Click here to switch to the map view.

The map label for this gene is lepA [H]

Identifier: 84623405

GI number: 84623405

Start: 1932418

End: 1934223

Strand: Direct

Name: lepA [H]

Synonym: XOO_1748

Alternate gene names: 84623405

Gene position: 1932418-1934223 (Clockwise)

Preceding gene: 84623404

Following gene: 84623406

Centisome position: 39.12

GC content: 62.18

Gene sequence:

>1806_bases
ATGTCCTCTGATTCAATGCGGAATATCCGCAATTTTTCCATCATTGCCCACGTCGACCACGGGAAATCCACCCTTGCCGA
CCGGATCATCCAGCTGTGCGGCGGCCTGCAGGCGCGCGAGATGGAGGCCCAGGTGCTCGACTCCAACCCGATCGAGCGCG
AACGCGGCATCACCATCAAGGCGCAGTCGGTGTCCTTGCCTTACACGGCAAAGGATGGGCAGGTCTACCATCTGAACTTC
ATCGACACCCCCGGGCACGTCGACTTCTCCTACGAAGTCAGCCGCTCGCTGGCCGCCTGCGAGGGCGCGCTGCTGGTGGT
GGATGCGGCGCAGGGCGTGGAAGCGCAGTCGGTGGCCAACTGCTACACGGCAGTGGAGCAGGGCCTGGAAGTGGTGCCGG
TGCTCAACAAGATCGACCTGCCCACCGCCGATGTCGACCGCGCCAAGGCCGAGATCGAAGCGGTGATCGGCATCGATGCC
GAAGATGCGGTGGCGGTGAGCGCCAAGACCGGGTTGAACATCGATCTGGTGCTGGAGGCGATCGTGCATCGCATCCCGCC
GCCGGCCCCGCGCGACACCGACAAGCTGCAGGCGCTGATCATCGATTCCTGGTTCGACAACTACCTGGGCGTGGTCTCGC
TGGTACGCGTGATGCAAGGCGAGATCAAGCCGGGCAGCAAAATCCTGGTGATGTCCACCGGCCGCACGCATCTGGTCGAC
AAGGTCGGCGTGTTCACGCCCAAGCGCAAGGAGCTGTCTGCGCTGGGCGCTGGCGAGGTGGGCTGGATCAACGCCTCGAT
CAAGGACGTGCACGGCGCACCGGTCGGCGACACGCTGACCCTGGCGGCCGACCCGGCGCCGCACGCATTGCCCGGTTTCC
AGGAAATGCAGCCGCGTGTGTTCGCCGGCCTGTTTCCGGTCGATGCCGAGGATTACCCGGACCTGCGCGAAGCGCTGGAC
AAGCTGCGTTTGAACGATGCCGCACTGCGCTTCGAGCCGGAAAGCTCCGAAGCGATGGGTTTCGGCTTCCGCTGCGGCTT
CCTCGGCATGCTGCACATGGAAATCGTGCAGGAACGCCTGGAGCGCGAGTACAACCTCAACCTGATCTCGACCGCGCCCA
CGGTGGTTTACGAGGTGCTCAAGACCGACGGCAGCGTGATCCCGATGGACAACCCGTCCAAGCTGCCGCCGCTCAACAAC
GTCGAAGAAATCCGCGAACCGATCATTCGCGCCAATATCCTCACGCCGCCCGATTACGTCGGCAACATCATCACCTTGTG
CGAAGAAAAGCGCGGCAGCCAGATCGGCATCAACTACCTGGGCAGTCAGGTGCAGATTAGCTACGAATTGCCGATGGCCG
AAGTAGTGCTGGATTTCTTCGACAAGCTCAAGTCGGTCTCGCGCGGTTACGCCTCGCTGGACTATCACTTCCTGCGTTTC
GACCCGGGCCCGTTCGTGCGCGTGGACACGCTGATCAACGGCGACAAGGTCGATGCGCTGAGCATCATCGTGCACCGCAG
CTACGCCGACCGCCGTGGCCGCGAGTTGTGCGAAAAGATGAAAGACCTGATTCCACGGCAGATGTTCGACGTGGCGATCC
AGGCCGCGGTCGGCTCGCAAATCATCTCGCGCTCCACGGTCAAGGCGATGCGCAAGAACGTGTTGGCCAAGTGCTATGGT
GGCGACGTTTCGCGCAAGAAAAAGCTGCTGGAAAAGCAGAAAGAAGGCAAGAAGCGCATGAAGCAGGTCGGTCGCGTGGA
GATTCCGCAGGAAGCCTTCCTGGCTGTCTTGCAGATGGACAAGTAG

