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The map label for this gene is mchE [H]

Identifier: 84623334

GI number: 84623334

Start: 1834718

End: 1835962

Strand: Direct

Name: mchE [H]

Synonym: XOO_1677

Alternate gene names: 84623334

Gene position: 1834718-1835962 (Clockwise)

Preceding gene: 84623333

Following gene: 84623335

Centisome position: 37.14

GC content: 64.26

Gene sequence:

>1245_bases
ATGACCCTGTTCCGCGAGGAGGTCATGCAAGCACGCCGCAACAGTTGGCTGGGCGGCATCTCCCTGGCGCAGCCTGTGCG
GGCATGGGTGCTCGCCAGCGCCGCCGGGATCGTGGCGGCATCTGTCGTGGTCTTCCTGTGCATGGGCACCTATGCCCACC
GCTCCACGGTGACCGGACAACTCGTACCGATCAAGGGCCTGGCCACGGTGATGGCGCCGGCCACCGGCGTCGTGAGCCGG
CTGGACGTCAGCGAAGGGCAACCGGTCAAGGCCGGGCAGATGCTCGCCGTGGTCGCGGTTCCGCGCGCGACCCTGGACAG
CGGCGACACGCAGGCGGCACTGCAACACCAATTGCGCAAGCGCGGCGAAGGCGTGAAATCATCCGAGCAAGCGCAGTTGC
AGCAACTGGATGCGCAAGAACAGGGCTTGCGTGCGCAGTTGGCCAATCTGCGCCAGGAATTGATGCAGGTGGACGCAGAA
ATCGCCACCCGTCGCAACCAGATCGCGCTTGCCAATGAGGTGTTGCAACGCTGGCGCCAATTGCAGGACGACAAATACGT
CAGCGTGCTGCAGATCAAGCAGCAGGAATCCACCGCTCTGGAATACACCAGCCAGATGCAGGCCTTGCAGCGGCAGGCGA
CCGAGATGCGACGCAGCGCGGCGCAGATCGAACAGCAACTACGCGTGTTGCCGGGGCAGCGCGGGGGCGTGCAGGCCGAT
TATCGACGGGATGCGGCGGCGGTGGAGCAGGAACAGGTGCAAACCCAGGTCAATGGCGCCTTGGTGGTGACGGCGCCCGT
GGCAGGCGTGGTGGCCACGCAGATGGCAAAACCGGGGCAAGCGATCCAGCTCGGGCAGCCGCTGCTGAGCGTGGTGCCGG
GCGATGGACGCCTGGAAGCCGAATTGCTGGTCCCCAGCCGCGCTATCGGCTTTATCGCCCCAGGCGATACCGTGTTGCTG
CGCTATCAAGCCTTCCCGTACCAGAAGTTTGGTCACCAGCAAGGCCGCCTGAGCCGGATCAGCCGCAGTGCGCTGAGTTC
GAGTGAGTTGGGTGCGTTGATCGGCAATGTCCAGCAGGGCGAACCGTATTACCGGGTAACCGTCGGGCTGGGGCGGCAAA
GTGTCATGGCCTACGGCAAGCCCGAAATGCTCAAGCCGGGGATGCTGTTGCAGGCCGACATCATCGGCGAGCGGAGAAAG
TTGATCGAATGGGTGTTTGAACCATTGTTGTCGCTTGGGAGGTGA

Upstream 100 bases:

>100_bases
TGCCGATCCTGGAGGCAGTGTTGATCGGCACATTCACCATGTGCGCGATGGTGGTGTCCGCTCGCCATGTAACCCGCCGT
ACCGGTCCAGAACACACGCC

Downstream 100 bases:

>100_bases
TCGGTTTGGATGCATCGACGGAAGCGACGCCTGTAATCAAGATGTTCAAGAGTGCTTTGCGAGTGAGATCGCCAGCCAAT
CGCTGACACCTACGGTGCAA

Product: colicin V secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MTLFREEVMQARRNSWLGGISLAQPVRAWVLASAAGIVAASVVVFLCMGTYAHRSTVTGQLVPIKGLATVMAPATGVVSR
LDVSEGQPVKAGQMLAVVAVPRATLDSGDTQAALQHQLRKRGEGVKSSEQAQLQQLDAQEQGLRAQLANLRQELMQVDAE
IATRRNQIALANEVLQRWRQLQDDKYVSVLQIKQQESTALEYTSQMQALQRQATEMRRSAAQIEQQLRVLPGQRGGVQAD
YRRDAAAVEQEQVQTQVNGALVVTAPVAGVVATQMAKPGQAIQLGQPLLSVVPGDGRLEAELLVPSRAIGFIAPGDTVLL
RYQAFPYQKFGHQQGRLSRISRSALSSSELGALIGNVQQGEPYYRVTVGLGRQSVMAYGKPEMLKPGMLLQADIIGERRK
LIEWVFEPLLSLGR

