVMERFFLNLKMERVWQR
RYANPAEASADITHYIVAFYNTRRLHSTLGYRSPADYENAAA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=217, Percent_Identity=33.6405529953917, Blast_Score=128, Evalue=6e-30,
Organism=Escherichia coli, GI1788405, Length=280, Percent_Identity=41.4285714285714, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1787263, Length=280, Percent_Identity=41.4285714285714, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1786753, Length=280, Percent_Identity=41.4285714285714, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1786570, Length=280, Percent_Identity=41.4285714285714, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1786490, Length=280, Percent_Identity=41.4285714285714, Blast_Score=209, Evalue=1e-55,
Organism=Escherichia coli, GI1789981, Length=266, Percent_Identity=31.9548872180451, Blast_Score=132, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32075; Mature: 32075

Theoretical pI: Translated: 11.45; Mature: 11.45

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQQMIQQWQEKARTARLCRLLGVSRSGVYAARRRRPAPRADVLAAAVQTAFQASGGNYG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
SRRLSASLKAQGLPAGRHRVRRLMKRHGLKARWKRKFTHTTDSRHDLPVAANVLDRRFKP
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
SAADQAWVADITYIRTERGWLYLAAVLDLYSRKIVGWAMAPSMPAELVCCALQMAIVLRQ
CCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
PKPGLIMHTDRGSQYASQAHRDLLAKHKLVASMSRKGNCWDNAVMERFFLNLKMERVWQR
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RYANPAEASADITHYIVAFYNTRRLHSTLGYRSPADYENAAA
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MIQQMIQQWQEKARTARLCRLLGVSRSGVYAARRRRPAPRADVLAAAVQTAFQASGGNYG
CHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCC
SRRLSASLKAQGLPAGRHRVRRLMKRHGLKARWKRKFTHTTDSRHDLPVAANVLDRRFKP
HHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
SAADQAWVADITYIRTERGWLYLAAVLDLYSRKIVGWAMAPSMPAELVCCALQMAIVLRQ
CCCCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
PKPGLIMHTDRGSQYASQAHRDLLAKHKLVASMSRKGNCWDNAVMERFFLNLKMERVWQR
CCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
RYANPAEASADITHYIVAFYNTRRLHSTLGYRSPADYENAAA
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]