Upstream 100 bases:

>100_bases
CGCTGCCGTTTCGAATCCCCAATCCCGACTCCCTGACCCCTGCGTCAGCATGCGATAATGCTGCGTTACCCTGACGACGG
CACCGCCGGCGCCCACCTCA

Downstream 100 bases:

>100_bases
CAGGGATTCGGGATTCGTCATTCGTGATTGGCAACGGCTTGCCCATCCTCTTGCTCTTAACTAATCCCGAATCTCAAATT
TCGAATCCCGTTCCGAAGGA

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 601; Mature: 600

Protein sequence:

>601_residues
MSSDSMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLQAREMEAQVLDSNPIERERGITIKAQSVSLPYTAKDGQVYHLNF
IDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCYTAVEQGLEVVPVLNKIDLPTADVDRAKAEIEAVIGIDA
EDAVAVSAKTGLNIDLVLEAIVHRIPPPAPRDTDKLQALIIDSWFDNYLGVVSLVRVMQGEIKPGSKILVMSTGRTHLVD
KVGVFTPKRKELSALGAGEVGWINASIKDVHGAPVGDTLTLAADPAPHALPGFQEMQPRVFAGLFPVDAEDYPDLREALD
KLRLNDAALRFEPESSEAMGFGFRCGFLGMLHMEIVQERLEREYNLNLISTAPTVVYEVLKTDGSVIPMDNPSKLPPLNN
VEEIREPIIRANILTPPDYVGNIITLCEEKRGSQIGINYLGSQVQISYELPMAEVVLDFFDKLKSVSRGYASLDYHFLRF
DPGPFVRVDTLINGDKVDALSIIVHRSYADRRGRELCEKMKDLIPRQMFDVAIQAAVGSQIISRSTVKAMRKNVLAKCYG
GDVSRKKKLLEKQKEGKKRMKQVGRVEIPQEAFLAVLQMDK

Sequences:

>Translated_601_residues
MSSDSMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLQAREMEAQVLDSNPIERERGITIKAQSVSLPYTAKDGQVYHLNF
IDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCYTAVEQGLEVVPVLNKIDLPTADVDRAKAEIEAVIGIDA
EDAVAVSAKTGLNIDLVLEAIVHRIPPPAPRDTDKLQALIIDSWFDNYLGVVSLVRVMQGEIKPGSKILVMSTGRTHLVD
KVGVFTPKRKELSALGAGEVGWINASIKDVHGAPVGDTLTLAADPAPHALPGFQEMQPRVFAGLFPVDAEDYPDLREALD
KLRLNDAALRFEPESSEAMGFGFRCGFLGMLHMEIVQERLEREYNLNLISTAPTVVYEVLKTDGSVIPMDNPSKLPPLNN
VEEIREPIIRANILTPPDYVGNIITLCEEKRGSQIGINYLGSQVQISYELPMAEVVLDFFDKLKSVSRGYASLDYHFLRF
DPGPFVRVDTLINGDKVDALSIIVHRSYADRRGRELCEKMKDLIPRQMFDVAIQAAVGSQIISRSTVKAMRKNVLAKCYG
GDVSRKKKLLEKQKEGKKRMKQVGRVEIPQEAFLAVLQMDK
>Mature_600_residues
SSDSMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLQAREMEAQVLDSNPIERERGITIKAQSVSLPYTAKDGQVYHLNFI
DTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVANCYTAVEQGLEVVPVLNKIDLPTADVDRAKAEIEAVIGIDAE
DAVAVSAKTGLNIDLVLEAIVHRIPPPAPRDTDKLQALIIDSWFDNYLGVVSLVRVMQGEIKPGSKILVMSTGRTHLVDK
VGVFTPKRKELSALGAGEVGWINASIKDVHGAPVGDTLTLAADPAPHALPGFQEMQPRVFAGLFPVDAEDYPDLREALDK
LRLNDAALRFEPESSEAMGFGFRCGFLGMLHMEIVQERLEREYNLNLISTAPTVVYEVLKTDGSVIPMDNPSKLPPLNNV
EEIREPIIRANILTPPDYVGNIITLCEEKRGSQIGINYLGSQVQISYELPMAEVVLDFFDKLKSVSRGYASLDYHFLRFD
PGPFVRVDTLINGDKVDALSIIVHRSYADRRGRELCEKMKDLIPRQMFDVAIQAAVGSQIISRSTVKAMRKNVLAKCYGG
DVSRKKKLLEKQKEGKKRMKQVGRVEIPQEAFLAVLQMDK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=607, Percent_Identity=48.1054365733114, Blast_Score=602, Evalue=1e-172,
Organism=Homo sapiens, GI94966754, Length=133, Percent_Identity=45.8646616541353, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI4503483, Length=148, Percent_Identity=42.5675675675676, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI25306283, Length=134, Percent_Identity=39.5522388059701, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI25306287, Length=134, Percent_Identity=41.7910447761194, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI19923640, Length=134, Percent_Identity=41.7910447761194, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI310132016, Length=110, Percent_Identity=44.5454545454545, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI310110807, Length=110, Percent_Identity=44.5454545454545, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI310123363, Length=110, Percent_Identity=44.5454545454545, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI18390331, Length=158, Percent_Identity=34.8101265822785, Blast_Score=90, Evalue=7e-18,
Organism=Homo sapiens, GI217272892, Length=139, Percent_Identity=35.9712230215827, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI217272894, Length=139, Percent_Identity=35.9712230215827, Blast_Score=81, Evalue=3e-15,
Organism=Homo sapiens, GI53729339, Length=227, Percent_Identity=29.5154185022026, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI53729337, Length=227, Percent_Identity=29.5154185022026, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI34147630, Length=131, Percent_Identity=36.6412213740458, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=69.2953020134228, Blast_Score=844, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=510, Percent_Identity=28.0392156862745, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789738, Length=155, Percent_Identity=34.1935483870968, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1790835, Length=137, Percent_Identity=33.5766423357664, Blast_Score=76, Evalue=8e-15,
Organism=Escherichia coli, GI1789559, Length=231, Percent_Identity=31.1688311688312, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1789737, Length=134, Percent_Identity=33.5820895522388, Blast_Score=63, Evalue=4e-11,
Organism=Escherichia coli, GI1790412, Length=134, Percent_Identity=33.5820895522388, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=40.1960784313725, Blast_Score=460, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17506493, Length=160, Percent_Identity=37.5, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=463, Percent_Identity=24.4060475161987, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=36.8421052631579, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=36.8421052631579, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=145, Percent_Identity=35.1724137931034, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17533571, Length=137, Percent_Identity=34.3065693430657, Blast_Score=82, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=143, Percent_Identity=37.0629370629371, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI32566303, Length=306, Percent_Identity=29.0849673202614, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323320, Length=602, Percent_Identity=46.5116279069767, Blast_Score=538, Evalue=1e-154,
Organism=Saccharomyces cerevisiae, GI6324707, Length=188, Percent_Identity=38.2978723404255, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320593, Length=188, Percent_Identity=38.2978723404255, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6323098, Length=158, Percent_Identity=36.0759493670886, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6322359, Length=115, Percent_Identity=36.5217391304348, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=144, Percent_Identity=40.2777777777778, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6324761, Length=271, Percent_Identity=29.8892988929889, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI78706572, Length=600, Percent_Identity=45, Blast_Score=525, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24585711, Length=191, Percent_Identity=34.0314136125654, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585713, Length=191, Percent_Identity=34.0314136125654, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24585709, Length=191, Percent_Identity=34.0314136125654, Blast_Score=104, Evalue=2e-22,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=43.8848920863309, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24582462, Length=184, Percent_Identity=32.0652173913043, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI221458488, Length=148, Percent_Identity=36.4864864864865, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI21357743, Length=134, Percent_Identity=36.5671641791045, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28572034, Length=262, Percent_Identity=27.4809160305344, Blast_Score=77, Evalue=4e-14,
Organism=Drosophila melanogaster, GI281363316, Length=149, Percent_Identity=31.5436241610738, Blast_Score=66, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17864358, Length=149, Percent_Identity=31.5436241610738, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 66227; Mature: 66096