Sequences:

>Translated_414_residues
MTLFREEVMQARRNSWLGGISLAQPVRAWVLASAAGIVAASVVVFLCMGTYAHRSTVTGQLVPIKGLATVMAPATGVVSR
LDVSEGQPVKAGQMLAVVAVPRATLDSGDTQAALQHQLRKRGEGVKSSEQAQLQQLDAQEQGLRAQLANLRQELMQVDAE
IATRRNQIALANEVLQRWRQLQDDKYVSVLQIKQQESTALEYTSQMQALQRQATEMRRSAAQIEQQLRVLPGQRGGVQAD
YRRDAAAVEQEQVQTQVNGALVVTAPVAGVVATQMAKPGQAIQLGQPLLSVVPGDGRLEAELLVPSRAIGFIAPGDTVLL
RYQAFPYQKFGHQQGRLSRISRSALSSSELGALIGNVQQGEPYYRVTVGLGRQSVMAYGKPEMLKPGMLLQADIIGERRK
LIEWVFEPLLSLGR
>Mature_413_residues
TLFREEVMQARRNSWLGGISLAQPVRAWVLASAAGIVAASVVVFLCMGTYAHRSTVTGQLVPIKGLATVMAPATGVVSRL
DVSEGQPVKAGQMLAVVAVPRATLDSGDTQAALQHQLRKRGEGVKSSEQAQLQQLDAQEQGLRAQLANLRQELMQVDAEI
ATRRNQIALANEVLQRWRQLQDDKYVSVLQIKQQESTALEYTSQMQALQRQATEMRRSAAQIEQQLRVLPGQRGGVQADY
RRDAAAVEQEQVQTQVNGALVVTAPVAGVVATQMAKPGQAIQLGQPLLSVVPGDGRLEAELLVPSRAIGFIAPGDTVLLR
YQAFPYQKFGHQQGRLSRISRSALSSSELGALIGNVQQGEPYYRVTVGLGRQSVMAYGKPEMLKPGMLLQADIIGERRKL
IEWVFEPLLSLGR

Specific function: Probably involved, in conjunction with mchF, in the secretion of microcin H47 [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003997
- InterPro:   IPR006143
- InterPro:   IPR006144
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 45102; Mature: 44970

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFREEVMQARRNSWLGGISLAQPVRAWVLASAAGIVAASVVVFLCMGTYAHRSTVTGQ
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LVPIKGLATVMAPATGVVSRLDVSEGQPVKAGQMLAVVAVPRATLDSGDTQAALQHQLRK
EEEHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHCCCCCHHHHHHHHHHH
RGEGVKSSEQAQLQQLDAQEQGLRAQLANLRQELMQVDAEIATRRNQIALANEVLQRWRQ
HCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LQDDKYVSVLQIKQQESTALEYTSQMQALQRQATEMRRSAAQIEQQLRVLPGQRGGVQAD
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
YRRDAAAVEQEQVQTQVNGALVVTAPVAGVVATQMAKPGQAIQLGQPLLSVVPGDGRLEA
HHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCHHCCHHHHHCCCCCCEEE
ELLVPSRAIGFIAPGDTVLLRYQAFPYQKFGHQQGRLSRISRSALSSSELGALIGNVQQG
EEECCCCCCEEECCCCEEEEEEECCCHHHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCC
EPYYRVTVGLGRQSVMAYGKPEMLKPGMLLQADIIGERRKLIEWVFEPLLSLGR
CCEEEEEEECCCHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLFREEVMQARRNSWLGGISLAQPVRAWVLASAAGIVAASVVVFLCMGTYAHRSTVTGQ
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
LVPIKGLATVMAPATGVVSRLDVSEGQPVKAGQMLAVVAVPRATLDSGDTQAALQHQLRK
EEEHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCEEEEEEECCHHCCCCCHHHHHHHHHHH
RGEGVKSSEQAQLQQLDAQEQGLRAQLANLRQELMQVDAEIATRRNQIALANEVLQRWRQ
HCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LQDDKYVSVLQIKQQESTALEYTSQMQALQRQATEMRRSAAQIEQQLRVLPGQRGGVQAD
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
YRRDAAAVEQEQVQTQVNGALVVTAPVAGVVATQMAKPGQAIQLGQPLLSVVPGDGRLEA
HHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCHHCCHHHHHCCCCCCEEE
ELLVPSRAIGFIAPGDTVLLRYQAFPYQKFGHQQGRLSRISRSALSSSELGALIGNVQQG
EEECCCCCCEEECCCCEEEEEEECCCHHHHCCCCHHHHHHHHHHHCHHHHHHHHCCCCCC
EPYYRVTVGLGRQSVMAYGKPEMLKPGMLLQADIIGERRKLIEWVFEPLLSLGR
CCEEEEEEECCCHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11181394 [H]