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDSMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLQAREMEAQVLDSNPIERERGITIK
CCCCHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHCCCEEE
AQSVSLPYTAKDGQVYHLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVAN
EEEEECCEECCCCEEEEEEEECCCCCCEEEHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
CYTAVEQGLEVVPVLNKIDLPTADVDRAKAEIEAVIGIDAEDAVAVSAKTGLNIDLVLEA
HHHHHHCCCEEEEHHHHCCCCCCCHHHHHHHEEHEEECCCCCCEEEECCCCCCHHHHHHH
IVHRIPPPAPRDTDKLQALIIDSWFDNYLGVVSLVRVMQGEIKPGSKILVMSTGRTHLVD
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHH
KVGVFTPKRKELSALGAGEVGWINASIKDVHGAPVGDTLTLAADPAPHALPGFQEMQPRV
HHCCCCCCHHHHHHCCCCCCEEEECCHHHCCCCCCCCEEEEECCCCCCCCCCHHHHCCHH
FAGLFPVDAEDYPDLREALDKLRLNDAALRFEPESSEAMGFGFRCGFLGMLHMEIVQERL
HCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EREYNLNLISTAPTVVYEVLKTDGSVIPMDNPSKLPPLNNVEEIREPIIRANILTPPDYV
HHHHCCEEEECCHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHH
GNIITLCEEKRGSQIGINYLGSQVQISYELPMAEVVLDFFDKLKSVSRGYASLDYHFLRF
HHHHHHHHHHCCCEEEHEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHCCEEEEEE
DPGPFVRVDTLINGDKVDALSIIVHRSYADRRGRELCEKMKDLIPRQMFDVAIQAAVGSQ
CCCCCEEEEEEECCCCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IISRSTVKAMRKNVLAKCYGGDVSRKKKLLEKQKEGKKRMKQVGRVEIPQEAFLAVLQMD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
K
C
>Mature Secondary Structure 
SSDSMRNIRNFSIIAHVDHGKSTLADRIIQLCGGLQAREMEAQVLDSNPIERERGITIK
CCCHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCHHCCCEEE
AQSVSLPYTAKDGQVYHLNFIDTPGHVDFSYEVSRSLAACEGALLVVDAAQGVEAQSVAN
EEEEECCEECCCCEEEEEEEECCCCCCEEEHHHHHHHHHHCCCEEEEECCCCCCHHHHHH
CYTAVEQGLEVVPVLNKIDLPTADVDRAKAEIEAVIGIDAEDAVAVSAKTGLNIDLVLEA
HHHHHHCCCEEEEHHHHCCCCCCCHHHHHHHEEHEEECCCCCCEEEECCCCCCHHHHHHH
IVHRIPPPAPRDTDKLQALIIDSWFDNYLGVVSLVRVMQGEIKPGSKILVMSTGRTHLVD
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHH
KVGVFTPKRKELSALGAGEVGWINASIKDVHGAPVGDTLTLAADPAPHALPGFQEMQPRV
HHCCCCCCHHHHHHCCCCCCEEEECCHHHCCCCCCCCEEEEECCCCCCCCCCHHHHCCHH
FAGLFPVDAEDYPDLREALDKLRLNDAALRFEPESSEAMGFGFRCGFLGMLHMEIVQERL
HCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
EREYNLNLISTAPTVVYEVLKTDGSVIPMDNPSKLPPLNNVEEIREPIIRANILTPPDYV
HHHHCCEEEECCHHHHHHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHH
GNIITLCEEKRGSQIGINYLGSQVQISYELPMAEVVLDFFDKLKSVSRGYASLDYHFLRF
HHHHHHHHHHCCCEEEHEECCCEEEEEECCCHHHHHHHHHHHHHHHHCCCHHCCEEEEEE
DPGPFVRVDTLINGDKVDALSIIVHRSYADRRGRELCEKMKDLIPRQMFDVAIQAAVGSQ
CCCCCEEEEEEECCCCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IISRSTVKAMRKNVLAKCYGGDVSRKKKLLEKQKEGKKRMKQVGRVEIPQEAFLAVLQMD
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA